Analysis of Toxoplasma gondii Antigens Recognized by Human Sera Obtained Before and After Acute Infection

1986 ◽  
Vol 154 (4) ◽  
pp. 650-657 ◽  
Author(s):  
I. Potasman ◽  
F. G. Araujo ◽  
G. Desmonts ◽  
J. S. Remington
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Before And After ◽  
Human Sera

scholarly journals Recombinant Antigens To Detect Toxoplasma gondii-Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay

2000 ◽  
Vol 38 (3) ◽  
pp. 1144-1150 ◽  
Author(s):  
D. Aubert ◽  
G. T. Maine ◽  
I. Villena ◽  
J. C. Hunt ◽  
L. Howard ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Acute Infection ◽  
Relative Sensitivity ◽  
Immunoglobulin M ◽  
Recombinant Antigens ◽  
Disease Spectrum ◽  
Human Sera ◽  
Acute Toxoplasmosis ◽  
Sensitivity Specificity

We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initial evaluation, six recombinant antigens (P29, P30, P35, P54, P66, and P68) were tested in the IgG and IgM Rec-ELISAs with four groups of samples which span the toxoplasmosis disease spectrum (negative, chronic infection, acute infection, and recent seroconversion). Our results suggest that the combination of P29, P30, and P35 in an IgG Rec-ELISA and the combination of P29, P35, and P66 in an IgM Rec-ELISA can replace the tachyzoite antigen in IgG and IgM serologic tests, respectively. The relative sensitivity, specificity, and agreement for the IgG P29-P30-P35 Rec-ELISA were 98.4, 95.7, and 97.2%, respectively. The resolved sensitivity, specificity, and agreement for the IgM P29-P35-P66 Rec-ELISA were 93.1, 95.0, and 94.5%, respectively. Relative to the tachyzoite-based immunocapture IgM assay, the IgM P29-P35-P66 Rec-ELISA detects fewer samples that contain IgG antibodies with elevated avidity from individuals with an acute toxoplasmosis.

scholarly journals Protein expression of the tear film of domestic cats before and after inoculation with Toxoplasma gondii

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Paula Elisa Brandão Guedes ◽  
Jéssica Fontes Veloso ◽  
Luciana Carvalho Lacerda ◽  
Juliano Oliveira Santana ◽  
Irma Yuliana Mora-Ocampo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Ocular Surface ◽  
Acute Infection ◽  
Tear Film ◽  
Chronic Phase ◽  
Surface Proteins ◽  
Domestic Cats ◽  
Significant Difference ◽  
Before And After ◽  
Peak Release

Abstract Background Tear film (TF) helps maintain and protect ocular function against damage to the ocular surface. Proteins are one of its main constituents, whose expression pattern can be used as a biomarker of ocular changes and systemic diseases. The aim of this study was to evaluate the expression of proteins in the TF of domestic cats before and after infection with Toxoplasma gondii, in the phases of acute infection and chronicity. Twelve healthy cats received orally homogenized brain matter obtained from mice inoculated with T. gondii oocysts, strain ME49. Cat feces were collected daily from the third day after infection to assess the release of oocysts. TF samples were obtained from cats, by Schirmer’s Tear Test 1, on day 0 (before infection), day 5 after infection (acute phase of infection, with maximum peak release of oocysts in feces) and on day 21 after infection (start of chronic phase, 7 days after total absence of oocyst release in feces). Tear samples were also submitted to proteomic analysis in a Q-Tof-Premier mass spectrometer. Results A total of 37 proteins with scores equal to or greater than 100 were identified on D0, followed by 36 on D5 and 42 on D21. Of these, 27 were common to D0 and D5, 33 to D0 and D21, 27 to D5 and D21, and 26 were common to the three groups, totaling 54 proteins. The most abundant proteins were lipocalin allergen Fel d, serum albumin, aldehyde dehydrogenase, lactoperoxidase and lactotransferrin. There was no significant difference in the abundance of proteins found on D0 and D5, but there was a statistical difference between D0 and D21 for ACT1_AEDAE, CERU_HUMAN and GELS_HUMAN. Regarding D5 and D21, there were significant differences for KV1_CANLF, LAC_PIG, TRFL_PIG, ACT1_AEDAE, CERU_HUMAN, GELS_HUMAN and OVOS2_HUMAN. Conclusions The main proteins identified in the TF of domestic cats are similar to those found in humans and other animal species. Most are part of the ocular surface defense system against injuries. The most expressed proteins in animals in the chronic phase of T. gondii infection are associated with the immune response to the parasite.

