miR-146a and miR-196a-2 genes polymorphisms and its circulating levels in lung cancer patients

AbstractRecently, MicroRNAs polymorphisms and their serum expression have been linked to increase risk of various cancers. The aim of this study was to elucidate the association between single nucleotide polymorphisms of miR-146a and miR-196a-2 and their serum expression and lung cancer risk. One hundred and twenty lung cancer patients and 120 health controls were included in this study. Genotyping and expression for miR-146a and miR-196a-2 were performed using polymerase chain reaction (PCR)-restriction fragment length polymorphism and quantitative real-time PCR. Individuals carrying miR-146a CG and CC genotypes had significantly increased risk for lung cancer than those carrying miR-146a GG genotype. MiR-146a expression significantly decreased in miR-146a CG and CC genotypes carriers as compared with GG genotype carriers. MiR-196a-2 CT and TT genotypes were significantly associated with increased lung cancer while the highest expression of MiR-196a-2 was detected in miR-196a-2 CC genotype carriers. Serum miR-146a was significantly decreased in lung cancer patients while serum miR-196a-2 expression was significantly increased in lung cancer patients. In conclusion, miR-146a and miR-196a-2 genes polymorphisms and their circulating levels were associated with lung cancer risk in Egyptians and may be helpful in early detection of lung cancer.

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Influence of CMTM8 polymorphisms on Lung Cancer Susceptibility in the Chinese Han Population

10.21203/rs.3.rs-34577/v1 ◽

2020 ◽

Author(s):

Jiamin Wu ◽

Yao Sun ◽

Zichao Xiong ◽

Fanglin Niu ◽

Yuanwei Liu ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Risk ◽

Cancer Patients ◽

Lung Cancer Risk ◽

Lung Squamous Cell Carcinoma ◽

Chinese Han Population ◽

Chinese Han ◽

Han Population ◽

Lung Cancer Patients ◽

Increased Risk

Abstract Objective: Lung cancer is the leading cause of cancer-related mortality worldwide and CMTM8 is a potential tumor suppressor gene, which is down-regulated in lung cancer. The objective of this research was to assess the association of CMTM8 genetic polymorphisms with lung cancer risk in Chinese Han population.Methods: To evaluate the correlation between CMTM8 polymorphisms and lung cancer risk, Agena MassArray platform was used for genotype determination among 509 lung cancer patients and 506 controls. Multiple genetic models, stratification analysis and haploview analysis was used by calculating odds ratio (OR) and 95% confidence intervals (CIs).Results: Significant associations were detected between CMTM8 rs6771238 and an increased lung cancer risk in codominant (adjusted OR = 1.57, 95%CI: 1.01-2.42, p = 0.044) and dominant (adjusted OR = 1.54, 95%CI: 1.01-2.36, p = 0.047)models. After gender stratification analysis,weobserved that rs6771238 was related to an increased risk of lung squamous cell carcinoma, while rs6771238 was associated with anincreased risk of lung adenocarcinoma. Rs9835916 and rs1077868 were correlated with lung cancer staging.Rs9835916 was linked to increased risk of lymph node metastasis in lung cancer patients. Conclusions: Our study firstly reported that the CMTM8 polymorphisms were a risk factors for lung cancer, which suggested the potential roles of CMTM8 in the development of lung cancerin Chinese Han population.

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Diabetes And Lung Cancer; A Sweet And Sour Relationship

Current Respiratory Medicine Reviews ◽

10.2174/1573398x17666210917100152 ◽

2021 ◽

Vol 17 ◽

Author(s):

Andreas Paraskevas ◽

Elena Bellou ◽

Elli Keramida ◽

Irma Bracka ◽

Christina Gkriniouk ◽

...

