Genetic characterization of wild and farmed European seabass in the Adriatic sea: assessment of farmed escapees using a Bayesian approach

2016 ◽  
Vol 74 (1) ◽  
pp. 369-378 ◽  
Author(s):  
Tanja Šegvić-Bubić ◽  
Leon Grubišić ◽  
Željka Trumbić ◽  
Rino Stanić ◽  
Jelena Ljubković ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Adriatic Sea ◽  
Dicentrarchus Labrax ◽  
Population Subdivision ◽  
Wild Populations ◽  
Fish Farms ◽  
Effective Population ◽  
Farmed Fish ◽  
European Seabass ◽  
Solid Foundation

Hybridizations between domesticated escaped farmed fish and wild conspecifics may increase genetic diversity or break down locally adapted gene complexes, thus reducing competitive ability and overall fitness. We examined the genetic structure of six farmed populations of European seabass, Dicentrarchus labrax, of different geographical origin, and ten neighbouring wild populations in the Adriatic Sea using 10 microsatellite loci. All loci were polymorphic, with mean expected heterozygosity >74% in all 16 populations. Overall number of alleles as well as short-term effective population size were smaller in farmed (A = 18.7; Ne = 56.1) than in wild populations (A = 21.2; Ne = 180.6). The global FST of 0.0672 across loci showed significant population subdivision. Strong genetic differences between farmed fish and their wild conspecifics enabled the identification of seabass escapees back to their origins and the estimation of the extent of hybridization. Bayesian assignment analyses clustered wild populations together, whereas each farmed populations was assigned to a separate cluster. Intraspecific hybridization was highest in wild populations in areas impacted by fish farms, where highly admixed populations had decreased genetic diversity. The results of this study represent a solid foundation required to establish a genetic register of European seabass wild and farmed populations in the eastern Adriatic Sea that are required to establish a seabass DNA register.

scholarly journals Mycobacterium pseudoshottsii in Mediterranean Fish Farms: New Trouble for European Aquaculture?

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 610
Author(s):  
Davide Mugetti ◽  
Katia Varello ◽  
Andrea Gustinelli ◽  
Paolo Pastorino ◽  
Vasco Menconi ◽  
...  
Keyword(s):  
Fish Species ◽  
Sparus Aurata ◽  
Dicentrarchus Labrax ◽  
Housekeeping Genes ◽  
The United States ◽  
Sciaenops Ocellatus ◽  
Fish Farms ◽  
Farmed Fish ◽  
Potential Threat ◽  
European Aquaculture

Mycobacterium pseudoshottsii, a slow-growing mycobacterium closely related to M. marinum, has been isolated only in wild fish in the United States and in Japanese fish farms to date. Here, we report cases of mortality in three farmed fish species (Dicentrarchus labrax, Sparus aurata, and Sciaenops ocellatus) caused by M. pseudoshottsii in Italy. Samples underwent necropsy, histology, and culture with pathogen identification based on PCR and sequencing of housekeeping genes (16S rRNA, hsp65, rpoB). Multifocal to coalescing granulomatous and necrotizing inflammation with acid-fast bacilli were observed in the parenchymatous organs, from which M. pseudoshottsii was isolated and identified. Phylogenetic analysis confirmed the results of gene sequencing and allowed subdivision of the isolates into three distinct groups. M. pseudoshottsii poses a potential threat for Mediterranean aquaculture. Its origin in the area under study needs to be clarified, as well as the threat to the farmed fish species.

scholarly journals Vertebrae Morphometric Measurement and Ca/P Levels of Different Age European Seabass (Dicentrarchus labrax)

Fishes ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 37
Author(s):  
Vasiliki Nikiforidou ◽  
Stefanos Zaoutsos ◽  
Nikolaos Vlahos ◽  
Panagiotis Berillis
Keyword(s):  
Vertebral Column ◽  
Age Groups ◽  
Dicentrarchus Labrax ◽  
Morone Saxatilis ◽  
Morphometric Measurement ◽  
Fish Farms ◽  
Important Species ◽  
X Ray ◽  
The Third ◽  
European Seabass

