Method for Isolation and Detection of Dioctyl Phthalate from Milk Lipids

1967 ◽  
Vol 50 (3) ◽  
pp. 646-650
Author(s):  
J Cerbulis ◽  
J S Ard
Keyword(s):  
Fatty Acids ◽  
Infrared Spectroscopy ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Dairy Farm ◽  
Dioctyl Phthalate ◽  
Ether Extract ◽  
Alumina Column ◽  
Milk Lipids ◽  
Layer Chromatography

Abstract A method has been developed to isolate dioctyl phthalate (DOP, di-(2-ethyIhexyI) phthalate) from milk. Milk from an individual dairy farm was dialyzed and evaporated to dryness, and the dry residue was extracted with petroleum ether. The petroleum ether extract of dried milk was chromatographed on an alumina column as described by Hanahan. The fraction containing free fatty acids and DOP was separated by thin layer chromatography (TLC). DOP was distinguished from other phthalates by TLC and infrared spectroscopy

scholarly journals Insecticidal activity of different fractions of petroleum ether extract of Zingiber cassumunar rhizome against Tribolium castaneum

2015 ◽  
Vol 50 (2) ◽  
pp. 143-152 ◽  
Author(s):  
D Talukder ◽  
AB MH Haque ◽  
S Zaman ◽  
MS Islam ◽  
MN Islam ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Petroleum Ether Extract ◽  
Preparative Thin Layer Chromatography ◽  
Contact Toxicity ◽  
Ether Extract ◽  
Small Column ◽  
Moderate Effect ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

An experiment was carried out to investigate the efficacy of contact toxicity of different fractions of petroleum ether extract of Zinziber cassumunar Roxb. rhizome against Tribolium castaneum. Seventy-eight different fractions of petroleum ether extract were obtained from column chromatography. Elutes having the similar TLC behavior were combined in eight fractions and were named as: MN-1, MN-2, MN-3, MN-4, MN-5, MN-6, MN-7 and MN-8. Four separate fractions were collected from the MN-1 fraction by preparative thin layer chromatography. These four parts were washed with petroleum ether, chloroform, ethyl acetate and methanol. Thereafter, these were separated by small column and designated as: M1N1, M1N2, M1N3, M1N4, M2N1, M2N2, M2N3, M2N4, M3N1, M3N2, M3N3, M3N4, M4N1 and M4N2. Fractions of M1N2, M2N2, M3N2 and M4N2 were found to be the most effective against the beetle T. castaneum after 24, 48 and 72 hours. However, some fractions exhibited the moderate effect and other fractions did not work against the beetle.Bangladesh J. Sci. Ind. Res. 50(2), 143-152, 2015

CHEMICAL INVESTIGATION OF THE CORMS OF ARISAEMA TRIPHYLLUM (L.) SCHOTT

1934 ◽  
Vol 10 (2) ◽  
pp. 164-169
Author(s):  
Léo Marion
Keyword(s):  
Fatty Acids ◽  
Essential Oil ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Water Soluble ◽  
Chemical Investigation ◽  
Crystalline Substance ◽  
Ether Extract ◽  
Arisaema Triphyllum

The dried and ground corms lost 5% of their weight when extracted with petroleum ether, and a further 0.9% on subsequent extraction with methanol. Besides a trace of an essential oil, the methanol extract yielded water-soluble and water-insoluble portions. The former contained an acid, C5H8O5, m.p. 184 °C.; i-inositol, C6H6(OH)6, m.p. 224 °C.; a sugar forming phenylglucosazone; and a crystalline substance, (C6H10O6 or C7H12O7), m.p. 120 °C., exhibiting the properties of a lactone. In the water-insoluble portion combined with the petroleum ether extract, the following substances have been found present: myricyl alcohol, C30H62O, m.p. 82–83 °C.; a new sterol, arisaesterol, C36H64O2, m.p. 135 °C.; phytosterolin, C31H52O6, m.p. 297 °C.; and a mixture of fatty acids.

scholarly journals Proximate Composition, Physicochemical Properties and Antioxidant Activity of Flaxseed

2020 ◽  
pp. 1-10
Author(s):  
Omer Adam Omer Ishag ◽  
Ayat A. Khalid ◽  
Amina Abdi ◽  
Ibrahim Yaagoub Erwa ◽  
Awadalla Babiker Omer ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Physicochemical Properties ◽  
Petroleum Ether ◽  
Proximate Composition ◽  
Petroleum Ether Extract ◽  
Flaxseed Oil ◽  
Ether Extract

