215 Effects of Reducing Crude Protein with Supplementation of Six Essential Amino Acids and Glutamate on Growth and Backfat of Finishing Pigs

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 115-115
Author(s):  
Marcos E Duarte ◽  
Sung Woo Kim
Keyword(s):  
Amino Acids ◽  
Glucose Metabolism ◽  
Growth Performance ◽  
Crude Protein ◽  
Essential Amino Acids ◽  
Blood Parameters ◽  
Fecal Microbiota ◽  
Nitrogen Utilization ◽  
Backfat Thickness ◽  
Finishing Pigs

Abstract This study evaluated the effects of low CP diets on growth performance of finishing pigs. Seventy-two pigs (54 kg BW) were allotted to 3 treatments (8 pens/treatment) based on RCBD with sex and initial BW as blocks. Pigs were fed for 56 d until 120 kg BW based on 3 phases (P1/2/3 for 21/17/18 d, respectively). Treatments were CON (18.0, 13.8, 12.7% CP for P1/2/3; supplementing Lys, Met, Thr, and Trp); LCP (13.5, 11.4, 10.4% CP; supplementing Lys, Thr, Trp, Met, Val, and Ile); and LCPG (14.1, 12.8, 11.1% CP; LCP + Glu to match % Glu with CON). All diets met NRC requirements and had 2.6 Mcal/kg NE. Growth performance and backfat thickness were measured by phase. At d 56, blood and fecal samples were collected to analyze blood parameters and fecal microbiota. Data were analyzed using SAS 9.4. Overall, the growth performance and backfat thickness did not differ among treatments. BUN of LCPG was lower (P < 0.05) than CON (4.03 to 6.05 mg/dL), whereas the insulin of LCPG was greater (P < 0.05) than CON (222 to 116 pmol/dL). Relative abundance of Streptococcaceae in CON (6.1%) was greater (P < 0.05) than LCP (2.0%) and LCPG (2.3%), whereas the abundance of Erysipelotrichaceae of CON (1.8) was greater (P < 0.05) than LCP (0.9%). Colectively, when Lys, Thr, Trp, Met, Val, and Ile were provided sufficiently and NE was maintained, CP can be reduced by 2.6% without affecting growth performance and backfat thickness compared to conventional diets with Lys, Met, Trp, and Trp. Feeding low CP diets reduced harmful bacteria, and Glu supplementation further enhanced nitrogen utilization and glucose metabolism. Application of low CP formulation with supplementation of 6 essential amino acids to finishing pigs can be successful, when NE is matched and further beneficial with Glu supplementation.

Amino acid requirements of the growing meat rabbit 2. Comparative growth performance on practical diets of equal lysine concentration but decreasing levels of other amino acids

1988 ◽  
Vol 47 (2) ◽  
pp. 303-310 ◽  
Author(s):  
W. H. Schultze ◽  
W. C. Smith ◽  
P. J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Growth Performance ◽  
Crude Protein ◽  
Regression Line ◽  
National Research Council ◽  
Essential Amino Acids ◽  
Whole Body ◽  
Asymptotic Model ◽  
Amino Acid Requirement

ABSTRACTOver a 40-day period nine rabbits were each given one of nine isocaloric diets with progressively reduced amounts of crude protein (159 to 97 g/kg) but a fixed level of lysine; the latter was slightly below the higher of the National Research Council (NRC, 1977) and Societe de Chime Organique et Biologique (AEC, 1978) recommendations. The proportion of each other essential amino acid in the diet of highest crude protein concentration was close to a balance derived from the higher of each independently determined amino acid requirement given by NRC (1977) and AEC (1978), while the proportions in the two diets of lowest crude protein level were below those in a theoretical amino acid balance based on rabbit whole-body tissue composition. When an intersecting linear regression line model was fitted to growth performance data over the period, performance was found to be similar on the first six diets (159 to 123 g crude protein per kg) but further reductions in dietary protein were accompanied by a decline in growth rate and concomitant increase in food conversion ratio. Fitting of an asymptotic model to the data showed that growth performance first declined markedly between diets four and five (130 to 120 g crude protein per kg). These findings suggest that if a balance of essential amino acids relative to lysine, based on current amino acid recommendations, is adhered to in diet formulation for the growing rabbit, several of these amino acids will be in excess of requirement

scholarly journals The effect of synthetic essential amino acids supplementation to low crude protein diets on growth performance and serum metabolites in broiler chickens

2019 ◽  
Vol 20 (3) ◽  
pp. 24-29 ◽  
Author(s):  
Ahmed Elshafey ◽  
Abeer Aziza ◽  
, Abdelhady Orma ◽  
Tarek Mohamed
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Growth Performance ◽  
Crude Protein ◽  
Body Weight Gain ◽  
Relative Weight ◽  
Essential Amino Acids ◽  
Controlled Study ◽  
Broiler Chicks ◽  
Final Body Weight