The European seabass is one of the most important species of the Mediterranean, specifically Greece. Individuals with different numbers of vertebrae have been reported. This number ranges from 24 to 26 vertebrae. In this study a sample of 73 individual seabass were collected from fish farms and divided into three age groups. The first group included fingerling individuals, the second group, juvenile individuals and the third group, adult individuals. The number and the length of their vertebrae were measured by radiographs. The individuals were divided into subgroups according to their vertebrae number, and from each one the tenth vertebra was taken. Ca and P levels (%) of each tenth vertebra were measured by X-ray spectroscopy (EDS), and the Ca/P ratio was determined. Vertebrae length, Ca and P levels and Ca/P ratio were compared among age groups and among individuals with different numbers of vertebrae. It was shown that the European seabass’s vertebral column can be divided to three sections—cervical, abdominal and caudal—following the striped bass (Morone saxatilis) model.

Genetic diversity maintained in comparison of captive-propagated and wild populations of Lampsilis fasciola and Ptychobranchus fasciolaris (Bivalvia: Unionidae)

2021 ◽  
Author(s):  
Nichelle M. VanTassel ◽  
Todd J. Morris ◽  
Christopher G. Wilson ◽  
David T Zanatta
Keyword(s):  
Genetic Diversity ◽  
Wild Population ◽  
Strong Support ◽  
Wild Populations ◽  
Effective Population ◽  
Brood Stock ◽  
Genetic Population ◽  
Captive Populations ◽  
Population Structure Analysis ◽  
Genetic Diversity And Structure

We compared the genetic diversity and structure between wild and captive-propagated freshwater mussels at risk in Canada, Lampsilis fasciola (Wavy-rayed Lampmussel, WRL) and Ptychobranchus fasciolaris (Kidneyshell, KS), using microsatellite generated genotypes. No significant differences were detected between wild and propagated WRL (from 12 mothers) or KS (from 7 mothers) based on genetic diversity metrics using Kruskal-Wallis tests. Effective population size (Ne) was estimated and there was considerable overlap in the estimated Ne between wild and captive populations of both species. Pairwise FST and Dest values among wild WRL, hatchery-raised WRL juveniles, and across different time periods (2008 to 2018) were generally low (FST = 0.007-0.043, Dest = 0.005-0.282). Pairwise FST and Dest for KS were not significant and low (0.012, 0.017). When comparing captive-reared offspring with the wild population, STRUCTURE analysis revealed strong support for a single genetic population. With at least 7 females contributing to brood stock, genetic diversity was maintained. Further research is needed to assess the male contribution to each glochidial brood, and the variability of genetic diversity in individual glochidial broods.

scholarly journals Genetic structuring in farmed and wild Gilthead seabream and European seabass in the Mediterranean Sea: implementations for detection of escapees

2020 ◽  
Vol 33 ◽  
pp. 7
Author(s):  
Eirini-Slavka Polovina ◽  
Evelina Kourkouni ◽  
Costas S. Tsigenopoulos ◽  
Pablo Sanchez-Jerez ◽  
Emmanuel D. Ladoukakis
Keyword(s):  
Mediterranean Sea ◽  
Sparus Aurata ◽  
Eastern Mediterranean ◽  
Dicentrarchus Labrax ◽  
Western Mediterranean ◽  
Gilthead Seabream ◽  
Fish Farms ◽  
Genetic Structuring ◽  
European Seabass ◽  
The Mediterranean

Microsatellite markers were used to investigate the genetic structure of the two most important cultured fish in the Mediterranean Sea, the gilthead seabream (Sparus aurata) and the European seabass (Dicentrarchus labrax), from two (one wild and one farmed) populations in Western Mediterranean (Spain) and from two (one wild and one farmed) populations Eastern Mediterranean (Greece). All populations were in Hardy-Weinberg disequilibrium. Interestingly, wild and farmed populations for both species from Greece were genetically differentiated and could be distinguished from each other. We used Bayesian methods for cluster analysis of farmed and wild populations. Our analysis has implications for the identification of escapees from fish farms to the wild.

