scholarly journals A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Minoy Cristobal ◽  
Jessica P Acosta ◽  
Su A Lee ◽  
Hans H Stein

Abstract AbstractThree experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of amino acids (AA), concentration of metabolizable (ME), and standardized total tract digestibility (STTD) of P in a new source of distillers dried grains with solubles (DDGS; ProCap DDGS) are greater than in conventional de-oiled DDGS. In experiment 1, nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicated 3 × 3 Latin square design with three diets and three periods for a total of nine replicate pigs per diet. Two diets included ProCap DDGS or de-oiled DDGS as the sole source of crude protein (CP) and AA. An N-free diet was used to determine the basal endogenous losses of CP and AA. Ileal digesta were collected on days 5 and 6 of each period after 4 d of adaptation to diets. Results from experiment 1 indicated that ProCap DDGS contained more CP and AA compared with de-oiled DDGS. The SID of all AA in ProCap DDGS was greater (P < 0.001) compared with de-oiled DDGS with the exception that the SID of Pro was not different between the two sources of DDGS. In experiment 2, 24 growing barrows (initial BW: 32.7 ± 3.1 kg) were housed individually in metabolism crates and used in a randomized complete block design and fed a corn-based diet or two diets containing corn and each source of DDGS with eight replicate pigs per diet. Fecal and urine samples were collected for 4 d after 7 d of adaptation. Results from experiment 2 indicated that concentration of ME in ProCap DDGS was greater (P < 0.05) compared with corn or de-oiled DDGS. In experiment 3, 32 growing barrows (initial BW: 20.2 ± 0.9 kg) were placed in metabolism crates and allotted to four diets with eight pigs per diet using a 2 × 2 factorial treatment arrangement. The de-oiled DDGS and ProCap DDGS were both included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Pigs were adapted to the diets for 5 d and fecal samples were collected for 4 d. Results from experiment 3 indicated that inclusion of phytase in the diet containing ProCap DDGS increased (P < 0.05) the STTD of P, but addition of phytase to the de-oiled DDGS diet did not increase STTD of P (interaction, P < 0.001), but the STTD of P was greater (P < 0.05) in de-oiled DDGS compared with ProCap DDGS. In conclusion, ProCap DDGS has greater SID of AA and contains more ME, but has reduced STTD of P compared with conventional de-oiled DDGS.

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 148-148
Author(s):  
Jessica P Acosta Medellin ◽  
Su A Lee ◽  
Hans H Stein

Abstract The objective of this experiment was to test if standardized ileal digestibility (SID) of AA in a new source of high-protein distillers dried grains with solubles (HP-DDGS) is greater than in conventional distillers dried grains with solubles (DDGS-CV). Nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicate 3 × 3 Latin Square design with 3 diets and 3 periods. Two diets included HP-DDGS or DDGS-CV as the sole source of CP and AA. A nitrogen-free diet was used to determine basal endogenous losses of CP and AA. The statistical model included ingredient as fixed effect and square, period, and animal as random effects. Results indicated that that HP-DDGS contained more CP and AA compared with DDGS-CV (Table 1). The SID of all AA in HP-DDGS were greater (P < 0.001) compared with DDGS-CV with the exception that the SID of Pro was not different between the 2 sources of DDGS. The greater concentrations of AA and values for SID of AA in HP-DDGS indicate that this new DDGS source has a very high quality and may be a great source of digestible AA in diets fed to pigs.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 188-189
Author(s):  
Vinicius R C Paula ◽  
Natália C Milani ◽  
Cândida P F Azevedo ◽  
Leury J Souza ◽  
Anderson A Sedano ◽  
...  

