Placenta Expressing the Greatest Quantity of Bisphenol A Receptor ERR  among the Human Reproductive Tissues: Predominant Expression of Type-1 ERR  Isoform

The recent discovery of microRNAs (miRNAs) in human reproductive tissues and cells indicates a possible functional role in reproductive function. However, the studies published to date in female reproductive tissues/cells and embryos are inconclusive and sometimes controversial. In order to update the knowledge of this field, the present study aimed to discuss, through a systematic review, the role of miRNAs in female human reproduction and early embryogenesis. We conducted a systematic review of the published literature in MEDLINE and EMBASE databases through June 2018 (plus a complementary search until July 2019), in accordance with the PRISMA guidelines. We have included descriptive and observational studies, in which fertile/infertile women were well-defined. The primary outcome was the miRNA expression in ovaries, oocytes, extracellular vesicles, and embryos. We identified 25,204 articles, of which 28 were selected for qualitative analysis: 18 in ovaries and extracellular vesicles, three in oocytes, and seven in embryos. The present systematic review of descriptive and observational studies demonstrates that aberrant miRNA expression in female reproductive tissues/cells and embryos is related with infertility and embryogenesis errors. The expression of specific miRNAs, particularly in extracellular vesicles, may be used in the future as biomarkers of infertility and prognostic tools of embryo development.

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Macrophages in Human Reproductive Tissues Contain Luteinizing Hormone/Chorionic Gonadotropin Receptors

American Journal Of Reproductive Immunology ◽

10.1034/j.1600-0897.2003.00013.x ◽

2003 ◽

Vol 49 (2) ◽

pp. 93-100 ◽

Cited By ~ 34

Author(s):

Y. M. Zhang ◽

Ch. V. Rao ◽

Z. M. Lei

Keyword(s):

Luteinizing Hormone ◽

Chorionic Gonadotropin ◽

Gonadotropin Receptors ◽

Reproductive Tissues ◽

Human Reproductive

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118. THE PRORENIN RECEPTOR/PLZF PATHWAY IN HUMAN AMNION

Reproduction Fertility and Development ◽

10.1071/srb10abs118 ◽

2010 ◽

Vol 22 (9) ◽

pp. 36

Author(s):

K. G. Pringle ◽

A. L. Conquest ◽

C. M. Mitchell ◽

T. Zakar ◽

E. R. Lumbers

Keyword(s):

New Zealand ◽

Amniotic Fluid ◽

Mrna Levels ◽

The Novel ◽

Quantitative Real Time Pcr ◽

Human Amnion ◽

Reproductive Tissues ◽

Prorenin Receptor ◽

Human Reproductive ◽

Neonatal Physiology

Prorenin, despite being inactive, is the major form of renin found in amniotic fluid and reproductive tissues. Prorenin becomes active if it binds to the novel prorenin receptor (ATP6AP2). The prorenin-ATP6AP2 complex has been found to stimulate translocation of Promyelocytic Zinc Finger (PLZF) protein to the nucleus where it increases expression of the p85α subunit of PI3 kinase (PI3K-p85α) and represses the expression of ATP6AP21. Progesterone and glucocorticoids have also been shown to stimulate PLZF2, 3. We aimed to find out if PLZF and the prorenin-ATP6AP2 pathway interact in human reproductive tissues. Human amnion was cultured for 24 h in media containing vehicle, dexamethasone, amniotic fluid or recombinant human (rh) prorenin. Total RNA was extracted using TRIZol® and converted to cDNA for quantitative real-time PCR using SuperScript III and random hexamers. mRNA abundances for PLZF, PI3K-p85α and ATP6AP2 were calculated relative to Alien RNA using the ΔΔCT method. Our preliminary data show that exposure of amnion explants to dexamethasone upregulates PLZF and PI3K-p85α mRNA but has no effect on ATP6AP2. Culture of amnion explants with amniotic fluid also increases PLZF but does not change PI3K or ATP6AP2. In contrast, culture of amnion explants with (rh) prorenin increases PI3K mRNA but not PLZF or ATP6AP2. As expected, dexamethasone affects PLZF expression, however in amnion there is no interaction with the ATP6AP2 pathway. In addition, we believe we have identified a novel prorenin/ATP6AP2 signalling pathway which acts on PI3K-p85α independent of PLZF. In contrast to these data, amniotic fluid increases PLZF but not PI3K-p85α mRNA levels suggesting that amniotic fluid contains other factors that oppose prorenin and glucocorticoid effects on PI3K-p85α. (1) Schefe, J.H., et al. Circ Res, 2006. 99(12): 1355–1366.(2) Fahnenstich, J., et al. Mol. Hum. Reprod., 2003. 9(10): 611–623.(3) Conquest, A. et al. Fetal and Neonatal Physiology Workshop, 2010. Wellington, New Zealand.

