scholarly journals In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Fluid ◽  
Ph Values

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>

scholarly journals In Vitro Fermentation Characteristics and Methane Mitigation Responded to Flavonoid Extract Levels from Alternanthera sissoo and Dietary Ratios

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acid Detergent Fiber ◽  
In Vitro Fermentation

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.

scholarly journals Glycerol inclusion levels in corn and sunflower silages

2014 ◽  
Vol 38 (5) ◽  
pp. 497-505 ◽  
Author(s):  
Adriana de Souza Martins ◽  
Juliane Ribeiro de Oliveira ◽  
Marili Lopes Lederer ◽  
José Luís Moletta ◽  
Shivelly Los Galetto ◽  
...  
Keyword(s):  
Seasonal Cycle ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
High Efficiency ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ph Values ◽  
Four Levels

Due to the seasonal cycle of forage, the use of silage to feed animals provides nutrients throughout the year. However, its quality can be improved with the inclusion of additives and other products. Glycerol is a rich source of energy and present a high efficiency of utilization by animals. The purpose of this work was to evaluate the effect of glycerol inclusion on the chemical and fermentation characteristics of corn and sunflower silages. Two silage sources (maize and sunflower) were used and four levels of glycerol inclusion (0, 15, 30 and 45%) based on dry matter were carried out. The experimental design was completely randomized in a 2 x 4 factorial arrangement with five replications. The pH values and chemical composition of corn and sunflower silages were determined. In both silages there was increment of dry matter, non-fiber carbohydrates and total digestible nutrients (TDN) added to a reduction of crude protein, neutral detergent fiber and acid detergent fiber due to the glycerol inclusion. The corn silage required 45% glycerol to achieve the TDN level of the sunflower silage. The glycerol addition contributed to the increase in the nutritional value, offsetting loss of quality in the ensiling process.

scholarly journals Voluntary Intake, Chemical Composition, and Nutrient Digestibility of Pangolagrass and Stargrass Hays

1969 ◽  
Vol 62 (4) ◽  
pp. 389-398
Author(s):  
R. Gutiérrez-Vargas ◽  
J. A. Arroyo-Aguilú ◽  
A. Ramírez-Ortiz
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Dry Matter Intake ◽  
Acid Detergent Fiber ◽  
Digitaria Decumbens ◽  
Cynodon Nlemfuensis ◽  
The Difference

Pangolagrass (Digitaria decumbens) and stargrass (Cynodon nlemfuensis) harvested at 30, 45, and 60 days and made in to hay, were fed to four castrated male ruminants (goats and steers) in individual digestion stalls. The grasses were fertilized at the rate of 4,480 kg/ha/yr with 15-5-10, harvested, sun-dried for 2 days and cut in 3- to 6-cm pieces. Each experimental period lasted 21 days, divided into a 14-day preliminary feed ing period and a 7-day collection period. Each hay was offered at a level of 90% intake, as determined in the preliminary period. Hays, offered and refused, and feces were weighed and sampled daily. Representative samples were analyzed for dry matter, crude protein, neutral-detergent fiber, acid-detergent fiber, cellulose, lignin, and silica. Hemicellulose was determined as the difference between neutral-detergent fiber and acid-detergent fiber. Apparent digestion coefficients for each fraction were calculated on all hays and all ruminants. Pangolagrass dry-matter intake was significantly (P < .01) greater than that of stargrass hay by both goats and steers. No significant differences in dry-matter intake were obtained between hay ages of 30, 45, and 60 days. Goats had a higher dry-matter intake (P < .01) than steers. Significant differences were obtained between hays in neutral-detergent fiber (P < .01), hemicellulose (P < .01), and cellulose (P < .05) contents and between hay ages in crude protein (P < .05), cellulose (P < .01), lignin (P < .05), and silica (P < .01) contents. For digestibilities, significant differences were obtained between hays in dry matter (P < .05), neutral-detergent fiber (P < 05), and cellulose (P < .01); between hay ages in lignin (P < .01) and silica (P < .01); and between ruminants in dry matter (P < .01), neutral-detergent fiber (P < .01), acid-detergent fiber (P < .01), hemicellulose (P < .01), and cellulose (P < .01). Goats may not necessarily substitute for steers in digestion trials; and lignin, but not silica, tends to be a valid marker for determining digestibility.

