Genetic diversity maintained in comparison of captive-propagated and wild populations of Lampsilis fasciola and Ptychobranchus fasciolaris (Bivalvia: Unionidae)

2021 ◽  
Author(s):  
Nichelle M. VanTassel ◽  
Todd J. Morris ◽  
Christopher G. Wilson ◽  
David T Zanatta
Keyword(s):  
Genetic Diversity ◽  
Wild Population ◽  
Strong Support ◽  
Wild Populations ◽  
Effective Population ◽  
Brood Stock ◽  
Genetic Population ◽  
Captive Populations ◽  
Population Structure Analysis ◽  
Genetic Diversity And Structure

We compared the genetic diversity and structure between wild and captive-propagated freshwater mussels at risk in Canada, Lampsilis fasciola (Wavy-rayed Lampmussel, WRL) and Ptychobranchus fasciolaris (Kidneyshell, KS), using microsatellite generated genotypes. No significant differences were detected between wild and propagated WRL (from 12 mothers) or KS (from 7 mothers) based on genetic diversity metrics using Kruskal-Wallis tests. Effective population size (Ne) was estimated and there was considerable overlap in the estimated Ne between wild and captive populations of both species. Pairwise FST and Dest values among wild WRL, hatchery-raised WRL juveniles, and across different time periods (2008 to 2018) were generally low (FST = 0.007-0.043, Dest = 0.005-0.282). Pairwise FST and Dest for KS were not significant and low (0.012, 0.017). When comparing captive-reared offspring with the wild population, STRUCTURE analysis revealed strong support for a single genetic population. With at least 7 females contributing to brood stock, genetic diversity was maintained. Further research is needed to assess the male contribution to each glochidial brood, and the variability of genetic diversity in individual glochidial broods.

scholarly journals ISSR-based genetic diversity of wild and ex situ conserved population of Hopea chinensis (Merr.) Hand.-Mazz

2018 ◽  
Vol 44 (3) ◽  
pp. 407-413
Author(s):  
Wen-Xiu Tang ◽  
Wan-Juan Dai ◽  
Xing-Hua Hu ◽  
Shi-Xun Huang
Keyword(s):  
Genetic Diversity ◽  
Wild Population ◽  
Diversity Index ◽  
Ex Situ ◽  
Polymorphic Band ◽  
High Genetic Diversity ◽  
Average Value ◽  
Information Index ◽  
Population Structure Analysis ◽  
Genetic Diversity And Structure

The genetic diversity and structure of 4 wild population and 2 ex situ conserved population of Hopea chinensis (Merr.) Hand.-Mazz were analyzed and compared by ISSR marker method. Fifty eight bands were amplified from total genomic DNA with 10 primers in which there were 51 polymorphic, the percentage of polymorphic bands of populations ranged from 53.45 to 79.31, with an average value of 69.82, indicating the presence of high genetic diversity. However, the percentage of polymorphic band, Nei’s diversity index (He) and Shannon information index (I) of wild populations (86.21, 0.3636 and 0.5232, respectively) were higher than those of ex situ conservated populations (77.59, 0.3153 and 0.4597, respectively), which meant that ex situ conservations of Hopea chinensis had a narrow genetic diversity. Population structure analysis revealed that genetic variation of Hopea chinensis was 0.0373, so the genetic differentiation was 3.73% among populations, and 96.27% in populations. Furthermore, the strong gene flow (Nm = 12.8896) would be the main reason of limited population differentiation. It concluded that ex situ population had not yet fully covered the entire genetic diversity of Hopea chinensis.  

scholarly journals Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6643
Author(s):  
Guoling Chen ◽  
Chenqing Zheng ◽  
Nelson Wan ◽  
Daoqiang Liu ◽  
Vivian Wing Kan Fu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Haplotype Analysis ◽  
Genetic Relatedness ◽  
Wild Population ◽  
Critically Endangered ◽  
Assignment Test ◽  
Microsatellite Data ◽  
Wild Populations ◽  
Captive Populations ◽  
Mitochondrial Data

