Effects of Host Sex, Plant Species, and Putative Host Species on the Prevalence of Wolbachia in Natural Populations of Bemisia tabaci (Hemiptera: Aleyrodidae): A Modified Nested PCR Study

Effects of asexual reproduction as a primary reproductive strategy on population structure and levels of variability were investigated electrophoretically in natural populations of a woody plant species, trembling aspen (Populus tremuloides Michx.), from Alberta. As expected, levels of genic diversity, 42%, and proportion of polymorphic loci, 92%, averaged over all clones are considerably greater than those reported for comparable samples of sexually reproducing plant and animal species. These measures of genic variability of a primarily asexual plant species are similar to those reported for asexual species of insects, fish and bacteria. In addition, each of the 222 clones was electrophoretically unique. Since neutral theory would predict each individual clone to be heterozygous for a unique mutation at each gene locus at equilibrium, these results can be interpreted in a number of ways: (i) insufficient time to reach equilibrium, (ii) inability of electrophoresis to detect all variation at a locus, (iii) periodic establishment of sexually derived propagules in the population, and (iv) selection for similar genotypes at each location or against mutations at particular gene loci. Re-invasion of Pleistocene-glaciated areas by trembling aspen likely was by sexual means, with subsequent reproduction being primarily asexual.

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KISARAN INANG DAN DINAMIKA POPULASI Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) DI PERTANAMAN CABAI MERAH

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.11147-56 ◽

2011 ◽

Vol 11 (1) ◽

pp. 47-56

Author(s):

Hendrival Hendrival ◽

Purnama Hidayat ◽

Ali Nurmansyah

Keyword(s):

Host Range ◽

Host Plant ◽

Plant Species ◽

Bemisia Tabaci ◽

Population Dynamic ◽

Natural Enemy ◽

Host Plants ◽

Chili Pepper ◽

Plant Families ◽

Abundant Population

The study of host range and population dynamic of B. tabaci in red chili pepper fiel dswas conducted in Sub-district of Pakem, District of Sleman, Province of Daerah Istimewa Yogyakarta during dry season of May-October 2009. The study of host plants of B. tabaci from the red chili pepper fields revealed that there were 27 species of host plants belong to 22 genera of 13 families including crops and weeds. The host plants belong to families of Araceae, Amaranthaceae, Asteraceae, Brassicaceae, Capparidaceae, Convolvulaceae, Euphorbiaceae, Lamiaceae, Oxalidaceae, Papilionaceae, Rubiaceae, Solanaceae and Sterculiaceae. The host plant families of Asteraceae and Euphorbiaceae had the most abundant population of B. tabaci. Geminivirus-like symptoms were found in the weeds of A. conyzoides and A. boehmerioides. Population of B. tabaci adults correlated with abundance of host plant species found in the red chili pepper fields. The population of B. tabaci in red chili pepper fields was affected by natural enemy population. Population dynamic of the parasitoid Eretmocerus sp. correlated with population dynamic of the parasitized nymph of B. tabaci. Parasitoid Eretmocerus sp. was potentially good in controlling population of B. tabaci nymph in red chili pepper fields.

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Analysis of the genetic diversity of the coastal and island endangered plant species Elaeagnus macrophylla via conserved DNA-derived polymorphism marker

PeerJ ◽

10.7717/peerj.8498 ◽

2020 ◽

Vol 8 ◽

pp. e8498

Author(s):

Yi Wang ◽

Yan Ma ◽

Bingyu Jia ◽

Qichao Wu ◽

Dekui Zang ◽

...

