scholarly journals Serum Copper and Haematological Values of Sheep of Different Physiological Stages in the Dry and Wet Seasons of Central Trinidad

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
A. Mohammed ◽  
M. Campbell ◽  
F. G. Youssef
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Serum Copper ◽  
Wet Season ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Age Related ◽  
Physiological Stage ◽  
White Blood Cell Counts ◽  
Hematological Values

A study was conducted to determine serum copper (Cu) concentrations and hematological values of 174 sheep from four medium sized farms, pertaining to physiological stage, in the late dry and late wet seasons of Central Trinidad. Serum Cu was significantly lower in the dry than in the wet season (P<0.001) with a corresponding high percentage of samples with low Cu levels in the former. 31% of dry nonpregnant sheep exhibited a nonregenerative anaemia. Also, hemoglobin and packed cell volume values varied (P<0.001) including lymphocyte (P<0.01) counts, among growing animals compared with other physiological stages. Significant variations also occurred among neutrophil (P<0.05) and eosinophil (P<0.05) values in sheep. Highest haemoglobin and packed cell volume, white blood cell counts, and lymphocyte values in growing sheep compared with other stages were probably age related.

PSII-16 Effects of Oligosaccharide-based Polymer on Blood Profiles in Weanling Pigs Experimentally Infected with a Pathogenic E. coli

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 167-168
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Seijoo Yang ◽  
Xunde Li ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cell Count ◽  
E Coli ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
White Blood Cell Counts ◽  
Blood Profiles

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on blood profiles of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Blood samples were collected before E. coli inoculation (d 0), and on d 2, 5, 8, and 11 post-inoculation (PI). Total and differential blood cell count were analyzed by CBC test. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Supplementation of oligosaccharide-based polymer linearly (P &lt; 0.05) reduced white blood cell counts, neutrophils, eosinophils, and basophils on d 2 PI, and neutrophils on d 5 PI, compared with the control. No differences were observed in total and differential white blood cell counts among AGP and two oligosaccharide-based polymer treatments except that pigs fed with AGP had greater (P &lt; 0.05) lymphocytes on d 2 PI compared with pigs fed with oligosaccharide-based polymer diets. Supplementation of low dose oligosaccharide-based polymer or AGP reduced (P &lt; 0.05) red blood cell count and packed cell volume on d 2 PI, whereas inclusion of high dose oligosaccharide-based polymer or AGP reduced (P &lt; 0.05) packed cell volume on d 5 PI, compared with the control. In conclusion, supplementation of oligosaccharide-based polymer may alleviate the systemic inflammation caused by F18 E. coli infection.

scholarly journals Effects of the tiletamine/zolazepam-xylazine-tramadol combination on plasma oxidative status and haematological indicators in miniature pigs

2014 ◽  
Vol 83 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Sheng Jiang ◽  
Hong-Gang Fan ◽  
De-Zhang Lu ◽  
Jin-Long Hou ◽  
Xu-Dong Song ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Blood Cell ◽  
Cell Volume ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Packed Cell Volume ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Baseline Values

Oxidative stress in the body occurs when the production of free radicals overwhelms the antioxidant defence systems. The aim of this study was to examine the effects of a tiletamine/zolazepam-xylazine-tramadol combination on the antioxidant status, lipoperoxidase and blood cell indicators in eight miniature pigs. Baseline measures were recorded prior to anaesthesia, then the animals were anaesthetized with the combination of tiletamine/zolazepam (3.5 mg/kg), xylazine (1.32 mg/kg), and tramadol (1.8 mg/kg). Blood samples were collected from the anterior vena cava at 15, 30, 60, 90, 120 min, 24 h, and 72 h after anaesthesia. Plasma malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase concentrations were measured by colorimetry, and red blood cell counts, white blood cell counts, haemoglobin and packed cell volume were determined using an automated cell counter. The results showed that the concentration of malondialdehyde increased significantly at 30 and 60 min after the injection (P< 0.05), whereas glutathione peroxidase and catalase activity increased slightly (P> 0.05) then returned to baseline values after 90 min (P> 0.05). Superoxide dismutase activity increased significantly (P< 0.05) at 30 and 60 min, and then gradually decreased to baseline values after 90 min. Changes in red blood cell counts, haemoglobin and packed cell volume were not significant, while white blood cell count decreased significantly (P< 0.05) at 30 and 60 min. Our study is the first to demonstrate that tiletamine/zolazepam-xylazine-tramadol provide antioxidant effects, which may be proposed for alleviating the stress of examination and research at veterinary clinics or long-distance transportation.