scholarly journals Effects of garlic supplementation on in vitro nutrient digestibility, rumen fermentation, and gas production

2021 ◽  
Vol 51 (2) ◽  
pp. 271-279
Author(s):  
M.R. Kekana ◽  
D. Luseba ◽  
M.C. Muyu
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Garlic Powder

Garlic contains secondary metabolites with antimicrobial properties that can alter nutrient digestibility and rumen fermentation, similar to other antimicrobial products. The objectives of the study were to evaluate the effects of garlic powder and garlic juice on in vitro nutrient digestibility, rumen fermentation, and gas production. The treatments consisted of control with no additives, garlic powder, and garlic juice at 0.5 ml and 1 ml. The digestibility of dry matter, crude protein and neutral detergent fibre were determined after 48 hours incubation. Rumen ammonia nitrogen and volatile fatty acids were determined at 12 hours and 24 hours incubation. The cumulative gas production was recorded periodically over 48 hours. The in vitro dry matter disappearance decreased with 1 ml of garlic juice compared with control. The crude protein degradability in garlic powder and garlic juice was lower than in control. Volatile fatty acids increased in all treatments. Individual volatile fatty acids were significantly different, especially propionate, whereas the acetate to propionate ratio was reduced by garlic juice, and ammonia nitrogen was reduced by garlic powder and 0.5 ml of garlic juice. The cumulative gas production increased significantly with both levels of garlic juice. The addition of garlic juice at 0.5 mL/100 ml could enhance the production of propionate, and reduce the acetate to propionate ratio, implying that the supply of hydrogen for methanogens was limited.

scholarly journals Biodiesel Co-products Modified the Rumen Parameters of Feedlot Lambs but did not Change Methane Production In Vitro*

2020 ◽  
Vol 48 ◽  
Author(s):  
Eliéder Prates Romanzini ◽  
Américo Garcia Da Silva Sobrinho ◽  
Roberta De Lima Valença ◽  
Thiago Henrique Borghi ◽  
Fernanda De Almeida Merlim ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Crude Glycerin ◽  
Apparent Digestibility ◽  
Feedlot Lambs

Background: Intensification of livestock is a strategy that increases productivity, but the diets used to increase animal production efficiency are composed mainly of corn and soybean, thereby increasing competition between animals and humans for the same food crops. This study evaluated nutrient intake, apparent digestibility of dry matter (DM) and nutrients, kinetics of gas production, and concentration of volatile fatty acids on diets with or without inclusion of biodiesel co-products formulated for feedlot lambs. So, the hypothesis is that replace of traditional ingredients by biodiesel co-products changes rumen parameters and methane emissions.Material, Methods & Results: The experiment was developed in São Paulo State University (Unesp), Jaboticabal/SP, Brazil, in Sheep Production Laboratory, which is owned to Animal Science Department. All trials developed in this study used a feedlot system, where animals were kept in individual pen. Forty Ile de France lambs male non-castrated were used in in vivo trial. To obtain rumen fluid, that was used in in vitro trial, four Santa Inês lambs with rumen cannula were used. The treatments evaluated were four diets: Control diet: roughage + concentrate; PM20: roughage + concentrate with peanut meal (PM) at 20% of DM; CG25: roughage + concentrate with crude glycerin (CG) at 25% of DM; and PMCG: roughage + concentrate with PM at 10% of DM and CG at 12.5% of DM. The roughage:concentrate ratio was 40:60 for all these diets. The parameters of the in vitro and in vivo experiments used were completely randomized with four treatments. When significant, the means between treatments were compared using Tukey test (P < 0.05). There was no effect of co-product inclusion on intake, except ether extract and neutral detergent fiber, which were higher for PM20 compared with CG25 diet. Apparent digestibility of dry matter (79.87%) and some nutrients (organic matter, crude protein, and neutral detergent fiber) was higher (P < 0.05) with CG25 diet. In vitro cumulative gas production was greater in CG25 and PMCG compared to the other diets, at early measurement points (2, 4, 6, and 10 h). The concentrations of methane, volatile fatty acids, and acetate:propionate ratio in vitro did not differ (P > 0.05) among diets.Discussion: Probably the high quality of the glycerin used (83.9% glycerol, 12.01% humidity, 3.79% salts, and 0.28% organic matter, no fat, as described by the manufacturer) may explain the low EE concentration observed in the diet using only crude glycerin and the observed lack of DMI effects in all diets. About apparent digestibility, the greater values measured for crude protein can be explained by superior synchronism during fermentation of the proteins and carbohydrates in the diet. The data showed that treatment CG25 obtained higher initial gas production, followed by treatment PMCG which contained 12.5% crude glycerin. Probably these results were caused by the greater apparent digestibility of DM in treatments that included crude glycerin. Our results of volatile fatty acid concentration are different from the decrease in molar proportion of acetic acid and increase of propionic acid described by several authors, with the inclusion of glycerin in the diet. However, the absence of effect by co-product inclusion on the molar proportion of acetic, propionic, and butyric acids measured in this trial reinforce the report from other authors that affirmed the same situation. Hence, the inclusion of PM at 20% of DM and CG at 25% of DM could successfully replace the traditional diets of feedlot lambs such as soybean and corn, respectively, without damages to intake and ruminal parameters in vitro.