Keyword(s):

Genetic Diversity ◽

Plant Species ◽

Diversity Index ◽

Geographic Distance ◽

Natural Populations ◽

Geographical Location ◽

Endangered Plant ◽

Endangered Plant Species ◽

Average Percentage ◽

Nanji Island

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant species Elaeagnus macrophylla were analyzed via conserved DNA-derived polymorphism molecular markers. A total of 289 discernible loci were obtained from 102 individuals via fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles was 1.9654, and the effective number of alleles was 1.2604. Nei’s genetic diversity index was 0.1724 on average, and Shannon’s information index was 0.2869, indicating that Elaeagnus macrophylla had lower levels of genetic diversity than those reported for its continental relatives and other continental species. The average percentage of polymorphic loci was 42.1%, and the maximum and minimum were 80.97% and 14.88%, respectively, which were associated with the Nanji Island and Liugong Island populations, respectively. The populations of Elaeagnus macrophylla were highly differentiated. Cluster analysis revealed that the similarity between the tested samples was related to their geographical location, that the samples from the same island tended to cluster together, and that there was no cross-clustering between samples. The Nanji Island and Da Rushan populations differentiated into two subpopulations. Last, we detected no correlation between genetic distance and geographic distance between populations (Pearson’s correlation coefficient r = 0.256579, p-value = 0.8309).

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Seasonal Variation in the Dietary Preferences of the Montane Vole, Microtus Montanus

The UW National Parks Service Research Station Annual Reports ◽

10.13001/uwnpsrc.1995.3253 ◽

1995 ◽

Vol 19 ◽

pp. 76-77

Author(s):

Aeltia Pinter ◽

Norman Negus ◽

Patricia Berger

Keyword(s):

Population Dynamics ◽

Seasonal Variation ◽

Plant Species ◽

Food Selection ◽

Natural Populations ◽

Food Preferences ◽

Plant Resources ◽

Microtus Montanus ◽

Dietary Preferences ◽

The One

Seasonal variation in food selection has been documented in several species on voles (Rothstein and Tamarin 1977, Cole and Batzli 1979 Goldberg et al. 1980) with considerable implications for winter survival and population dynamics. In Microtus Montanus a similar link may exist between growth, maturation, longevity, and population dynamics on the one hand and dietary composition on the other (e.g., Pinter and Negus 1965, Berger et al. 1981, Pinter 1988, Berger et al. 1992, Negus, Berger and Pinter 1992). Consequently, we undertook a study to investigate, in detail, the utilization of plant resources by the montane vole, Microtus montanus. The objectives of this project are twofold: (1) to identify the plant species that constitute the diet in natural populations of M. montanus and (2) to determine seasonal food preferences in relation to the availability of plant species and to the age, sex and cohorts of the montane vole.

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Development of retrotransposon-based SSAP molecular marker system for study of genetic diversity in sea holly (Eryngium maritimum L.)

Plant Genetic Resources ◽

10.1017/s1479262110000316 ◽

2010 ◽

Vol 8 (3) ◽

pp. 258-266 ◽

Cited By ~ 4

Author(s):

Baiba Ievina ◽

Naeem H. Syed ◽

Andrew J. Flavell ◽

Gederts Ievinsh ◽

Nils Rostoks

Keyword(s):

Genetic Diversity ◽

Molecular Marker ◽

Plant Species ◽

Natural Populations ◽

Wild Plant ◽

Northern Europe ◽

Marker System ◽

Northern Margin ◽

Wild Plant Species ◽

Eryngium Maritimum

Eryngium maritimum L. is a wild plant species threatened or endangered in most of Northern Europe, where species is on the northern margin of its distribution range. Recent studies have found reduction of size and even extinction of many populations. Assessment of genetic diversity in natural populations of endangered wild plant species can reflect condition and fitness of particular population and inform decisions on appropriate conservation measures. Application of inter simple sequence repeat markers and chloroplast DNA sequencing could not resolve genetic relationship between E. maritimum populations in Northern Europe. Therefore, the more sensitive retrotransposon-sequence-specific amplification polymorphism (SSAP) molecular marker system was developed. Six Ty1-copia long terminal repeat retrotransposons were isolated from E. maritimum genome (Tem1–Tem6) and assessed for their utility as molecular markers in this species. Two retrotransposons – Tem2 and Tem5 – were recognized as most informative based on the level of polymorphism and SSAP banding pattern quality. On average, 20.4% of SSAP bands were polymorphic for the five most informative primer combinations in a set of 150 Northern European E. maritimum plants from 13 locations, providing a useful tool for assessment of genetic diversity in this endangered species.

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