scholarly journals 395 Effects of supplemental protein and yeast on fermentation of low quality forage in single-flow continuous culture fermenters

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 161-162
Author(s):  
Joshua M Zeltwanger ◽  
Allison M Meyer ◽  
Eric Bailey ◽  
Abe Scheaffer ◽  
Kevin Meng ◽  
...  
Keyword(s):  
Continuous Culture ◽  
Tall Fescue ◽  
Crude Protein ◽  
Distillers Grains ◽  
Dried Distillers Grains ◽  
N Concentration ◽  
Supplemental Protein ◽  
Single Flow ◽  
Over Time ◽  
Microbial N

Abstract Various anecdotes suggest the combination of supplemental protein and yeast improve utilization of low-quality forage. Two studies were conducted to evaluate effects of a commercially available supplement containing protein and yeast on fermentation in single-flow continuous culture fermenters. In Exp. 1, Bermuda grass with no supplement (NCON1), dried distillers grains (PCON1), and two commercially available supplements Diamond V XP (DV1) or SweetPro 16 (SP1) were compared. In Exp. 2, K31 tall fescue with no supplement (NCON2), supplemental dried distillers grains (PCON2), and two commercially available supplements Diamond V XP (DV2) or SweetPro 16 (SP2) were compared. Twenty-four single flow fermenters (1470mL) were randomly assigned to treatments and fed twice daily for 8 d, 5 d of adaptation and 3 d of collection. In both experiments, digestibility of DM, OM, NDF, and ADF were not influenced by supplementation regardless of source (P > 0.10). In Exp. 1, NH3-N concentration increased (P < 0.01) for DV1 over both NCON1 and PCON1 and was greatest for SP1 (P < 0.01). Greater amounts of NH3-N and microbial N were recovered from fermenter overflow when SP1 was supplemented (P < 0.01). Crude protein (CP) degradation was also greatest for SP1; however, when supplement was included, regardless of source, CP degradation was increased over NCON1. Acetate, propionate, butyrate, and total VFA levels were similar (P > 0.10) amongst NCON1, PCON1, and DV1 and greatest when SP1 was supplemented. In Exp. 2, fermenter NH3-N concentration was greatest for SP2 (P < 0.01) and similar amongst NCON2, PCON2, and DV2 (P > 0.01). No differences were detected (P > 0.10) between treatments with respects to CP degradation. Total VFA concentrations increased over time regardless of treatment during the second study.

CASE STUDY: Use of dried distillers grains for calves grazing tall fescue

2012 ◽  
Vol 28 (2) ◽  
pp. 227-233
Author(s):  
J.E. Williams ◽  
B.J. Corners ◽  
M.H. Ramos ◽  
M.R. Ellersieck
Keyword(s):  
Tall Fescue ◽  
Distillers Grains ◽  
Dried Distillers Grains

Effect of grain type and dried distillers grains plus solubles oil concentration on site of digestion in cattle fed finishing diets

2018 ◽  
Vol 98 (2) ◽  
pp. 368-375 ◽  
Author(s):  
Mary A. Rodenhuis ◽  
Faithe E. Keomanivong ◽  
Marc L. Bauer ◽  
Kendall C. Swanson
Keyword(s):  
Crude Protein ◽  
Distillers Grains ◽  
Starch Digestibility ◽  
Ether Extract ◽  
Finishing Diets ◽  
Dried Distillers Grains ◽  
Oil Concentration ◽  
Corn Distillers ◽  
Feeding Diets ◽  
Lipid Digestibility

The objective of this experiment was to determine the effects of grain type (rolled corn vs. rolled barley) and oil concentration of dried corn distillers grains plus solubles (DDGS; moderate = 7.9% vs. low = 4.5% ether extract) on site of digestion. Seven ruminally and duodenally cannulated Holstein steers (716 ± 8.9 kg) were used in a 4 × 7 Youden square with experimental periods of 24 d. True ruminal organic matter (OM) and crude protein (CP) digestibility (% of intake) as well as intestinal (% of entering duodenum) and total tract starch digestibility was greater (P ≤ 0.03) in steers fed diets containing barley than in steers fed diets containing corn. Ruminal disappearance and ruminal, intestinal, and total tract OM, CP, and starch digestibility were not influenced (P ≥ 0.11) by feeding diets containing DDGS with differing oil concentration. Total tract lipid digestibility (%) was greater (P < 0.001) in steers fed diets containing moderate-oil DDGS than low-oil DDGS. These data indicate that including a low-oil DDGS, as compared with a moderate-oil DDGS, in finishing diets containing rolled corn or rolled barley does not influence intake or site of digestion of nonlipid components.

