PSII-16 Effects of Oligosaccharide-based Polymer on Blood Profiles in Weanling Pigs Experimentally Infected with a Pathogenic E. coli

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on blood profiles of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Blood samples were collected before E. coli inoculation (d 0), and on d 2, 5, 8, and 11 post-inoculation (PI). Total and differential blood cell count were analyzed by CBC test. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Supplementation of oligosaccharide-based polymer linearly (P < 0.05) reduced white blood cell counts, neutrophils, eosinophils, and basophils on d 2 PI, and neutrophils on d 5 PI, compared with the control. No differences were observed in total and differential white blood cell counts among AGP and two oligosaccharide-based polymer treatments except that pigs fed with AGP had greater (P < 0.05) lymphocytes on d 2 PI compared with pigs fed with oligosaccharide-based polymer diets. Supplementation of low dose oligosaccharide-based polymer or AGP reduced (P < 0.05) red blood cell count and packed cell volume on d 2 PI, whereas inclusion of high dose oligosaccharide-based polymer or AGP reduced (P < 0.05) packed cell volume on d 5 PI, compared with the control. In conclusion, supplementation of oligosaccharide-based polymer may alleviate the systemic inflammation caused by F18 E. coli infection.

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THE NORMAL BLOOD CHEMISTRY OF THE BEAVER (CASTOR CANADENSIS): A. PACKED-CELL VOLUME, SEDIMENTATION RATE, HEMOGLOBIN, ERYTHROCYTE DIAMETER, AND BLOOD CELL COUNTS

Canadian Journal of Zoology ◽

10.1139/z58-026 ◽

1958 ◽

Vol 36 (3) ◽

pp. 279-283 ◽

Cited By ~ 5

Author(s):

W. D. Kitts ◽

May C. Robertson ◽

B. Stephenson ◽

I. McT. Cowan

Keyword(s):

Blood Cell ◽

Cell Count ◽

Cell Volume ◽

Sedimentation Rate ◽

Packed Cell Volume ◽

Cell Diameter ◽

Differential Count ◽

Red Cell ◽

Blood Cell Count ◽

Cell Counts

Packed-cell volume, sedimentation rate, hemoglobin, red blood cell count, white blood cell count, differential count, and red cell diameter have been determined on blood samples taken from 22 beavers. The various values obtained are in general comparable with those reported for the other rodents. Exceptions to this general agreement were noted in the much higher sedimentation rate and slightly larger red cell diameter of beaver blood.

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EFFECTS OF PROBIOTIC SACCHAROMYCES BOULARDII AND CO-TRIMOXAZOLE ON SOME HAEMATOLOGICAL PARAMETERS IN ADULT WISTAR RATS

African Journal of Health, Safety and Environment ◽

10.52417/ajhse.v2i2.158 ◽

2021 ◽

Vol 2 (2) ◽

pp. 55-62

Author(s):

P. A. Obazelu ◽

I. A. Aruomaren ◽

C. C. Nwangwu

Keyword(s):

Body Weight ◽

Blood Cell ◽

Cell Count ◽

Cell Volume ◽

White Blood Cell Count ◽

White Blood Cell ◽

Packed Cell Volume ◽

Saccharomyces Boulardii ◽

Total White Blood Cell ◽

Blood Cell Count

Saccharomyces boulardii is a probiotic yeast used as a nutritional supplement. Co-trimoxazole, is a broad-spectrum antimicrobial agent used in the prophylaxis and treatment of infections.The goal of this study was to evaluate the individual and interactive effects of probiotic Saccharomyces boulardii and co-trimoxazole on some haematological parameters in albino rats. Thirty (30) albino Wistar rats weighing 150-200g were divided into five groups; Basal, A, B, C, and D, with six animals in each group. Group A (control) was given a normal rat pellet (standardized feed) and clean water (pH 6.5- 8.5). Group B was administered 5.5mg/kg body weight of the commercial probiotics (Saccharomyces boulardii). Group C was administered 4.4mg/kg body weight of co-trimoxazole. Group D was administered 5.5mg/kg body weight of the commercial probiotics and 4.4mg/kg body weight of co-trimoxazole. All administrations were done using a gavage tube for 15 days. Blood samples were collected for analysis on days 0, 5 and 15. The results showed that Saccharomyces boulardii caused a significant increase (P<0.05) in total white blood cell count and packed cell volume of the treated groups at day 5. On day 15, the total white blood cell count (10.67±0.66), packed cell volume (43.23±0.47), platelet count (932±38.89) and mean platelet volume (8.53±0.38) showed a significant increase (P<0.05) in the probiotic group. The co-trimoxazole group showed a significant reduction (P<0.05) in the packed cell volume and total white blood cell count. This study shows that Saccharomyces boulardii has a positive effect on the haematological profile of the test animals.