Abstract The objective of this study was to determine the apparent and standardized ileal digestibility (AID and SID) of crude protein (CP) and amino acids (AA) of a corn distillers dried grains with solubles (DDGS) from USA (UDG), a corn bran with solubles from Brazil (CBS) and high protein corn distillers dried grains from USA and Brazil (UHP and BHP), in growing pigs. Fifty crossbred barrows (46.2±5.3kg) were fed a semi-purified N-free diet, used to determine endogenous N losses, or four diets composed of 40% of each ingredient, as the only source of N, substituting for cornstarch in N-free diet. Animals were fed at 2.8 x maintenance (110 kcal of DE per kg of BW0.75) for 9 d and on the 10th d were euthanized for ileal digesta collection. TiO2 was used as an indigestible marker (0.3%) for digestibility calculations. A randomized block design was used, with 10 replicates, using the pig as the experimental unit, and results submitted to orthogonal contrast test. The CBS, UDG, BHP and UHP contained (as-fed basis) 13.9, 25.8, 42.9 and 34.9% CP; 9.0, 6.4, 10.3 and 7.3% EE; 40.8, 40.2, 32.7 and 47.5% NDF; 4.51, 4.53, 5.30 and 4.90 Mcal/kg GE; and 0.40, 0.73, 1.37 and 1.00% Lys, respectively. The AID of CP, Arg, His, Ile, Leu, Lys, Thr and Val; and the SID of His, Leu, Lys, and Val of BHP were 8 to 36% greater (P < 0.05) than those from UHP. The AID of CP, Arg, Ileu, Leu, Phe, Thr, and Trp; and SID of CP, Arg, Phe, and Thr of UDG were 9 to 45% greater (P < 0.05) than those of CBS. In conclusion, BHP had a greater digestibility of most AA than UHP, while the CBS evaluated had lower nutritional value than the UDG source.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 61-61
Author(s):  
Chan Sol Park ◽  
Ayodeji S Aderibigbe ◽  
Gary Hayen ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to evaluate the digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in dried yeast (DY) and soybean meal (SBM) fed to pigs. In Exp. 1, 30 barrows with an initial body weight (BW) of 20.7 ± 1.01 were assigned to 5 diets in a randomized complete block design with period and BW as blocking factors. A basal diet was prepared to contain corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5 or 10 g/kg DY or SBM at the expense of energy-contributing ingredients in the basal diet to estimate the DE and ME in test ingredients by regression analysis. On a dry matter basis, estimated DE and ME in DY were 4,022 and 3,352 kcal/kg, respectively, and those in SBM were 3,876 and 3,601kcal/kg, respectively. There was no difference in estimated DE or ME between DY and SBM. In Exp. 2, 21 barrows (initial BW = 20.0 ± 1.31 kg) surgically fitted with T-cannulas at the distal ileum were assigned to 3 diets in a randomized complete block design with BW as a blocking factor. Two diets were prepared to contain DY or SBM as the sole source of nitrogen, and a nitrogen-free diet was prepared to determine the basal ileal endogenous losses of AA. The SID of AA, except for Gly and Pro, in SBM were greater (P < 0.05) than in DY. The SID of indispensable AA in DY ranged from 64.7% for Thr to 86.1% for Arg, whereas those in SBM ranged from 84.8% for Thr to 92.3% for Arg. In conclusion, energy values in DY was comparable with SBM, but the SID of most AA in DY were less than in SBM.


