Value of semen parameters, with special reference to TNF-α, in predicting the quality of boar semen after short-term storage

2013 ◽  
Vol 61 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Janko Mrkun ◽  
Marjan Kosec ◽  
Petra Zrimšek
Keyword(s):  
Semen Quality ◽  
Semen Parameters ◽  
Short Term ◽  
Progressive Motility ◽  
Tnf Α ◽  
Semen Storage ◽  
Boar Semen ◽  
Α Level ◽  
Term Storage

The aim of this study was to address the question whether changes in boar semen quality after short-term storage could be predicted on the basis of standard semen parameters and TNF-α level determined on the day of semen collection under commercial conditions. Progressive motility showed the highest positive correlation with morphology on day 0 of collection, and progressive motility on day 3 (P < 0.05) showed a negative correlation with acrosome abnormalities (P < 0.05). According to the area under receiver operating characteristics (ROC) curves (AUCs), progressive motility could also be used in predicting semen quality after 3 days of storage (AUC > 0.5; P < 0.05). TNF-α in seminal plasma is the only parameter measured on day 0 to show a significant correlation with the percentage of viable spermatozoa after 3 days of semen storage (r = 0.495, P < 0.05). ROC analysis shows that TNF-α level is helpful in discriminating viability outcome after semen storage (AUC = 0.94, P < 0.001). We can predict with 92.35% certainty that fresh semen samples with more than 150 pg/ml of TNF-α in the seminal plasma will retain more than 85% of viable spermatozoa after 3 days of storage. Thus, TNF-α can contribute to predicting the quality of short-term stored semen.

PSV-8 Effects of Air Exposure and Agitation on Quality of Stored Boar Semen Samples

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 210-211
Author(s):  
Kayode B Balogun ◽  
Kara R Stewart
Keyword(s):  
Bacterial Growth ◽  
Semen Quality ◽  
Short Term ◽  
Air Exposure ◽  
Storage Container ◽  
Semen Volume ◽  
Semen Storage ◽  
Boar Semen

Abstract Recent adoption of post cervical artificial insemination facilitates lowered concentrations of semen inseminated. In order to achieve this reduced concentration, the volume of the insemination dose has been reduced and replaced with additional air in the semen storage container. The objective of this study was to investigate the effects of semen volume, air contact inside semen dose tubes, daily agitation of semen doses and extender type on semen quality, thermo-resistance and bacterial growth in extended boar semen doses over 7 days of liquid storage at 17 C. Ejaculates from 4 terminal cross-bred boars were collected for 4 weeks and used in the 3 x 2 x 2 factorial study. The effects of treatment (CON: 80ml doses sealed at the top of the tube; 40HIGH: 40ml doses sealed at top of tube; and 40LOW: 40ml doses sealed at top of the liquid), extender type (long-term vs short-term), and agitation (agitated vs not agitated) were investigated. The result of the study revealed that motility (P=0.014) and viability (P=0.007) in 40HIGH were lower than CON. pH (P&lt; 0.001) was higher in 40HIGH compared to CON. Agitation did not impact motility (P=0.541), viability (P=0.406) or morphology (P=0.970) while long-term extender maintained higher motility (P=0.034), viability (P&lt; 0.001) and normal acrosomes (P&lt; 0.001) compared to short-term extender. VAP (P=0.039) of 40HIGH was lower than CON in a thermo-resistance test. Bacteria were cultured on both sheep’s blood and MacConkey agar and neither treatment (P=0.798; 0.766) nor agitation (P=0.396; 0.476) impacted bacterial growth in this study. In conclusion, semen doses prepared with 80mL or 40mL of total volume with minimal air contact in the tubes yield more desirable semen quality, as air contact negatively impacts boar semen pH and sperm motility. Additionally, regardless of volume or air exposure, daily agitation of boar semen doses did not affect semen quality.