scholarly journals Seroprevalence of toxoplasmosis in Vojvodina

2010 ◽  
Vol 138 (5-6) ◽  
pp. 333-336 ◽  
Author(s):  
Snezana Brkic ◽  
Gorana Gajski ◽  
Mirjana Bogavac ◽  
Daniela Maric ◽  
Vesna Turkulov ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
General Population ◽  
Pregnant Women ◽  
Acute Infection ◽  
Clinical Manifestations ◽  
Congenital Toxoplasmosis ◽  
Reproductive Age ◽  
Female Population ◽  
Effective Measure ◽  
Female Patients

Introduction Toxoplasmosis is an acute infectious anthropozoonotic disease with mild asymptomatic clinical manifestations in immunocompetent persons and more severe in immunocompromised patients. Acute infection in pregnancy can result in severe congenital toxoplasmosis with severe sequels. Objective Aims of study were to detect Toxoplasma gondii seroprevalence in general population of Vojvodina, Serbia, differences between genders and determination of seroprevalence in women of reproductive age and pregnant women. Methods Our retrospective study was conducted from 2006 to 2008 including 625 immunocompetent patients, hospitalized or observed as outpatients at the Clinical Centre of Vojvodina, Novi Sad. We performed commercial ELISA kits SERION - ELISA classic test by VIRION for the presence of specific IgG and IgM antibodies. According to seroepidemiological aim of the study, our results were presented only in qualitative values. Results We observed 173 male and 452 female patients. Seroprevalence in general population of Vojvodina was 38.1%. In male population seroprevalence was 45.7%, and in female population it was 35.2%, the difference which was statistically significant (p<0.05). Seroprevalence increased with age and seroconversion was detected to occur in persons aged about 20 years. In all female patients, 353 (78.1%) were in reproductive age with seroprevalence of 30%. In 161 pregnant women seroprevalence was 31.7%. Conclusion In this study we screened actual seroepidemiological situation to Toxoplasma gondii in Vojvodina, thus giving a contribution to the continuous epidemiological screening done in this region and in the country. According to our results, almost 70% of women in reproductive age were sensitive to primary acute infection during further pregnancies, which is highly important for the prevention of congenital toxoplasmosis. Although not routinely conducted in many countries, routine serological testing to Toxoplasma gondii in pregnant women and their education about preventive measures against this infection could be an effective measure in the future.

Acute infection with an avirulent strain of Toxoplasma gondii causes decreasing and atrophy of nitrergic myenteric neurons of rats

2017 ◽  
Vol 119 (4) ◽  
pp. 423-427 ◽  
Author(s):  
Débora de Mello Gonçales Sant’Ana ◽  
Marcelo Biondaro Gois ◽  
Catchia Hermes-Uliana ◽  
Letícia Sarturi Pereira-Severi ◽  
Emily Martins Baptista ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Avirulent Strain ◽  
Myenteric Neurons

scholarly journals Recombinant MAG1 Protein of Toxoplasma gondii as a Diagnostic Antigen

2015 ◽  
Vol 64 (1) ◽  
pp. 55-59 ◽  
Author(s):  
JUSTYNA M. GATKOWSKA ◽  
BOŻENA DZIADEK ◽  
JAROSŁAW DZIADEK ◽  
KATARZYNA DZITKO ◽  
HENRYKA DŁUGOŃSKA
Keyword(s):  
Toxoplasma Gondii ◽  
Full Length ◽  
Specific Marker ◽  
Igg Antibodies ◽  
Diagnostic Antigen ◽  
Human Sera ◽  
Diagnostic Usefulness ◽  
Native Antigen