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Inhibition of Type 1 Iodothyronine Deiodinase by Bisphenol A

Hormone and Metabolic Research ◽

10.1055/a-0919-3879 ◽

2019 ◽

Vol 51 (10) ◽

pp. 671-677 ◽

Cited By ~ 7

Author(s):

Maurício Martins da Silva ◽

Carlos Frederico Lima Gonçalves ◽

Leandro Miranda-Alves ◽

Rodrigo Soares Fortunato ◽

Denise P. Carvalho ◽

...

Keyword(s):

Adipose Tissue ◽

Thyroid Hormone ◽

Bisphenol A ◽

Brown Adipose Tissue ◽

Tissue Type ◽

Deiodinase Activity ◽

Brown Adipose

AbstractPlastics are ubiquitously present in our daily life and some components of plastics are endocrine-disrupting chemicals, such as bisphenol A and phthalates. Herein, we aimed to evaluate the effect of plastic endocrine disruptors on type 1 and type 2 deiodinase activities, enzymes responsible for the conversion of the pro-hormone T4 into the biologically active thyroid hormone T3, both in vitro and in vivo. Initially, we incubated rat liver type 1 deiodinase and brown adipose tissue type 2 deiodinase samples with 0.5 mM of the plasticizers, and the deiodinase activity was measured. Among them, only BPA was capable to inhibit both type 1 and type 2 deiodinases. Then, adult male Wistar rats were treated orally with bisphenol A (40 mg/kg b.w.) for 15 days and hepatic type 1 deiodinase and brown adipose tissue type 2 deiodinase activities and serum thyroid hormone concentrations were measured. In vivo bisphenol A treatment significantly reduced hepatic type 1 deiodinase activity but did not affect brown adipose tissue type 2 deiodinase activity. Serum T4 levels were higher in bisphenol A group, while T3 remained unchanged. T3/T4 ratio was decreased in rats treated with bisphenol A, reinforcing the idea that peripheral metabolism of thyroid hormone was affected by bisphenol A exposure. Therefore, our results suggest that bisphenol A can affect the metabolism of thyroid hormone thus disrupting thyroid signaling.

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Recent Advances in Electrochemical Sensors and Biosensors for Detecting Bisphenol A

Sensors ◽

10.3390/s20123364 ◽

2020 ◽

Vol 20 (12) ◽

pp. 3364 ◽

Cited By ~ 4

Author(s):

Somayeh Tajik ◽

Hadi Beitollahi ◽

Fariba Garkani Nejad ◽

Kaiqiang Zhang ◽

Quyet Van Le ◽

...

Keyword(s):

Bisphenol A ◽

Electrochemical Sensors ◽

Environmental Pollutants ◽

Modified Electrodes ◽

Recent Decade ◽

Raw Material ◽

Reliable Determination ◽

Chemically Modified ◽

Obesity And Diabetes ◽

Human Reproductive

In recent years, several studies have focused on environmental pollutants. Bisphenol A (BPA) is one prominent industrial raw material, and its extensive utilization and release into the environment constitute an environmental hazard. BPA is considered as to be an endocrine disruptor which mimics hormones, and has a direct relationship to the development and growth of animal and human reproductive systems. Moreover, intensive exposure to the compound is related to prostate and breast cancer, infertility, obesity, and diabetes. Hence, accurate and reliable determination techniques are crucial for preventing human exposure to BPA. Experts in the field have published general electrochemical procedures for detecting BPA. The present timely review critically evaluates diverse chemically modified electrodes using various substances that have been reported in numerous studies in the recent decade for use in electrochemical sensors and biosensors to detect BPA. Additionally, the essential contributions of these substances for the design of electrochemical sensors are presented. It has been predicted that chemically modified electrode-based sensing systems will be possible options for the monitoring of detrimental pollutants.

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