scholarly journals Rumen degradability and ileal digestibility of proteins and amino acids of feedstuffs for cows

2014 ◽  
Vol 83 (3) ◽  
pp. 225-231
Author(s):  
Iveta Maskaľová ◽  
Vladimír Vajda ◽  
Marek Krempaský ◽  
Lukáš Bujňák
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Amino Acid Profile ◽  
Ileal Digestibility ◽  
Corn Gluten ◽  
Corn Gluten Feed ◽  
Gluten Feed

Knowledge of the profile of amino acids of the rumen-undegradable protein can help in the formulation of diets to provide amino acids that complement microbial protein as well as supply amino acids, which are most limiting for milk production. Three non-lactating cows fitted with rumen cannulas were used to determine the effect ofin siturumen degradation on crude protein and amino acid profile of rumen-undegraded protein of feedstuffs. The obtained values of rumen degradability of crude protein with significant difference (P< 0.001) between feeds ranged from 20.3 to 76.3% (mean 62.0 ± 17.9%) and values of total amino acids ranged from 30.9% in corn gluten meal to 83.8% in corn gluten feed (mean 67.5 ± 16.4%). Anin vitromodified 3-step method was used to determine intestinal digestibility. Intestinal digestibility of undegraded protein varied from 54.5 ± 1.4% in raw soybean to 95.2 ± 1.0% in corn gluten feed. The absorbable amino acid profile of rumen-undegraded protein for each feedstuff was compared with profiles of the original feedstuff and the rumen-exposed undegraded protein. Absorbable lysine (9.3 ± 1.1 g/kg of crude protein) was higher in products of soybean and sunflower cake. Corn gluten feed and meal supplied more absorbable methionine (3.6 ± 0.6 g/kg of crude protein). This study showed that the digestibility factor of crude protein and amino acid based onin situandin vitromethods for thermal treatment of protein feeds can be used in models to optimize the amino acid nutrition of dairy cows and expand knowledge about rumen degradability and ileal digestibility of amino acids in feedstuffs.

scholarly journals Standardized ileal digestibility of amino acids in cereal grains and co-products in growing pigs

2020 ◽  
Vol 33 (7) ◽  
pp. 1148-1155
Author(s):  
Su A Lee ◽  
Jong Young Ahn ◽  
Ah Reum Son ◽  
Beob Gyun Kim
Keyword(s):  
Amino Acids ◽  
Rice Bran ◽  
Wheat Bran ◽  
Soybean Meal ◽  
Cereal Grains ◽  
Corn Gluten Meal ◽  
Ileal Digestibility ◽  
Corn Gluten ◽  
Corn Gluten Feed ◽  
Gluten Feed

Objective: The objective was to determine standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in cereal grains and various co-products fed to growing pigs.Methods: Ten feed ingredients tested were barley (9.3% CP), lupin kernels (31.1% CP), and wheat (11.3% CP) as cereal grains, and 2 sources of corn gluten feed produced in China (21.6% CP) and Korea (24.6% CP), corn gluten meal (65.3% CP), lupin hulls (11.6% CP), rice bran (14.5% CP), soybean meal (44.8% CP), and wheat bran (15.4% CP) as co-products. Ten experimental diets were formulated to contain each ingredient as a sole source of N and an N-free diet was used to correct basal endogenous losses of CP and AA. All diets also contained 0.5% Cr2O3 as an indigestible index. A replicated 11×6 incomplete Latin square design with 11 dietary treatments, 6 periods, and 22 animals was employed. Twenty-two barrows with an initial body weight of 64.6±4.9 kg were equipped with a T-cannula in the distal ileum. An experimental period consisted of a 4-d adaptation period and a 2-d collection period.Results: The SID of CP in the barley, lupin kernels, wheat, 2 sources of corn gluten feed, corn gluten meal, lupin hulls, rice bran, soybean meal, and wheat bran were 84.7%, 90.5%, 90.4%, 77.4%, 74.6%, 89.5%, 90.4%, 74.4%, 86.9%, and 63.4% (standard error of the mean [SEM] = 5.3, p = 0.006), respectively. The respective SID values of Lys were 75.5%, 88.4%, 83.9%, 74.7%, 62.4%, 80.3%, 83.9%, 78.5%, 88.0%, and 71.2% (SEM = 3.3, p<0.001), and the SID values of Met were 83.6%, 88.7%, 89.4%, 85.7%, 78.3%, 88.9%, 89.4%, 85.3%, 91.1%, and 77.0% (SEM = 2.4, p<0.001), respectively.Conclusion: The ileal digestibility of protein and amino acids varies among the feed ingredients fed to pigs.