The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii infection since the protein was recognized by both mouse and human sera, 100% and 94.3%, respectively, rendering the full-length rMAG1 a prospective alternative for the polyvalent native antigen (TLA).

scholarly journals Transcriptome Analysis of Testes and Uterus: Reproductive Dysfunction Induced by Toxoplasma gondii in Mice

2020 ◽  
Vol 8 (8) ◽  
pp. 1136
Author(s):  
Junjie Wang ◽  
Tanghui Liu ◽  
Yasser S. Mahmmod ◽  
Zipeng Yang ◽  
Jiexing Tan ◽  
...  
Keyword(s):  
Gene Ontology ◽  
Toxoplasma Gondii ◽  
Acute Infection ◽  
The Other ◽  
Rna Seq ◽  
Mice Model ◽  
Reproductive Disorders ◽  
Kegg Database ◽  
Disease Pathogenesis ◽  
Differently Expressed Genes

Toxoplasma gondii (T. gondii) infection in female mammals during pregnancy can result in poor pregnancy. Similarly, it can result in male reproductive disorders in male mammals. Although the testes and uterus have very different biological makeup, they are still both attacked by T. gondii resulting in reproductive dysfunctions. We hypothesized that there are significant common genes in the testes and uterus that interact with T. gondii. Finding out and studying these genes is vital to understand the infection mechanism of T. gondii and the induced disease pathogenesis. To achieve this goal, we built a mice model of acute infection with T. gondii and the testes and uterus of the mice were sequenced by RNA-Seq. A total of 291 and 679 significantly differently expressed genes (DEGs) were obtained from the testes and the uterus, respectively. In the Gene Ontology (GO) analysis, part of the DEGs in the testes and uterus were related to 35 GO functions. When compared with the KEGG database, seven pathways affecting both the testes and uterus during the course of T. gondii infection were identified. In addition, Toxoplasmosis can significantly affect the expression of Nlrp5 and Insc leading to negative outcomes in the host. On the other hand, the host regulates Gbp7, Gbp2b, and Ifit3 to defend against T. gondii infection.

scholarly journals Detection of Anti-Toxoplasma gondii Antibodies in Human Sera Using Synthetic Glycosylphosphatidylinositol Glycans on a Bead-Based Multiplex Assay

2019 ◽  
Vol 91 (17) ◽  
pp. 11215-11222 ◽  
Author(s):  
Monika Garg ◽  
Daniel Stern ◽  
Uwe Groß ◽  
Peter H. Seeberger ◽  
Frank Seeber ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Multiplex Assay ◽  
Human Sera

scholarly journals Evolution of IgG antibody response against Toxoplasma gondii tissue cyst in acute and chronic human infections

1998 ◽  
Vol 40 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Margarita VILLAVEDRA ◽  
Hernán CAROL ◽  
Alberto NIETO
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Chronic Phase ◽  
Tissue Cyst ◽  
Specific Response ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Wide Range ◽  
Toxoplasmic Infection ◽  
Human Infections

The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected patients at acute and chronic phase. There was an overall coincidence of molecular mass among antigens of tachyzoites and tissue cysts recognized by these sera, however, they appear not to be the same molecules. The response against tissue cysts starts early during acute infection, and the reactivity of antibodies is strong against a wide range of antigens. Six bands (between 82 and 151 kDa) were exclusively recognized by chronic phase sera but only the 132 kDa band was positive in more than 50% of the sera analysed. A mixture of these antigens could be used to discriminate between the two infection phases. The most important antigens recognized by the acute and the chronic phase sera were 4 clusters in the ranges 20-24 kDa, 34-39 kDa, 58-80 kDa and 105-130 kDa as well as two additional antigens of 18 and 29 kDa. Both accidentally infected patients and some of the naturally infected patients showed a weak specific response against tissue cyst antigens.

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