Effect of grain type and dried distillers grains plus solubles oil concentration on site of digestion in cattle fed finishing diets

2018 ◽  
Vol 98 (2) ◽  
pp. 368-375 ◽  
Author(s):  
Mary A. Rodenhuis ◽  
Faithe E. Keomanivong ◽  
Marc L. Bauer ◽  
Kendall C. Swanson

The objective of this experiment was to determine the effects of grain type (rolled corn vs. rolled barley) and oil concentration of dried corn distillers grains plus solubles (DDGS; moderate = 7.9% vs. low = 4.5% ether extract) on site of digestion. Seven ruminally and duodenally cannulated Holstein steers (716 ± 8.9 kg) were used in a 4 × 7 Youden square with experimental periods of 24 d. True ruminal organic matter (OM) and crude protein (CP) digestibility (% of intake) as well as intestinal (% of entering duodenum) and total tract starch digestibility was greater (P ≤ 0.03) in steers fed diets containing barley than in steers fed diets containing corn. Ruminal disappearance and ruminal, intestinal, and total tract OM, CP, and starch digestibility were not influenced (P ≥ 0.11) by feeding diets containing DDGS with differing oil concentration. Total tract lipid digestibility (%) was greater (P < 0.001) in steers fed diets containing moderate-oil DDGS than low-oil DDGS. These data indicate that including a low-oil DDGS, as compared with a moderate-oil DDGS, in finishing diets containing rolled corn or rolled barley does not influence intake or site of digestion of nonlipid components.

2014 ◽  
Vol 94 (2) ◽  
pp. 349-356 ◽  
Author(s):  
E. Castillo-Lopez ◽  
T. J. Klopfenstein ◽  
S. C. Fernando ◽  
P. J. Kononoff

Castillo-Lopez, E., Klopfenstein, T. J., Fernando, S. C. and Kononoff, P. J. 2014. Effect of dried distillers’ grains and solubles when replacing corn or soybean meal on rumen microbial growth in vitro as measured using DNA as a microbial marker. Can. J. Anim. Sci. 94: 349–356. The objectives were to evaluate the use of rDNA markers to measure the effects of dried distillers’ grains with solubles (DDGS) and the potential treatment×time interaction on microbial crude protein (MCP) synthesis in vitro and secondly to measure the contribution of yeast based protein originating from DDGS. Treatments were: (1) CONT, control with no DDGS, but with alfalfa hay, corn silage, ground corn (GC) and soybean meal (SBM) included at 25% (DM basis); (2) LOWCORN, 20% DDGS (DM basis) replacing GC; (3) LOWSBM, 20% DDGS (DM basis) replacing SBM; and (4) LOWCORNSBM, 20% DDGS (DM basis) replacing 10% GC and 10% SBM. Treatments (0.5 g) were incubated in 50 mL of inoculum in duplicate. At 0, 4, 16, 32, 48 and 96 h of fermentation total DNA was extracted from each treatment and MCP was measured using rDNA markers. The sum of bacterial crude protein (BCP) and protozoal crude protein (PCP) was considered as MCP. Data were analyzed as a completely randomized design. The treatment×time interaction was tested and the SLICE option was included to evaluate the effect of treatment at each fermentation time point. There was a tendency to a treatment×time interaction (P=0.07) for MCP. Specifically, at 16 h, LOWCORNSBM yielded greater (P<0.05) MCP compared to either CONT or LOWCORN with estimates of 68.5, 33.8 and 23.3±8.9 mg g–1DM, for LOWCORNSBM, CONT and LOWCORN, respectively. At 48 h, however, LOWCORN yielded greater MCP (P<0.05) compared with LOWSBM with estimates of 72.2 and 32.5±8.9 mg g–1DM, for LOWCORN and LOWSBM, respectively. Yeast crude protein (YCP) was not affected (P=0.21) and averaged 0.04±0.02 mg g–1of substrate (DM basis). Overall, rDNA markers were effective for quantifying MCP, but further research on the methodology is needed. With DDGS inclusion, MCP was maintained; however, yeast cells were extensively degraded during fermentation.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 139-139
Author(s):  
Aksel Wiseman ◽  
Brad Boyd ◽  
Levi McPhillips ◽  
Scott Tilton ◽  
Andrea K Watson ◽  
...  