Ileal digestibility of amino acids in pigs given a barley-based diet with increasing inclusion of lucerne leaf meal

1998 ◽  
Vol 67 (1) ◽  
pp. 131-138 ◽  
Author(s):  
M. Reverter ◽  
J. E. Lindberg
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Protein Intake ◽  
Crude Protein ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Leaf Meal

AbstractIleal digestibility of amino acids in barley-based diets with increasing inclusion of lucerne leaf meal (LLM; 0, 50, 100 and 200 g/kg) was studied in a change-over experiment with post-valve T-caecum cannulated growing pigs. The apparent Heal digestibility of crude protein, as well as of most of the essential (EAA) and non-essential amino acids (NEAA) was not significantly affected by the dietary inclusion of LLM. The exceptions were cystine, methionine, phenylalanine, glutamic acid and serine, all of which showed a significant (P< 0·05) reduction in apparent Heal digestibility with increasing inclusion of LLM. In contrast, the calculated true Heal digestibility of all EAA (with the exception of arginine and lysine) and the NEAA glutamic acid and serine were significantly (P< 0·05) reduced with the inclusion of LLM. Associated with an increased crude protein intake, there was a significant (P< 0·05) increase in the amount of absorbed EAA when the proportion of LLM was increased in the diet. With increasing proportion of fibre in the diet, there was a significant (P< 0·05) increase in the Heal flow of glucosamine.It can be concluded from the present data that the inclusion of LLM in a barley-based diet for growing pigs will result in an improvement in the absorbed amino-acid profile due to a significant increase in the absorption of all of the EAA.

Amino acid digestibility of meat and bone meals for broiler chickens

2002 ◽  
Vol 53 (11) ◽  
pp. 1257 ◽  
Author(s):  
V. Ravindran ◽  
W. H. Hendriks ◽  
B. J. Camden ◽  
D. V. Thomas ◽  
P. C. H. Morel ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Ash Content ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Vitro Assay ◽  
Ileal Digestibility ◽  
Gross Energy

Variation in the apparent ileal digestibility of amino acids in 19 meat and bone meal samples, obtained from commercial rendering plants in New Zealand, was determined using 5-week-old broilers. Assay diets contained meat and bone meal as the only source of protein, and chromic oxide as an indigestible marker for the calculation of amino acid digestibility values. Correlations of chemical composition (crude protein, ash, crude fat, and gross energy) and in vitro assays (protein solubility in 0.2% potassium hydroxide and nitrogen digestibility by 0.2% pepsin hydrolysis) with ileal amino acid digestibility were also examined. Considerable variation was observed in the contents of crude protein (38.5–67.2 g/100 g), ash (13.0– 56.5 g/100 g), crude fat (4.3–15.3 g/ 100�g), and gross energy (9.4–22.3 MJ/kg) of meat and bone meal samples. The amino acid concentrations and ileal digestibility of amino acids also varied substantially. Cystine, the first limiting amino acid in meat and bone meal, had the lowest digestibility estimates. Correlation analyses showed that the ash content was the only chemical parameter that was consistently correlated with amino acid digestibility. Digestibility of amino acids, with the exception of aspartic acid, threonine, serine, tyrosine, histidine, and cystine, was negatively correlated with ash content, with samples with high ash levels having lower digestibility. Both in vitro assay measurements were found to be insensitive indicators of variations in amino acid digestibility.

