Backscattered electron imaging for immunogold viewing by scanning electron microscope

1984 ◽  
Vol 70 (2) ◽  
pp. 269-271 ◽  
Author(s):  
M.T. Nava ◽  
D. Soligo ◽  
E. Pozzoli ◽  
G. Lambertenghi-Deliliers
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Scanning Electron

Backscattered Electron Imaging in the Scanning Electron Microscope: the Use of Either: (a) High Incident Energy or (b) an Array Detector

2008 ◽  
Vol 14 (S2) ◽  
pp. 120-121
Author(s):  
LM Gignac ◽  
OC Wells ◽  
C-K Hu ◽  
J Bruley ◽  
CE Murray ◽  
...  
Keyword(s):  
Electron Microscope ◽  
New Mexico ◽  
Scanning Electron Microscope ◽  
Incident Energy ◽  
Array Detector ◽  
Backscattered Electron Imaging ◽  
High Incident Energy ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

scholarly journals Simultaneous visualization of myeloperoxidase reactivity and immunogold labeling by backscattered electron imaging with the scanning electron microscope.

1987 ◽  
Vol 35 (2) ◽  
pp. 267-269 ◽  
Author(s):  
D Soligo ◽  
E P de harven
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Cell Types ◽  
Myeloperoxidase Activity ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Simultaneous Visualization ◽  
Scanning Electron

We describe a method to label circulating human granulocytes with an immunogold marker and then incubate them for demonstration of myeloperoxidase activity. The cells were observed in the backscattered electron imaging (BEI) mode of the scanning electron microscope. This permits simultaneous visualization of cell surface morphology, the immunological surface marker, and the cytochemical reactivity of each individual cell. Enhanced identification of the various cell types and more precise characterization of cell surface features in the different steps of leukocyte differentiation are expected to result from application of this technique.

Backscattered electron imaging as a tool for histochemically localizing calcium oxalate with the scanning electron microscope

1987 ◽  
Vol 65 (5) ◽  
pp. 888-892 ◽  
Author(s):  
J. A. Traquair
Keyword(s):  
Acetic Acid ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Calcium Oxalate ◽  
Leaf Tissues ◽  
Backscattered Electron ◽  
Staining Techniques ◽  
Electron Imaging ◽  
First Time ◽  
Scanning Electron

The scanning electron microscope and a backscattered electron detector were used for the first time to identify calcium oxalate in leaf tissues after labelling crystals with silver. Freehand sections fixed in formalin – acetic acid – alcohol or glutaraldehyde were treated with 5% acetic acid to remove background calcium carbonate and calcium phosphate and were silver stained by calcium-substitution techniques. Two standard staining techniques, Pizzolato (AgNO3–H2O2) and Yasue (AgNO3 – rubeanic acid) tests, were compared. Druses and raphides were identified morphologically and histochemically in the leaves of Anthurium scherzeranum and Dieffenbachia maculata. The Pizzolato procedure was the best method for staining calcium oxalate for scanning electron microscope studies. Excessive deposition of granular material in the Yasue staining method tended to obscure crystal morphology in scanning electron microscope preparations.

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