Keyword(s):

Diabetes Mellitus ◽

Lung Cancer ◽

Cancer Risk ◽

Cancer Patients ◽

Human Insulin ◽

Lung Cancer Risk ◽

Survival Rates ◽

Diabetic Patients ◽

Lung Cancer Patients ◽

Increased Risk

Introduction: Diabetes mellitus and lung cancer are two highly prevalent diseases on a global scale. The association between these two diseases has been the subject of study of many cohorts in the past, some of which presenting inconclusive results. Aim: To present existing evidence regarding the association of diabetes with lung cancer risk and survival as well as the impact of metformin and insulin on lung cancer. Methods: A narrative review of all the relevant published literature known to the authors was conducted. The references were identified by searching the Pubmed online database using the keywords such as diabetes and lung cancer risk, diabetes and lung cancer survival rates, metformin and lung cancer, insulin and lung cancer. Results : Although previous epidemiologic studies have failed to report any significant causal effect of diabetes on lung cancer risk, recent evidence from 2 large scale meta-analyses of observational studies and other studies showed a statistically significant correlation between nonsmoker diabetics and lung cancer risk (RR, 1.11; 95% CI, 1.02-1.20; I2 = 46.1% and RR, 1.14; 95% CI, 1.09-1.20; I2 = 0%). Recent evidence has shown that metformin is related to a lower risk of lung cancer incidence among never-smokers diabetic patients (HR, 0.57;95% CI, 0.33-0.99) and improved overall survival rates (HR=0.77, 95%CI=0.66-0.9, p=0.001) regardless of the histological type of lung cancer. On the other hand, a recently published large population-based retrospective cohort study has concluded that human insulin is associated with an increased risk of lung cancer (HR 1.545, 95% CI: 1.478-1.614). Interestingly, metformin has been reported to have an anti-resistant effect on lung cancer patients who are on Epidermal Growth Factor Receptor- Tyrosine Kinase Inhibitors, offering some protection against resistance to therapy. Conclusion: Published evidence shows that diabetes mellitus is associated with an increased risk of lung cancer and worse survival rates. Metformin plays a protective role in lung cancer pathogenesis and is also associated with prolonged progression-free survival in lung cancer patients with diabetes. There is a significant association between human insulin use and lung cancer risk. More randomized controlled studies are required to establish the positive association of diabetes and anti-diabetic drugs with lung cancer, taking into further consideration gender and lung cancer pathology as well as possible confounders such as smoking habits that can influence the results of these studies.

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Influence of CMTM8 polymorphisms on Lung cancer susceptibility in the Chinese Han population

10.21203/rs.2.12045/v2 ◽

2019 ◽

Author(s):

Jiamin Wu ◽

Yao Sun ◽

Zichao Xiong ◽

Fanglin Niu ◽

Yuanwei Liu ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Risk ◽

Cancer Patients ◽

Lung Cancer Risk ◽

Cancer Staging ◽

Chinese Han Population ◽

Chinese Han ◽

Han Population ◽

Lung Cancer Patients ◽

Increased Risk

Abstract Abstract Background: Lung cancer is the leading cause of cancer-related mortality worldwide and CMTM8 is a potential tumor suppressor gene, which is down-regulated in lung cancer. The objective of this research was to assess the association of CMTM8 genetic polymorphisms with lung cancer risk in Chinese Han population. Methods: To evaluate the correlation between CMTM8 polymorphisms and lung cancer risk, Agena MassArray platform was used for genotype determination among 509 lung cancer patients and 506 controls. Multiple genetic models, stratification analysis and haploview analysis were used by calculating odds ratio (OR) and 95% confidence intervals (CIs). Results: Significant associations were detected between CMTM8 rs6771238 and an increased lung cancer risk (p < 0.05). In stratified analysis, rs6771238 was related to an increased risk of lung squamous cell carcinoma (p < 0.05), rs6771238 was associated with an increased risk of lung adenocarcinoma (p < 0.05), rs9835916 and rs1077868 were correlated with lung cancer staging (p < 0.05), and rs9835916 was correlated with an increased risk of lymph node metastasis in lung cancer patients (p < 0.05). Additionally, Haplotype analysis illuminated that haplotypes GG and AG were closely correlated with lung cancer staging, and haplotype AG was correlated with an increased lung cancer risk among individuals older than 50 years (p < 0.05). Conclusions: Our study firstiy reported that the CMTM8 polymorphisms were risk factors for lung cancer in Chinese Han population. These findings also suggested the potential roles of CMTM8 in the development of lung cancer.

