Emulsifiers. III. Periodic Acid Analysis for 1-Monoglycerides

Abstract Two collaborative studies were conducted to compare the official AOCS method, Cd 11–57, and a revised Miner procedure. The second study used known materials to obtain accuracy information. Studies showed that the revised Miner procedure is more accurate and more precise at low monoglyceride levels such as those encountered in commercial shortenings. It is recommended that the Miner procedure be adopted as official first action. It is further recommended that the joint AOAC-AOCS methods study of these two procedures be continued so that a single common method can be made official by both societies.

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Emulsifiers. II. Periodic Acid Analysis for 1-Monoglycerides

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/49.4.816 ◽

1966 ◽

Vol 49 (4) ◽

pp. 816-820

Author(s):

F J Baur ◽

E Distler

Keyword(s):

Analytical Methods ◽

Periodic Acid ◽

Aocs Method ◽

Official Aocs Method ◽

Official Aocs

Abstract Two periodic acid analytical methods have been compared for the determination of 1-monoglycerides. A revised Miner procedure is apparently faster and more precise than the official AOCS method, Cd 11-57. The AOCS procedure is recommended for adoption as official, first action. A joint AOAC-AOCS multi-methods study should be conducted to compare the Miner and AOCS procedures.

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Accuracy of Peanut Moisture Determinations by Using the Official AOCS Method1

Peanut Science ◽

10.3146/i0095-3679-2-1-8 ◽

1975 ◽

Vol 2 (1) ◽

pp. 29-32

Author(s):

G. H. Brusewitz ◽

T. B. Whitaker ◽

J. H. Young

Keyword(s):

North Carolina ◽

Standard Deviation ◽

Collaborative Study ◽

Moisture Determination ◽

State University ◽

Aocs Method ◽

Oklahoma State University ◽

North Carolina State University ◽

The Mean ◽

Official Aocs

Abstract A collaborative study was conducted using Spanish peanuts at Oklahoma State University and Virginia peanuts at North Carolina State University regarding the variability in moisture determination using the official AOCS method. The variability among 30 samples was estimated at 5 moisture levels for each of the two peanut types. No difference could be detected between the two types and the data were combined. The standard deviation was linearly related to the mean moisture. Data provided information necessary to compute the number of samples required for a desired precision.

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Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080626 ◽

1977 ◽

Vol 35 ◽

pp. 640-641

Author(s):

J. R. Ruby

Keyword(s):

Endoplasmic Reticulum ◽

Parotid Gland ◽

Periodic Acid ◽

Acinar Cells ◽

Duct System ◽

Parotid Glands ◽

Dasypus Novemcinctus ◽

Anterior Portion ◽

Periodic Acid Schiff ◽

Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

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Demonstration of Retinal Glycogen with Silver Methenamine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066875 ◽

1971 ◽

Vol 29 ◽

pp. 564-565

Author(s):

A. W. Sedar ◽

G. H. Bresnick

Keyword(s):

Lactic Acid ◽

Chromic Acid ◽

Periodic Acid ◽

Müller Cell ◽

External Limiting Membrane ◽

Electron Microscope Level ◽

Reactive Process ◽

Inner Limiting Membrane ◽

Muller Cell ◽

And Migration

After experimetnal damage to the retina with a variety of procedures Müller cell hypertrophy and migration occurs. According to Kuwabara and others the reactive process in these injuries is evidenced by a marked increase in amount of glycogen in the Müller cells. These cells were considered originally supporting elements with fiber processes extending throughout the retina from inner limiting membrane to external limiting membrane, but are known now to have high lactic acid dehydrogenase activity and the ability to synthesize glycogen. Since the periodic acid-chromic acid-silver methenamine technique was shown to demonstrate glycogen at the electron microscope level, it was selected to react with glycogen in the fine processes of the Müller cell that ramify among the neural elements in various layers of the retina and demarcate these cells cytologically. The Rhesus monkey was chosen as an example of a well vascularized retina and the rabbit as an example of a avascular retina to explore the possibilities of the technique.