Genetic diversity among five wild populations of Portunus trituberculatus

2013 ◽  
Vol 18 (6) ◽  
pp. 1327-1334
Author(s):  
Xiaoping LI ◽  
Ping LIU ◽  
Jian LI ◽  
Baoquan GAO
Keyword(s):  
Genetic Diversity ◽  
Portunus Trituberculatus ◽  
Wild Populations

Microsatellite analysis of genetic diversity in 3 wild populations of Exopalaemon carinicauda

2012 ◽  
Vol 36 (12) ◽  
pp. 1819
Author(s):  
Shuwen JIA ◽  
Ping LIU ◽  
Jian LI ◽  
Jitao LI ◽  
Baoquan GAO ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Analysis ◽  
Exopalaemon Carinicauda ◽  
Wild Populations

Microsatellite analysis on genetic diversity of seven wild populations of Scylla paramamosain in China

2012 ◽  
Vol 35 (7) ◽  
pp. 977-984 ◽  
Author(s):  
Miao-an SHU ◽  
Yu-fang ZHOU ◽  
Xiao-yu ZHU ◽  
Xiao-feng ZHAO ◽  
Xiao-ling GUO
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Analysis ◽  
Scylla Paramamosain ◽  
Wild Populations

Evolutionary genetics of small populations

2017 ◽  
Author(s):  
Richard Frankham ◽  
Jonathan D. Ballou ◽  
Katherine Ralls ◽  
Mark D. B. Eldridge ◽  
Michele R. Dudash ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Size ◽  
Evolutionary Genetics ◽  
Small Population ◽  
Small Populations ◽  
Effective Population ◽  
Genetic Management ◽  
Evolutionary Genetic ◽  
Loss Of Genetic Diversity ◽  
Number Of Individuals

Genetic management of fragmented populations involves the application of evolutionary genetic theory and knowledge to alleviate problems due to inbreeding and loss of genetic diversity in small population fragments. Populations evolve through the effects of mutation, natural selection, chance (genetic drift) and gene flow (migration). Large outbreeding, sexually reproducing populations typically contain substantial genetic diversity, while small populations typically contain reduced levels. Genetic impacts of small population size on inbreeding, loss of genetic diversity and population differentiation are determined by the genetically effective population size, which is usually much smaller than the number of individuals.

scholarly journals Trends in genetic diversity and the effect of inbreeding in American Angus cattle under genomic selection

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Emmanuel A. Lozada-Soto ◽  
Christian Maltecca ◽  
Duc Lu ◽  
Stephen Miller ◽  
John B. Cole ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Beef Cattle ◽  
Genomic Selection ◽  
Inbreeding Depression ◽  
Growth Traits ◽  
Effective Population ◽  
Angus Cattle ◽  
Genomic Inbreeding ◽  
Chromosome Segments ◽  
The Impact

Abstract Background While the adoption of genomic evaluations in livestock has increased genetic gain rates, its effects on genetic diversity and accumulation of inbreeding have raised concerns in cattle populations. Increased inbreeding may affect fitness and decrease the mean performance for economically important traits, such as fertility and growth in beef cattle, with the age of inbreeding having a possible effect on the magnitude of inbreeding depression. The purpose of this study was to determine changes in genetic diversity as a result of the implementation of genomic selection in Angus cattle and quantify potential inbreeding depression effects of total pedigree and genomic inbreeding, and also to investigate the impact of recent and ancient inbreeding. Results We found that the yearly rate of inbreeding accumulation remained similar in sires and decreased significantly in dams since the implementation of genomic selection. Other measures such as effective population size and the effective number of chromosome segments show little evidence of a detrimental effect of using genomic selection strategies on the genetic diversity of beef cattle. We also quantified pedigree and genomic inbreeding depression for fertility and growth. While inbreeding did not affect fertility, an increase in pedigree or genomic inbreeding was associated with decreased birth weight, weaning weight, and post-weaning gain in both sexes. We also measured the impact of the age of inbreeding and found that recent inbreeding had a larger depressive effect on growth than ancient inbreeding. Conclusions In this study, we sought to quantify and understand the possible consequences of genomic selection on the genetic diversity of American Angus cattle. In both sires and dams, we found that, generally, genomic selection resulted in decreased rates of pedigree and genomic inbreeding accumulation and increased or sustained effective population sizes and number of independently segregating chromosome segments. We also found significant depressive effects of inbreeding accumulation on economically important growth traits, particularly with genomic and recent inbreeding.

Sign in / Sign up

Export Citation Format

Share Document