PSV-9 Effects of Natural Honey Inclusion in Dilution and Freezing Extenders on Frozen-thawed Semen Quality in Boars

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 211-211
Author(s):  
Kayode B Balogun ◽  
Griffin Nicholls ◽  
Olujide Sokunbi ◽  
Kara R Stewart
Keyword(s):  
Semen Quality ◽  
Swine Industry ◽  
Normal Cells ◽  
Progressive Motility ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Boar Semen ◽  
Semen Extender ◽  
Natural Honey

Abstract Improvements in the post-thaw quality of frozen semen could allow increased utilization of this technology in the swine industry. The objective of this study was to investigate the effects of natural honey inclusion in semen extender and freezing media on motility, mobility, and morphology of cryopreserved boar sperm. Ejaculates from 6 terminal cross-bred boars were collected using the gloved-hand technique for 3 weeks and used in a 2 x 3 factorial study design. Following collection, semen samples were incubated overnight in dilution extender with and without natural honey (D0: Androhep Plus; D1: Androhep Plus + 0.25% honey). The following day, the semen samples were cooled to 4 C in LEY cooling extender then frozen in freezing media containing 93% cooling extender + 6% glycerol + 1% Equex-STM Paste (F1), or freezing media with natural honey replacing 50% of the glycerol (F2) or 50% of the Equex-STM paste (F3). Semen samples were frozen using a controlled-rate freezer and stored in liquid nitrogen. Two straws per treatment for each boar were thawed and semen quality assessed. The inclusion of natural honey in dilution extender had no effect on post-thaw motility (P=0.733), progressive motility (P=0.562), or other mobility parameters (0.995≤P≥0.081). However, D1 had a higher percentage of normal acrosomes (P=0.001) and morphologically normal cells (P&lt; 0.001) resulting from lower tail abnormalities compared to D0 (P=0.006). Post-thaw motility (P&lt; 0.001) and progressive motility (P&lt; 0.001) were increased in F3 compared to both F2 and F1. F1 had reduced normal acrosomes (P=0.009) and morphologically normal cells (P&lt; 0.001) resulting from higher tail abnormalities (P&lt; 0.001). In conclusion, the inclusion of natural honey, at 0.25%, in dilution extender helps maintain normal sperm and acrosome morphology, and replacing 50% Equex-STM Paste with honey in freezing extender improves post-thaw sperm motility and progressive motility of frozen-thawed boar semen.

scholarly journals Liquid Storage of Boar Semen Using Commercial Extenders

2018 ◽  
Vol 75 (1) ◽  
pp. 66
Author(s):  
Liviu BOGDAN ◽  
Mihai CENARIU ◽  
Mihai BORZAN ◽  
Simona CIUPE ◽  
Lehel SZABO ◽  
...  
Keyword(s):  
Semen Parameters ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Before And After ◽  
Boar Semen ◽  
Casa System ◽  
Sexually Mature ◽  
Term Storage

The research was focused on the modern evaluation of boar semen parameters, using computer assisted sperm analysis (CASA), before and after liquid storage at 15ºC. Semen was collected from 15 sexually mature boars by manual stimulation. Macroscopical and microscopical evaluation of semen was performed, followed by a detailed CASA analysis of all ejaculates. Subsequently, semen was diluted using 4 different extenders (Semtest, Androstar, MIII and Cronos) and stored at 15ºC for 24 hours. Next, evaluation of progressive motility, total motility and viability was performed, using the same CASA system. All experiments were performed in triplicates and results were statistically analyzed. The average progressive motility after 24 hours was as follows: 69.56 ± 6.38 for MIII, 65.92% ± 2.63 for Semtest, 67.07% ± 5.58 for Androstar Plus and 68.93% ± 3.40 for Cronos. The viability results after 24 hours were: 86.34% ± 1.38 for Semtest extender, 93.55% ± 3.38% for Androstrar Plus, 89.19% ± 3.42 for MIII and 91.35% ± 2.37 for Cronos. The findings of this study suggest that the use of commercial extenders for short-term storage of swine semen is important in order to increase sperm longevity with minimal sperm function deterioration.

scholarly journals Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

2019 ◽  
Vol 71 (2) ◽  
pp. 489-499
Author(s):  
B.A. Pereira ◽  
L.G.P. Rocha ◽  
M.C. Teles ◽  
W.E. Silva ◽  
J.A. Barbosa ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Sperm Motility ◽  
Semen Quality ◽  
Semen Parameters ◽  
Mitochondrial Activity ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Acrosomal Integrity

ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

scholarly journals The Influence of Air Contact on the Quality of Extended Boar Semen

2018 ◽  
Vol 44 (1) ◽  
pp. 7
Author(s):  
Caroline De Veronez Ribeiro ◽  
Júlia Linck Moroni ◽  
Mariana Boscato Menegat ◽  
Ana Paula Gonçalves Mellagi ◽  
Mari Lourdes Bernardi ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Repeated Measures ◽  
Semen Quality ◽  
Random Effect ◽  
Semen Parameters ◽  
Swine Industry ◽  
Subsequent Decrease ◽  
Boar Semen

