Ultrastructure and behavior of actin cytoskeleton during cell wall formation in the fission yeast Schizosaccharomyces pombe

2003 ◽  
Vol 52 (>2) ◽  
pp. 161-174 ◽  
Author(s):  
T. Takagi
Keyword(s):  
Cell Wall ◽  
Actin Cytoskeleton ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Cell Wall Formation ◽  
Wall Formation ◽  
And Behavior

Dynamics of Cell Wall Formation in Fission Yeast,Schizosaccharomyces pombe

1998 ◽  
Vol 24 (1-2) ◽  
pp. 178-206 ◽  
Author(s):  
Masako Osumi ◽  
Mamiko Sato ◽  
Sanae A Ishijima ◽  
Mami Konomi ◽  
Tomoko Takagi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Cell Wall Formation ◽  
Wall Formation

Actin is associated with the formation of the cell wall in reverting protoplasts of the fission yeast Schizosaccharomyces pombe

1989 ◽  
Vol 94 (4) ◽  
pp. 635-646
Author(s):  
H. Kobori ◽  
N. Yamada ◽  
A. Taki ◽  
M. Osumi
Keyword(s):  
Cell Wall ◽  
Schizosaccharomyces Pombe ◽  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
High Dose ◽  
Cell Wall Formation ◽  
High Concentration ◽  
Wall Formation ◽  
Close Relationship ◽  
Fibrillar Network

To clarify the involvement of actin in the formation of the yeast cell wall, reverting protoplasts of Schizosaccharomyces pombe were used as a simple model system. Actin of reverting protoplasts was labeled with rhodamine-conjugated phalloidin and observed by conventional fluorescence microscopy and laser scanning confocal microscopy. A close spatial as well as temporal relationship between actin and cell wall formation was observed in protoplast reversion. That is, the site of actin ‘dots’ in the reverting protoplasts coincided with the site of new wall formation and the timing of rearrangement of actin coincided with the initiation of cell wall formation and with the timing of cell wall expansion. Treatment of reverting protoplasts with cytochalasin D (CD) further clarified the close relationship between actin and cell wall organization. The effect of CD was dose dependent. A high dose of CD caused the absence of actin as well as the complete inhibition of cell wall formation. A low dose of CD caused weakly stained unlocalized actin, which induced grossly aberrant cell wall deposition as well as substantial changes in the morphology of the reverting protoplasts. These results demonstrated that actin is associated with initiation of cell wall formation, the proper deposition of cell wall materials, and maintaining the normal morphology of reverting protoplasts. Scanning electron microscopy revealed the presence of a fibrillar net structure on the surface of non-treated control reverting protoplasts. However, the absence of a fibrillar network on the surface of reverting protoplasts was observed in the presence of a high concentration of CD. Lack of localization of microfibrils as well as poor development of the fibrillar network were also observed in the presence of a low concentration of CD. Recovery experiments confirmed the close relationship between actin and cell wall formation.

Fission yeast protein kinase C gene homologues are required for protoplast regeneration: a functional link between cell wall formation and cell shape control

1994 ◽  
Vol 107 (5) ◽  
pp. 1131-1136 ◽  
Author(s):  
H. Kobori ◽  
T. Toda ◽  
H. Yaguchi ◽  
M. Toya ◽  
M. Yanagida ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fission Yeast ◽  
Wild Type Strain ◽  
Protoplast Regeneration ◽  
Cell Wall Formation ◽  
Wild Type ◽  
Intact Cells ◽  
Actin Organization ◽  
Wall Formation ◽  
Kinase C

Two novel protein kinase C (n PKC) gene homologues, pck1+ and pck2+ were isolated from the fission yeast Schizosaccharomyces pombe (Toda et al. (1993) EMBO J. 12, 1987). We examined the functional differences of pck1+ and pck2+ in cell wall formation and actin organization of S. pombe. Regenerating protoplasts of a wild-type strain, single gene disruptants of pck1+ (delta pck1) and pck2+ (delta pck2) were used as a simple model to examine the functional links between PKC, cell wall formation and actin organization. Protoplasts of the wild-type strain and those of delta pck1 reverted to intact cells in osmotically stabilized liquid medium. A close spatial association between new cell wall formation and actin was observed in these two strains. In delta pck2, protoplasts did not revert to intact cells: (1) scarcely any new cell wall material was formed; (2) actin was not reorganized; and (3) nuclear division and an increase in the amount of cytoplasm were observed in the regenerating protoplasts. These findings demonstrate that the pck2+ gene has a function essential for protoplast regeneration but the pck1+ gene does not. Involvement of n PKCs in cell wall formation and actin organization was also clarified. The effect of staurosporine (a potent inhibitor of protein kinases) on regenerating protoplasts of the three strains confirmed the assumption that the pck2 protein is an in vivo target of staurosporine in the fission yeast.

Ethylene effects on cambial activity and cell wall formation in hypocotyls of Picea abies seedlings

1991 ◽  
Vol 82 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Barbro S. M. Ingemarsson ◽  
Leif Eklund ◽  
Lennart Eliasson
Keyword(s):  
Cell Wall ◽  
Picea Abies ◽  
Cambial Activity ◽  
Cell Wall Formation ◽  
Wall Formation

Arabidopsis Callose Synthase Gene GSL8 is Required for Cell Wall Formation and Establishment and Maintenance of Quiescent Center

2014 ◽  
Vol 48 (4) ◽  
pp. 389-397
Author(s):  
Liu Lin ◽  
Quan Xianqing ◽  
Zhao Xiaomei ◽  
Huang Lihua ◽  
Feng Shangcai ◽  
...  
Keyword(s):  
Cell Wall ◽  
Quiescent Center ◽  
Cell Wall Formation ◽  
Callose Synthase ◽  
Wall Formation

scholarly journals MYB Transcription Factors and Its Regulation in Secondary Cell Wall Formation and Lignin Biosynthesis during Xylem Development

2021 ◽  
Vol 22 (7) ◽  
pp. 3560
Author(s):  
Ruixue Xiao ◽  
Chong Zhang ◽  
Xiaorui Guo ◽  
Hui Li ◽  
Hai Lu
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Secondary Wall ◽  
Secondary Cell Wall ◽  
Lignin Biosynthesis ◽  
Cell Wall Formation ◽  
Long Distance ◽  
Secondary Cell Wall Formation ◽  
Myb Transcription Factors ◽  
Wall Formation

The secondary wall is the main part of wood and is composed of cellulose, xylan, lignin, and small amounts of structural proteins and enzymes. Lignin molecules can interact directly or indirectly with cellulose, xylan and other polysaccharide molecules in the cell wall, increasing the mechanical strength and hydrophobicity of plant cells and tissues and facilitating the long-distance transportation of water in plants. MYBs (v-myb avian myeloblastosis viral oncogene homolog) belong to one of the largest superfamilies of transcription factors, the members of which regulate secondary cell-wall formation by promoting/inhibiting the biosynthesis of lignin, cellulose, and xylan. Among them, MYB46 and MYB83, which comprise the second layer of the main switch of secondary cell-wall biosynthesis, coordinate upstream and downstream secondary wall synthesis-related transcription factors. In addition, MYB transcription factors other than MYB46/83, as well as noncoding RNAs, hormones, and other factors, interact with one another to regulate the biosynthesis of the secondary wall. Here, we discuss the biosynthesis of secondary wall, classification and functions of MYB transcription factors and their regulation of lignin polymerization and secondary cell-wall formation during wood formation.

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