PSV-8 Effects of Air Exposure and Agitation on Quality of Stored Boar Semen Samples

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 210-211
Author(s):  
Kayode B Balogun ◽  
Kara R Stewart
Keyword(s):  
Bacterial Growth ◽  
Semen Quality ◽  
Short Term ◽  
Air Exposure ◽  
Storage Container ◽  
Semen Volume ◽  
Semen Storage ◽  
Boar Semen

Abstract Recent adoption of post cervical artificial insemination facilitates lowered concentrations of semen inseminated. In order to achieve this reduced concentration, the volume of the insemination dose has been reduced and replaced with additional air in the semen storage container. The objective of this study was to investigate the effects of semen volume, air contact inside semen dose tubes, daily agitation of semen doses and extender type on semen quality, thermo-resistance and bacterial growth in extended boar semen doses over 7 days of liquid storage at 17 C. Ejaculates from 4 terminal cross-bred boars were collected for 4 weeks and used in the 3 x 2 x 2 factorial study. The effects of treatment (CON: 80ml doses sealed at the top of the tube; 40HIGH: 40ml doses sealed at top of tube; and 40LOW: 40ml doses sealed at top of the liquid), extender type (long-term vs short-term), and agitation (agitated vs not agitated) were investigated. The result of the study revealed that motility (P=0.014) and viability (P=0.007) in 40HIGH were lower than CON. pH (P< 0.001) was higher in 40HIGH compared to CON. Agitation did not impact motility (P=0.541), viability (P=0.406) or morphology (P=0.970) while long-term extender maintained higher motility (P=0.034), viability (P< 0.001) and normal acrosomes (P< 0.001) compared to short-term extender. VAP (P=0.039) of 40HIGH was lower than CON in a thermo-resistance test. Bacteria were cultured on both sheep’s blood and MacConkey agar and neither treatment (P=0.798; 0.766) nor agitation (P=0.396; 0.476) impacted bacterial growth in this study. In conclusion, semen doses prepared with 80mL or 40mL of total volume with minimal air contact in the tubes yield more desirable semen quality, as air contact negatively impacts boar semen pH and sperm motility. Additionally, regardless of volume or air exposure, daily agitation of boar semen doses did not affect semen quality.

Value of semen parameters, with special reference to TNF-α, in predicting the quality of boar semen after short-term storage

2013 ◽  
Vol 61 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Janko Mrkun ◽  
Marjan Kosec ◽  
Petra Zrimšek
Keyword(s):  
Semen Quality ◽  
Semen Parameters ◽  
Short Term ◽  
Progressive Motility ◽  
Tnf Α ◽  
Semen Storage ◽  
Boar Semen ◽  
Α Level ◽  
Term Storage

The aim of this study was to address the question whether changes in boar semen quality after short-term storage could be predicted on the basis of standard semen parameters and TNF-α level determined on the day of semen collection under commercial conditions. Progressive motility showed the highest positive correlation with morphology on day 0 of collection, and progressive motility on day 3 (P < 0.05) showed a negative correlation with acrosome abnormalities (P < 0.05). According to the area under receiver operating characteristics (ROC) curves (AUCs), progressive motility could also be used in predicting semen quality after 3 days of storage (AUC > 0.5; P < 0.05). TNF-α in seminal plasma is the only parameter measured on day 0 to show a significant correlation with the percentage of viable spermatozoa after 3 days of semen storage (r = 0.495, P < 0.05). ROC analysis shows that TNF-α level is helpful in discriminating viability outcome after semen storage (AUC = 0.94, P < 0.001). We can predict with 92.35% certainty that fresh semen samples with more than 150 pg/ml of TNF-α in the seminal plasma will retain more than 85% of viable spermatozoa after 3 days of storage. Thus, TNF-α can contribute to predicting the quality of short-term stored semen.

scholarly journals A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

2016 ◽  
Vol 85 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Marina Anastasia Karageorgiou ◽  
Georgios Tsousis ◽  
Constantin M. Boscos ◽  
Eleni D. Tzika ◽  
Panagiotis D. Tassis ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Medium Term ◽  
Semen Collection ◽  
Chromatin Integrity ◽  
Boar Semen ◽  
Short Time