Author(s):  
Jimena A Ibagon ◽  
Su A Lee ◽  
Hans H Stein

Abstract The objective was to test the hypothesis that there is no effect of origin or processing procedure on the standardized ileal digestibility (SID) of amino acids (AA) and crude protein (CP) in sunflower co-products. Six sources of sunflower meal (SFM) and 1 source of sunflower expellers (SFE) were obtained from Ukraine, Italy, Hungary, and the U.S. Each source of SFM or SFE was the only source of CP and AA in 1 diet and an N-free diet was also used for a total of 8 diets. Eight barrows (body weight: 28.5 ± 2.4 kg) had a T-cannula installed in the distal ileum and were allotted to 1 of the 8 diets using an 8 × 8 Latin square design with 8 periods. The Lys:CP ratio in the 6 sources of SFM ranged from 3.10 to 3.96% with CP concentrations ranging from 27.34 to 36.75%. Crude protein in SFE was 26.87% and the Lys:CP ratio was 3.51%. Concentrations of acid hydrolyzed ether extract in the 6 sources of SFM ranged from 0.60 to 3.11%, but SFE contained 8.77%. Results indicated that the SID of CP was lower (P < 0.05) in SFM from Hungary compared with all other sources of SFM except for 1 of the sources from Ukraine. There were no differences in the SID of Lys, Met, and Trp among sources of SFM, but for most of the remaining indispensable AA, the SFM from Hungary had less (P < 0.05) SID than the other sources. However, only few differences in SID of indispensable AA were observed among the other sources of SFM, but the SID of CP and all AA except Trp was greater (P < 0.05) in SFE compared with SFM. In conclusion, there were only few differences in the SID of the first limiting AA among SFM obtained from Ukraine, Hungary, Italy, and the U.S., but the SID of CP and AA was greater in SFE than in SFM indicating that processing of sunflower seeds influence the nutritional value.


2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Maryane S F Oliveira ◽  
John K Htoo ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) in corn, wheat, and wheat middlings obtained using the direct procedure are not different from values obtained using the difference procedure. Sixteen ileal-cannulated barrows (initial BW: 69.5 ± 5.0 kg) were allotted to a replicated 8 × 4 Youden Square design with 8 diets and 4 periods. Each period consisted of 5 d of adaptation to the diet and 2 d of collection of ileal digesta. Four diets were based on soybean meal (SBM), corn, wheat, or wheat middlings as the only AA-containing ingredients. Three additional diets were based on a mixture of SBM and corn, SBM and wheat, or SBM and wheat middlings, and an N-free diet was also used. The apparent ileal digestibility (AID) and the SID of crude protein (CP) and AA for the 4 diets containing SBM, corn, wheat, or wheat middlings as the sole source of AA were calculated using the direct procedure. The AID and SID of CP and AA for the 3 mixed diets containing SBM and corn, wheat, or wheat middlings were also calculated and the contribution of digestible AA from SBM was subtracted from the AID or SID values for the diets. The AID or SID of AA in corn, wheat, and wheat middlings were subsequently calculated by difference. Results indicated that the AID values for a few AA were lower (P < 0.05) if the direct procedure was used instead of the difference procedure, regardless of ingredient. The AID of Trp was greater in corn and wheat middlings, and the SID of Trp in corn and wheat middlings tended to be greater, if the direct procedure rather than the difference procedure was used, but that was not the case for wheat (interaction, P < 0.05 and P < 0.10, respectively). However, for all other indispensable AA, and for most of the dispensable AA, the SID of AA in corn, wheat, and wheat middlings was not different between the difference procedure and the direct procedure. Therefore, values for SID of AA in cereal grains and fiber-rich ingredients may be determined using either the direct or the difference procedure.


Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 933 ◽  
Author(s):  
Su Hyun An ◽  
Jung Yeol Sung ◽  
Hwan-Ku Kang ◽  
Changsu Kong

The aim was to test additivity of apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AAs) in mixed diets for Ross 308 broiler chickens. Two hundred and eighty-eight, 20-d-old male broiler chickens were assigned to one of six diets, with six birds per cage using a randomized complete block design. The diets consisted of a nitrogen-free diet, three diets containing corn, soybean meal (SBM), and corn distillers dried grains with solubles (CDDGS) as the sole source of nitrogen, respectively, and two mixed diets containing corn, SBM or CDDGS. Chromic oxide was added to the diets as an indigestible index. On day 24, birds were euthanized for ileal digesta collection. Relative proportion of the basal endogenous loss of AAs to total ileal outflow of AAs in corn was greater (p < 0.05) than that of SBM and CDDGS. For the corn-SBM and corn-SBM-CDDGS mixed diets, the AID of AAs differed (p < 0.05) from the predicted values. No difference was observed between the measured and predicted SID of AA. In conclusion, the SID of AAs is more additive in mixed diets containing corn, SBM, or CDDGS compared to AID values.


