Vitronectin (VTN): A Novel Diagnostic and Prognostic Marker for Hepatocellular Carcinoma (HCC) On Top Of Chronic Hepatitis C Virus Related Diseases

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Amal S Bakir ◽  
Hesham H Al-Kilany ◽  
Ramy S Ghait ◽  
Hamed S Badawy
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Study Groups ◽  
Diagnosis And Prognosis ◽  
Group 4 ◽  
Significant Difference ◽  
Median Level ◽  
Cirrhotic Patients ◽  
Group 3

Abstract Background Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and the most common form of primary liver cancer. Aim of the study to study the level of serum vitronectin (VTN) compared to AFP in diagnosis and prognosis of hepatocellular carcinoma on top of HCV-related liver diseases. Patients and Methods This prospective observational study included a total number of 60 subjects who were divided into 4 groups: Group 1: include 10 normal persons. Group 2: included 10 patients who have Hepatitis C Virus infection. Group 3: included 20 cirrhotic patients. Group 4: included 20 patients with HCC (on top of hepatitis C virus related cirrhosis), in which we tested them for vitronectin before first intervention and after intervention within 3 months. Results The mean age of the cases showed high statistically significant difference between the study groups (P < 0.001).There were a total number of 29 males and 31 females with significant difference in the sex distribution between the study groups. 80% of the cases in HCC group were males. This sex distribution can be attributed to high prevalence of risk factors like smoking, DM and HCV in males in addition to possible role of sex hormones. On analyzing laboratory characters of the studied groups, we found statistically significant difference between cirrhosis group and non-cirrhotic groups with low platelets count, high serum creatinine, high INR, lower serum albumin, high bilirubin and higher AST. The median level of AFP in group 4 (HCC patients) was 110 IU/ml which was higher than its median value in the other study groups with high level of significance between the study groups. The median level of vitronectin levels in the different study groups didn’t reveal a statistically significant difference between the different study groups. Serum level of vitronectin in group 3 (cirrhotic patients) has no statistically significant difference between the three subgroups according to child's classification. By the analysis of serum level of AFP and vitronectin level in group IV (HCC patients) before and after treatment, the median level of AFP was significantly lower than its level before intervention, while the decrease in vitronectin level was statistically insignificant after intervention. Conclusions Chronic HCV infection and its serious complicatons specially HCC are still representing a health challenge in Egypt, reflecting wide HCV prevalence and late diagnosis. Serum AFP alone has an unreliable role in HCC surveillance as it has low sensitivity because it may be normal in up to 40% of HCC cases especially early stages of the tumor, and low specificity as its levels may be elevated in conditions other than HCC as cirrhosis or exacerbation of chronic hepatitis or even in some cases of cholangiocarcinoma. AFP is useful in clinical practice in screening and diagnosis of HCC in association with US and CT. According to our study, we can't use Vitronectin alone in diagnosis and prognosis of HCC on top of cirrhosis related to HCV +ve infection. It didn’t reveal a statistically significant difference between the different study groups ,so it may require further study and research.

scholarly journals Hepatocellular carcinoma in patients undergoing orthotopic liver transplantation: radiological findings with anatomopathological correlation in Brazil

2006 ◽  
Vol 43 (1) ◽  
pp. 24-29 ◽  
Author(s):  
Antônio Carlos Maciel ◽  
Carlos Thadeu Cerski ◽  
Roger Klein Moreira ◽  
Vinicius Labrea Resende ◽  
Maria Lúcia Zanotelli ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Hepatocellular Carcinoma ◽  
Liver Transplantation ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Orthotopic Liver Transplantation ◽  
Imaging Techniques ◽  
Arterial Phase ◽  
Hepatitis C Virus Infection ◽  
Cirrhotic Patients