Abstract A 112 d finishing study was conducted to evaluate the effects of feeding dried distillers grains plus solubles (DDGS) from the MSC-Fiber stream of the FluidQuip post-fermentation fiber separation process compared to conventional DDGS. Yearling crossbred steers (n = 240, initial BW = 463 ± 35kg) were blocked by initial BW, stratified by BW, and assigned randomly to pen (n = 30; 8 steers/pen). Treatments were arranged as a 2×2 + 1 factorial with DDGS type (MSC DDGS [MSC]; Conventional DDGS [CONV]) and inclusion level (20 or 40% diet DM) as the factors. All treatments were compared to a corn-based control (CON). DDGS replaced a 60:40 blend of high-moisture corn and dry-rolled corn. All diets contained 7.5% alfalfa hay and supplement. Data were analyzed as a randomized block design with pen as experimental unit and block as fixed effect. Increasing concentrations of CONV or MSC resulted in a linear increase in DMI (P &lt; 0.01). Steers fed 40% MSC had the greatest DMI (P &lt; 0.01) while CON steers had the least (P &lt; 0.02). Daily gains for MSC steers linearly increased (P = 0.03), while CONV steers showed a tendency for a quadratic response to ADG (P=0.08). Feed efficiency of CONV steers increased quadratically (P = 0.04) and tended to decrease linearly (P = 0.09) for steers fed MSC. Efficiency was greatest for 20% CONV but not different (P &gt; 0.16) than CON, or 20% MSC. Steers fed 40% MSC or 40% CONV were not different in feed efficiency. Inclusion of MSC DDGS resulted in linear increases in DMI and ADG over CON, but decreased feed efficiency by 1.0 and 5.3% resulting in MSC DDGS having a relative feeding value of 95.0 and 86.7% of corn at 20 and 40% DM, respectively.


2014 ◽  
Vol 77 (11) ◽  
pp. 1968-1975 ◽  
Author(s):  
ROBIN C. ANDERSON ◽  
ROGER B. HARVEY ◽  
TRYON A. WICKERSHAM ◽  
JIM C. MacDONALD ◽  
CHRISTIAN H. PONCE ◽  
...  

Campylobacter bacteria are foodborne pathogens that can colonize the gut of food animals. Limited in their ability to ferment sugars, Campylobacter can derive energy for growth via amino acid catabolism. The objectives of the present studies were to test whether supplemental distillers grains containing high amounts of rumen-undegradable intake protein or supplemental lasalocid may, by promoting amino acid flow to the lower bovine gut, increase intestinal carriage of Campylobacter. In study one, 10 steers (5 per treatment) were adapted to diets formulated to achieve 0 or 30% dried distillers grains. After an initial 14-day adaptation to the basal diet, control and treated steers were fed their respective diets for 23 days, after which time they were fed supplemental lasalocid for an additional 8 days, followed by a 5-day withdrawal. In study two, 24 steers preacclimated to a basal diet were adapted via 3-day periodic increases to dietary treatments formulated to achieve 0, 30, or 60% wet corn distillers grains with solubles. Analysis of Campylobacter bacteria cultured from duodenal and fecal samples in study one and from fecal samples in study two revealed no effect of dried distillers grains or wet corn distillers grains with solubles on the prevalence or concentrations of duodenal or fecal Campylobacter. The results from study one indicated that colonized steers, regardless of treatment, harbored higher Campylobacter concentrations when transitioned to the basal diet than when coming off pasture. Campylobacter carriage was unaffected by lasalocid. These results provide no evidence that feeding distillers grains high in rumen-undegradable intake protein or supplemental lasalocid contributes to increased intestinal carriage of Campylobacter in fed cattle.


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