Determination of Nutrient Digestibility in Calves Supplemented with Various Levels of Corn Gluten Feed When Consuming Forage-based Rations

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 33-33
Author(s):  
Charles A Zumbaugh ◽  
Emily N Nolen ◽  
Thomas B Wilson

Abstract The experimental objective was to determine the effect of supplementing various levels of corn or corn gluten feed (CGF) on apparent total tract nutrient digestibility in cattle consuming a forage-based ration. Twelve purebred Angus and Simmental heifers were stratified across treatments by BW and breed in a 2x2 factorial arrangement across 2 periods (n = 6/treatment). Treatments included either corn or CGF supplemented at 0.25% BW or 0.75% BW with ad libitum access to alfalfa hay. Each period lasted 21 d, with animals housed as a group and moved to individual stalls to consume supplement each morning during the first 10 d and individually thereafter. Fresh alfalfa and supplement were provided daily throughout the experiment, and feed offered and refused were recorded daily while animals were housed individually. During the final 3 d of each period, fecal samples were obtained directly from the rectum of each animal every 6-h. Samples were analyzed for neutral detergent fiber (NDF), organic matter (OM), and crude protein (CP). Data were analyzed using lm in R with significance defined at P ≤ 0.05. The statistical model included the fixed effects of supplement type, level, and their interaction, breed, and period. No differences (P ≥ 0.28) in ADG or G:F were noted among treatments. Total tract NDF, ADF, and OM digestibility did not differ among treatments, but OM and ADF digestibility tended to be greater (P = 0.10 and P = 0.06, respectively) for animals supplemented with corn. Crude protein digestibility was not influenced by supplement type (P = 0.46), but tended to be greater (P = 0.07) for animals supplemented at 0.25% BW. These results indicate that at supplement levels below 0.75% BW, supplementing cattle with CGF does not result in an improvement in apparent total tract digestibility relative to corn under the conditions of this experiment.

2020 ◽  
Vol 19 (1) ◽  
pp. 1264-1274
Author(s):  
Babak Darabighane ◽  
Farzad Mirzaei Aghjehgheshlagh ◽  
Ali Mahdavi ◽  
Bahman Navidshad ◽  
John K. Bernard

2014 ◽  
Vol 83 (3) ◽  
pp. 225-231
Author(s):  
Iveta Maskaľová ◽  
Vladimír Vajda ◽  
Marek Krempaský ◽  
Lukáš Bujňák

Knowledge of the profile of amino acids of the rumen-undegradable protein can help in the formulation of diets to provide amino acids that complement microbial protein as well as supply amino acids, which are most limiting for milk production. Three non-lactating cows fitted with rumen cannulas were used to determine the effect ofin siturumen degradation on crude protein and amino acid profile of rumen-undegraded protein of feedstuffs. The obtained values of rumen degradability of crude protein with significant difference (P< 0.001) between feeds ranged from 20.3 to 76.3% (mean 62.0 ± 17.9%) and values of total amino acids ranged from 30.9% in corn gluten meal to 83.8% in corn gluten feed (mean 67.5 ± 16.4%). Anin vitromodified 3-step method was used to determine intestinal digestibility. Intestinal digestibility of undegraded protein varied from 54.5 ± 1.4% in raw soybean to 95.2 ± 1.0% in corn gluten feed. The absorbable amino acid profile of rumen-undegraded protein for each feedstuff was compared with profiles of the original feedstuff and the rumen-exposed undegraded protein. Absorbable lysine (9.3 ± 1.1 g/kg of crude protein) was higher in products of soybean and sunflower cake. Corn gluten feed and meal supplied more absorbable methionine (3.6 ± 0.6 g/kg of crude protein). This study showed that the digestibility factor of crude protein and amino acid based onin situandin vitromethods for thermal treatment of protein feeds can be used in models to optimize the amino acid nutrition of dairy cows and expand knowledge about rumen degradability and ileal digestibility of amino acids in feedstuffs.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 149-150
Author(s):  
Mariana E Garcia-Ascolani ◽  
Nicolas DiLorenzo ◽  
Yan Sun ◽  
Thierry Aubert ◽  
Haley Larson

Abstract Twelve dual-flow continuous culture fermenters (1.95 L) were used to evaluate the effect of a phytogenic feed additive (Actifor Pro, Delacon, Engerwitzdorf, Austria; ACT) on ruminal fermentation and methane production when fed high fiber diets, comprised of bermudagrass hay (BGH) with or without corn gluten feed (CGF). Fermenters were utilized in a generalized randomized block design with a 2 × 2 factorial arrangement of treatments (n = 6): 1) diet (with or without CGF at 22% of diet DM) and 2) additive (with or without ACT at 1.0 g/L ACT). Two 10-d periods were conducted. Overall, comparing to no CGF, supplementation with CGF resulted in lower dry matter, organic matter, crude protein digestibility, microbial efficiency of nitrogen utilization, and methane production (mL of CH4/mol of total VFA), but increased neutral detergent fiber digestibility (all P ≤ 0.05). Diet × additive interactions were observed for molar proportion of acetate and propionate, and acetate-to-propionate ratio (A:P, all interactions P &lt; 0.05), where inclusion of ACT increased acetate molar proportion and A:P (both P &lt; 0.05), and decreased propionate molar proportion in diets with CGF (P = 0.05). A diet × additive interaction was also observed for methane production (ml of CH4/mol of total VFA; P = 0.08), where ACT decreased CH4 production per mol of VFA by 42% (P = 0.04), when only BGH was fed to the fermenters. In conclusion, CGF supplementation in BGH diets reduced methane production and improved fiber digestibility. The decrease in methane production per mol of VFA observed with ACT in the BGH without CGF diet warrants further investigation.


