Multiplex-Polymerase Chain Reaction Assay for the Authentication of the Mackerel Scomber colias in Commercial Canned Products
2006 ◽
Vol 89
(3)
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pp. 708-711
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Keyword(s):
Abstract A multiplex-polymerase chain reaction (PCR) system was developed for the authentication of the mackerel Scomber colias in commercial canned products. This novel method consists of an S. colias-specific fragment [159 base pairs (bp)] located in the nontranscribed spacer (NTS) sequence, and a Scomber genus-specific PCR product in the 5S rRNA gene (196201 bp) as a positive amplification control. The system was assayed using 18 different canned products labeled as S. colias. A positive identification was made in all but one sample, revealing this methodology as a potential molecular tool for direct application in the authentication of S. colias canned products.
2002 ◽
Vol 65
(5)
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pp. 780-785
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2015 ◽
Vol 30
(1)
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pp. 43-50
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2006 ◽
Vol 89
(1)
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pp. 144-148
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2003 ◽
Vol 86
(4)
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pp. 764-767
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2005 ◽
Vol 81
(1)
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pp. 114
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2003 ◽
Vol 96
(3)
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pp. 885-891
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2019 ◽
Vol 19
(3)
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pp. 322-326
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2018 ◽
Vol 9
(3)
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