Background: The extensive use of artificial insemination in the swine industry emphasizes the need for distribution of good quality semen doses by the artificial insemination centers. A factor that could modify seminal quality is the contact of extended semen with environmental air. However, only few studies have investigated the effects of air presence on semen parameters. Thus, the aim of the present study was to evaluate the influence of different amounts of air on porcine semen quality during the storage period.Materials, Methods & Results: Four ejaculates from five sexually mature boars were used. Each ejaculate (n = 20) was isothermically diluted (30 x 106/mL) in a BTS-extender and assigned to three groups differing on the amount of air entrapped within the polystyrene tubes (100 mL). In group AIR-0 the tubes were completely filled with extended semen (0% air); in group AIR-25, 75% of the tube volume was filled with extended semen and 25% with air; in group AIR-50, 50% of the tube volume was filled with extended semen. The semen doses were stored at 17.3 ± 0.5ºC for 120 h. The motility parameters were assessed at 24, 72 and 120 h of storage using a CASA system. The evaluation of acrosomal integrity was performed by examining formalin-fixed samples under phase-contrast microscopy (1000 x) at 72 and 120 h of storage. The pH was measured at 24, 48, 72, and 120 h of storage with a digital pH-meter. At 120 h, samples were incubated in waterbath at 38°C for the thermoresistance test and sperm motility was assessed after 30 and 120 min of incubation. For each time analysis, a different sealed tube was used in order to maintain a constant amount of air within the tubes during storage. Data were analyzed as repeated measures using the GLIMMIX procedure of SAS® Software. Boar and week of collection were included as random effect in the model. Groups were compared using the Tukey-Kramer test at a significance level of 5%. The pH was significantly influenced by the amount of air entrapped within the tubes (P < 0.01). Throughout the experiment, the pH of semen doses was higher as the amount of air increased within the tubes. The mean pH for groups AIR-50, AIR-25 and AIR-0 was 7.51 ± 0.06, 7.25 ± 0.06 and 7.07 ± 0.06, respectively. Overall motility was similar among the groups for up to 24 h of storage. At 72 h and 120 h of storage, total, progressive and rapid motility were higher (P < 0.05) in AIR-0 compared to AIR-50. The kinematic parameters DAP and VAP decreased as the volume of air within the tubes increased (P < 0.05). Group AIR-0 exhibited higher values (P < 0.05) for DSL, DCL, VSL, VCL, BCF, and WOB compared to AIR-25 and AIR-50. Furthermore, after the thermoresistance test performed at 120 h of storage, total motility, progressive motility and rapid motility were negatively affected (P < 0.05) by the increasing amounts of air within the tubes. There was no effect of air amount within the semen doses on acrosome integrity (P > 0.05).Discussion: The presence of air within tubes affects the quality of extended semen during storage, resulting in increase of pH and subsequent decrease in motility preservation. This pH-rise could be explained by the loss of CO2 from the diluted semen to the air compartment within the tube. At the same time, it is possible to infer that this event influences the intracellular pH of the spermatozoa, increasing sperm metabolism and, consequently, impairing sperm motility. Therefore, we conclude that avoiding air presence within storage containers is recommended in order to ensure the quality of boar semen doses.

scholarly journals Study to evaluate Association of Tobacco Chewing and Smoking with Semen Parameters

2017 ◽  
Vol 8 (3) ◽  
pp. 97-100
Author(s):  
Neeraj Aggarwal ◽  
Rishi R Vohra ◽  
Shivam Priyadarshi ◽  
Nachiket Vyas ◽  
Sher S Yadav ◽  
...  
Keyword(s):  
Sperm Morphology ◽  
Semen Quality ◽  
Semen Parameters ◽  
Age Group ◽  
Chewing Tobacco ◽  
Progressive Motility ◽  
Fertility Status ◽  
Tobacco Abuse ◽  
Tobacco Chewing

