scholarly journals Binary HPLC-Diode Array Detector and HPLC-Evaporative Light-Scattering Detector Fingerprints of Methanol Extracts from the Selected Sage (Salvia) Species

2011 ◽  
Vol 94 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Mieczysław Sajewicz ◽  
Dorota Staszek ◽  
Łukasz Wojtal ◽  
Teresa Kowalska ◽  
Michał Ł Hajnos ◽  
...  
Keyword(s):  
Light Scattering ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Evaporative Light Scattering Detector ◽  
Methanol Extracts ◽  
Evaporative Light Scattering ◽  
Single Detector ◽  
Salvia Officinalis L ◽  
Binary Fingerprints

Abstract This study is focused on an important family of the sage (Salvia) species, with Salvia officinalis L. having a long-established position in European traditional medicine. Binary fingerprints (chromatographic profiles) of six different sage species were compared using HPLC coupled with two different detectors: the diode-array detector and the evaporative light-scattering detector. Advantages of using binary fingerprinting over single-detector fingerprinting are demonstrated and discussed, with selected examples. Experimental data are provided for a comparison of the chemical composition of sage samples originating from two harvesting seasons (2007 and 2008). A number of phytochemical standards (i.e., certain phenolic acids, flavonoids, and coumarin) were used that allowed identification and semiquantitative estimation of these particular compounds in the analyzed methanol extracts.

scholarly journals An effective solution to simultaneously analyze size, mass and number concentration of polydisperse nanoplastics in a biological matrix: asymmetrical flow field fractionation coupled with a diode array detector and multiangle light scattering

RSC Advances ◽  
2021 ◽  
Vol 11 (21) ◽  
pp. 12902-12906
Author(s):  
Xing-ling Luo ◽  
Ying-ting Wu ◽  
Ling-yan Zhang ◽  
Ke-xin Li ◽  
Tian-jiang Jia ◽  
...  
Keyword(s):  
Light Scattering ◽  
Flow Field ◽  
Number Concentration ◽  
Array Detector ◽  
Diode Array ◽  
Effective Solution ◽  
Diode Array Detector ◽  
Biological Matrix

AF4-DAD-MALS is proposed to separate polydisperse nanoplastics and detect their size, mass and number concentration in a biological matrix.

scholarly journals Analysis of the Mycosporine-Like Amino Acid (MAA) Pattern of the Salt Marsh Red Alga Bostrychia scorpioides

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 321
Author(s):  
Maria Orfanoudaki ◽  
Anja Hartmann ◽  
Julia Mayr ◽  
Félix L. Figueroa ◽  
Julia Vega ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Red Alga ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
The Past ◽  
Specific Species

This study presents the validation of a high-performance liquid chromatography diode array detector (HPLC-DAD) method for the determination of different mycosporine-like amino acids (MAAs) in the red alga Bostrychia scorpioides. The investigated MAAs, named bostrychines, have only been found in this specific species so far. The developed HPLC-DAD method was successfully applied for the quantification of the major MAAs in Bostrychia scorpioides extracts, collected from four different countries in Europe showing only minor differences between the investigated samples. In the past, several Bostrychia spp. have been reported to include cryptic species, and in some cases such as B. calliptera, B. simpliciuscula, and B. moritziana, the polyphyly was supported by differences in their MAA composition. The uniformity in the MAA composition of the investigated B. scorpioides samples is in agreement with the reported monophyly of this Bostrychia sp.

scholarly journals Clitorienolactones and Isoflavonoids of Clitorea ternatea Roots Alleviate Stress-Like Symptoms in a Reserpine-Induced Zebrafish Model

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4137
Author(s):  
Muhammad Afiq Ngadni ◽  
Muhammad Tayyab Akhtar ◽  
Intan Safinar Ismail ◽  
Anis Irfan Norazhar ◽  
Soo Yee Lee ◽  
...  
Keyword(s):  
Mental Illnesses ◽  
Array Detector ◽  
Diode Array ◽  
Root Extract ◽  
Diode Array Detector ◽  
Aqueous Methanol ◽  
Zebrafish Model ◽  
Metabolite Profiles ◽  
Ultrahigh Performance Liquid Chromatography ◽  
As Stress

Clitorea ternatea has been used in Ayurvedic medicine as a brain stimulant to treat mental illnesses and mental functional disorders. In this study, the metabolite profiles of crude C. ternatea root extract (CTRE), ethyl acetate (EA), and 50% aqueous methanol (50% MeOH) fractions were investigated using ultrahigh-performance liquid chromatography–diode array detector–tandem mass spectrometry (UHPLC–DAD–MS/MS), while their effect on the stress-like behavior of zebrafish, pharmacologically induced with reserpine, was investigated. A total of 32 compounds were putatively identified, among which, a series of norneolignans, clitorienolactones, and various flavonoids (flavone, flavonol, isoflavone, and isoflavanone) was found to comprise the major constituents, particularly in the EA and 50% MeOH fractions. The clitorienolactones, presently unique to the species, were present in both the free and glycosylated forms in the roots. Both the EA and 50% MeOH fractions displayed moderate effects on the stress-induced zebrafish model, significantly decreasing freezing duration and elevating the total distance travelled and average velocity, 72 h post-treatment. The results of the present study provide further evidence that the basis for the use of C. ternatea roots in traditional medicine to alleviate brain-related conditions, such as stress and depression, is attributable to the presence of clitorienolactones and the isoflavonoidal constituents.

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