Low-voltage backscattered electron imaging of non-coated biological samples in a low-vacuum environment using a variable-pressure scanning electron microscope with a YAG-detector

1998 ◽  
Vol 47 (4) ◽  
pp. 351-354 ◽  
Author(s):  
T. Ushiki ◽  
H. Hashizume ◽  
S. Itoh ◽  
K. Kuboki ◽  
S. Saito ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Biological Samples ◽  
Low Voltage ◽  
Variable Pressure ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Scanning Electron

Backscattered Electron Imaging in the Scanning Electron Microscope: the Use of Either: (a) High Incident Energy or (b) an Array Detector

2008 ◽  
Vol 14 (S2) ◽  
pp. 120-121
Author(s):  
LM Gignac ◽  
OC Wells ◽  
C-K Hu ◽  
J Bruley ◽  
CE Murray ◽  
...  
Keyword(s):  
Electron Microscope ◽  
New Mexico ◽  
Scanning Electron Microscope ◽  
Incident Energy ◽  
Array Detector ◽  
Backscattered Electron Imaging ◽  
High Incident Energy ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

scholarly journals Simultaneous visualization of myeloperoxidase reactivity and immunogold labeling by backscattered electron imaging with the scanning electron microscope.

1987 ◽  
Vol 35 (2) ◽  
pp. 267-269 ◽  
Author(s):  
D Soligo ◽  
E P de harven
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Cell Types ◽  
Myeloperoxidase Activity ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Simultaneous Visualization ◽  
Scanning Electron

We describe a method to label circulating human granulocytes with an immunogold marker and then incubate them for demonstration of myeloperoxidase activity. The cells were observed in the backscattered electron imaging (BEI) mode of the scanning electron microscope. This permits simultaneous visualization of cell surface morphology, the immunological surface marker, and the cytochemical reactivity of each individual cell. Enhanced identification of the various cell types and more precise characterization of cell surface features in the different steps of leukocyte differentiation are expected to result from application of this technique.

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