scholarly journals Changes in Some Haematological Parameters of Pubertal Yankasa Rams ‎Fed Graded Levels of Dietary Protein Using Cotton Seed and Palm Kernel ‎Cake

2020 ◽  
Vol 17 (1) ◽  
pp. 7-11
Author(s):  
Taiwo Kamardeen Bello ◽  
Balkisu Banke Oyelowo ◽  
Raheela Hassan ◽  
Brenda Oke ◽  
Sheriff Yusuf Idris ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Dietary Protein ◽  
Total Protein ◽  
Packed Cell Volume ◽  
Crude Protein ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
White Blood Cell Counts

The effects of graded levels of dietary protein on some haematological parameters in pubertal Yankasa rams were studied. They were randomly divided into three treatment groups (A, B and C). Groups A, B and C received 10%, 15% and 20% crude protein respectively. All rams were fed a basal diet of hay (Digitaria spp) ad-libitum and given a supplement ration of concentrate mixture at 2% body weight/day. Blood was collected once weekly via jugular venepuncture into heparinised test-tubes, and transported to the laboratory for determination of red blood cell counts, packed cell volume, white blood cell counts and total protein for twelve weeks. The overall mean red blood cell values obtained were 4.2 ± 0.1 ×106 µl, 5.2 ± 0.1 106 µl and 5.06 ± 0.1 ×106 µl, for groups A, B and C respectively. The difference between values obtained for groups B and C were not significantly different (P > 0.05). Mean packed cell volumes obtained in this study revealed that rams fed 15% crude protein had significantly (P < 0.05) higher values (31.3 ± 0.9%) than those fed 10% (25.9± 0.7%) but similar to those fed 20% (30.33 ± 0.56%) crude protein. There were no significant (P > 0.05) differences in mean white blood cell counts and total protein concentrations between all groups of animals fed 10% CP. Feeding rams diets containing 15 % CP (cotton seed cake and palm kernel cake) gave better values in terms of packed cell volume and red blood cell counts, than feeding diets containing 10% and 20% C.P. While graded levels of dietary protein was shown to affect packed cell volume and total protein concentration, it had no significant effect on red blood cell and white blood cell counts.  

scholarly journals Age at weaning and the endocrine response to stress

2003 ◽  
Vol 55 (1) ◽  
pp. 1-7 ◽  
Author(s):  
A.R. Bueno ◽  
R. Rasby ◽  
E.T. Clemens
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
White Blood Cell ◽  
Packed Cell Volume ◽  
Blood Chemistry ◽  
Blood Constituents ◽  
Cell Counts ◽  
Calf Performance ◽  
White Blood Cell Counts ◽  
Differential White Blood Cell

Seventy-five Angus x MARC II crossbred heifers were used to assess the effects of age at weaning on calf performance and blood constituents. Calves were weaned at 150, 210, or 270 days of age with comparisons made to their non-weaned counterparts. Changes in blood chemistry and hematology (cortisol, glucose, T3, total proteins, packed cell volume and the differential white blood cell count) were recorded on the day of weaning (day-0), and at 2, 7, 14, and 28 days post-weaning. Calves weaned at 210 days of age presented the highest plasma cortisol, glucose, and total protein concentrations during their early post-weaning period. Calves weaned at 150 days of age had significantly lower plasma T3 values. Packed cell volume and differential white blood cell counts showed no relationship to age at weaning. Calves weaned at 210 days of age presented the most rapid post-weaning growth performance, relative to the 150 or 270 days of age weaned calves.

scholarly journals Haematological and biochemical parameters of Kano brown bucks fed graded levels of potash treated neem (Azadirachta indica) leaf meal-based diets

2020 ◽  
Vol 46 (1) ◽  
pp. 196-205
Author(s):  
Y. Garba ◽  
Z. H. Ibrahim
Keyword(s):  
Standard Deviation ◽  
Blood Cell ◽  
Azadirachta Indica ◽  
Packed Cell Volume ◽  
Biochemical Parameters ◽  
Leaf Meal ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
The Mean ◽  
White Blood Cell Counts