Objective: To assess the influence of reduction of crude protein (CP) by 5% and 10% with supplementation of crystalline synthetic essential amino acids (EAAs) on performance and carcass traits of broilers. Design: Randomized controlled study Animals: A total of 320 one-day-old broiler chicks, (Cobb 500) reared up to 35 days of age were randomly allocated into eight experimental groups with four replicates (10 each). Procedures: The treatments as follow; the first group of chicks were fed on control protein to meet the CP requirement and total AAs according to Cobb 500 recommendation; the second group were fed idle EAAs (Id EAAs) to meet the recommended CP and the digestible EAAs. The third, fourth, and fifth groups were fed on diet 5% reduction of CP with EAAs supplementation exceed the digestible EAAs requirement of Cobb by 110, 120, and 130% (LCP3+110EAAs, LCP4+120EAAs, LCP5+130EAAs). However, the sixth, seventh, eighth groups of chicks were fed on diet 10% reduction of CP with AAs supplementation exceed the digestible EAAs requirement of Cobb by 110, 120, and 130% (LCP6+110EAAs, LCP7+120EAAs, LCP8+130EAAs). Results: Broilers of group four, five, six, seven and eight showed a significant decrease final body weight (BW), body weight gain (BWG) and feed intake at 21 and 35 days of age. However, the same variables were significantly enhanced in broilers of group one, two, and three (p <0.05). Additionally, there was a significant decrease of relative weight of abdominal fat pad of broilers in groups three, four, five compared to other treatment groups. Serum uric acid and cholesterol were linearly decreased with reduction of CP and supplementation with synthetic essential amino acids. Conclusion and clinical relevance: The present results indicate that reduction of CP by 5% or 10% with crystalline EAAs supplementation by 110, 120 and 130% more than the digestible EAAs of broiler recommendation did not support the growth performance.

264 Effects of Low Dietary Crude Protein Diets Containing Coarse Wheat Bran as an Alternative to Zinc Oxide in Nursery Pig Diets

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 96-97
Author(s):  
Kelsey Hammers ◽  
Hilda I Calderon ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Robert D Goodband ◽  
...  
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Wheat Bran ◽  
Crude Protein ◽  
Essential Amino Acids ◽  
Feed Ratio ◽  
Nursery Pigs ◽  
Dietary Crude Protein ◽  
Nursery Pig ◽  
Reduced Crude Protein

Abstract An experiment was conducted to determine the effect of reduced crude protein (CP) in diets containing coarse wheat bran (CWB) without pharmacological levels of Zn (ZnO) on growth performance and fecal dry matter (DM) of nursery pigs. A total of 650 pigs (DNA 241′600; initially 6.6 kg), were used with 5 pigs/pen and 26 replicate pens/treatment. Pens were assigned to 1 of 5 treatments in a RCB design based on BW. Treatments were fed for 13-d, contained 4% CWB, and consisted of: 1) diet with pharmacological ZnO (2,000 mg/kg Zn) and 21% CP formulated to 1.35% standardized ileal digestible (SID) Lys; 2) a diet with 110 ppm added Zn and 21% CP (1.35% SID Lys); 3) a diet with 110 ppm added Zn formulated to 18% CP (1.20% SID Lys); 4) an 18% CP diet with 110 ppm added Zn formulated to 1.35% SID Lys by the addition of increased levels of feed grade amino acids; and 5) diet 4 with addition of non-essential amino acids (NEAA; glycine and glutamic acid). Data were analyzed using the lmer function in R. Overall, pigs fed 21% CP with ZnO had increased (P &lt; 0.05) ADG compared to those fed 18% CP (1.35% SID Lys) with high levels of feed grade amino acids or those fed the reduced SID Lys (1.2%) diet. Gain-to-feed ratio was increased (P &lt; 0.05) for pigs fed 21% CP diets and those fed the 18% CP diet with NEAA compared to pigs fed 1.2% SID Lys and pigs fed high levels of feed grade amino acids. Fecal DM was increased (P &lt; 0.05) for pigs fed reduced SID Lys diet. In conclusion, reducing CP (subsequently SID Lys) in diets without ZnO decreased growth performance but increased fecal DM.

scholarly journals Effect of different dietary protein levels and amino acids supplementation patterns on growth performance, carcass characteristics and nitrogen excretion in growing-finishing pigs