Aims: The aims of this study were to investigate the proximate composition of flaxseed; physiochemical properties and antioxidant activity of flaxseed. Study Design: Using standard analytical methods to determine the proximate composition of flaxseed, extraction of flaxseed in different trials under the same conditions using two different solvents and investigating their physicochemical properties and antioxidant activity. Place and Duration of Study: This study was conducted in the laboratory of department of applied and industrial chemistry, International University of Africa, University of Medical Sciences and Technology and National Centre for Research, Khartoum – Sudan, during June 2019. Methodology: The prepared sample of flaxseeds was tested for proximate composition and then extracted with petroleum ether and n-hexane. The extracted oil was then subjected to physicochemical analysis, GC-MS analysis and antioxidant activity test. Results: The oil yield of flaxseed was high 21.95% and 28.29% with n-hexane and petroleum ether respectively. The results obtained for proximate composition were: moisture (8.50 ± 0.49%), ash (1.96 ± 0.00%), fiber (20.23 ± 3.47%), protein (21.00 ± 0.74%),fat (43.17 ± 0.99%) and carbohydrate (5.14 ± 2.73%).The physicochemical properties of n-hexane and petroleum ether extracted flaxseed oils were: density (0.92 ± 0.00 g/cm3), refractive index (1.47 ± 0.00 and 1.48 ± 0.01 at 28°C), acid value (0.76 ± 0.10 and 0.75 ± 0.20 mg KOH/g), peroxide value (4.67 ± 1.00 and 2.69 ± 0.55 meq O2/kg), saponification value (185.61 ± 0.56 and 187.83 ± 2.08 mg KOH/g oil), unsaponifiable matter (5.57 ± 0.64 and 2.83 ± 0.23 mg KOH/g oil) and iodine value (97.24 ± 0.86 and 97.44 ± 2.93 g/100 g) respectively. Statistical analysis showed no significant difference between the means of the two data sets of physicochemical properties of flaxseed oils extracted with the two solvents used at 95% confidence level. The GC-MS spectrum of flaxseed oil extracted with n-Hexane revealed presence of 27 fatty acids, the predominant were linoleic acid (46.53%), palmitic acid (18.04%), stearic acid (12.23%), linolenic acid (11.63%) and oleic acid (4.24%). While for petroleum ether extract a total of 28 fatty acids were identified the major were: linolenic acid (42.39%), linoleic acid (26.17%), palmitic acid (12.89%), stearic acid (10.73%) and  methyl erucic (1.24%.). The oils also, showed good antioxidant activity in relation to control; 28 ± 0.09 (DPPH) for n-Hexane extract and 26 ± 0.05 (DPPH) for petroleum ether extract. Conclusion: Based on the results obtained flaxseed and flaxseed oil had good potential for human consumption and can be used for other application.

scholarly journals Analysis on the Fatty Acids and Volatile Components in Pleurotus geesteranus by HS-SPME-GC-MS

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Zhenhua Liang ◽  
Shanei Li ◽  
Qiongxin Liang ◽  
Liqiang Ji ◽  
Jinmei Wang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Petroleum Ether ◽  
Solid Phase ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Ethyl Linoleate ◽  
Ion Current ◽  
Volatile Components ◽  
Ether Extract ◽  
Ethyl Palmitate

The volatile constituents and fatty acids in Pleurotus geesteranus were assayed by headspace solid-phase microextraction coupled with GC-MS. There were 5 volatile compounds in P. geesteranus that accounted for 43.43% of the total ion current peak area, and its main compounds were 2-undecanone (13.99%), 3-ethyl-2,5-dimethyl pyrazine (12.67%), and l-β-bisabolene (6.79%). Fourteen compounds were identified in the ethanol extract of P. geesteranus and 6 fatty acids were identified from the petroleum ether extract, which accounted for 93.72% and 98.48% of the total ion current peak area, the main compounds in the ethanol extract were ethyl linoleate (67.36%) and ethyl palmitate (21.83%), and the main fatty acids in the petroleum ether extract were linoleic acid (78.22%), palmitic acid (10.74%), and oleic acid (8.13%).

Antioxidant lignans from petroleum ether extract of Schisandra chinensis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
J Slanina ◽  
L Bøezinová ◽  
H Paulová ◽  
O Humpa
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Schisandra Chinensis ◽  
Ether Extract

The struggle with rheumatism through Dracunculus vulgaris Schott: In the light of ethnobotanical information.

2020 ◽  
Vol 13 ◽  
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Mert Ilhan ◽  
Esra Küpeli Akkol
Keyword(s):  
Petroleum Ether ◽  
Inflammatory Diseases ◽  
Petroleum Ether Extract ◽  
Major Constituent ◽  
Control Group ◽  
Ether Extract ◽  
Test Models ◽  
Mouse Ear ◽  
Gas Chromatography Mass Spectroscopy ◽  
Abdominal Constriction

Background: The fruits with the seeds of Dracunculus vulgaris Schott. (Araceae) are used against inflammatory diseases in Turkey. Objective: Present study was designed to justify this folkloric usage type of the plant. Therefore, the aim of this study is to investigate the anti-inflammatory activity of D. vulgaris. Methods: Petroleum ether, ethyl acetate and methanol extracts were prepared from the fruits, successively. Carrageenan-, serotonin-, and prostaglandin E2-induced hind paw edema; acetic acid–induced capillary permeability and 12-O-tetradecanoyl-phorbol-13-acetate–induced mouse ear edema models were used to assess the antiinflammatory activity of the extracts. The analgesic activity was experienced by using p-benzoquinone-induced abdominal constriction test. Results: The petroleum ether extract displayed the highest activities in all of the used test models compared with the control group. Therefore, the constituents of this extract were determined by using gas chromatography–mass spectroscopy (GC–MS). Linoleic acid was found to be major constituent of the petroleum ether extract of D. vulgaris. Conclusion: This study has provided some justification for the folkloric use of the plant.

scholarly journals The petroleum ether extraction of Brassica Rapa L. induces apoptosis of Lung adenocarcinoma cells via mitochondria-dependent pathway

2021 ◽  
Author(s):  
Xierenguli Halike ◽  
Jin-Yu Li ◽  
Pengfei Yuan ◽  
Kaimeiliya Yasheng ◽  
Min Chen ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Brassica Rapa ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Traditional Food ◽  
Ether Extract ◽  
Ether Extraction ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
Dependent Pathway

Brassica rapa L. is one of the most popular traditional food with a variety of biological activities. In this study, the petroleum ether extract of B. rapa was separated by...

Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

2021 ◽  
Vol 20 ◽  
Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Petroleum Ether Extract ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
Membrane Stabilization ◽  
Leaf Extracts ◽  
Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

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