Urinary oxidative stress biomarker levels and reproductive outcomes among couples undergoing fertility treatments

Abstract STUDY QUESTION Are urinary levels of oxidative stress biomarkers associated with reproductive outcome success following fertility treatments? SUMMARY ANSWER Levels of oxidative stress in the middle tertile for women are associated with the highest levels of reproductive success while no associations were noted for men. WHAT IS KNOWN ALREADY Oxidative stress may contribute to adverse fertility outcomes in the general population, but findings from couples undergoing fertility treatments are sparse. STUDY DESIGN, SIZE, DURATION This prospective cohort study included 481 women and 249 of their male partners undergoing fertility treatments from 2007 to 2015, from the Environment and Reproductive Health (EARTH) study in Boston, MA. PARTICIPANTS/MATERIALS, SETTING, METHODS One urine sample per participant was collected at each cycle and analysed for two oxidative stress markers: 8-isoprostane-PGF2α (8-iso-PGF2α) and 8-isoprostane-PGF2α metabolite (F2-isoP-M). Reproductive outcomes were abstracted from medical records and included the fertilization rate, for IVF (oocytes fertilized/mature oocytes retrieved), and rates of implantation, clinical pregnancy and live birth, for both IVF and IUI. Cluster-weighted generalized estimating equations were used to analyse adjusted associations between exposure tertiles and outcomes. MAIN RESULTS AND THE ROLE OF CHANCE Levels of F2-isoP-M in the middle tertile were associated with the most success among women. Women in the upper tertile of F2-isoP-M had an adjusted mean live birth rate after IVF and IUI of 23% (95% CI: 17, 29) compared to 38% (95% CI: 31, 45) for women in the middle tertile and 27% (95% CI: 21, 34) in the lower tertile. The fertilization rate during IVF was higher for women with 8-iso-PGF2α in the middle tertile (0.77 [95% CI: 0.73, 0.80]) compared to women in the lower (0.69 [95% CI: 0.64, 0.73]) or upper tertiles (0.66 [95% CI: 0.61, 0.71]). No significant associations were found for other measured outcomes with 8-iso-PGF2α, or between any oxidative stress biomarker in men and reproductive outcomes in their partners. LIMITATIONS, REASONS FOR CAUTION Isoprostanes are short-lived biomarkers and this study may not have captured the most relevant window of susceptibility for oxidative stress on the outcomes of interest. Findings from this study may not be generalizable to couples attempting conception without fertility assistance. WIDER IMPLICATIONS OF THE FINDINGS This study suggests that a non-linear association may exist between oxidative stress and reproductive outcomes in a population undergoing fertility treatment, a finding not previously identified in the literature. Oxidative stress may represent the mechanism through which environmental chemicals are associated with adverse reproductive outcomes. STUDY FUNDING/COMPETING INTEREST(S) This research was supported by the Intramural Research Program of the National Institutes of Environmental Health Sciences (NIEHS) (ZIA ES103314) and by NIEHS grants R01ES022955, R01ES009718 and R01ES00002. There are no competing interests to report. TRIAL REGISTRATION NUMBER N/A

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Administration of Purwoceng (Pimpinella alpina Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

Journal of Natural Remedies ◽

10.18311/jnr/2016/7684 ◽

2016 ◽

Vol 16 (3) ◽

pp. 115

Author(s):

Taufiqurrachman Nasihun ◽

Eni Widayati

Keyword(s):

Oxidative Stress ◽

Male Rats ◽

Aging Male ◽

Sprague Dawley ◽

Oxidative Stress Biomarkers ◽

Frap Assay ◽

Stress Biomarkers ◽

Post Hoc ◽

Stress Biomarker ◽

Oxidative Stress Biomarker

Introduction: UVC may induce oxidative stress mediated by ROS. Pimpinella alpina Molk (PaM) have been utilized by aging male as rejuvenation remedy. Objective: To elucidate the PaM effect on oxidative stress biomarker in rats. Methods: 25 of Sprague-Dawley male rats were divided into 5 groups, PaM with a dosage of 50, 100, and 150 mg were administered during 15 days to evaluate the biomarker of oxidative stress following UVC irradiation for 30 minutes beforehand. The oxidative stress biomarkers were measured by TBARS and FRAP assay respectively. Results: Post Hoc analysis demonstrated that MDA and 8-OHdG concentration in PaM group were significantly lower (p < 0.001), otherwise the concentration of TAC in PaM group were significantly higher, p < 0.001. Conclusion: Administration of PaM with 50, 100, and 150 mg dosage capable of improving oxidative stress biomarker in Sprague-Dawley male rats.

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Monitoring of Urinary Oxidative Stress Biomarker Levels in Newborns with Hypoxic-Ischemic Encephalopathy

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2020.10.291 ◽

2020 ◽

Vol 159 ◽

pp. S115

Author(s):

Mari Merce Cascant Vilaplana ◽

Ángel Sánchez-Illana ◽

José David Piñeiro-Ramos ◽

Guillermo Quintás ◽

Camille Oger ◽

...