scholarly journals Apparent ileal digestibility of amino acids and metabolisable energy value in grains for broilers

2008 ◽  
Vol 14 (4) ◽  
pp. 325 ◽  
Author(s):  
S. PERTTILÄ ◽  
J. VALAJA ◽  
T. JALAVA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Dry Matter ◽  
Nitrogen Excretion ◽  
Ileal Digestibility ◽  
Metabolisable Energy ◽  
Feed Protein ◽  
Digestible Amino Acids

Using ileal digestible amino acids in feed optimising will intensify feed protein utilizing and decrease nitrogen excretion to the environment. The study determined the apparent ileal digestibility (AID) coefficients of amino acids in barley, wheat, oats, triticale, maize, and dehulled oats in the diets of 180 Ross broiler chickens (aged 24–35 days). The birds were fed semi-purified diets that contained grain as the sole protein source and chromium-mordanted straw as an indigestible marker. The AID coefficients of the nutrients were assessed using the slaughter technique, and the apparent metabolisable energy (AME) was determined using total excreta collection. The ileal digestibility of the dry matter and organic matter were the highest in maize. The AME of maize was higher than that of other cereals. The ileal digestibility of crude protein was higher in wheat than that in barley, oats and dehulled oats. The AME of wheat was similar to that of barley and oats but lower than that of triticale and dehulled oats. The amino acid AID was highest in wheat (0.86) and triticale (0.85) and lowest in oats (0.79) and barley 0.77). The average amino acid AID was 0.81 in dehulled oats. The threonine AID was the same in all tested ingredients. The lysine, methionine, and cystine AID coefficients were 0.81, 0.79, and 0.71 respectively for barley; 0.86, 0.84, and 0.38 respectively for oats; 0.87, 0.86, and 0.53 respectively for dehulled oats; 0.84, 0.90, and 0.66 respectively for maize; 0.89, 0.88, and 0.77 respectively for triticale; and 0.87, 0.85, and 0.71 respectively for wheat. Results indicated that AME –values of domestic grains (barley, oats and wheat) are in the same level. Especially, low AME value of wheat needs further investigation.;

scholarly journals 131 MODIFICATION OF AMINO ACID CONCENTRATIONS IN MEDIUM BY PIG EMBRYOS FROM THE ZYGOTE TO THE BLASTOCYST STAGE

2005 ◽  
Vol 17 (2) ◽  
pp. 216
Author(s):  
P. Booth ◽  
T. Watson ◽  
H. Leese
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Embryonic Development ◽  
Amino Acid Profile ◽  
Blastocyst Stage ◽  
Morula Stage ◽  
Amino Acid Profiles ◽  
The Difference ◽  
Amino Acid Concentrations

