A Convenient Thin Layer Chromatographic Cleanup Procedure for Screening Several Mycotoxins in Oils

1975 ◽  
Vol 58 (3) ◽  
pp. 620-621
Author(s):  
Susie N Hagan ◽  
William H Tietjen
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Formic Acid ◽  
Soybean Oil ◽  
Ethyl Acetate ◽  
Aflatoxin B1 ◽  
Corn Oil ◽  
Second Development ◽  
Cleanup Procedure ◽  
Seed Extracts

Abstract A thin layer chromatographic cleanup development with benzene-hexane (34+1) effectively removed lipids and some contaminants from mixtures of mycotoxins in corn oil, olive oil, peanut oil, soybean oil, and seed extracts. A second development in the same direction as the first, using toluene-ethyl acetate-formic acid ( 6+3+1 ) or benzene-acetic acid (9+l), separated the mycotoxins. Satisfactory separation was achieved for commercial oils spiked with sterigmatocystin, zearalenone, ochratoxins A, B, and C, and anatoxins B1 B2, Gl and G2. This technique permits detection of 5 ppb aflatoxin B1 in corn.

Confirmatory Tests for Aflatoxin B1

1964 ◽  
Vol 47 (5) ◽  
pp. 801-803 ◽  
Author(s):  
Peter John Andrellos ◽  
George R Reid
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Formic Acid ◽  
Thionyl Chloride ◽  
Aflatoxin B1 ◽  
Trifluoroacetic Acid ◽  
Reaction Products ◽  
Confirmatory Tests ◽  
Aflatoxin B ◽  
Layer Chromatography

Abstract Three confirmatory tests have been devised to identify aflatoxin B±. Portions of the isolated toxin are treated with formic acid-thionyl chloride, acetic acid-thionyl chloride, and trifluoroacetic acid, respectively, and aliquots of the three fluorescent reaction products are spotted on thin-layer chromatography plates. Standards treated with each of the three reagents, plus an untreated standard, are spotted on the same plate, and after development the spots are compared under ultraviolet light.

Thin Layer Chromatographic Determination of Aflatoxin in Corn Dust

1981 ◽  
Vol 64 (5) ◽  
pp. 1060-1063 ◽  
Author(s):  
Odette L Shotwell ◽  
William R Burg ◽  
Thomas Diller
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Aflatoxin B1 ◽  
American Association ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Acetone Water ◽  
Layer Chromatography

Abstract Methods adopted by the AOAC and the American Association of Cereal Chemists for determining aflatoxin in corn were modified, and techniques were developed for application to samples of <1 to 10 g instead of the specified 50 g samples. Analysis included chloroform extraction of dust samples or dust collected from glass fiber filters, purification of extracts on a silica gel column of appropriate size, and measurement of aflatoxin by either 1- or 2-dimensional thin layer chromatography (TLC). The solvent for 1-dimensional TLC was chloroform-acetonewater (91 + 9 + 1). Solvents for 2-dimensional TLC were, first direction, ether-methanol-water (95 + 4 + 1, lined tank) and second direction, chloroformacetone- water (91 + 9 + 1, unlined tank), or first direction, chloroform-acetone-water (91 + 9 + 1, unlined tank) and second direction, toluene-ethyl acetate- formic acid (60 + 30 + 10, unlined tank). When samples weighed ≤0.1 g, the entire concentrated extract was applied to the TLC plate. About 0.5-1.0 ng aflatoxin B1 could be detected on the plate, making the limit of detection about 9 ng/g for 0.1 g samples.

scholarly journals Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Sharada L. Deore ◽  
Payal S. Jaju ◽  
Bhushan A. Baviskar
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
High Performance ◽  
Glacial Acetic Acid ◽  
Simultaneous Estimation ◽  
Retention Factors ◽  
Ich Guidelines ◽  
Cough Syrup ◽  
Hptlc Method

A new simple, rapid, selective and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous estimation of vasicine, glycyrrhizin, eugenol, and cineole in herbal cough syrup. The retention factors of vasicine, glycyrrhizin, eugenol, and cineole are 0.53, 0.44, 0.75, and 0.77, respectively. Chromatography was performed on 60F254 percolated TLC plate using n-hexane : ethyl acetate : glacial acetic acid (8.5 : 1.0 : 0.5 v/v/v). Methods are validated according to ICH guidelines and can be adopted for the routine analysis of vasicine, glycyrrhizin, eugenol and cineole in herbal cough syrup.

scholarly journals Analytical profiling and structure elucidation of flavanoids compounds

