Contrasting dynamics in abscisic acid metabolism in different Fragaria spp. during fruit ripening and identification of involved enzymes

Abstract Abscisic acid (ABA) is a key hormone in non-climacteric Fragaria spp, regulating multiple physiological processes throughout fruit ripening. Its level increases during ripening, and it promotes fruit (receptacle) development. However, its metabolism in the fruit is largely unknown. We analyzed the levels of ABA and its catabolites at different developmental stages of strawberry ripening in diploid and octoploid genotypes and identified two functional ABA-glucosyltransferases (FvUGT71A49 and FvUGT73AC3) and two regiospecific ABA-8’-hydroxylases (FaCYP707A4a and FaCYP707A1/3). ABA-glucose-ester content increased during ripening in diploid F. vesca varieties but decreased in octoploid F. xananassa. Dihydrophaseic acid content increased throughout ripening in all analyzed receptacles, while 7’-hydroxy-ABA and neo-phaseic acid did not show significant changes during ripening. In the studied F. vesca varieties, the receptacle seems to be the main tissue for ABA metabolism, as the content of ABA and its metabolites in the receptacle was generally 100 times higher than in achenes, respectively. The accumulation patterns of ABA catabolites and transcriptomic data from the literature show that all strawberry fruits produce and metabolize considerable amounts of the plant hormone ABA during ripening, which is therefore a conserved process, but also illustrate the diversity of this metabolic pathway which is species, variety and tissue dependent.

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Abscisic Acid Metabolism during Fruit Development and Maturation of Mangosteens

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.127.5.737 ◽

2002 ◽

Vol 127 (5) ◽

pp. 737-741 ◽

Cited By ~ 8

Author(s):

Satoru Kondo ◽

Wanvisa Ponrod ◽

Sirichai Kanlayanarat ◽

Nobuhiro Hirai

Keyword(s):

Abscisic Acid ◽

Fruit Development ◽

Acid Metabolism ◽

Phaseic Acid ◽

Garcinia Mangostana ◽

Aba Metabolism ◽

Dihydrophaseic Acid ◽

Main Pathway ◽

Abscisic Acid Metabolism ◽

Nonclimacteric Fruit

Endogenous abscisic acid (ABA), its 2-trans isomer (trans-ABA), phaseic acid (PA), and dihydrophaseic acid (DPA) concentrations were quantified in the peel, aril, and seed of mangosteen (Garcinia mangostana L.). Changes in carbon dioxide (CO2) and ethylene (C2H4) production and 1-aminocyclopropane-1-carboxylic acid (ACC) concentration in the peel and aril were also examined. ACC concentration and CO2 and C2H4 production were high at the beginning of fruit development and gradually decreased toward harvest, which confirms that mangosteen is a nonclimacteric fruit. In the peel and aril, the increase in ABA concentration preceded the decrease in peel firmness and coloring of the peel. This suggests that ABA may induce the maturation of mangosteens. The state of ABA metabolism varied with the part of fruit. In the peel, PA and DPA were not considered to be predominant metabolites of ABA because their concentrations were low compared to ABA throughout fruit development. In contrast, in the aril and seed, it is possible that the PA-DPA pathway may be a main pathway of ABA metabolism because the concentrations of DPA in the aril and of PA in the seed directly coincided with the concentrations of ABA. The differences in the ABA metabolites between aril and seed may be caused by the rate of ABA metabolism. The concentrations of ABA and its metabolite in the seed decreased toward harvest.

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NAC transcription factors SNAC4 and SNAC9 synergistically regulate tomato fruit ripening by affecting expression of genes involved in ethylene and abscisic acid metabolism and signal transduction

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2021.111555 ◽

2021 ◽

Vol 178 ◽

pp. 111555

Author(s):

Sen Yang ◽

Jiaqian Zhou ◽

Christopher B. Watkins ◽

Caie Wu ◽

Yanchun Feng ◽

...

Keyword(s):

Signal Transduction ◽

Transcription Factors ◽

Abscisic Acid ◽

Fruit Ripening ◽

Acid Metabolism ◽

Tomato Fruit ◽

Nac Transcription Factors ◽

Expression Of Genes ◽

Tomato Fruit Ripening ◽

Abscisic Acid Metabolism

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Differential expression of abscisic acid metabolism and signalling genes induced by seed-covering structures or hypoxia in barley (Hordeum vulgare L.) grains

Seed Science Research ◽

10.1017/s0960258509990262 ◽

2010 ◽

Vol 20 (2) ◽

pp. 69-77 ◽

Cited By ~ 14

Author(s):

Guillermina M. Mendiondo ◽

Juliette Leymarie ◽

Jill M. Farrant ◽

Françoise Corbineau ◽

Roberto L. Benech-Arnold

Keyword(s):

Abscisic Acid ◽

Candidate Genes ◽

Oxygen Supply ◽

Acid Metabolism ◽

Physiological Responses ◽

Hordeum Vulgare L ◽

Aba Metabolism ◽

Expression Of Genes ◽

Abscisic Acid Metabolism ◽

Barley Grains

AbstractDormant barley grains cannot germinate at 30°C and this inability to germinate is imposed mostly by the glumellae which have been suggested to limit oxygen supply to the embryo. Hypoxia imposed either artificially or by the glumellae to embryos from dormant grains, increases embryo sensitivity to abscisic acid (ABA) and promotes the accumulation of ABA during the first hours after imbibition. Expression of candidate genes involved in ABA synthesis (HvNCED), catabolism (HvABA8OH1) and signalling (HvABI5, HvVP1 and HvPKABA) was analysed in embryos isolated from dormant whole or de-hulled grains incubated in air or in hypoxia (5% oxygen). The presence of the glumellae enhanced the expression of genes involved in ABA metabolism and signalling with respect to that observed in de-hulled grains incubated in air. These results suggest that at least part of the observed physiological responses to the presence of the glumellae are regulated at the level of gene expression. However, hypoxia imposed on dormant de-hulled grains did not mimic the presence of the glumellae in terms of expression of candidate genes. Hypoxia mimics the presence of the glumellae in terms of dormancy maintenance and ABA accumulation and sensitivity, but its effects appear to operate through different mechanisms.

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