2019 ◽  
Vol 3 (2) ◽  
pp. 641-653
Author(s):  
Shelby M Curry ◽  
Laia Blavi ◽  
Julian Wiseman ◽  
Hans H Stein

Abstract Two experiments were conducted to compare the standardized ileal digestibility (SID) of AA by growing pigs in European distillers dried grains with solubles (DDGS) produced from wheat, maize, or wheat–maize mixtures and to test the effect of increasing the inclusion levels of wheat DDGS on growth performance of growing-finishing pigs fed diets balanced for NE and SID Lys. In Exp. 1, 12 barrows (initial BW: 23.0 ± 2.2 kg) were surgically equipped with a T-cannula in the distal ileum and randomly allotted to a replicated 6 × 6 Latin square design with six diets and six periods. Five sources of European DDGS were used: wheat DDGS from 2011, wheat DDGS from 2012, wheat-80 DDGS (80% wheat and 20% maize), wheat-70 DDGS (70% wheat and 30% maize), and maize DDGS. Each diet contained one source of DDGS as the sole source of AA and an N-free diet was used to determine basal endogenous losses of AA. Results indicated that the SID of CP was greater (P &lt; 0.05) in maize DDGS compared with wheat DDGS from 2011, wheat DDGS from 2012, and wheat-70 DDGS. The SID of all indispensable AA except Trp was also greater (P &lt; 0.05) in maize DDGS compared with all other DDGS sources used in this experiment. For Trp, the SID in wheat-80 DDGS, wheat DDGS from 2011, and wheat DDGS from 2012 were not different from maize DDGS, but were greater (P &lt; 0.05) than in wheat-70 DDGS. The SID for all indispensable AA except Ile and Trp in wheat-70 DDGS were not different from the values calculated for wheat DDGS from 2011 and wheat DDGS from 2012, and no differences between SID values for AA in wheat DDGS from 2011 and wheat DDGS from 2012 were observed. In Exp. 2, 36 growing pigs (initial BW: 38.3 ± 1.97 kg) were randomly allotted to one of four dietary treatments (one pig/pen and nine replicate pigs/treatment) in a 2-phase feeding program (35 to 65, and 35 to 105 kg BW). The four dietary treatments included diets containing 0%, 10%, 20%, or 30% wheat DDGS. Results indicated that there was no effect of wheat DDGS on pig growth performance or carcass quality. However, addition of wheat DDGS increased linearly (P &lt; 0.015) the indole concentration in the carcasses of the pigs. In conclusion, the SID of AA in maize DDGS produced in Europe is greater than in European wheat DDGS and DDGS produced from mixtures of wheat and maize, but inclusion of 30% wheat DDGS in diets fed to growing-finishing pigs did not affect growth performance or carcass quality.


2016 ◽  
Vol 56 (12) ◽  
pp. 2023
Author(s):  
Santi D. Upadhaya ◽  
Je-hoon Ryu ◽  
Kyung-il Kang ◽  
Seong-Jun Cho ◽  
In Ho Kim