2019 ◽  
pp. 7-12
Author(s):  
Rajni Singh Khureja ◽  
Dinesh Kumar
Keyword(s):  
Acetic Acid ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Structure Elucidation ◽  
Uv Light ◽  
Methyl Ethyl ◽  
Aerial Parts ◽  
Acetate Methyl ◽  
Layer Chromatography

Chromatography is a powerful analytical method suitable for the separation and quantitative determination of a considerable number of compounds, even from complicated matrix. Thin Layer Chromatography (TLC) has some advantages such as rapidity, sensitivity, easiness, cheapness and this method does not require complex instrumental equipment. In present study, Quercetin 3 -O -α- d- glucuro pyranoside (miquelianin; QG), quercetin 3-O-α-d glucopyranoside (isoquercitrin), quercetin 3-O-α-d-galactopyranoside (hyperoside) and rutin in ethyl acetate fractions from aerial parts of selected Potentilla species on a HPTLC plates using a mixture consisting of ethyl acetate/methyl ethyl ketone/diisopropyl ether/formic acid (3:10:4:1, v/v/v/v). Rutinosides and quercetin were also eluted using methanol-water-acetic acid (50/44/6, v/v/v) and benzene: pyridine: formic acid (36:9:5). The spot visualization was evaluated under UV light at 254 nm and Ferric chloride reagent.

Isolation and Identification of Hyperoside in Abelmoschus manihot Flower

2013 ◽  
Vol 781-784 ◽  
pp. 880-883
Author(s):  
Yan Qiu ◽  
Ming Zhu Wang
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Isolation And Identification ◽  
Ethanol Extraction ◽  
Light Yellow ◽  
Yellow Stripe ◽  
Layer Chromatography ◽  
Chloroform Methanol

Abelmoschus Manihot (L.) Medic flowers are traditionally used for food and medicinal materials. With the aim of acquiring the best method of isolating hyperoside from total flavone in A. Manihot flowers, this study investigated the effects of different eluted factors on separating hyperoside in total flavone of crude extracts. Gradient elution was used to separate the total flavone of ethanol extraction. The eluates were isolated by silica gel thin-layer chromatography (TLC) and identified by HPLC. The results showed that the elution components (chloroform: methanol = 50: 50) isolated by TLC (developing agent ethyl acetate: formic acid: water = 8:3:1) presented a clear light yellow stripe which was identified as hyperoside by HPLC.

scholarly journals Determination of saccharin in pharmaceuticals by high performance thin layer chromatography

2006 ◽  
Vol 71 (6) ◽  
pp. 669-676 ◽  
Author(s):  
Mira Cakar ◽  
Gordana Popovic
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Sodium Salt ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Pharmaceutical Preparations ◽  
Linear Calibration ◽  
Saccharin Sodium

A simple, accurate and selective high performance thin layer chromatographic method for the determination of saccharin in pharmaceuticals has been developed. The chromatography was performed on silica-gel 60F254 plates with ethyl acetate-carbon tetrachloride-acetic acid (3 + 4 + 0.5 v/v/v) as the mobile phase. The chromatographic zones corresponding to the saccharin spots were scanned in the reflectance/absorbance mode at ?=230 mm. For the standard curves, two series of saccharin sodium salt solutions were prepared: in methanol (solvent 1) and in ethyl acetate-acetic acid (9:1, v/v) mixture (solvent 2). A linear calibration relationship was observed within the concentration range from 300-1200 ng saccharin sodium salt per spot correlation coefficients being 0.998 (solvent 1) and 0.995 (solvent 2). The relationship between the peak area and the amount of saccharin sodium salt was evaluated by linear regression analysis. The limits of detection and quantification of saccharin sodium salt were 35 ng and 110 ng per spot (solvent 1), respectively, and 45 ng and 150 ng per spot (solvent 2) respectively. Mean recovery values of 103.5 % (solvent 1) and 102.3 % (solvent 2), and RSD values of 4.42 % (solvent 1) and 2.53 % (solvent 2) were obtained. The proposed method was applied for saccharin determination in two pharmaceutical preparations, effervescent tablets and a carbomer- based gel.

scholarly journals Determination of Rosmarinic Acid and Caffeic Acid in Thymus daenensis and Thymus lancifolius Extracts by High-Performance Thin Layer Chromatography

2021 ◽  
Vol 10 (4) ◽  
pp. 2804-2809
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Layer Chromatography