Effect of dried distillers’ grains and solubles when replacing corn or soybean meal on rumen microbial growth in vitro as measured using DNA as a microbial marker

2014 ◽  
Vol 94 (2) ◽  
pp. 349-356 ◽  
Author(s):  
E. Castillo-Lopez ◽  
T. J. Klopfenstein ◽  
S. C. Fernando ◽  
P. J. Kononoff
Keyword(s):  
Soybean Meal ◽  
Crude Protein ◽  
Microbial Growth ◽  
Treatment Time ◽  
Distillers Grains ◽  
Yeast Cells ◽  
Fermentation Time ◽  
Dried Distillers Grains ◽  
Time Interaction

Castillo-Lopez, E., Klopfenstein, T. J., Fernando, S. C. and Kononoff, P. J. 2014. Effect of dried distillers’ grains and solubles when replacing corn or soybean meal on rumen microbial growth in vitro as measured using DNA as a microbial marker. Can. J. Anim. Sci. 94: 349–356. The objectives were to evaluate the use of rDNA markers to measure the effects of dried distillers’ grains with solubles (DDGS) and the potential treatment×time interaction on microbial crude protein (MCP) synthesis in vitro and secondly to measure the contribution of yeast based protein originating from DDGS. Treatments were: (1) CONT, control with no DDGS, but with alfalfa hay, corn silage, ground corn (GC) and soybean meal (SBM) included at 25% (DM basis); (2) LOWCORN, 20% DDGS (DM basis) replacing GC; (3) LOWSBM, 20% DDGS (DM basis) replacing SBM; and (4) LOWCORNSBM, 20% DDGS (DM basis) replacing 10% GC and 10% SBM. Treatments (0.5 g) were incubated in 50 mL of inoculum in duplicate. At 0, 4, 16, 32, 48 and 96 h of fermentation total DNA was extracted from each treatment and MCP was measured using rDNA markers. The sum of bacterial crude protein (BCP) and protozoal crude protein (PCP) was considered as MCP. Data were analyzed as a completely randomized design. The treatment×time interaction was tested and the SLICE option was included to evaluate the effect of treatment at each fermentation time point. There was a tendency to a treatment×time interaction (P=0.07) for MCP. Specifically, at 16 h, LOWCORNSBM yielded greater (P<0.05) MCP compared to either CONT or LOWCORN with estimates of 68.5, 33.8 and 23.3±8.9 mg g–1DM, for LOWCORNSBM, CONT and LOWCORN, respectively. At 48 h, however, LOWCORN yielded greater MCP (P<0.05) compared with LOWSBM with estimates of 72.2 and 32.5±8.9 mg g–1DM, for LOWCORN and LOWSBM, respectively. Yeast crude protein (YCP) was not affected (P=0.21) and averaged 0.04±0.02 mg g–1of substrate (DM basis). Overall, rDNA markers were effective for quantifying MCP, but further research on the methodology is needed. With DDGS inclusion, MCP was maintained; however, yeast cells were extensively degraded during fermentation.

scholarly journals Effects of bismuth subsalicylate and dietary sulfur level on fermentation by ruminal microbes in continuous culture

2017 ◽  
Vol 1 (4) ◽  
pp. 559-569 ◽  
Author(s):  
S. W. Fessenden ◽  
A. J. Carpenter ◽  
M. Ruiz-Moreno ◽  
T. C. Jenkins ◽  
M. D. Stern
Keyword(s):  
Continuous Culture ◽  
Corn Silage ◽  
Distillers Grains ◽  
Microbial Fermentation ◽  
Feedlot Performance ◽  
Bismuth Subsalicylate ◽  
Sulfur Level ◽  
In Vitro Rumen Fermentation ◽  
Microbial N

Abstract In ruminants, excess dietary sulfur can be associated with a reduction in DM intake, poor feedlot performance and sulfur-associated polioencephalomalacia. Bismuth subsalicylate (BSS) has been shown to decrease hydrogen sulfide in vitro. The objective of this experiment was to evaluate effects of BSS inclusion (0 or 0.5% of diet DM) and dietary sulfur (0.21 or 0.42% of diet DM) on microbial fermentation in continuous culture. Treatments were arranged in a 2 × 2 factorial design. Eight dual-flow continuous culture fermenters were used during 2 consecutive 10-d periods consisting of 7 d for stabilization followed by 3 d of sampling. A pelleted feedlot diet containing 39% dry rolled corn, 32% earlage, 21% wet distillers grains, 3.2% corn silage, 1.5% soybean meal, 0.6% urea and 2.7% mineral premix (DM basis) was provided as substrate for microbes at a rate of 75 g of DM × fermenter–1 × d–1. Effluents from sampling days were composited by fermenter within period, resulting in 4 replicates/treatment. Bismuth subsalicylate inclusion decreased (P &lt; 0.01) true OM digestion, while no effects were observed for NDF and ADF digestion. Total VFA concentrations, molar proportions of acetic, propionic, and branched-chained VFA decreased (P &lt; 0.01) with BSS addition. The ratio of acetic to propionic acid and the molar proportion of butyric acid increased (P &lt; 0.01) with BSS addition. In regard to nitrogen metabolism, BSS increased NH3–N concentration, NH3–N and dietary-N flows (P &lt; 0.01), and decreased non-NH3–N flow, microbial-N flow, CP degradation, and efficiency of microbial protein synthesis (P &lt; 0.01). Inclusion of BSS increased mean, minimum, and maximum fermentation pH (P &lt; 0.01). Amount of dietary sulfur and BSS inclusion influenced flows of amino acids and fatty acids from fermenters. Influences on fatty acid biohydrogenation and amino acid flows demonstrated an overall suppression of microbial fermentation. Results from this experiment indicate that BSS inclusion at 0.5% of diet DM has detrimental effects on in vitro rumen fermentation in continuous culture.

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