The haematological and serum profile of Kano brown bucks fed neem (Azadirachta indica) leaf meal was investigated for 63 days. Twelve growing Kano Brown bucks were randomly allotted to four dietary treatments with three animals per treatments in a completely randomized block design. Treatment A was the control diet without neem leaf meal while treatments B, C and D had 10%, 20% and 30% potash-treated neem leaf inclusion levels respectively. Blood samples were collected at the end of the experiment for analysis of haematology and biochemical parameters. The mean value of packed cell volume, haemoglobin, red blood cell counts and white blood cell counts were significantly (p>0.05) different across all treatments. The mean and standard deviation were 29.00, 90.10; 10.06, 0.39; 9.50, 0.55; 10.5, 1.11; 30.20, 0.08; 10.41, 0.85 and 33.02, 0.45 for PCV, Hg, RBC, WBC, MCV, MCH and MCHC respectively. Mean corpuscular volume was significantly (p<0.05) higher for animals fed 10% inclusion of the tested material ranging from (25.50 - 36.00 fl). Eosinophils, monocytes and basophils were not significantly different across alltreatments while, lymphocytes and neutrophils were statistically significant (p<0.05) across all treatments with mean values of 43.29% and 56.75% respectively. The serum sodium ranged from (145.00 - 150.00 mmol/L), potassium ranged from (4.975 - 5.150 mmol/L) across all treatments. The creatinine value ranged from (85.50 - 103.5 mmol/L) total protein, albumin and globulin showed significant (p<0.05) difference across all treatments. The standard deviation was 0.003, 0.01, 0.02 and 0.18 respectively. Aspartate Aminotransferase, Alanine Aminotransferase and Alkaline Phosphatase were significantly (p<0.05) different across all treatments. The study revealed that potash-treated neem leaf meal could be used as feed for small ruminants, without any deleterious effect on performance and blood profile. It is concluded that dietary inclusion of potash treated neem leaf meal at 10 and 20% improved haemoglobin, packed cell volume and white blood cell counts. 

An Alternative Elastase-mediated Degradation of Fibrinogen and Fibrin Observed in a Patient with Herpes Simplex Encephalitis and Pneumonia

1996 ◽  
Vol 76 (02) ◽  
pp. 184-186 ◽  
Author(s):  
Kenji lijima ◽  
Fumiyo Murakami ◽  
Yasushi Horie ◽  
Katsumi Nakamura ◽  
Shiro Ikawa ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Blood Cell ◽  
White Blood Cell ◽  
Herpes Simplex Encephalitis ◽  
Pmn Elastase ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Sds Page ◽  
White Blood Cell Counts ◽  
Pmn Élastase

SummaryA 74-year-old female developed pneumonia following herpes simplex encephalitis. Her white blood cell counts reached 28,400/μl, about 90% of which consisted of granulocytes. The polymorphonuclear (PMN) elastase/α1-arantitrypsin complex levels increased and reached the maximum of 5,019 ng/ml, indicating the release of a large amount of elastase derived from the granulocytes. The mechanism of PMN elastase release was most likely to be granulocyte destruction associated with phagocytosis. The cleavage of fibrinogen and fibrin by PMN elastase, independent of plasmin, was indicated by the presence of the fragments in immunoprecipitated plasma from the patient corresponding to elastase-induced FDP D and DD fragments and the absence of fragments corresponding to plasmin-induced FDP D and DD fragments on SDS-PAGE. These findings suggested that the large amount of PMN elastase released from the excessive numbers of granulocytes in this patient with herpes simplex encephalitis and pneumonia, induced the cleavage of fibrinogen and fibrin without the participation of plasmin.

scholarly journals Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1478
Author(s):  
Lorenzo G. T. M. Segabinazzi ◽  
Giorgia Podico ◽  
Michael F. Rosser ◽  
Som G. Nanjappa ◽  
Marco A. Alvarenga ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Platelet Rich Plasma ◽  
Upright Position ◽  
Platelet Counts ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Platelet Concentration ◽  
Manual Methods ◽  
White Blood Cell Counts ◽  
Over Time

In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.

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