BACKGROUND: Hepatocellular carcinoma is one of the most common malignant tumors worldwide. Imaging techniques, specially computed tomography and ultrasound, are among the most useful diagnostic tools, although the accuracy of these methods may have a significant variability. AIMS: To determine the prevalence of hepatocellular carcinoma in cirrhotic patients undergoing orthotopic liver transplantation at "Santa Casa de Misericórdia" of Porto Alegre, RS, Brazil; to estimate the sensitivity of computed tomography and ultrasound in pretransplantation detection of hepatocellular carcinoma in this population; to correlate the radiological characteristics with anatomopathological findings. MATERIALS AND METHODS: Retrospective prevalence study. Population: adult, cirrhotic patients undergoing orthotopic liver transplantation from January 1990 to July 2003. Among the 292 transplanted patients, 31 cases of hepatocellular carcinoma were diagnosed, of which 29 were included in the study. Tumor characteristics in both ultrasound and computed tomography were compared to those observed in anatomopathological examination. RESULTS: Prevalence of hepatitis C virus infection among patients with diagnosis of hepatocellular carcinoma was 93.5%, and the prevalence of hepatocellular carcinoma among transplanted patients was 10.6%. The overall sensitivity of the imaging techniques was 70.3% for computed tomography and 72% for ultrasound. CONCLUSION: The prevalence of hepatocellular carcinoma at our institution, as well as the sensitivity of both ultrasound and computed tomography to detect such tumors at pretransplantation screening were similar to those found by other authors, while the prevalence of hepatitis C virus infection, the most common etiological agent for liver disease in our patients, is one of the highest ever reported in literature. Factors influencing hepatocellular carcinoma detection rates were: time from examination to liver transplantation; acquisition of computed tomography images during arterial phase; lesion size. Arterial phase proved to be the most useful part of computed tomography examination in this study.

scholarly journals A potential clinical based score in hepatitis C virus cirrhotic patients to exclude small hepatocellular carcinoma

2018 ◽  
Vol 4 (4) ◽  
pp. 11
Author(s):  
Denise Cerqueira Paranaguá-Vezozzo ◽  
Celso Eduardo Lourenço Matielo ◽  
Daniel Ferraz De Campos Mazo ◽  
Lucas Souto Nacif ◽  
Mario Guimaraes Pessoa ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Small Hepatocellular Carcinoma ◽  
Cirrhotic Patients

scholarly journals A Comparative Study of the Diagnostic and Prognostic Value of Macrophage Activation Marker Soluble CD163 and Alpha Fetoprotein in Cirrhotic Patients with Hepatitis C Virus-related Hepatocellular Carcinoma treated by Loco-Regional Therapy

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
K A Mohamed ◽  
E S Mohamed ◽  
A M Hussein ◽  
M H A Fouad ◽  
A S Allam ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Prognostic Marker ◽  
Prognostic Value ◽  
Macrophage Activation ◽  
Activation Marker ◽  
Soluble Cd163 ◽  
Cirrhotic Patients

Abstract Background hepatocellular carcinoma (HCC) shows an increasing incidence and represents the third most common cause of cancer-related death. Aim of the Work is to evaluate the diagnostic value of serum level of Macrophage activation marker soluble CD163 as a tumor marker for HCC and its prognostic value after transarterial chemo-embolization (TACE) or radiofrequency ablation (RFA), in comparison to alpha-feto protein (AFP). Patients and Methods this study was performed on 60 subjects from the outpatient Hepatology clinic and inpatient Gastroenterology and Hepatology Department at Ain Shams University Hospital. Group I includes 40 randomly selected cirrhotic patients with hepatitis C virus-related hepatocellular carcinoma (excluding BCLC class D) who underwent either RFA or TACE. Group II includes 20 patients with liver cirrhosis without hepatocellular carcinoma considered as control. Results soluble CD163 expression did not differ significantly between HCC group and liver cirrhosis group. This proves that soluble CD163 is not suitable for diagnostic use. On the other hand, soluble CD163 was associated with the severity of liver disease. Baseline soluble CD163 was significantly associated with disease progression independent of other risk factors known to be associated with an unfavorable course in HCC. Also the marked significant reduction of serum soluble CD163 levels in HCC patients subjected to either RF ablation or TACE proved that soluble CD163 may play a prognostic marker in HCC monitoring. Conclusion soluble CD163 is not suitable as a diagnostic marker for HCC but can be used as a prognostic marker for HCC.