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yumei Zhao ◽  
Gang Tian ◽  
Daiwen Chen ◽  
Ping Zheng ◽  
Jie Yu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Dietary Protein ◽  
Crude Protein ◽  
Carcass Characteristics ◽  
Protein Diet ◽  
Nitrogen Excretion ◽  
Finishing Pigs ◽  
Protein Levels ◽  
Low Protein

Abstract Background This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance, carcass characteristics and nitrogen excretion in growing-finishing pigs. Forty-two barrows (25.00 ± 0.39 kg) were randomly assigned to 7 diets. Diet 1: the high crude protein diet with balanced for 10 essential amino acids (EAAs). Diet 2: the medium crude protein diet with 2% (approx) decreased protein level of Diet 1 and balanced 10 EAAs. Diet 3: the low crude protein diet with 4% decreased protein level of Diet 1 and balanced 10 EAAs. The protein levels of Diet 4, 5, 6 and 7 were the same as that of Diet 3. Diet 4 was only balanced for lysine (Lys), methionine (Met), threonine (Thr) and tryptophan (Trp); Diet 5 and 6 were further supplemented with extra isoleucine (Ile) or valine (Val), respectively; Diet 7 was further supplemented with extra Ile + Val. Results Over the 112 days trial, the reduction of dietary protein by 2% or 4% with balanced10 EAAs significantly decreased nitrogen excretion (P <  0.05), but had no effects on growth performance and carcass characteristics (P > 0.05). In low protein diet, Val supplementation significantly increased body weight gain at 25–50 kg phase (P <  0.05), while Ile supplementation at 75–100 kg phase and 100–125 kg phase significantly reduced the ratio of feed to gain (P <  0.05). No effect of different dietary protein levels and amino acids supplementation patterns in low protein diets on carcass characteristics was observed (P > 0.05). The total N excretion of pigs supplemented with only Lys, Met, Thr and Trp was numerically higher than that of pigs fed with extra Ile, or Val, or Ile + Val diets. Conclusion In low protein diet, Val is more required than Ile in the early growing phage (25–50 kg), while Ile becomes more required in the late growing and finishing phage (75–125 kg).

scholarly journals Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sonia Yun Liu ◽  
Shemil P. Macelline ◽  
Peter V. Chrystal ◽  
Peter H. Selle
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Chicken Meat ◽  
Meat Production ◽  
Accurate Identification ◽  
Reduced Crude Protein

AbstractThe prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry’s dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called “non-essential” amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.

scholarly journals 138 Effects of Increasing Soybean Meal in Corn-based Diets on Growth Performance of Late-finishing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 70-71
Author(s):  
Julia P Holen ◽  
Robert D Goodband ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Joel M DeRouchey
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Soybean Meal ◽  
Fixed Effects ◽  
Average Length ◽  
Average Daily Gain ◽  
Random Effect ◽  
Finishing Pigs ◽  
Distillers Grains With Solubles ◽  
Dietary Treatments

Abstract Two experiments were conducted to determine the effects of increasing levels of soybean meal (SBM) replacing feed grade amino acids in corn or corn-dried distillers grains with solubles (DDGS)-based diets on growth performance of late finishing pigs. In both experiments, there were 22 to 27 pigs per pen and 14 pens per treatment. Average length of the experiments was 35 (Exp. 1) and 29 days (Exp. 2). Diets were balanced to contain 0.70% SID Lys and 2,667 or 2,610 kcal NE/kg for Exp. 1 and 2, respectively. Minimum amino acid ratios relative to Lys were: Ile, 55; Met&Cys, 60; Thr, 65; Trp, 19.5, and Val, 70. Dietary crude protein ranged from 10.1 to 15.2 for Exp. 1 and 13.6 to 19.4 for Exp. 2. The statistical model considered fixed effects of treatment, linear and quadratic contrasts, and random effect of block. In Exp.1, 1,793 pigs (L337×1050, PIC; initially 104.9 ± 1.4 kg) were fed corn-based diets and pens of pigs were assigned to 1 of 5 dietary treatments with increasing SBM from 5 to 20%. Overall, average daily gain (ADG) and gain-to-feed (G:F) increased (linear; P &lt; 0.05) as SBM increased with the greatest improvement observed as SBM increased from 5 to 8.75%, with little improvement thereafter. In Exp. 2, 1,827 pigs (L337×1050, PIC; initially 97.9 ± 1.1 kg) were used in a similar study as Exp. 1, but all diets contained 25% DDGS and SBM levels increased from 0 to 16%. Overall, G:F and final bodyweight of pigs marginally improved (linear and quadratic, respectively; P &lt; 0.10) as SBM increased, with the greatest performance observed when diets contained 8% SBM. These results suggest that increasing SBM up to 8% at the expense of feed grade amino acids in corn or corn-DDGS-based diets improved ADG or G:F in late-finishing pigs.

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