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

scholarly journals The Function of Casual Sex Action and Inaction Regret: A Longitudinal Investigation

2021 ◽  
Vol 19 (1) ◽  
pp. 147470492199833
Author(s):  
Leif Edward Ottesen Kennair ◽  
Trond Viggo Grøntvedt ◽  
Mons Bendixen
Keyword(s):  
Casual Sex ◽  
Adaptive Function ◽  
Short Term ◽  
Behavioral Choices ◽  
Sexual Disgust ◽  
Sexual Regret ◽  
Adaptive Shifts ◽  
Sexual Strategies Theory

In several recent papers the sex difference in regret predicted by sexual strategies theory has been supported: men more than women report regret passing up short-term sexual opportunities (inaction regret), while women regret having had sexual encounters (action regret). However, the adaptive function of regret, to improve future behavioral choices, has not been tested. In this first longitudinal test of behavioral change following regret, we consider whether regret actually results in adaptive shifts of behavior: will men who regret passing up sex engage in more short-term sex following regret? Will women who regret short-term encounters either choose better quality partners, reduce number of one-night stands or shift their strategy to long-term relationships? Across two waves (NT1 = 399, 65.4% women and NT2 = 222, 66.2% women) students responded to questions about casual sex action regret and inaction regret, along with possible outcomes, intrapersonal traits, and concurrent contextual predictors. There was no clear evidence for the proposed functional shifts in sexual behavior. Casual sex regret was associated with respondent sex and stable individual differences, such as sociosexual attitudes, regret processing and metacognitions, but the effect of these predictors were not consistent across the two waves. Among the tested concurrent contextual predictors, sexual disgust was the most consistent across waves. Regret is considered a gauge of the value and quality of the short-term sexual encounter. However, tentatively we conclude that after this first test of function using longitudinal data, we find no evidence of a mating strategy shifting effect following sexual regret.

scholarly journals Once-a-day Tadalafil administration improves the spermogram parameters in fertile patients

2015 ◽  
Vol 87 (3) ◽  
pp. 210 ◽  
Author(s):  
Antonio Corvasce ◽  
Giuseppe Albino ◽  
Teresa Leonetti ◽  
Agnese Filomena Buonomo ◽  
Ettore Cirillo Marucco
Keyword(s):  
Seminal Fluid ◽  
Semen Quality ◽  
Type A ◽  
Daily Treatment ◽  
Sperm Cells ◽  
Average Increase ◽  
Once Daily ◽  
Positive Effects ◽  
Semen Volume

Objectives: We explored the safety of Tadalafil once-daily treatment for 12 week and its effects on semen quality in a clinical subpopulation of men with psychogenic erectile dysfunction (ED). Patients and Methods: Twenty-seven men, between 19 and 35 years, unaware of their fertility status, suffering from psychogenic ED were enrolled. The examination of the seminal fluid was performed twice before administration of Tadalafil and twice after three months of continuous daily administration of Tadalafil 5 mg. The volume of the seminal fluid, the concentration of sperm, the concentration of sperm with type “a+b” motility, the concentration of sperm with type “a” motility, the concentration of normal sperm were taken into consideration. The results before treatment with Tadalafil (T0) and after 3 months of treatment (T3m) were compared. Results: The administration of once-daily Tadalafil 5mg, brings to an average increase of the total number of sperm cells, both total and fast motility (type a) and the percentage of nemasperms, and to an average increase of semen volume of only 0.41 ml. These quantitative and qualitative improvements of the seminal fluid resulted statistically significant as regard motility, nemasperm percentage and seminal fluid volume. No unespected safety findings were observed. Conclusions: Tadalafil administration improves the quality of sperm cells and seminal fluid: in particular motility, percentage of nemasperms and volume of seminal fluid. We emphasize the safety of the once-daily treatment with tadalafil 5mg and the positive effects on spermatogenesis.