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Cytochemical Localization of Polysaccharides in Diplodia Maydis With Thiosemicarbazide : Evaluation of Three Fixatives For Pa:Tsc:Os Reaction

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090221 ◽

1976 ◽

Vol 34 ◽

pp. 48-49 ◽

Cited By ~ 1

Author(s):

Judith A. Murphy ◽

Mary R. Thompson ◽

A.J. Pappelis

Keyword(s):

Reaction Center ◽

Osmium Tetroxide ◽

Periodic Acid ◽

Cytochemical Localization ◽

Thin Sections ◽

Selective Reaction ◽

Amorphous Character

In an attempt to identify polysaccharide components in thin sections of D. maydis, procedures were employed such that a PAS localization could be carried out. Three different fixatives were evaluated ie. glutaraldehyde, formaldehyde and paraformaldehyde. These were used in conjunction with periodic acid (PA), thiosemicarbazide(TSC), and osmium tetroxide(Os) to localize polysaccharides in V. maydis using a pre-embedded reaction procedure. Polysaccharide localization is based on the oxidation of vic-glycol groups by PA, and the binding of TSC as a selective reaction center for the formation of osmium black. The reaction product is sufficiently electron opaque, insoluble in lipids, not altered when tissue is embedded, and has a fine amorphous character.

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Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130109 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1094-1095

Author(s):

Beverly L. Giammara ◽

Jennifer S. Stevenson ◽

Peggy E. Yates ◽

Robert H. Gunderson ◽

Jacob S. Hanker

Keyword(s):

Image Analysis ◽

Basement Membrane ◽

Periodic Acid ◽

Light And Electron Microscopy ◽

Computer Assisted ◽

Type Iii Collagen ◽

Image Analysis System ◽

Biodegradable Polyester ◽

Adult Rats ◽

Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

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Rheometers, Bioreactors, and Vocalization Forces: Using Basic Science Investigations to Help the Voices of Teachers

Perspectives on Voice and Voice Disorders ◽

10.1044/vvd18.3.119 ◽

2008 ◽

Vol 18 (3) ◽

pp. 119-125

Author(s):

Sarah Klemuk

Keyword(s):

Basic Science ◽

Tissue Growth ◽

University Of Iowa ◽

Support Cell ◽

Rest Periods ◽

Collaborative Studies ◽

Professional Voice Users ◽

The University ◽

Science Investigations ◽

Professional Voice

Abstract Collaborative studies at the University of Iowa and the National Center for Voice and Speech aim to help the voices of teachers. Investigators study how cells and tissues respond to vibration doses simulating typical vocalization patterns of teachers. A commercially manufactured instrument is uniquely modified to support cell and tissue growth, to subject tissues to vocalization-like forces, and to measure viscoelastic properties of tissues. Through this basic science approach, steps toward safety limits for vocalization and habilitating rest periods for professional voice users will be achieved.

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The Role of Positive and Negative Affectivity in Healthy and Unhealthy Work-Related Behavior and Experiences

European Journal of Health Psychology ◽

10.1027/2512-8442/a000030 ◽

2019 ◽

Vol 26 (2) ◽

pp. 56-67 ◽

Cited By ~ 2

Author(s):

Hanna Hofmann ◽

Carl-Walter Kohlmann

Keyword(s):

Negative Affectivity ◽

Coping Behavior ◽

Common Method ◽

Positive Affectivity ◽

Work Related ◽

Teacher Students ◽

And Behaviors ◽

Main Effects ◽

Positive And Negative Affectivity

Abstract. Positive affectivity (PA) and negative affectivity (NA) are basic traits that affect work-related perceptions and behaviors and should be considered in any assessment of these variables. A quite common method to assess healthy or unhealthy types of work-related perceptions and behaviors is the questionnaire on Work-Related Coping Behavior and Experience Patterns (WCEP). However, the association of PA and NA with WCEP remained unclear. In a sample of teachers, physiotherapists, and teacher students ( N = 745; Mage = 35.07, SD = 12.49; 78% females), we aimed to identify the relevance of these basic traits. After controlling for age, gender, and type of occupation, we found main effects of PA and NA, with the specific combination of PA and NA being decisive for predicting the assignment to a WCEP type. The results highlight the need to include PA and NA in future assessments with the WCEP questionnaire.

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