Keyword(s):

Oxidative Stress ◽

Hypoxic Ischemic Encephalopathy ◽

Ischemic Encephalopathy ◽

Stress Biomarker ◽

Oxidative Stress Biomarker

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Effects of Phenytoin on Glutathione Status and Oxidative Stress Biomarker Gene mRNA Levels in Cultured Precision Human Liver Slices

Toxicological Sciences ◽

10.1093/toxsci/59.1.118 ◽

2001 ◽

Vol 59 (1) ◽

pp. 118-126 ◽

Cited By ~ 25

Author(s):

E. P. Gallagher

Keyword(s):

Oxidative Stress ◽

Human Liver ◽

Mrna Levels ◽

Glutathione Status ◽

Liver Slices ◽

Human Liver Slices ◽

And Oxidative Stress ◽

Stress Biomarker ◽

Oxidative Stress Biomarker ◽

Gene Mrna

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Detection of Oxidative Stress Biomarker Homocysteine Utilizing Resonance Elastic Light Scattering

ECS Transactions ◽

10.1149/1.3491284 ◽

2019 ◽

Vol 28 (18) ◽

pp. 115-128

Author(s):

Magdalena Stobiecka ◽

Sara Cutler ◽

Zachary Reed ◽

Amanda Prance ◽

Maria Hepel

Keyword(s):

Oxidative Stress ◽

Light Scattering ◽

Elastic Light Scattering ◽

Stress Biomarker ◽

Oxidative Stress Biomarker

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P–708 Comparison of reproductive outcomes after progestin-primed ovarian stimulation with dydrogesterone versus cetrorelix to inhibit spontaneous ovulation in oocyte donation

Human Reproduction ◽

10.1093/humrep/deab130.707 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

J A Moreno ◽

P Masoli ◽

C Sferrazza ◽

H Leiva ◽

O Espinosa ◽

...

Keyword(s):

Pregnancy Rate ◽

Live Birth ◽

Ovarian Stimulation ◽

Fertilization Rate ◽

Oocyte Donation ◽

Pregnancy Rates ◽

Clinical Pregnancy ◽

Reproductive Outcomes ◽

Ongoing Pregnancy ◽

Live Births

Abstract Study question Is dydrogesterone (DYG) equivalent compared to cetrorelix with respect to clinical pregnancy rate, ongoing pregnancy rate and live birth rate in oocyte donation (OD) cycles? Summary answer DYG is comparable to cetrorelix in terms of clinical pregnancy, but higher rates of ongoing pregnancy and live birth were observed in the DYG group What is known already Progestin-primed ovarian stimulation (PPOS) is an ovarian stimulation regimen based on a freeze-all strategy using progestin as an alternative to GnRH analog for suppressing a premature LH surge. DYG is an oral progestin that has been studied in PPOS protocols. Published reports indicate that length of ovarian stimulation, dose of gonadotrophin needed and number of MII retrieved from PPOS cycles are comparable to short protocol of GnRH agonists during OD cycles. However, while some studies noted no differences in terms of live births, worse pregnancy rates have been reported in recipients of oocytes from PPOS cycles compared to GnRH antagonists. Study design, size, duration Prospective controlled study to assess the reproductive outcomes of OD recipients in which the donors were subjected to the DYG protocol (20mg/day) compared with those subjected to the short protocol with cetrorelix (0.25 mg/day) from Day 7 or since a leading follicle reached 14 mm. The OD cycles were triggered with triptoreline acetate and the trigger criterion was ≥3 follicles of diameter >18mm. Participants/materials, setting, methods 202 oocyte donors were included, 92 under DYG and 110 under cetrorelix. The study was performed in a private infertility center between January 2017 and December 2020. The main outcome included the rates of clinical pregnancy, ongoing pregnancy and live births. Secondary outcomes included the number of oocytes retrieved, number of MII, fertilization rate, length of stimulation and total gonadotropin dose. Differences were tested using a Student’s t-test or a Chi2 test, as appropriate. Main results and the role of chance Compared to antagonist cycles, cycles under DYG had fewer days of stimulation (9.9 ± 0.9 vs. 10.8 ± 1.1, p<.001) and a lower total gonadotropin dose (1654 ± 402.4 IU vs. 1844 ± 422 IU, p<.001). The number of MII retrieved was no different: 16.9 (SD 6.2) with DYG and 15.4 (SD 5.8) with cetrorelix (p = 0.072). Recipients and embryo transfer (ET) characteristics were also similar between groups. The mean number of MII assigned to each recipients was 6.7 (SD 1.8) in DYG and 6.6 (SD 1.7) in cetrorelix (P = 0.446). The fertilization rate was 66.2% in DYG versus 67.6% in cetrorelix (P = 0.68). Regarding the reproductive outcomes, the overall clinical pregnancy rate in DYG group (65/87: 74.7%) and cetrorelix group (66/104: 63.4%) (p = 0.118) was similar. Meanwhile, the DYG group compared to cetrorelix group had higher rates of ongoing pregnancy (63.2% vs 45.1%; p = 0.014) and live births (54,9% vs 37.8%; p = 0.040). Limitations, reasons for caution These results should be evaluated with caution. The limitations of this study include the limited number of participants enrolled and the limited data on pregnancy outcomes. A randomized controlled trial is necessary to provide more evidence on the efficacy of the DYG protocol. Wider implications of the findings: The efficacy of PPOS protocol compared to GnRH-antagonist protocol in terms of reproductive outcomes has been little studied. PPOS using DYG yields comparable clinical pregnancy rates compared to cetrorelix in OD cycles. The differences found regarding the rates of ongoing pregnancy and live births should be further investigated. Trial registration number Not applicable

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