2019 ◽  
Vol 43 (4) ◽  
Author(s):  
Rahma Fitriastuti ◽  
Lies Mira Yusiati ◽  
Budi Prasetyo Widyobroto ◽  
Zaenal Bachruddin ◽  
Chusnul Hanim

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.


1997 ◽  
Vol 13 (1) ◽  
pp. 24-29
Author(s):  
J.K. Bernard ◽  
J.D. Quigley ◽  
H.H. Dowlen ◽  
K.C. Lamar ◽  
D. Johnson

1991 ◽  
Vol 74 (11) ◽  
pp. 3913-3919 ◽  
Author(s):  
J.K. Bernard ◽  
R.C. Delost ◽  
F.J. Mueller ◽  
J.K. Miller ◽  
W.M. Miller

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 400-400
Author(s):  
Gleise M Silva ◽  
Federico Podversich ◽  
Ana C G Luna ◽  
Gonzalo Barreneche ◽  
Nicolas DiLorenzo

Abstract The integrity of a diet fed as a total mixed ration (TMR) in feedlot and backgrounding scenarios is essential to maintain animal performance and minimized digestive upsets. The objective of this study was to quantify crude protein (CP), dry matter (DM) and ash concentration of a backgrounding diet fed as a TMR in a GrowSafe feed intake monitoring system. Angus crossbreed heifers (n = 70; 360 ± 60 kg of BW; 470 ± 26 d of age) and steers (n = 20; 386 ± 65 kg of BW; 465 ± 30 d of age) were allocated to 18 concrete-floored pens (108 m2), equipped with two GrowSafe feed bunks each to receive a backgrounding diet fed ad libitum as a TMR. Diet was comprised of (DM basis): 35% cottonseed hulls, 34% cracked corn grain, 20% pelleted corn gluten feed, 5% cottonseed meal, 4% liquid supplement (molasses-based), and 2% limestone. Feed samples were collected from three segments of each bunk (top; TOP, middle; MID, and bottom; BOT).Samples from the TOP was collected immediately after feed was provided and further samplings were conducted when feed was consumed throughout the day to obtain the MID and BOT samples in a 24 h interval. Approximately 90 kg (as fed) of the TMR was provided at 0800 h in each clean feed bunk. Concentration of CP did not differ (P = 0.89) throughout the day. However, DM content decreased as feed was consumed (P &lt; 0.001). Concentration of ash was greater for the MID and BOT portions compared to TOP (P = 0.04). In conclusion, CP concentrations in the backgrounding diet fed were not altered throughout the day as diet was consumed. Increased ash concentration towards the bottom of the feed bunk prior to the new feed delivery may result from vertical stratification of the TMR.


1969 ◽  
Vol 92 (1-2) ◽  
pp. 27-38
Author(s):  
Héctor L. Díaz-Ríos ◽  
Abner A. Rodríguez-Carias

Two experiments were conducted to determine the effect of supplementation with tilapia fish silage (FS) prepared in laboratory scale silos with addition of cane molasses, on intake and digestibility of tropical grass hay (GH) (Digitaria eriantha, 80%, and Urochloa maximum, 20%), and rhizome perennial peanut hay (RPPH) (Arachis glabrata). In Experiment I, GH was the basal diet and treatments were Control or T1 = GH, and GH supplemented daily with FS at 0.45% (T2) and 0.90% (T3) of the animal body weight (BW). In Experiment II, the basal diet was RPPH and treatments were Control or T1 = RPPH;T2 = RPPH supplemented daily with FS at 0.225% BW; and T3 = RPPH with 0.45% BW. In both experiments nine adult intact rams were used. The animals were fed for eight days of adaptation and six days of data collection in each period. Both experiments used a 3 X 3 Latin Square design. In experiment I dry matter (DM) intake and digestibility of GH increased (P less than 0.05) with FS supplementation, which also increased crude protein (CP) intake. CP digestibility increased (P < 0.05) at each level of supplementation compared to that of the control, but T2 and T3 did not differ significantly. Neutral detergent fiber (NDF) intake was higher (P < 0.05) in T1 than in T3 but similar in T1 and T2. Fiber digestibility was not affected (P greater than 0.05) by the treatments. It was concluded that supplementation of a GH basal diet with FS at 0.90% of BW is the best alternative. In Experiment II, DM digestibility of T3 differed (P less than 0.05) from that in T1 but in T2 did not differ from DM digestibility either in T1 or T3. Intake of DM, CP and fiber were similar in all treatments (P greater than 0.05). Crude protein digestibility was similar at both levels of supplementation and superior (P < 0.05) to that of the control, but fiber digestibility was not affected (P > 0.05). It is concluded that daily supplementation with FS for sheep fed a basal diet of RPPH should be limited to 0.225% of BW. Upon comparing both experiments, voluntary intake of DM, NDF and CP from RPPH was higher than from GH.The legume also showed a higher (P < 0.05) digestibility of DM and CP.


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