Apparent ileal digestibility and standardised ileal digestibility of energy, dry matter, nitrogen and amino acids were evaluated in five cannulated [(Landrace × Yorkshire) × Duroc)] barrows with an initial bodyweight of 27.1 ± 0.58 kg in a 5 × 5 Latin square design. Pigs were fed unfermented soybean meal (USM) as control and fermented soybean meal A, B and C (FSMA, FSMB and FSMC, respectively), with 52.4%, 62.0% and 71.8% protein solubility, respectively, and a nitrogen-free diet. The nitrogen-free diet was used to determine basal endogenous losses of crude protein and amino acids. The apparent ileal digestibility of dry matter and nitrogen was greater (P < 0.05) in FMSC and tended to improve in FSMA and FSMB compared with the control. The apparent ileal digestibility of isoleucine, phenylalanine and valine was greater in all fermented soybean meal whereas that of lysine was greater (P < 0.05) in FSMC compared with USM. Likewise, the apparent ileal digestibility of aspartic acid and proline was greater (P < 0.05) in FSMA and FSMC. The standardised ileal digestibility of crude protein and lysine was higher (P < 0.05) in FSMC and tended to improve in FSMA and FSMB whereas the standardised ileal digestibility of isoleucine was higher (P < 0.05) in FSMA, FSMB and FSMC compared with USM. In conclusion, fermentation of soybean meal showed better digestibility compared with USM regardless of its protein solubility values.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 65-65
Author(s):  
Chan Sol Park ◽  
Victor Naranjo ◽  
Ariane Helmbrecht ◽  
John K Htoo ◽  
Olayiwola Adeola

Abstract This study was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in hydrolyzed feather meal (HFM), flash dried poultry protein (FDPP), poultry meal (PM), and meat and bone meal (MBM) fed to broiler chickens and pigs. Four semi-purified diets and a nitrogen-free diet were prepared. Exp. 1 was conducted with 416 male broiler chickens (initial body weight [BW] = 705 ± 100.0 g) at d 18 post-hatching in a randomized complete block design. Birds were fed diets for 5 d and then euthanized to collect ileal digesta. In Exp. 2, ten barrows (initial BW = 22.1 ± 1.59 kg) surgically fitted with T-cannulas at the distal ileum were assigned to duplicate 5 × 4 incomplete Latin square design with 5 diets and 4 periods. There were 5-d adaptation and 2-d ileal digesta collection periods in each experimental period. Data were analyzed as a 2 × 4 factorial arrangement with the effects of species (i.e., broiler chickens and pigs) and four experimental diets. There were interactions (P < 0.05) in the SID of His, Lys, Thr, Trp, and Val between diets and species. In broiler chickens, the SID of Lys in MBM was greater (P < 0.05) than HFM and FDPP but was not different from PM. Pigs fed MBM had greater (P < 0.05) SID of Lys than HFM, FDPP, and PM. In broiler chickens, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was less (P < 0.05) than MBM. In pigs, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was not different from MBM. In conclusion, differences in the SID of AA among animal protein sources were affected by species of monogastric animals.


2020 ◽  
Vol 4 (2) ◽  
pp. 708-714
Author(s):  
Carly M Rundle ◽  
Valeria Artuso-Ponte ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that a preparation of isoquinoline alkaloids (IQ) obtained from Macleaya cordata and added to corn-soybean meal diets increases the apparent ileal digestibility (AID) of amino acids (AA), crude protein (CP), starch, and acid hydrolyzed ether extract (AEE) when fed to young growing pigs. Thirty-two ileal cannulated barrows (initial body weight = 12.19 ± 1.38 kg) were allotted to a randomized complete block design with four diets and eight replicate pigs per diet. Diets were supplemented with 0, 90, 180, or 360 mg/kg IQ and with 0.40% chromic oxide. Diets were fed for 27 d and ileal digesta were collected on days 13 and 14 (period 1) and on days 26 and 27 (period 2). Effects of IQ inclusions were analyzed using contrast statements, and differences between periods were analyzed using a repeated measures statement. A quadratic increase (P &lt; 0.05) in the AID of Thr, Trp, Val, Pro, and Tyr was observed in period 1 as IQ was included in the diets, and AID of CP, Arg, His, Ile, Leu, Met, Phe, Thr, Trp, Val, Pro, and Tyr was greater in period 2 than in period 1 (P &lt; 0.05). In period 1, a quadratic increase (P &lt; 0.05) was observed for the AID of starch as IQ increased in the diet, but the AID of starch was less (P &lt; 0.05) in period 2 than in period 1. No differences among treatments or periods were observed for AID of AEE. Results indicate that inclusion of approximately 90 mg/kg of IQ in diets for weanling pigs may increase the AID of starch and some AA.


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