ABSTRACT Introduction Many studies have shown deleterious effects of tobacco abuse in any form on semen quality. We studied the association of tobacco chewing, smoking, and their combination on semen characteristics. Materials and methods Our study was performed on 216 normal asymptomatic healthy males (49 controls, 54 smokers, 55 tobacco chewers, and 58 consuming both) in the age group of 24 to 35 years. The effect on semen parameters was analyzed. Results Consuming both forms of tobacco individually and in combination had statistically significant effect on sperm morphology, progressive motility, and semen concentration. Other parameters show nonstatistically significant decline compared with controls. Conclusion As smoking and chewing tobacco negatively affect quality of semen, strategies should be developed to direct attention of the general population toward its effect on fertility status of male. How to cite this article Vohra RR, Priyadarshi S, Aggarwal N, Vyas N, Yadav SS, Tomar V. Study to evaluate Association of Tobacco Chewing and Smoking with Semen Parameters. Int J Infertil Fetal Med 2017;8(3):97-100.

scholarly journals Assessment of quality in specific fractions of Large White Yorkshire boar semen

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  
Keyword(s):  
Cold Shock ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Membrane Cholesterol ◽  
Large White ◽  
Progressive Motility ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Quality Assessments

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

COMPARATIVE CHARACTERISTICS OF RAM SEMEN FROZEN IN DIFFERENT EXTENDERS

2021 ◽  
pp. 63-78
Author(s):  
M.M. AYBAZOV ◽  
◽  
A.N. SHEVCHENKO ◽  
M.I. SELIONOVA ◽  
T.V. MAMONTOVA
Keyword(s):  
Egg Yolk ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Progressive Motility ◽  
Kinematic Performance ◽  
Synthetic Media ◽  
Ram Sperm

Numerous studies have proved the necessity of egg yolk in synthetic media to dilute semen before its cryopreservation. However, at the same time, it has been demonstrated that its use can adversely affect the quality of frozen-thawed sperm. The present study compares the main quality parameters of ram sperm frozen using TRIS-based diluent with egg yolk and two egg yolk-free diluents (OvixCell® and AndroMed®). A slower deterioration in the kinematic performance of sperm cryopreserved in TRIS diluent with native egg yolk confirmed higher cryoprotective performance compared to commercial extenders containing no egg yolk. Significantly higher total and progressive motility was observed in TRIS-based medium with egg yolk (P< 0.05). This advantage was maintained after four hours of incubation and became more significant at the end of cultivation (after six hours) (P< 0.01). Thus, ram sperm frozen in egg yolk medium retained better motility than in egg yolk-free extenders, which allows predicting its higher bioavailability. Assessment of some semen parameters using CASA showed that there were no significant differences in motility between the three extenders immediately after thawing the straws. When assessed two hours after thawing, a diluent containing egg yolk (TRIS-based) was found to have higher results for some of the examined traits than phospholipid diluents.

scholarly journals 284 Gene polymorphism of 5, 10-methylene tetrahydrofolate reductase is related to boar semen quality

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 117-117
Author(s):  
wen lai ◽  
chao Wang ◽  
Jian Peng
Keyword(s):  
Semen Quality ◽  
Mthfr Gene ◽  
Missense Mutations ◽  
Double Mutation ◽  
Polymorphic Loci ◽  
Synonymous Mutations ◽  
Methylene Tetrahydrofolate Reductase ◽  
Boar Semen ◽  
Methylene Tetrahydrofolate

Abstract 5, 10-methylene tetrahydrofolate reductase (MTHFR) is an important enzyme in folate and homocysteine metabolism, and plays an important role in regulating folate levels, DNA synthesis and methylation in cells. Defects in the MTHFR gene can lead to spermatogenesis disorders and male sterility. Polymorphic loci of MTHFR gene and semen quality of 1,490 boars were examined to explore their relationship. Results showed there were 13 polymorphic loci on MTHFR gene exon in boars with different semen quality, among which loci 1 and 2 were missense mutations. For Duroc boars, although there were synonymous mutations at loci 3–5 and 11, the mutation had no effect on semen quality (P &gt; 0.05). In addition to loci 13, loci 1–12 of the MTHFR gene had mutations, but it had no effect on semen quality in Yorkshire boars (P &gt; 0.05). Interestingly, for Landrace boars, double mutation of MTHFR gene at 1–2, 6–10, and 12 loci (CC → TT) led to decreased sperm motility and increased abnormal sperm rate (P &lt; 0.05). Further analysis showed seminal plasma MDA and hydrogen peroxide levels were increased in Landrace boars with the mutation of MTHFR gene at loci 1–2, 6–10 and 12 (P &lt; 0.05). In conclusion, double mutation of MTHFR gene at 1–2, 6–10, and 12 loci decreased semen quality and increased oxidative stress in sperm, and these mutant loci may be potential biomarkers for predicting the semen quality of Landrace boars.

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