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Assessment of White Blood Cell Count, Packed Cell Volume, Phagocytic Functions, Serum Albumin and Plasma Iron in HIV Infected Patients

Clinical Immunology ◽

10.1016/j.clim.2010.03.329 ◽

2010 ◽

Vol 135 ◽

pp. S110 ◽

Cited By ~ 2

Author(s):

Charles Onyenekwe ◽

Prince Ele ◽

Nkiruka Ukibe ◽

Michael Ezeani ◽

Clement Ezechukwu ◽

...

Keyword(s):

Blood Cell ◽

Serum Albumin ◽

Cell Count ◽

Cell Volume ◽

White Blood Cell Count ◽

White Blood Cell ◽

Packed Cell Volume ◽

Blood Cell Count ◽

Plasma Iron

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Effects of the tiletamine/zolazepam-xylazine-tramadol combination on plasma oxidative status and haematological indicators in miniature pigs

Acta Veterinaria Brno ◽

10.2754/avb201483020145 ◽

2014 ◽

Vol 83 (2) ◽

pp. 145-149 ◽

Cited By ~ 1

Author(s):

Sheng Jiang ◽

Hong-Gang Fan ◽

De-Zhang Lu ◽

Jin-Long Hou ◽

Xu-Dong Song ◽

...

Keyword(s):

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Blood Cell ◽

Cell Volume ◽

White Blood Cell ◽

Red Blood Cell ◽

Packed Cell Volume ◽

Cell Counts ◽

Blood Cell Counts ◽

Baseline Values

Oxidative stress in the body occurs when the production of free radicals overwhelms the antioxidant defence systems. The aim of this study was to examine the effects of a tiletamine/zolazepam-xylazine-tramadol combination on the antioxidant status, lipoperoxidase and blood cell indicators in eight miniature pigs. Baseline measures were recorded prior to anaesthesia, then the animals were anaesthetized with the combination of tiletamine/zolazepam (3.5 mg/kg), xylazine (1.32 mg/kg), and tramadol (1.8 mg/kg). Blood samples were collected from the anterior vena cava at 15, 30, 60, 90, 120 min, 24 h, and 72 h after anaesthesia. Plasma malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase concentrations were measured by colorimetry, and red blood cell counts, white blood cell counts, haemoglobin and packed cell volume were determined using an automated cell counter. The results showed that the concentration of malondialdehyde increased significantly at 30 and 60 min after the injection (P< 0.05), whereas glutathione peroxidase and catalase activity increased slightly (P> 0.05) then returned to baseline values after 90 min (P> 0.05). Superoxide dismutase activity increased significantly (P< 0.05) at 30 and 60 min, and then gradually decreased to baseline values after 90 min. Changes in red blood cell counts, haemoglobin and packed cell volume were not significant, while white blood cell count decreased significantly (P< 0.05) at 30 and 60 min. Our study is the first to demonstrate that tiletamine/zolazepam-xylazine-tramadol provide antioxidant effects, which may be proposed for alleviating the stress of examination and research at veterinary clinics or long-distance transportation.