Background Captive populations permit research and conservation of endangered species in which these efforts are hardly implemented in wild populations. Thus, analysing genetic diversity and structure of captive populations offers unique opportunities. One example is the critically endangered Blue-crowned Laughingthrush, Garrulax courtoisi, which has only two known wild populations in Wuyuan, Jiangxi and Simao, Yunnan, China. We carried out the first conservation genetic study, in order to provide useful implications that allow for successful ex situ conservation and management of the Blue-crowned Laughingthrush. Methods Using the novel microsatellite markers developed by whole-genome sequencing, we genotyped two captive populations, from the Ocean Park Hong Kong, which are of unknown origin, and the Nanchang Zoo, which were introduced from the Wuyuan wild population since the year 2010–2011, respectively. The genetic diversity of captive Blue-crowned Laughingthrush populations was estimated based on genetic polymorphisms revealed by a new microsatellite data set and mitochondrial sequences. Then, we characterised the population structure using STRUCTURE, principal coordinates analysis, population assignment test using the microsatellite data, and haplotype analysis of mitochondrial data. Additionally, we quantified genetic relatedness based on the microsatellite data with ML-Relate. Results Our results showed equally low levels of genetic diversity of the two captive Blue-crowned Laughingthrush populations. The population structure analysis, population assignment test using the microsatellite data, and haplotype analysis of the mitochondrial data showed weak population structuring between these two populations. The average pairwise relatedness coefficient was not significant, and their genetic relatedness was quantified. Discussion This study offers a genetic tool and consequently reveals a low level of genetic diversity within populations of a critically endangered bird species. Furthermore, our results indicate that we cannot exclude the probability that the origin of the Hong Kong captive population was the wild population from Wuyuan. These results provide valuable knowledge that can help improve conservation management and planning for both captive and wild Blue-crowned Laughingthrush populations.

scholarly journals Cross Species/Genera Transferability of SSR Markers, Genetic Diversity and Population Structure Analysis in Gladiolus (Gladiolus × grandiflorus L.) Genotypes

2021 ◽  
Author(s):  
Varun Hiremath ◽  
Kanwar Pal Singh ◽  
Neelu Jain ◽  
Kishan Swaroop ◽  
Pradeep Kumar Jain ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Structure Analysis ◽  
Genetic Relationships ◽  
Crop Improvement ◽  
Test Analysis ◽  
Average Value ◽  
Population Structure Analysis ◽  
Genetic Diversity And Structure

Abstract Genetic diversity and structure analysis using molecular markers is necessary for efficient utilization and sustainable management of gladiolus germplasm. Genetic analysis of gladiolus germplasm using SSR markers is largely missing due to scarce genomic information. In the present investigation, we report 66.66% cross transferability of Gladiolus palustris SSRs whereas 48% of Iris EST-SSRs were cross transferable across the gladiolus genotypes used in the study. A total of 17 highly polymorphic SSRs revealed a total 58 polymorphic loci ranging from two to six in each locus with an average of 3.41 alleles per marker. PIC values ranged from 0.11 to 0.71 with an average value of 0.48. Four SSRs were selectively neutral based on Ewens-Watterson test. Analysis of genetic structure of 84 gladiolus genotypes divided whole germplasm into two subpopulations. 35 genotypes were assigned to subpopulation 1 whereas 37 to subpopulation 2 and rest of the genotypes recorded as admixture. Analysis of molecular variance indicated maximum variance (53.59%) among individuals within subpopulations whereas 36.55% of variation observed among individuals within total population. Least variation (9.86%) was noticed between two subpopulations. Moderate (FST = 0.10) genetic differentiation of two subpopulations was observed. Grouping pattern of population structure was consistent with UPGMA dendrogram based on simple matching dissimilarity coefficient (ranged from 01.6 to 0.89) and PCoA. Genetic relationships assessed among the genotypes of respective clusters assist the breeders in selecting desirable parents for crossing. SSR markers from present study can be utilized for cultivar identification, conservation and sustainable utilization of gladiolus genotypes for crop improvement.

scholarly journals Genetic diversity, structure and gene flow of migratory barren-ground caribou (Rangifer tarandus groenlandicus) in Canada

Rangifer ◽  
2016 ◽  
Vol 36 (1) ◽  
pp. 1 ◽  
Author(s):  
Keri McFarlane ◽  
Anne Gunn ◽  
Mitch Campbell ◽  
Mathieu Dumond ◽  
Jan Adamczewski ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gene Flow ◽  
Rangifer Tarandus ◽  
Continuous Distribution ◽  
Effective Population ◽  
Genetic Population ◽  
Barren Ground ◽  
Cyclic Changes ◽  
Rangifer Tarandus Groenlandicus