scholarly journals Effect of Cashew Nutshell Oil Supplementation as Phenol Source for Protein Protection on In Vitro Nutrient Digestibility

2019 ◽  
Vol 43 (4) ◽  
Author(s):  
Rahma Fitriastuti ◽  
Lies Mira Yusiati ◽  
Budi Prasetyo Widyobroto ◽  
Zaenal Bachruddin ◽  
Chusnul Hanim
Keyword(s):  
Feed Efficiency ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Nutrient Digestibility ◽  
Tannin Content ◽  
Cashew Nutshell Liquid ◽  
Lowry Method

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.

scholarly journals Characteristics of Gut Microbiota in Sows and Their Relationship with Apparent Nutrient Digestibility

2019 ◽  
Vol 20 (4) ◽  
pp. 870 ◽  
Author(s):  
Qing Niu ◽  
Pinghua Li ◽  
Shuaishuai Hao ◽  
Sung Kim ◽  
Taoran Du ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Crude Protein ◽  
Animal Health ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Ether Extract ◽  
Microbial Structure ◽  
Acid Detergent Fiber ◽  
Digestive Process

The gut microbiota plays important roles in animal health and nutrient digestibility. The characteristics of gut microbiota population in grower pigs and their correlation with apparent nutrient digestibility were assessed in previous study. Here we studied characteristics of intestinal microbiota of sows and analyzed their relationships with apparent nutrient (ether extract (EE), crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF) and acid detergent fiber (ADF)) digestibility. Firmicutes and Bacteroidetes were the most dominant phyla, approximately 73% of the total sequences. Treponema, Oscillibacter and Lactobacillus were the most dominant generas, more than 49% of the total sequences. The microbiota of sows clustered separately from the microbiota of grower pigs at the age of D28 D60, D90 and D150. The abundance of Clostridium and Turicibacter was positively correlated with apparent EE digestibility. The abundance of Anaerofustis and Robinsoniella in sow fecal samples was positively correlated with apparent CF digestibility. The abundance of Collinsella and Sutterella was positively correlated with apparent NDF digestibility. The abundance of Clostridium, Collinsella, Robinsoniella and Turicibacter was positively correlated with apparent ADF digestibility. Sows have their unique gut microbial structure compared with grower pigs and some of them participate in the digestive process of different nutrients.

scholarly journals Study of wheat (Triticum aestivum L.) quality for feeding ruminants using in vitro and in vivo methods

2008 ◽  
Vol 53 (No. 6) ◽  
pp. 253-264 ◽  
Author(s):  
J. Pozdíšek ◽  
K. Vaculová
Keyword(s):  
Triticum Aestivum ◽  
Crude Protein ◽  
Nutritive Value ◽  
Nutrient Digestibility ◽  
Net Energy ◽  
Protein Nitrogen ◽  
Nitrogen Free Extract ◽  
Metabolisable Energy

: Nutrient digestibility and parameters of nutritive value for ruminants of two winter wheat (<I>Triticum aestivum</I> L.) cultivars were evaluated by means of an <I>in vivo</I> balance trial performed by the regression method on two groups of heifers with an increasing proportion of grain in DM (from 6 to 46%). Sulamit and Rapsodia, chosen on the basis of the <I>in vitro</I> test from a set of 25 cultivars (grown in 2002–2004) reached significant differences in DM fermentability <I>in vitro</I> (by 43.7–78.6 ml/g DM, <I>P</I> < 0.05). <I>In vivo</I> digestibility of crude protein, nitrogen-free extract, organic matter, parameters of N retention, energy concentrations (metabolisable energy, net energy for lactation and for fattening) and parameters in the PDI system (especially PDIE) increased along with the grain proportion in the diet. At the comparable proportion of grain in the ration the positive differences were significantly higher (<I>P</I> < 0.05) for Sulamit than Rapsodia.

