Report on Multiresidue Methods (Interlaboratory Studies)

Abstract Two kinds of problems associated with developing standardized procedures for the safety evaluation of new compounds are identified. The first of these is the question of the desirability of using such standard methods. It is concluded that a basic set of procedures is to be recommended, but this should be supplemented with special tests as may be indicated. The second problem is connected with the technical difficulties of any given type of assay and is normally dealt with in terms of the state of the art at the time. Assays by the inhalation route tend to be custom designed and do not follow standard protocols. One of the causes of this situation is the propensity of individual investigators to design de novo the equipment used to effect exposure of animals to airborne substances. Second, some confusion exists because investigators do not always appreciate that the concentration-time product is not the same as the true dose received by the exposed subjects and this may lead to anomalies when dose-response relationships are being characterized. It is suggested that interlaboratory studies be undertaken to ascertain the variability that might be expected in independent assays of inhalation toxicity.

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Results from Two Interlaboratory Comparison Tests Organized in Germany and at the EU Level for Analysis of Acrylamide in Food

Journal of AOAC International ◽

10.1093/jaoac/88.1.292 ◽

2005 ◽

Vol 88 (1) ◽

pp. 292-298 ◽

Cited By ~ 15

Author(s):

Horst Klaffke ◽

Carsten Fauhl ◽

Wolfgang Mathar ◽

Richard Palavinskas ◽

Reiner Wittkowski ◽

...

Keyword(s):

Interlaboratory Comparison ◽

Gas Chromatography Mass Spectrometry ◽

Complex Matrix ◽

Joint Research ◽

Research Activities ◽

Federal Institute ◽

Interlaboratory Studies ◽

Directorate General ◽

The Eu

Abstract After the publication of high levels of acrylamide (AA) in food, many research activities started all over the world in order to determine the occurrence and the concentration of this substance in various types of food. As no validated methods were available at that time, interlaboratory studies on the determination of AA in food were of the highest priority. Under the boundary conditions of applying well-established evaluation schemes, the results of 2 studies conducted by the Federal Institute for Risk Assessment (BfR) in Germany and by the European Commission's Directorate General Joint Research Center (JRC) exhibited an overall acceptable performance of the participants in these studies. Nevertheless, many laboratories showed problems in determining AA in food with a complex matrix such as cocoa. The results of analysis also showed a broader variation of AA for samples with low AA concentrations and indicated a bias of the results obtained by gas chromatography–mass spectrometry without derivatization. Improvements of the performance of some laboratories appeared to be necessary.

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