Migratory barren-ground caribou (Rangifer tarandus groenlandicus) provide an opportunity to examine the genetic population structure of a migratory large mammal whose movements and distribution, in some instances, have not been heavily influenced by human activities that result in habitat loss or fragmentation. These caribou have likely reached large effective population sizes since their rapid radiation during the early Holocene despite cyclic changes in abundance. Migratory barren-ground caribou are managed as discrete subpopulations. We investigated genetic variation among those subpopulations to determine the patterns of genetic diversity within and among them, and the implications for long-term persistence of caribou. We identified three distinct genetic clusters across the Canadian arctic tundra: the first cluster consisted of all fully-continental migratory barren-ground subpopulations; the second cluster was the Dolphin and Union caribou; and the third cluster was caribou from Southampton Island. The Southampton Island caribou are especially genetically distinct from the other barren-ground type caribou. Gene flow among subpopulations varied across the range. Occasional gene flow across the sea-ice is likely the reason for high levels of genetic variation in the Dolphin and Union subpopulation, which experienced very low numbers in the past. These results suggest that for most migratory caribou subpopulations, connectivity among subpopulations plays an important role in maintaining natural genetic diversity. Our analyses provide insight into the levels of microsatellite genetic diversity and patterns of gene flow that may be common to large subpopulations that historically had a continuous distribution across a large continental range. These data can also be used as a benchmark to compare the effects of habitat fragmentation and bottlenecks on other large caribou populations.

scholarly journals Genetic population structure of the toxigenic dinoflagellate Alexandrium catenella in the Patagonian Fjords System, southern Chile

2020 ◽  
Vol 55 (1) ◽  
pp. 26
Author(s):  
Javier Paredes-Mella ◽  
Daniel Varela
Keyword(s):  
Genetic Diversity ◽  
First Record ◽  
Southern Chile ◽  
Genetic Population ◽  
Vegetative Cells ◽  
Alexandrium Catenella ◽  
Diversity Gradient ◽  
The Pacific ◽  
Genetic Diversity And Structure ◽  
High Gene

Alexandrium catenella is the main species that form harmful algae blooms (HABs) in southern Chile. Since its first record in 1972 in the Magallanes region this species apparently has increased its range distribution from south to north. In this study, we investigate the influence of the range expansions distribution on the A. catenella populations genetic diversity and structure. This was achieved by isolating 33 clones from different localities along the Magallanes and Aysén region which were genetically characterized with Amplified Fragment Length Polymorphism (AFLPs) molecular markers. Results showed a latitudinal genetic diversity gradient from the south to north populations. Inter-populations genetic divergences were low but significant between both geographically close and distant populations. Results indicated that the genetic diversity differentiation could be generated by a founder effect, which is expected in populations that have expanded their distribution range. On the other hand, low levels of genetic divergences between distant populations seems point out that high gene flow occurs along coast of the Pacific Ocean, but also, seems hints the connectivity route between the Magallanes and Aysén populations i.e., the vegetative cells dispersion among populations, would occur through coastal Pacific coast and Boca del Guafo. Finally, the high values of multilocus linkage disequilibrium found between closer population of Aysén indicates that divergence could be influenced along with the reproductive dynamic of the vegetative cells.

scholarly journals Anthropogenic river fragmentation reduces long-term viability of the migratory fish Salminus brasiliensis (Characiformes: Bryconidae) populations

2021 ◽  
Vol 19 (2) ◽  
Author(s):  
Josiane Ribolli ◽  
Evoy Zaniboni-Filho ◽  
Carolina Barros Machado ◽  
Tailise Carolina de Souza Guerreiro ◽  
Patrícia Domingues de Freitas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Anthropogenic Impacts ◽  
Species Conservation ◽  
Migratory Fish ◽  
Effective Population ◽  
Long Distance ◽  
Management Actions ◽  
Population Structure Analysis ◽  
Salminus Brasiliensis