scholarly journals In vitro fermentation of the rations containing Morinda citrifolia L. (Noni) using two types of inoculum

2019 ◽  
Vol 40 (2) ◽  
pp. 831 ◽  
Author(s):  
Luiz Juliano Valério Geron ◽  
Luiz Eduardo Cantão Veloso ◽  
Sílvia Cristina de Aguiar ◽  
Alexandre Lima de Souza ◽  
Ilda dos Santos Souza ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ph Value ◽  
Neutral Detergent Fiber ◽  
Morinda Citrifolia ◽  
In Vitro Digestibility ◽  
Ammoniacal Nitrogen ◽  
In Vitro Fermentation ◽  
Ph Values ◽  
Sheep Feces

The effect of inclusion of Morinda citrifolia L. (Noni) in standardized diets of sheep on in vitro digestibility and parameters fermentation was examined using two different inoculums (ruminal liquid and sheep feces). To determine the nutrients’ in vitro digestibility coefficient (IVDC), two sheep were used as inoculum donors. The experimental design was factorial (4 × 2), with four diets containing different proportions of Noni and two types of inoculum. The different dietary proportions of Noni (0%, 8%, 16%, and 24%) did not affect the IVDC of dry matter (DM), organic matter (OM) and crude protein (CP) for both inoculums. The IVDC of neutral detergent fiber (NDF) differed significantly between the inoculums. After in vitro incubation for 24 h, the pH value of the fermented material differed between the different diets and between the inoculums. Moreover, the concentration of ammoniacal nitrogen was affected by the proportion of Noni and differed between the inoculums after incubation for 24 h. The inclusion of up to 24% of Noni in standardized rations for ruminants did not affect the IVDC of DM, OM, CP, and NDF. The use of feces as inoculum for in vitro fermentation produced lower IVDC values than those by ruminal liquid. The diet containing 8% of Noni and fermented with ruminal liquid produced higher pH values after 24 h of incubation. Taken together, the different dietary Noni proportions and the different inoculums can alter the concentration of ammoniacal nitrogen of sheep diets after 24 h in vitro fermentation; however, they seem not to affect the IVDC of the nutrients.

scholarly journals In vitro degradation dynamics of neutral detergent fiber and silage quality of waste from production of heart of palm of peach palm

2021 ◽  
Vol 22 ◽  
Author(s):  
Samuel Galvão de FREITAS ◽  
Geraldo Fábio Viana BAYÃO ◽  
Augusto César de QUEIROZ ◽  
Robérson Machado PIMENTEL ◽  
Carlos Magno ROCHA Junior ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Leaf Sheath ◽  
Neutral Detergent Fiber ◽  
In Vitro Degradation ◽  
Fermentation Profile ◽  
Fermentation Parameters

ABSTRACT The objective of the present work was to evaluate the chemical composition, fermentation profile, and degradation parameters of the neutral detergent fiber of three silages made with the waste from production of heart of peach of palm, which consisted of leaf, leaf sheath, and their compound (55% leaf and 45% leaf sheath). The waste was packed in experimental silos and open after sixty days of fermentation; silage samples were collected for determination of chemical composition and degradation parameters of neutral detergent fiber at 0, 3, 6, 9, 12, 24, 36, 48, 72, and 96 h of incubation in vitro. A difference was detected in the dry matter, crude protein, neutral detergent fiber, acid detergent fiber, and lignin contents between the three types of silage. The leaf silage showed a higher fractional degradation rate and a higher potentially digestible fraction of neutral detergent fiber over the 96 h of incubation. As for the fermentative parameters, silage made with the leaf showed a higher pH (3,79) and lactic acid (1,18%), acetic acid (0,39%) and propionic acid (0,24%). The butyric acid was higher for compound silage (0,012%) and ammoniacal nitrogen was higher for sheath silage (0,94%). The leaf silage displayed better chemical characteristics, fermentation parameters and in vitro degradability properties, proving to be the silage with best nutritional value for feeding ruminants.

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