Thymus species belong to the Lamiaceae family, of which 18 species in the flora of Iran, 6 are endemic to Iran. In the current research, high-performance thin-layer chromatography (HPTLC) technique as an easy, fast, reproducible, and low-cost method was used for the determination of rosmarinic acid and caffeic acid in Thymus lancifolius (T. lancifolius) and two species of Thymus daenensis (T. daenensis) from Iran. Toluene-ethyl acetate-formic acid with a ratio of 67.72-22.90 and 9.38% was selected as the mobile phase of rosmarinic acid, and ethyl acetate-methanol-formic acid-water with a ratio of 85-8-2 and 5% was designated as the mobile phase of caffeic acid. The highest and lowest amount of rosmarinic acid was observed for T. daenensis 1 (10.54±0.12 mg/g) and T. lancifolius (0.46±0.01 mg/g), respectively. The amount of rosmarinic acid for T. daenensis 2 was obtained as 7.85±0.02 mg/g for each of the dried plants. In the following, HPTLC analysis of caffeic acid for T. daenensis 1, T. daenensis 2, and T. lancifolius was acquired amounts of 0.78±0.007, 0.13±0.007, and 0.26±0.007 mg/g for each of dried plants, respectively. Therefore, regarding the special effects of phenolic acids and properties of the Thymus genus, the acquired results are suitable for application in pathogenic research, infections, immunology diseases, and evaluation of the antioxidant activity.

scholarly journals CHROMATOGRAPHIC STUDIES ON BIOACTIVE COMPOUNDS OF ETHANOLIC LEAF EXTRACT OF AERVA LANATA BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY TECHNIQUE

2017 ◽  
Vol 10 (6) ◽  
pp. 340
Author(s):  
Lucia Sounder ◽  
Victor Arockia Doss
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
High Performance ◽  
Ethanolic Extract ◽  
Different Types ◽  
Rf Values ◽  
Aerva Lanata ◽  
Layer Chromatography

 Objective: This study was designed to determine the bioactive compounds such as alkaloids, glycosides, phenol, and tannins by high performance thin layer chromatography (HPTLC) which will help in crude drug identification and in the standardization of Aerva lanata in pharmacological industries. Methods: HPTLC studies were conducted as Harborne described. The toluene-acetone-formic acid (4.5:4.5:1); ethyl acetate-ethanol-water (10:1.35:1); ethyl acetate-ethanol-water (8:2:1.2); toluene-ethyl acetate-formic acid-methanol (3:3:0.8:0.2) were employed as mobile phase for phenol, alkaloid, glycoside, and tannin profiles. Result: The ethanolic extract of leaves of A. lanata illustrated the presence of 11 different types of phenol with 11 different Rf values with range 0.06- 0.95, 10 different types of alkaloid with 10 different Rf values from 0.02 to 0.92, 12 different types of glycoside with 12 different Rf values from 0.02 to 0.96, 9 different types of Tannin with 9 different Rf values from 0.07 to 0.93. Conclusion: This study supplements valuable information about known and unknown bioactive compounds with the bioactivity of A. lanata. Further, pharmacological studies on structure of the bioactive compounds can be formulated to treat diseases. Thus, the ethanolic extract of A. lanata plant can be utilized as a useful medicinal herb for alleviation of various illness and disorder. 

Determination of Bergapten in Fragrance Preparations by Thin Layer Chromatography and Spectrophotofluorometry

1976 ◽  
Vol 59 (3) ◽  
pp. 547-551
Author(s):  
Harris H Wisneski
Keyword(s):  
Acetic Acid ◽  
Carbon Tetrachloride ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Digital Integrator ◽  
Average Recovery ◽  
Tert Butylamine ◽  
Layer Chromatography

Abstract A method has been developed for the determination of bergapten (5-methoxypsoralen), a known phototoxin, in perfumes, colognes, and toilet waters. The bergapten and other lactonic compounds were first isolated from the sample by a series of extractions. The extract containing the bergapten was diluted to a known volume and an aliquot was spotted on a thin layer chromatographic (TLC) plate coated with silica gel G. After 2-dimensional development with hexane - carbon tetrachloride - tert - butylamine (180 + 12 + 9) and hexane-toluene-ethyl acetate-acetic acid (100+10+15+0.5), the TLC plate was dried and the emitted fluorescence of bergapten was measured, using a spectrophotofluorometer equipped with a TLC accessory and coupled to an automatic digital integrator. The amount of bergapten was determined by comparing its peak area to those of bergapten standards. The average recovery for levels of 0.001, 0.005, and 0.01% bergapten was 88%.

Sign in / Sign up

Export Citation Format

Share Document