Abstract Life-history, geographical barriers, and damming can shape the genetic diversity of freshwater migratory fish, which are particularly vulnerable to anthropogenic impacts. We investigated the genetic diversity of Salminus brasiliensis, a long-distance migratory species that is recognized as an important provider of ecosystem services. We implemented microsatellite analyses to assess genetic diversity and simulate future scenarios for evaluating the long-term viability of dammed and non-dammed populations from the Uruguay River. High levels of genetic diversity were detected for all sampled populations. However, effective population sizes were lower in the uppermost river stretches, where the landscape is highly fragmented. Population structure analysis indicated two spatial genetic populations. It is suggested that this genetic structure preserves populations partially isolated by an ancient natural barrier, instead of being a result of the presence of dams. The simulated genetic scenarios indicated that genetic variability of S. brasiliensis populations from upstream dams could collapse over the years, mainly due to the reduction in the number of alleles. Therefore, besides helping to better understand issues related to the influence of dams on the genetic diversity of migratory fish, our results are especially relevant for driving local fishery policies and management actions for the species conservation.

Absence of genetic structure reflects post-glacial history and present-day host use in Mapleleaf (Quadrula quadrula) mussel from Manitoba, Canada

2020 ◽  
Vol 98 (8) ◽  
pp. 551-556
Author(s):  
N.M. VanTassel ◽  
C.E. Beaver ◽  
D.A. Watkinson ◽  
T.J. Morris ◽  
D.T. Zanatta
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Great Lakes ◽  
Host Use ◽  
Ohio River ◽  
Glacial History ◽  
Genetic Population ◽  
Genetic Diversity And Structure ◽  
Declining Populations ◽  
River Drainages

Our study documents and analyzes the absence of genetic diversity and structure of the Mapleleaf (Quadrula quadrula (Rafinesque, 1820)) (Bivalvia: Unionidae) mussel in the Lake Winnipeg, Assiniboine River, and Red River drainages (Manitoba, Canada). Previous studies have revealed patterns of genetic diversity and structure in the Mississippi and Ohio river drainages, as well as in the Laurentian Great Lakes drainage. Genotypes from six variable microsatellite loci showed that the Q. quadrula population in Manitoba was significantly differentiated from the population in the Great Lakes drainage (Ontario, Canada), supporting the existence of two Designatable Units in Canada. Conversely, there was no evidence of genetic structure within the sampled range of Q. quadrula in Manitoba. The lack of genetic structure in Q. quadrula across its distribution in Manitoba reflected its post-glacial history and use of a vagile host and necessitates that efforts should be made to ensure connectivity and maintain gene flow across the region. Given that the evidence suggests that Manitoba Q. quadrula belong to a single genetic population, movement of hatchery-propagated juvenile Q. quadrula, adult Q. quadrula, or glochidia-carrying host catfish sourced from any location in Manitoba could be used to augment declining populations or at-risk locations in Manitoba.

Genetic characterization of wild and farmed European seabass in the Adriatic sea: assessment of farmed escapees using a Bayesian approach

2016 ◽  
Vol 74 (1) ◽  
pp. 369-378 ◽  
Author(s):  
Tanja Šegvić-Bubić ◽  
Leon Grubišić ◽  
Željka Trumbić ◽  
Rino Stanić ◽  
Jelena Ljubković ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Adriatic Sea ◽  
Dicentrarchus Labrax ◽  
Population Subdivision ◽  
Wild Populations ◽  
Fish Farms ◽  
Effective Population ◽  
Farmed Fish ◽  
European Seabass ◽  
Solid Foundation

Hybridizations between domesticated escaped farmed fish and wild conspecifics may increase genetic diversity or break down locally adapted gene complexes, thus reducing competitive ability and overall fitness. We examined the genetic structure of six farmed populations of European seabass, Dicentrarchus labrax, of different geographical origin, and ten neighbouring wild populations in the Adriatic Sea using 10 microsatellite loci. All loci were polymorphic, with mean expected heterozygosity >74% in all 16 populations. Overall number of alleles as well as short-term effective population size were smaller in farmed (A = 18.7; Ne = 56.1) than in wild populations (A = 21.2; Ne = 180.6). The global FST of 0.0672 across loci showed significant population subdivision. Strong genetic differences between farmed fish and their wild conspecifics enabled the identification of seabass escapees back to their origins and the estimation of the extent of hybridization. Bayesian assignment analyses clustered wild populations together, whereas each farmed populations was assigned to a separate cluster. Intraspecific hybridization was highest in wild populations in areas impacted by fish farms, where highly admixed populations had decreased genetic diversity. The results of this study represent a solid foundation required to establish a genetic register of European seabass wild and farmed populations in the eastern Adriatic Sea that are required to establish a seabass DNA register.

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