FP587ASSOCIATION OF BASELINE LEVELS OF PRO-INFLAMMATORY CYTOKINES WITH THE NEAR 7-YEAR MORTALITY RATE IN INCIDENT PERITONEAL DIALYSIS PATIENTS

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii269-iii269
Author(s):  
Yao-Lung Liu ◽  
Shih-Yi Lin ◽  
Jiung-Hsiun Liu ◽  
I-Ru Chen ◽  
I-Kuan Wang ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Mortality Rate ◽  
Inflammatory Cytokines ◽  
Dialysis Patients ◽  
Baseline Levels ◽  
Pro Inflammatory Cytokines

scholarly journals Pro-inflammatory cytokines: a possible relationship with dialytic adequacy and serum albumin in peritoneal dialysis patients

2015 ◽  
Vol 9 (1) ◽  
pp. 153-157 ◽  
Author(s):  
Sabrina Milan Manani ◽  
Grazia Maria Virzì ◽  
Anna Clementi ◽  
Alessandra Brocca ◽  
Massimo de Cal ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Serum Albumin ◽  
Inflammatory Cytokines ◽  
Dialysis Patients ◽  
Pro Inflammatory Cytokines

scholarly journals A Novel Formulation of Glucose-Sparing Peritoneal Dialysis Solutions with l-Carnitine Improves Biocompatibility on Human Mesothelial Cells

2020 ◽  
Vol 22 (1) ◽  
pp. 123
Author(s):  
Francesca Piccapane ◽  
Mario Bonomini ◽  
Giuseppe Castellano ◽  
Andrea Gerbino ◽  
Monica Carmosino ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Cell Viability ◽  
Inflammatory Cytokines ◽  
Mesothelial Cells ◽  
Apical Side ◽  
New Formulation ◽  
Stage Renal Disease ◽  
End Stage ◽  
Dialysis Solutions ◽  
Pro Inflammatory Cytokines

The main reason why peritoneal dialysis (PD) still has limited use in the management of patients with end-stage renal disease (ESRD) lies in the fact that the currently used glucose-based PD solutions are not completely biocompatible and determine, over time, the degeneration of the peritoneal membrane (PM) and consequent loss of ultrafiltration (UF). Here we evaluated the biocompatibility of a novel formulation of dialytic solutions, in which a substantial amount of glucose is replaced by two osmometabolic agents, xylitol and l-carnitine. The effect of this novel formulation on cell viability, the integrity of the mesothelial barrier and secretion of pro-inflammatory cytokines was evaluated on human mesothelial cells grown on cell culture inserts and exposed to the PD solution only at the apical side, mimicking the condition of a PD dwell. The results were compared to those obtained after exposure to a panel of dialytic solutions commonly used in clinical practice. We report here compelling evidence that this novel formulation shows better performance in terms of higher cell viability, better preservation of the integrity of the mesothelial layer and reduced release of pro-inflammatory cytokines. This new formulation could represent a step forward towards obtaining PD solutions with high biocompatibility.

scholarly journals A comparison of mortality between patients treated with hemodialysis and peritoneal dialysis.

1995 ◽  
Vol 6 (2) ◽  
pp. 177-183
Author(s):  
W E Bloembergen ◽  
F K Port ◽  
E A Mauger ◽  
R A Wolfe
Keyword(s):  
Peritoneal Dialysis ◽  
Mortality Rate ◽  
High Mortality ◽  
Epidemiologic Study ◽  
National Sample ◽  
National Study ◽  
Dialysis Patients ◽  
All Cause Mortality ◽  
Males And Females

Patients with ESRD treated with dialysis have a high mortality rate. Controversy exists as to whether this high mortality rate is affected by modality choice. The purpose of this epidemiologic study was to compare mortality in prevalent hemodialysis-treated (HD) and peritoneal dialysis-treated (PD) patients in a large national sample, adjusting for demographic characteristics. Data were obtained from the U.S. Renal Data System for patients prevalent on January 1 of the years 1987, 1988, and 1989, each with 1 yr of follow-up. Patients were censored at transplantation. Death rates per 100 patient years were compared between HD and PD, adjusting for age, race, gender, cause of ESRD (diabetes versus nondiabetes) and < 1 yr or > 1 yr of prior ESRD, by the use of Poisson regression. There were 42,372 deaths occurring over 170,700 patient years at risk. On average, prevalent patients treated with PD had a 19% higher adjusted mortality risk (relative risk (RR) = 1.19; P < 0.001) than did those treated with HD. This risk was found to be insignificant (P > 0.05) and small for ages < 55 and increasingly large and significant for ages > 55 yr. It was accentuated in diabetics (RR = 1.38; P < 0.001) but was also present in nondiabetics (RR = 1.11; P < 0.001). Although present in both males and females, this risk was accentuated in females (RR = 1.30 versus 1.11; both P < 0.001). In this national study of prevalent U.S. dialysis patients, treatment assignment to PD was associated with a 19% higher all-cause mortality rate than HD.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals P1130A CELLULAR MODEL OF HUMAN MESOTHELIUM TO STUDY OF WATER TRANSPORT DURING PERITONEAL DIALYSIS AND THE BIOCOMPATIBILITY OF INNOVATIVE GLUCOSE-SPARING SOLUTIONS.