MicroRNA-30e and MicroRNA-223 Expression for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C Virus

2020 ◽  
Vol 3 (1) ◽  
pp. 97-104
Author(s):  
Amany Ragab Youssef ◽  
Samah Bastawy ◽  
Karim Montasser ◽  
Sally Abed ◽  
Muhammad Diasty ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Early Diagnosis ◽  
Early Stage ◽  
Control Subjects ◽  
Combined Use ◽  
Significant Difference ◽  
Early Hcc ◽  
Healthy Control

Background. The goals of this study were to elucidate the use of the expression of microRNA-30e (miR-30e) and microRNA-223 (miR-223) as diagnostic biomarkers for early diagnosis of hepatocellular carcinoma (HCC) associated with hepatitis C virus (HCV) infection. Methods. The study included three groups, the first group included thirty patients with HCC associated with HCV, the second group included thirty patients with cirrhosis with HCV and the third group included thirty healthy control subjects. Blood samples were obtained for determination of serum expression of miR-30e and miR-223 by real time polymerase chain reaction. Results. There was significant decrease of miR-30e of expression in patients with HCC (0.16 ± 0.1) compared to both patients with cirrhosis (0.4 ± 0.2, P<0.01) and healthy control subjects (1.2 ± 0.4, P<0.001). There was also significant reduction of miR-223 expression levels in patients with HCC (0.2 ± 0.1) compared to patients with cirrhosis (0.5 ± 0.2, P<0.01) and healthy control subjects (1.0 ± 0.1, P<0.001). There was also significant decrease of miR-30e expression in late stage versus early stage of HCC (0.1 ± 0.0 vs. 0.22 ± 0.1, P<0.001), while there was no significant difference of alpha-fetoprotein (AFP) between patients with late and early HCC. Also, values of miR-223 had significantly reduced levels in late HCC compared to its expression values in early HCC (0.1± 0.0 vs. 0.23 ± 0.2, P<001). Conclusion. There was significant reduction of expression of miR-30e and miR-223 in serum of HCC patients compared to either patients with cirrhosis or healthy subjects. These results show that the combined use of both biomarkers had better sensitivity in the diagnosis of HCC compared to AFP.

scholarly journals Effects of Different Disinfection Methods on Microleakage of Giomer Restorations

2019 ◽  
Vol 13 (04) ◽  
pp. 569-573 ◽  
Author(s):  
Abidin Talha Mutluay ◽  
Merve Mutluay
Keyword(s):  
In Vitro Study ◽  
Study Groups ◽  
Class V ◽  
Disinfection Methods ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Method Group

Abstract Objectives The purpose of this in vitro study was to investigate the effects of different disinfection methods on microleakage of Class V Giomer restorations. Materials and Methods Class V cavity preparation was performed on 40 sound permanent central incisors. Class V cavities (3 x 2 x 2 mm) were prepared on the buccal surfaces of the selected teeth. The teeth were randomly divided into four groups, each to be disinfected with a different method: Group 1—Disinfection with 2% chlorhexidine, Group 2—Disinfection with 6% sodium hypochlorite, Group 3—Disinfection with erbium, chromium: yttrium-scandium-gallium-garnet laser, Group 4—Control (distilled water). BeautiBond adhesive and Beautifil II Giomer restoration materials were applied to all surfaces of the cavities after the disinfection of all groups. The teeth were thermocycled 5,000 times (5–55°C), and then were immersed in 0.5% methylene blue for 24 hours. After rinsing, the teeth were longitudinally sectioned and dye penetration was assessed under a stereomicroscope (40x) to evaluate microleakage. Statistical Analysis The results were statistically evaluated by using the Kruskal–Wallis and Mann–Whitney U tests. Results Statistically, no significant difference was found between all four groups (p > 0.05). There was no statistically significant difference in microleakage scores of study groups (p > 0.05). Also, the microleakage scores of the gingival margins were significantly higher than the incisal margins (p < 0.0001). Conclusions Application of the different cavity disinfectants has no effect on the microleakage of Class V Giomer restorations.