scholarly journals ImmunoCAP® ISAC and Microtest for multiplex allergen testing in people with difficult to manage allergic disease: a systematic review and cost analysis

2016 ◽  
Vol 20 (67) ◽  
pp. 1-178 ◽  
Author(s):  
Marie Westwood ◽  
Bram Ramaekers ◽  
Shona Lang ◽  
Nigel Armstrong ◽  
Caro Noake ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Cost Effectiveness ◽  
Conference Proceedings ◽  
Citation Index ◽  
Clinical Effectiveness ◽  
Short Term ◽  
Cost Analyses ◽  
Immunocap Isac

BackgroundAllergy is a form of immune-mediated exaggerated sensitivity (hypersensitivity) to a substance that is either inhaled, swallowed, injected or comes into contact with the skin. Foreign substances that provoke allergies are called allergens. It has been claimed that multiplex allergen testing may help in diagnosing the cause of symptoms in patients with an unclear cause of allergy or who are allergic to more than one substance.ObjectivesTo evaluate multiplex allergen testing [devices that can measure the presence of multiple immunoglobulin E (IgE) antibodies in a patient’s blood at the same time], by assessing (1) clinical effectiveness (allergy symptoms, incidence of acute exacerbations, mortality, adverse events of testing and treatment, health-care presentations or admissions, health-related quality of life); (2) effects on treatment (diet, immunotherapy medications, other potential testing); (3) any additional diagnostic information provided by multiplex allergen testing; and (4) cost-effectiveness (cost of different assessment strategies).MethodsFifteen databases were searched from 2005 to April 2015, including MEDLINE (via OvidSp), MEDLINE In-Process Citations, MEDLINE Daily Update, PubMed (National Library of Medicine), EMBASE, Cochrane Database of Systematic Reviews (CDSR), Cochrane Central Register of Controlled Trials (CENTRAL), Database of Abstracts of Reviews of Effects (DARE), Health Technology Assessment (HTA) database, Science Citation Index (SCI), Conference Proceedings Citation Index-Science (CPCI-S), BIOSIS Previews, Latin American and Caribbean Health Sciences Literature (LILACS), National Institute for Health Research (NIHR) HTA programme, and the US Food and Drug Administration (FDA); supplementary searches of conference proceedings and trials registries were performed. Review methods followed published guidance from the Cochrane Collaboration and the Centre for Reviews and Dissemination, University of York, UK. The methodological quality of included studies was assessed using appropriate published tools or a review-specific tool designed by the project team. Studies were summarised in a narrative synthesis. Owing to a lack of data on the clinical effectiveness of multiplex allergen testing, no long-term cost-effectiveness model was developed. A conceptual model structure was developed and cost analyses were performed to examine the short-term costs of various possible diagnostic pathways.ResultsFifteen studies were included in the review. The very limited available data indicated that the addition of multiplex allergen testing [ImmunoCAP®Immuno Solid-phase Allergen Chip (ISAC), Thermo Fisher Scientific/Phadia AB, Uppsala, Sweden] to standard diagnostic work-up can change the clinicians’ views on the diagnosis, management and treatment of patients. There was some indication that the use of ImmunoCAP ISAC testing may be useful to guide decisions on the discontinuation of restrictive diets, the content of allergen-specific immunotherapy (SIT) prescriptions, and whether or not patients should receive SIT. However, none of the studies that we identified reported any information on clinical outcomes subsequent to changes in treatment or management. There was some evidence that ImmunoCAP ISAC may be useful for discriminating allergens that are structurally similar and are recognised by the same IgE antibody (cross-immunoreactive). No data were available for Microtest (Microtest Matrices Ltd, London, UK). Detailed cost analyses suggested that multiplex allergen testing would have to result in a substantial reduction of the proportions of patients receiving single IgE testing and oral food challenge tests in order to be cost-saving in the short term.ConclusionsNo recommendations for service provision can be made based on the analyses included in this report. It is suggested that a consensus-based protocol for the use of multiplex allergen testing be developed. The clinical effectiveness and cost-effectiveness of the proposed protocol should then be assessed by comparing long-term clinical and quality of life outcomes and resource use in patients managed using the protocol with those managed using a standard diagnostic pathway.Study registrationThis study is registered as PROSPERO CRD42015019739.FundingThis project was a Diagnostic Assessment Report commissioned by the NIHR HTA programme on behalf of the National Institute for Health and Care Excellence.