Pre-implantation embryos can produce and consume amino acids in a manner dependent upon stage of embryonic development (Partridge and Leese 1996 Reprod. Fert. Dev. 8, 945) that may also be predictive of subsequent viability (Houghton et al. 2002 Hum. Reprod. 17, 999). To examine these relationships in the pig, the appearance or depletion of 18 amino acids from a presumptive near-physiological mixture was determined by HPLC in porcine in vitro-produced embryos from the zygote to the blastocyst stage. Cumulus oocyte complexes derived from slaughterhouse prepubertal pig ovaries were matured for 40 h in modified TCM-199 before being fertilized (Day 0) with frozen thawed semen in tris-based medium. After 6 h, presumptive zygotes were denuded and cultured in groups of 20 in NCSU medium modified to contain a physiological mixture of 18 amino acids including 0.1 mM glutamine (NCSUaa). Groups of 2–10 embryos (dependent on stage) were removed on Day 0 (1 cell), Day 1 (2- and 4-cell), Day 4 (compact morula), and Day 6 (blastocyst) and placed in 4 μL NCSUaa for 24 h. After incubation, the embryos were removed and the medium analyzed by HPLC. Each stage was replicated 3–9 times. Since amino acid profiles of 2- and 4-cell embryos were not different, data were combined. Overall, arginine (1.19 ± 0.33), glutamine (0.78 ± 0.34) and threonine (0.05 ± 0.04) were significantly (P < 0.01) depleted from the medium whereas alanine (0.21 ± 0.1), glycine (0.20 ± 0.06), asparagine (0.13 ± 0.5), lysine (0.1 ± 0.03), isoleucine (0.08 ± 0.01), valine (0.05 ± 0.01), leucine (0.04 ± 0.02), phenylalanine (0.03 ± 0.01), and histidine (0.02 ± 0.04) significantly (P < 0.05) accumulated (mean of the 4 sampling timepoints; all values pmol/embryo/h ± SEM). The difference between amino acid accumulation and depletion (balance) was approximately equivalent between Day 0 and the morula stage although turnover (sum of depletion and accumulation) steadily decreased during this period from 3.1 on Day 0 to 1.35 pmol/embryo/h at the morula stage. However, at the blastocyst stage, turnover and balance increased to 6.32 and 2.42 pmol/embryo/h, respectively, i.e. net appearance occurred. Notable changes in amino acid profile during development included decreases in accumulation of asparagine, glutamate, and glycine in the medium and the depletion of glutamine over Days 0, 1, and 4, followed by reversal of these trends by Day 6. These data suggest that pig embryos can alter the accumulation and depletion rates of amino acids in a manner that is dependent on the specific amino acid and the stage of embryonic development. This work was supported by BBSRC.

scholarly journals The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities

1994 ◽  
Vol 72 (1) ◽  
pp. 59-68 ◽  
Author(s):  
A. Donkoh ◽  
P. J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Crude Protein ◽  
Apparent Digestibility ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Dietary Protein Level

The effect of dietary protein content (25, 60, 95, 130, 165 and 200 g crude protein (N × 6.25)/kg diet) on the apparent and true ileal digestibilities of N and amino acids in meat-and-bone meal given to the growing rat was investigated. Semi-synthetic diets in which meat-and-bone meal was the sole protein source were given to 180 g body-weight rats for 14 d. On the fourteenth day the rats were fed and then killed 4 h after the start of feeding and digesta were sampled from the terminal 200 mm ileum. Endogenous amino acid excretion was determined for eighteen rats given an enzymically hydrolysed casein (EHC)-based diet and with subsequent treatment of the digesta using ultrafiltration. The EHC-fed rats were killed 3 h after the start of feeding and digesta were collected from the terminal 200 mm ileum. True ileal digestibility values determined with reference to Cr as a marker were higher than the corresponding apparent estimates. Apparent digestibility values of N and amino acids increased significantly (P < 0.001) with increasing dietary protein level; however, dietary protein content had no significant (P > 0.05) effect on the true ileal digestibilities of N and amino acids. The mean apparent ileal digestibility of N in meat-and-bone meal ranged from 65.6 to 75.3%. The corresponding range for the true ileal digestibility of N was 76.9 to 78.2%. True ileal digestibility, unlike apparent digestibility, appears to be independent of dietary protein level and may allow feed ingredients to be compared accurately even if they are ingested in different quantities.

scholarly journals Chemical composition of wonderful kola (Bucchlozia coriacea) and breadfruit (Artocarpus altilis) seeds grown in south–south, Nigeria

2018 ◽  
Vol 53 (2) ◽  
pp. 125-132
Author(s):  
MO Aremu ◽  
DB Passali ◽  
H Ibrahim ◽  
RO Akinyeye
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Analytical Techniques ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Metabolizable Energy ◽  
Crude Fibre ◽  
Artocarpus Altilis ◽  
Limiting Amino Acid