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Influence of CMTM8 polymorphisms on Lung cancer susceptibility in the Chinese Han population

10.21203/rs.2.12045/v1 ◽

2019 ◽

Author(s):

Jiamin Wu ◽

Yao Sun ◽

Zichao Xiong ◽

Fanglin Niu ◽

Yuanwei Liu ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Risk ◽

Cancer Patients ◽

Lung Cancer Risk ◽

Cancer Staging ◽

Chinese Han Population ◽

Chinese Han ◽

Han Population ◽

Lung Cancer Patients ◽

Increased Risk

Abstract Background: Lung cancer is the leading cause of cancer-related mortality worldwide and CMTM8 is a potential tumor suppressor gene, which is down-regulated in lung cancer. The objective of this research was to assess the association of CMTM8 genetic polymorphisms with lung cancer risk in Chinese Han population. Methods: To evaluate the correlation between CMTM8 polymorphisms and lung cancer risk, Agena MassArray platform was used for genotype determination among 509 lung cancer patients and 506 controls. Multiple genetic models, stratification analysis and haploview analysis was used by calculating odds ratio (OR) and 95% confidence intervals (CIs). Results: Significant associations were detected between CMTM8 rs6771238 and an increased lung cancer risk (p < 0.05). In stratified analysis, rs6771238 was related to increased risk of lung squamous cell carcinoma (p < 0.05), rs6771238 was associated with increased risk of lung adenocarcinoma (p < 0.05), rs9835916 and rs1077868 were correlated with lung cancer staging (p < 0.05), and rs9835916 was correlated with increased risk of lymph node metastasis in lung cancer patients (p < 0.05). Additionally, Haplotype analysis illuminated that haplotypes GG and AG were closely correlated with lung cancer staging, and haplotype AG was correlated with increased lung cancer risk among individuals older than 50 years (p < 0.05). Conclusions: Our study first reported that the CMTM8 polymorphisms were risk factors for lung cancer in Chinese Han population. These findings also suggested the potential roles of CMTM8 in the development of lung cancer.

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Analysis of Erythrocyte Glycophorin-A Variants by Flow Cytometry in Lung Disease Patients Detects the Effect of Tobacco Smoke

Analytical Cellular Pathology ◽

10.1155/2000/512786 ◽

2000 ◽

Vol 21 (1) ◽

pp. 35-40 ◽

Cited By ~ 2

Author(s):

Monica Neri ◽

Elio Geido ◽

Rosangela Filiberti ◽

Roberto Orecchia ◽

Angela Di Vinci ◽

...

Keyword(s):

Lung Cancer ◽

Flow Cytometry ◽

Cancer Risk ◽

Tobacco Smoke ◽

Lung Cancer Risk ◽

Small Population ◽

Cancer Risk Assessment ◽

Lung Cancer Patients ◽

Increased Risk

The glycophoryn A (GPA) assay evaluates somaticin vivomutations. It is considered a cumulative biodosimeter for genotoxic exposures and is under evaluation in cancer risk assessment.GPA, a polymorphic membrane protein of the erythrocytes, determines the MN blood groups. The N0 and NN variant frequencies (VF) may be detected in MN subjects (about 50% of the population) by flow cytometry using two differently labelled antibodies.We explored if GPA N0 and NN VF might be relevant to the assessment of individual lung cancer risk and susceptibility, in a small population with a high prevalence of heavy tobacco smokers: 8 lung cancer patients and 16 subjects with non‐malignant lung diseases associated with increased risk of lung cancer.There was a wide interindividual variability and complete overlap between non‐neoplastic and neoplastic patients. A significant positive correlation was seen with smoking duration in N0 VF (p=0.04, age‐adjusted). Current smokers (n=12) displayed higher N0 values than never (n=1) or ex‐smokers (n=11), 36.3±18.2 and 21.0±13.2, respectively (p< 0:01). No association was shown with occupational exposure.The present exploratory study suggests that assessment of individual lung cancer risk and susceptibility by the GPA assay does not seem to be feasible. The assay appears to provide a biomarker of longterm exposure to tobacco smoke.

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