Extraction, Cleanup, and Quantitative Determination of Aflatoxins B1 and M1 in Beef Liver

1979 ◽  
Vol 62 (5) ◽  
pp. 1080-1082
Author(s):  
Mary W Trucksess ◽  
Leonard Stoloff
Keyword(s):  
Citric Acid ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Animal Tissues ◽  
Beef Liver ◽  
Layer Chromatography ◽  
Extracting Solvent

Abstract A method for the determination of aflatoxin Bx in eggs was applicable for aflatoxin B1 in liver, but ineffective for aflatoxin Mx in liver because of poor recovery of added aflatoxin and interferences in thin layer chromatography. The method was modified by the addition of citric acid to the extracting solvent and ammonium sulfate to the extract solution for removing protein. The elution system for silica gel column cleanup was also changed by substituting methanol for acetone, and adding a step for confirmation of aflatoxin M1 identity. The method has been used successfully for survey and research on aflatoxin residues in animal tissues.

Determination of Aflatoxins in Animal Tissues

1981 ◽  
Vol 64 (4) ◽  
pp. 964-968
Author(s):  
Robert D Stubblefield ◽  
Odette L Shotwell
Keyword(s):  
Citric Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Determination Limit ◽  
Animal Tissues ◽  
Freeze Dried ◽  
Human Livers ◽  
Layer Chromatography

Abstract A method for the determination of aflatoxins in animal tissues has been developed, and applied successfully to beef, swine, chicken, and human livers, and to beef kidney, heart, spleen, muscle, and blood. Blended tissue is denatured with citric acid and extracted with dichloromethane on a wrist-action shaker. After filtration, the extract is partially purified on a silica gel column, and aflatoxins B1 and M1 are determined by 2-dimensional thin layer chromatography and densitometry. Recoveries of Bi and Mi added to meat tissues and blood were approximately 90 and 80%, respectively. The method gave results for a contaminated freeze-dried liver comparable to analyses by 3 other published meat tissue methods. The method is rapid and has a determination limit ≤0.1 ng/g. In addition, the method uses less toxic and smaller quantities of solvents and chemicals.

TLC Determination of Aquatic Humic Acids

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2567-2570 ◽  
Author(s):  
M. Kaštelan-Macan ◽  
Š. Cerjan-Stefanovic ◽  
D. Jalšovec
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Humic Acids ◽  
Visible Spectrum ◽  
Solvent System ◽  
Spectroscopic Methods ◽  
Precise Method ◽  
Adsorption Resin ◽  
Layer Chromatography

Humic acids are mostly determined by spectroscopic methods, although they are not accurate enough, because humic acids possess no maximum absorption at any wavelength in the ultraviolet and visible spectrum. In this contribution the accurate and precise method for aquatic humic acids determination, using thin layer chromatography, was worked out. Previously, humic acids were separated and preconcentrated -from the river water, using macroreticular adsorption resin Amberlite XAD-4, and subsequently determined by TLC. The Chromatographie system was: silicagel HF254(Merck, precoated plates 20 × 20 cm) and NH3 -acetone (60:40,v/v) as a solvent system. Quantitative determination was made by Camag– Turner 111 Fluorimeter, by measuring UV–quenching.

Determination of α-Hydroxy Acids in Foods by TLC and Manometric Measurement

1970 ◽  
Vol 53 (3) ◽  
pp. 621-623
Author(s):  
M Trop ◽  
I M Levinger
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Hydroxy Acids ◽  
Keto Acids ◽  
Alcoholic Extraction ◽  
Layer Chromatography ◽  
Manometric Measurement

Abstract An analytical method for determination of α-hydroxy and α-keto acids is developed based on separation of the acids from other components by acidic alcoholic extraction and by adsorption on and elution from an anion exchange resin, followed by thin layer chromatography. Acids are identified by eerie ammonium nitrate reagent sprayed on the chromatogram. Quantitative determination is performed by manometric measurement of gas released in reaction with that reagent.

Rapid Thin Layer Chromatographic Determination of Aflatoxin M1 in Powdered Milk

1985 ◽  
Vol 68 (5) ◽  
pp. 952-954
Author(s):  
Maria Luisa Serralheiro ◽  
Maria Lurdes Quinta
Keyword(s):  
Aqueous Solution ◽  
Carbon Tetrachloride ◽  
Thin Layer ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Methanol Solution ◽  
Aflatoxin M1 ◽  
Powdered Milk ◽  
Layer Chromatography

Abstract A method has been developed for the detection of aflatoxin Mi in milk. The toxin is extracted with chloroform, the extract is evaporated, and the residue is partitioned between carbon tetrachloride and an aqueous saline-methanol solution. The toxin is once again extracted with chloroform from the methanol solution and analyzed by thin layer chromatography. The limit of detection of Mi in powdered milk is 0.5 μg/ kg; recoveries of added Mj are about 83%. The limit of detection can be improved to 0.3 μg/kg if the plate is sprayed with an aqueous solution of H2S04 after development.

Sign in / Sign up

Export Citation Format

Share Document