The proximate and amino acid compositions of Artocarpus altilis and Bucchholzia coriacea were investigated using standard analytical techniques. The respective proximate composition (%) for the Artocarpus altilis and Bucchholzia coriacea samples were: Moisture (5.91 and 5.67); ash (4.39 and 5.13); crude fat (4.42 and 2.76); crude protein (12.27 and 14.78); crude fibre (7.10 and 6.27); carbohydrate (65.91 and 65.39). The calculated fatty acids and metabolizable energy for the Artocarpus altilis and Bucchholzia coriacea samples were 3.54 and 2.21 %; 1492.60 and 1465.01 kJ/100 g, respectively. The most abundant minerals in Artocarpus altilis and Bucchholzia were potassium (658.42 and 369.64 mg/100 g) and phosphorus (382.55 and 261.38 mg/100 g), respectively. The amino acid profile revealed that Artocarpus altilis and Bucchholzia coriacea samples contained nutritionally useful quantities of most of the essential amino acids. The total essential amino acids (TEAA) (with His) were 31.14 and 34.40 g/100 g crude protein for the Artocarpus altilis and Bucchholzia coriacea samples, respectively. The first limiting amino acid was Met + Cys (TSAA) for all the samples and calculated isoeletric points (ρl) were 3.52 and 4.05 for Artocarpus altilis and Bucchholzia coriacea, respectively.Bangladesh J. Sci. Ind. Res.53(2), 125-132, 2018

scholarly journals The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Maryane S F Oliveira ◽  
John K Htoo ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Crude Protein ◽  
Sole Source ◽  
Growing Pigs ◽  
Cereal Grains ◽  
Ileal Digestibility ◽  
Wheat Middlings ◽  
The Difference

Abstract An experiment was conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) in corn, wheat, and wheat middlings obtained using the direct procedure are not different from values obtained using the difference procedure. Sixteen ileal-cannulated barrows (initial BW: 69.5 ± 5.0 kg) were allotted to a replicated 8 × 4 Youden Square design with 8 diets and 4 periods. Each period consisted of 5 d of adaptation to the diet and 2 d of collection of ileal digesta. Four diets were based on soybean meal (SBM), corn, wheat, or wheat middlings as the only AA-containing ingredients. Three additional diets were based on a mixture of SBM and corn, SBM and wheat, or SBM and wheat middlings, and an N-free diet was also used. The apparent ileal digestibility (AID) and the SID of crude protein (CP) and AA for the 4 diets containing SBM, corn, wheat, or wheat middlings as the sole source of AA were calculated using the direct procedure. The AID and SID of CP and AA for the 3 mixed diets containing SBM and corn, wheat, or wheat middlings were also calculated and the contribution of digestible AA from SBM was subtracted from the AID or SID values for the diets. The AID or SID of AA in corn, wheat, and wheat middlings were subsequently calculated by difference. Results indicated that the AID values for a few AA were lower (P &lt; 0.05) if the direct procedure was used instead of the difference procedure, regardless of ingredient. The AID of Trp was greater in corn and wheat middlings, and the SID of Trp in corn and wheat middlings tended to be greater, if the direct procedure rather than the difference procedure was used, but that was not the case for wheat (interaction, P &lt; 0.05 and P &lt; 0.10, respectively). However, for all other indispensable AA, and for most of the dispensable AA, the SID of AA in corn, wheat, and wheat middlings was not different between the difference procedure and the direct procedure. Therefore, values for SID of AA in cereal grains and fiber-rich ingredients may be determined using either the direct or the difference procedure.