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Serum Copper and Haematological Values of Sheep of Different Physiological Stages in the Dry and Wet Seasons of Central Trinidad

Veterinary Medicine International ◽

10.1155/2014/972074 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

A. Mohammed ◽

M. Campbell ◽

F. G. Youssef

Keyword(s):

Cell Volume ◽

Packed Cell Volume ◽

Serum Copper ◽

Wet Season ◽

Cell Counts ◽

Blood Cell Counts ◽

Age Related ◽

Physiological Stage ◽

White Blood Cell Counts ◽

Hematological Values

A study was conducted to determine serum copper (Cu) concentrations and hematological values of 174 sheep from four medium sized farms, pertaining to physiological stage, in the late dry and late wet seasons of Central Trinidad. Serum Cu was significantly lower in the dry than in the wet season (P<0.001) with a corresponding high percentage of samples with low Cu levels in the former. 31% of dry nonpregnant sheep exhibited a nonregenerative anaemia. Also, hemoglobin and packed cell volume values varied (P<0.001) including lymphocyte (P<0.01) counts, among growing animals compared with other physiological stages. Significant variations also occurred among neutrophil (P<0.05) and eosinophil (P<0.05) values in sheep. Highest haemoglobin and packed cell volume, white blood cell counts, and lymphocyte values in growing sheep compared with other stages were probably age related.

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Changes in Some Haematological Parameters of Pubertal Yankasa Rams ‎Fed Graded Levels of Dietary Protein Using Cotton Seed and Palm Kernel ‎Cake

Sahel Journal of Veterinary Sciences ◽

10.54058/saheljvs.v17i1.65 ◽

2020 ◽

Vol 17 (1) ◽

pp. 7-11

Author(s):

Taiwo Kamardeen Bello ◽

Balkisu Banke Oyelowo ◽

Raheela Hassan ◽

Brenda Oke ◽

Sheriff Yusuf Idris ◽

...

Keyword(s):

Blood Cell ◽

White Blood Cell ◽

Red Blood Cell ◽

Dietary Protein ◽

Total Protein ◽

Packed Cell Volume ◽

Crude Protein ◽

Cell Counts ◽

Blood Cell Counts ◽

White Blood Cell Counts

The effects of graded levels of dietary protein on some haematological parameters in pubertal Yankasa rams were studied. They were randomly divided into three treatment groups (A, B and C). Groups A, B and C received 10%, 15% and 20% crude protein respectively. All rams were fed a basal diet of hay (Digitaria spp) ad-libitum and given a supplement ration of concentrate mixture at 2% body weight/day. Blood was collected once weekly via jugular venepuncture into heparinised test-tubes, and transported to the laboratory for determination of red blood cell counts, packed cell volume, white blood cell counts and total protein for twelve weeks. The overall mean red blood cell values obtained were 4.2 ± 0.1 ×106 µl, 5.2 ± 0.1 106 µl and 5.06 ± 0.1 ×106 µl, for groups A, B and C respectively. The difference between values obtained for groups B and C were not significantly different (P > 0.05). Mean packed cell volumes obtained in this study revealed that rams fed 15% crude protein had significantly (P < 0.05) higher values (31.3 ± 0.9%) than those fed 10% (25.9± 0.7%) but similar to those fed 20% (30.33 ± 0.56%) crude protein. There were no significant (P > 0.05) differences in mean white blood cell counts and total protein concentrations between all groups of animals fed 10% CP. Feeding rams diets containing 15 % CP (cotton seed cake and palm kernel cake) gave better values in terms of packed cell volume and red blood cell counts, than feeding diets containing 10% and 20% C.P. While graded levels of dietary protein was shown to affect packed cell volume and total protein concentration, it had no significant effect on red blood cell and white blood cell counts.

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Complete Blood Cell Counts and Infection

PEDIATRICS ◽

10.1542/peds.68.2.305 ◽

1981 ◽

Vol 68 (2) ◽

pp. 305-305

Author(s):

Walt Karniski

Keyword(s):

Medical Student ◽

Blood Cell ◽

Cell Count ◽

Medical Practice ◽

Complete Blood Cell Count ◽

Blood Cell Count ◽

Cell Counts ◽

Blood Cell Counts ◽

Viral Illness

I remember reading Todd's article1 as a medical student and feeling that he had simplified medical practice considerably, by "proving" that a complete blood cell count (CBC) could accurately distinguish between any child with bacteremia and a child with a viral illness. The article by Baron and Fink2 brought that memory to mind again, and therefore necessitates words of caution. When considering the efficacy of a test, it is helpful to consider a two-by-two table as follows, in which the Baron and Fink data are presented:

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Fibrinogen, Viscosity and White Blood Cell Count Predict Myocardial, but not Cerebral Infarction: Evidence from the Caerphilly and Speedwell Cohort

Thrombosis and Haemostasis ◽

10.1055/s-0037-1613020 ◽

2002 ◽

Vol 87 (03) ◽

pp. 421-425 ◽

Cited By ~ 18

Author(s):

Janet Pickering ◽

Peter Elwood ◽

Antony Bayer ◽

Shah Ebrahim ◽

Ian Baker

Keyword(s):

Heart Disease ◽

Blood Cell ◽

Ischaemic Heart Disease ◽

White Blood Cell ◽

Plasma Viscosity ◽

Confounding Factors ◽

Blood Cell Count ◽

Cell Counts ◽

Blood Cell Counts ◽

White Blood Cell Counts

SummaryFibrinogen, plasma viscocity, and the white blood cell count predict ischaemic heart disease, but there is less certainty for their predictive power for ischaemic stroke. Studying stroke and ischaemic heart disease in the same cohort prospectively allows comparison of predictive strengths. The Caerphilly and Speedwell cohorts consist of a population sample of 4,860 men aged 45-59 years at recruitment who had baseline measurements of fibrinogen, plasma viscosity, and white blood cell counts. After 15-19 years of follow-up, men in the two cohorts experienced 312 ischaemic strokes and 557 ischaemic heart disease events. Mean fibrinogen, plasma viscosity and white blood cell counts differed significantly after adjustment for confounding factors between men with and without ischaemic heart disease, 0.25 g/l (95% CIs 0.18-0.32); 0.036 cp (95% CIs 0.027-0.044); 0.67 X 109/l (95% CIs 0.50-0.84) respectively. The same measurements showed no significant differences after adjustment for the same confounding factors for men with and without ischaemic stroke, 0.05 g/l (95% CIs -0.04-0.14); 0.008 cp (95% CIs -0.003-0.019); 0.16 X 109/l (95% CIs -0.06-0.38) respectively.

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Effects of storage duration on haematological parameters of the red eared slider - Trachemys Scripta Elegans

Veterinarska stanica ◽

10.46419/vs.51.6.3 ◽

2020 ◽

Vol 51 (6) ◽

pp. 639-644

Author(s):

Nejra Hadžimusić ◽

Dunja Rukavina ◽

Vedad Škapur ◽

Lejla Velić

Keyword(s):

Blood Cell ◽

Cell Count ◽

Cell Volume ◽

Packed Cell Volume ◽

Blood Cells ◽

Healthy Adult ◽

Haematological Parameters ◽

Blood Cell Count ◽

Storage Duration ◽

Blood Samples

Reptiles, especially turtles, are becoming increasingly popular as pets. The haematological evaluation of turtles is an irreplaceable diagnostic tool in veterinary practice. However, the morphologic distinctiveness of turtle blood limits the use of electronic cell-counting devices, making time-consuming, manual counting techniques and evaluation of blood smears necessary. Many samples are dispatched to a laboratory over long distances, where a delay of 24 h or more may occur. At weekends, this interval may exceed 48 h. The objective of the present study was to determine the effect of storage duration at refrigerator temperature (4 °C) on the counts of red blood cells (RBC) and white blood cells (WBC), and on the mean corpuscular volume (MCV) and packed cell volume (PCV) in blood samples from healthy adult red-eared sliders. Blood samples were collected by venipuncture from the occipital venous sinus from six apparently healthy adult red-eared sliders, aged 2 to 4 years. Blood samples were analysed immediately after sampling to obtain the baseline value (BV) of the red blood cell count, white blood cell count and packed cell volume percentage. Blood was stored at 4 °C and the haematological analyses were performed after 24h, 48h and 72h. The results showed the same level of stability for RBC and WBC count, and MCV values during 72 hours of storage at 4 °C and for PCV during 48 hours. Handling of blood samples, and duration of storage of the blood samples can significantly influence the results/values of haematological tests. Consequently, the obtained values of the determined haematological parameters of improperly stored or handled blood samples can give a misleading interpretation of the results on the animal’s health status.

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