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Francesca Piccapane ◽  
Rosa Caroppo ◽  
Arduino Arduini ◽  
Roberto Corciulo ◽  
Roberto Russo ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Water Transport ◽  
Inflammatory Cytokines ◽  
Glucose Concentration ◽  
Water Channel ◽  
Mesothelial Cells ◽  
Dependent Manner ◽  
Endogenous Expression ◽  
Apical Side ◽  
Pro Inflammatory Cytokines

Abstract Background and Aims The three pore model postulates that the endothelium of peritoneal capillaries is the major limiting barrier regulating water transport across peritoneal membrane during peritoneal dialysis (PD). We hypothesize that the mesothelium may represent an additional selective barrier to water diffusion in PD. We previously demonstrated that the water channel AQP1 is expressed in vivo by mesothelial cells. Here, we characterized an immortalized cell line of human mesothelium (HMC) to study the functional role of the water channel AQP1 in mediating water transport during PD and also to test the biocompatibility of glucose-sparing PD solutions (Xylocore), containing xylitol and L-carnitine as the main osmotic agents. Method Cells were grown onto porous cell culture inserts to achieve polarization. Polarization was demonstrated by expression of the tight junction markers Zo-1 and occludin. Transepithelial water transport was measured by TEA+-sensitive microelectrodes. HMC cell monolayers were exposed to PD solutions at the apical side for 8 hours. The biocompatibility of conventional versus innovative PD solutions was evaluated by MTT-test, measurement of transepithelial electrical resistance (TEER) and production of pro-inflammatory cytokines by by Luminex xMAP technology. Results HMC cells showed polarized expression of Na+/K+-ATPase and tight junctions markers but no endogenous expression of AQP1. HMC showed a low TEER (40Ω/cm2) compared to renal cells not expressing AQP1(1000Ω/cm2). However, the transepithelial water transport was comparable between the two cell types. Experiments in HMCs transfected with AQP1 cDNA, suggested that the water permeability of HMC was increased by two-fold in the presence of AQP1. Biocompatibility assays indicated that in conventional dialysis solutions glucose concentration decreased cell viability in a dose-dependent manner. Glucose concentration also strongly decreased the TEER, suggesting reduction of the barrier integrity, and increased pro-inflammatory cytokines production. Interestingly, substitution of part of the glucose with xylitol and L-carnitine minimized these effects. Conclusion These results suggest that the mesothelium may represent an additional selective barrier regulating water transport through the water channel AQP1 in PD. Importantly, we also demonstrate that the formulation of glucose-sparing PD solutions containing xylitol and L-carnitine better preserve mesothelial cells viability and may represent a useful means to prolong the dialysis life of patients undergoing peritoneal dialysis.

scholarly journals Association of inflammatory cytokines with mortality in peritoneal dialysis patients

BioMedicine ◽  
2017 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Yao-Lung Liu ◽  
Jiung-Hsiun Liu ◽  
I-Kuan Wang ◽  
Shu-Woei Ju ◽  
Tung-Min Yu ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Inflammatory Cytokines ◽  
Dialysis Patients

P5511Markers of vascular damage in cardiovascular events prediction: results from cluster analysis

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
A R Babaeva ◽  
A A Tarasov ◽  
S I Davidov ◽  
M A Gordeeva ◽  
E A Reznikova ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Connective Tissue ◽  
Inflammatory Cytokines ◽  
Clinical Manifestation ◽  
Cardiovascular Events ◽  
Low Grade ◽  
Tnf Α ◽  
Circulating Markers ◽  
Baseline Levels ◽  
Pro Inflammatory Cytokines

Abstract Background As a low grade systemic inflammation plays an important role in the pathogenesis of atherosclerosis-associated diseases and diabetes mellitus (DM) there is an interest in the relevance of circulating markers of immune inflammation to clinical manifestation of cardiovascular disease (CVD), especially in the setting of DM. Purpose Purpose of our investigation was to assess the predictive value of numerous immune markers (pro-inflammatory cytokines, anti-connective tissue antibodies) in relation with circulating markers of endothelial dysfunction (ED) in persons with documented ischemic heart disease (IHD), DM, and asymptomatic atherosclerosis (AA). Methods 393 persons (147 pts with IHD, 126 pts with T2DM, 120 pts with AA) were observed during 3-year period. The baseline levels of pro-inflammatory cytokines (IL1β, IL6, TNF-α), antibodies against connective tissue components (collagen – antiC-ab, hyaluronic acid – antiHA-ab, chondroitin sulfate antiCS-ab), soluble markers of ED: von Willebrand factor (vWf), endothelin 1 (ET-1), endogenous NO synthase (eNOs) were evaluated by ELISA. The incidence and severity of cardiovascular events (CVE) in the relation with baseline levels of measured markers were evaluated by cluster analysis in 4 cohorts formed according to presence of AA, current IHD and T2DM (AA, IHD, T2DM, IHD+T2DM). From 2 to 4 clusters were separated depending on the incidence and severity of CVE. Results We have defined that in AA the numerous of circulating markers: ET-1, IL-1β, TNFα, antiC-ab, antiCS-ab was associated with clinically significant CVE. In IHD the most severe clinical manifestations were documented in cluster, characterized by increased ET1, vWf, IL6, antiC-ab levels and decreased eNOs, In T2DM without evidenced IHD CVE were associated with next profile: ET1, eNOs, IL-6, antiC-ab, and antiHA-ab. In combination of IHD with T2DM the worst cluster was presented with raised levels of vWf, TNF-α, IL-6, antiC-ab, anti-HA and CRP as well as decreased eNOs. Conclusions Circulating markers of ED and immune-mediated inflammation reflect the clinical manifestation of IHD in high-risk persons with AA and T2DM. Cluster analysis has demonstrated the relationship between specific baseline profile of investigated biomarkers and clinical significant CVE. Obtained data broads our understanding regarding the inflammatory mechanism of atherosclerosis and also suggest a set of circulating markers as predictors of adverse cardiovascular events.

Sign in / Sign up

Export Citation Format

Share Document