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

Comparison of Pedestrian Count Expansion Methods: Land Use Groups versus Empirical Clusters

2018 ◽  
Vol 2672 (43) ◽  
pp. 87-97 ◽  
Author(s):  
Julia B. Griswold ◽  
Aditya Medury ◽  
Robert J. Schneider ◽  
Offer Grembek
Keyword(s):  
Land Use ◽  
Count Data ◽  
Factor Group ◽  
Peak Activity ◽  
Short Term ◽  
Estimation Errors ◽  
Classification Approach ◽  
Volume Estimates

Expansion factors based on the trends in long-term count data are useful tools for estimating daily, weekly, or annual volumes from short-term counts, but it is unclear how to differentiate locations by activity pattern. This paper compares two approaches to developing factor groups for hour-to-week pedestrian count expansion factors. The land use (LU) classification approach assumes that surrounding LUs affect the pedestrian activity at a location, and it is easy to apply to short-term count locations based on identifiable attributes of the site. The empirical clustering (EC) approach uses statistical methods to match locations based on the actual counts, which may produce more accurate volume estimates, but presents a challenge for determining which factor group to apply to a location. We found that both the LU and EC approaches provided better weekly pedestrian volume estimates than the single factor approach of taking the average of all locations. Further, the differences between LU and EC estimation errors were modest, so it may be beneficial to use the intuitive and practical LU approach. LU groupings can also be modified with insights from the EC results, thus improving estimates while maintaining the ease of application. Ideal times for short-term counts are during peak activity periods, as they generally produce estimates with fewer errors than off-peak periods. Weekly volume estimated from longer-duration counts (e.g., 12 h) is generally more accurate than estimates from shorter-duration counts (e.g., 2 h). Practitioners can follow this guidance to improve the quality of weekly pedestrian volume estimates.

scholarly journals COVID-19 pandemic. Effects of stress

2020 ◽  
Vol 1 (2) ◽  
pp. 16-24
Author(s):  
Elena S. Akarachkova ◽  
◽  
Anton A. Beliaev ◽  
Dmitrii V. Blinov ◽  
Evgenii V. Bugorskii ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Health Care ◽  
Preventive Health Care ◽  
Preventive Health ◽  
World Health ◽  
Social Stability ◽  
Short Term ◽  
Health Organization

World Health Organization declared COVID-19 outbreak a pandemic on March 11, 2020. Fear of illness, self-isolation/quarantine, and reduced quality of life dramatically increased the prevalence of stress-related disorders in the population. Therefore, it is necessary to implement the preventive health-care measures aimed at short-term and long-term COVID-19 pandemic consequences reduction and promotion of social stability.

USE OF REGRESSION ANALYSIS IN FORECAST TASKS

2020 ◽  
pp. 22-27
Author(s):  
E.S. Lartseva ◽  
◽  
A.D. Kuznetsova
Keyword(s):  
Regression Analysis ◽  
Animal Population ◽  
Short Term ◽  
Statistical Estimates ◽  
Term Forecast ◽  
Applied Software ◽  
The Family ◽  
The Russian Federation

Based on official statistics on the number, of representatives of the family of non-ruminant cloven-hoofed animals (Artiodactyl) on the territory of the Russian Federation. Using the example of two species: domestic pigs and wild boars, the dynamics of the indicator for the long term is analyzed. Multidirectional trends were revealed for each species. Mathematical models of the dynamics of the livestock were obtained using the methods of regression analysis and applied software. Statistical estimates of the quality of animal population models were obtained. The short-term forecast for 2020 has been fulfilled.

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