Simultaneous determination of bioactive constituents in Danggui Buxue Tang for quality control by HPLC coupled with a diode array detector, an evaporative light scattering detector and mass spectrometry

2007 ◽  
Vol 389 (2) ◽  
pp. 571-580 ◽  
Author(s):  
Ling Yi ◽  
Lian-Wen Qi ◽  
Ping Li ◽  
Yi-Han Ma ◽  
Yong-Jing Luo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Bioactive Constituents ◽  
Evaporative Light Scattering Detector ◽  
Danggui Buxue Tang ◽  
Evaporative Light Scattering

scholarly journals Binary HPLC-Diode Array Detector and HPLC-Evaporative Light-Scattering Detector Fingerprints of Methanol Extracts from the Selected Sage (Salvia) Species

2011 ◽  
Vol 94 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Mieczysław Sajewicz ◽  
Dorota Staszek ◽  
Łukasz Wojtal ◽  
Teresa Kowalska ◽  
Michał Ł Hajnos ◽  
...  
Keyword(s):  
Light Scattering ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Evaporative Light Scattering Detector ◽  
Methanol Extracts ◽  
Evaporative Light Scattering ◽  
Single Detector ◽  
Salvia Officinalis L ◽  
Binary Fingerprints

Abstract This study is focused on an important family of the sage (Salvia) species, with Salvia officinalis L. having a long-established position in European traditional medicine. Binary fingerprints (chromatographic profiles) of six different sage species were compared using HPLC coupled with two different detectors: the diode-array detector and the evaporative light-scattering detector. Advantages of using binary fingerprinting over single-detector fingerprinting are demonstrated and discussed, with selected examples. Experimental data are provided for a comparison of the chemical composition of sage samples originating from two harvesting seasons (2007 and 2008). A number of phytochemical standards (i.e., certain phenolic acids, flavonoids, and coumarin) were used that allowed identification and semiquantitative estimation of these particular compounds in the analyzed methanol extracts.

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