Endogenous amino acid flows at the terminal ileum of broilers, layers and adult roosters

2004 ◽  
Vol 79 (2) ◽  
pp. 265-271 ◽  
Author(s):  
V. Ravindran ◽  
W. H. Hendriks
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Terminal Ileum ◽  
Crude Protein ◽  
Amino Acid Profile ◽  
The Other ◽  
Endogenous Protein ◽  
Abundant Amino Acid ◽  
Endogenous Amino Acid

AbstractEndogenous flows of nitrogen and amino acids at the terminal ileum of broilers (6 weeks old), layers (70 weeks old) and adult roosters (70 weeks old) were determined using the peptide alimentation method. The ileal endogenous output of nitrogen and total amino acids in broilers, layers and roosters, expressed as mg/kg dry matter intake, were similar (F > 0-05). Endogenous flows were similar (F > 0-05) for nine of the 17 amino acids analysed, but the flows of serine, glutamic acid, proline, alanine, isoleucine, tyrosine, arginine and methionine differed (P < 0-05) among the classes of chickens. The amino acid profile of endogenous protein, expressed asg/100 g crude protein, did not differ (F > 0-05) between the three classes of chickens, except for serine, glutamic acid, proline and isoleucine. The concentrations of proline were higher (F < 0-05) in broilers, compared with the other two groups. The concentrations of glutamic acid in layers were lower (F < 0-05) than the other two groups. The concentrations of serine and isoleucine were higher (F < 0-05) in roosters than the other two groups. In all three groups, the most abundant amino acid in the ileal endogenous protein was glutamic acid, followed by aspartic acid, proline, serine, glycine and threonine. The present study provides estimates for endogenous amino acid flow at the terminal ileum in broilers, layers and adult roosters under normal physiological conditions.

scholarly journals The Proximate Composition, Amino Acid Profile and Chemical Indices in Five Freshwater Fishes from Tiga Dam Reservoir, Nigeria

2019 ◽  
Vol 77 (2) ◽  
pp. 87-92
Author(s):  
Audu Michael Elaigwu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Proximate Composition ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
High Energy ◽  
Dam Reservoir

Abstract The study assesses the proximate composition, amino acid profile and its content and chemical indices of the sun-dried Schilbe mystus, Bagrus bayad, Oreochromis niloticus, Clarias anguillaris and Petrocephalus bane bane from Tiga Dam Reservoir, Nigeria. The proximate composition varied significantly (p<0.05) in all five species of fish and these ranged as follows: Moisture (4.79 - 9.52 g/100 g), Crude Protein (42.20 - 57.71 g/100 g), Ash Content (0.90 - 12.51 g/100 g), Ether Extract (3.41 - 9.93 g/100 g), Crude Fibre (0.62 - 5.08 g/100 g), Nitrogen Free Extract (12.28 - 42.70 g/100 g) and Dry Matter (90.48 - 95.21 g/100 g). The amino acid also differed significantly (p<0.05). The nine essential amino acids found in the five species of fish were Lysine (4.21 - 6.34 g/100 g), Histidine (1.96 - 4.30 g/100 g), Arginine (5.80 - 8.21 g/100 g), Threonine (1.93 - 5.05 g/100 g), Valine (2.91 - 5.53 g/100 g), Methionine (1.74 - 3.80 g/100 g), Isoleucine (2.04 - 3.37 g/100 g), Leucine (3.64 - 7.18 g/100 g) and Phynylalanine (1.90 - 4.23 g/100 g). Whereas, the eight non-essential amino acids included: Serine (2.12 - 5.22 g/100 g), Glutamic acid (13.24 - 16.30 g/100 g), Proline (3.12 - 6.29 g/100 g), Glycine (4.20 - 9.08 g/100 g), Alanine (5.00 - 6.36 g/100 g), Cysteine (0.94 - 1.24 g/100 g), Tyrosine (2.33 - 3.33 g/100 g) and Aspartic acid (6.34 - 11.01 g/100 g). P. bane bane was first in terms of crude protein; S. mystus had the highest lipid and essential amino acid content. Also, C. anguillaris recorded the highest calorific value and best amino acid content. Thus, P. bane bane can serve as a source of animal protein to balance deficiencies in humans. Both S. mystus and C. anguillaris can be used as a nutrient base for high energy food and oil in the food industry.

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