scholarly journals Epidemiological Characteristics of Advanced Hepatocellular Carcinoma in the Northern Region of Vietnam

2019 ◽  
Vol 26 (1) ◽  
pp. 107327481986279 ◽  
Author(s):  
Van Quang Le ◽  
Van Hieu Nguyen ◽  
Van Hung Nguyen ◽  
Thanh Long Nguyen ◽  
Staci L. Sudenga ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hcv Infection ◽  
High Rate ◽  
Advanced Hepatocellular Carcinoma ◽  
Female Ratio ◽  
Advanced Hcc ◽  
Significant Difference ◽  
Epidemiological Characteristics

Epidemiological characteristics of hepatocellular carcinoma (HCC) in Southern Vietnam has been well reported as in Globocan 2018 while data from the North has still not been fully presented. Therefore, we conducted this retrospective descriptive study on 198 advanced HCC patients treated at 3 major hospitals in Northern Vietnam to describe demographic features, HCC risk factors, and correlation among them in patients with advanced HCC. This information will lead to prevention efforts and provide information for allocating funds for treatment. The median age at diagnosis was 57 years (range: 19-86) and the male/female ratio was 8.9/1. The proportions of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection were 81.3% and 5.6%, respectively. Hepatitis C virus infection rate was significantly higher in patients <50 years old (12.5% vs 3.3%, P = .016). There was no significant difference in age or viral hepatitis infection status by gender. Only 7.6% of patients diagnosed with advanced HCC were asymptomatic. In conclusion, with the high rate of HBV infection among patients with advanced HCC, it is necessary for increasing prevention efforts in HBV screening. Furthermore, HCV infection should be noticed in patients with advanced HCC younger than 50 years old.

scholarly journals Sensitivity and Specificity of Belin Ambrosio Enhanced Ectasia Display in Early Diagnosis of Keratoconus

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Shahram Bamdad ◽  
Mohammad Reza Sedaghat ◽  
Masoud Yasemi ◽  
Aliraza Vahedi
Keyword(s):  
Early Diagnosis ◽  
Sensitivity And Specificity ◽  
Study Groups ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Normal Thickness

Purpose. Early diagnosis of keratoconus disease (KCN) is the first priority in the preoperative evaluations of refractive surgery (RS).The aim of this study was to investigate the correlation between findings of Belin Ambrosio enhanced ectasia display (BAD) software and conventional corneal imaging (Orbscan and topography) in the early diagnosis of KCN. Methods. For conducting this cross-sectional study, a total of 1000 eyes were selected from 500 patients that underwent the myopic photorefractive keratectomy surgery and were compared in four study groups during the years 2017–2018. In group 1, all topography, Orbscan, and BAD criteria were normal (65.8%).In contrast, in Group 2, at least one of the topography or Orbscan criteria as well as at least one BAD criterion (12.6%) were abnormal. In Group 3, the eyes had normal Orbscan and topography criteria with at least one abnormal BAD criterion (18.5%). Also, in Group 4, the patients had at least one abnormal Orbscan or topography criterion, but all BAD criteria (3.1%) were normal. Thickness of the thinnest point (TP) of cornea was compared in Pentacam and topography. Data analysis was done by SPSS software (version 21). Results. BAD criteria were normal in 78.5% of all eyes with normal topography and Orbscan criteria (specificity). BAD criteria were also abnormal in 80.2% of eyes (sensitivity). There was also no significant difference between TP in Orbscan and Pentacam. Conclusions. BAD criteria had a relatively acceptable sensitivity and specificity, compared with conventional Orbscan and topography criteria. Thus, BAD criteria can be more effective in the early diagnosis of KCN.

Determinants of the future burden of hepatocellular carcinoma after eradication of hepatitis C virus among cirrhotic patients

2017 ◽  
Vol 66 (1) ◽  
pp. S702-S703
Author(s):  
P. Udompap ◽  
A. Mannalithara ◽  
A.J. Kwong ◽  
D. Kim ◽  
R. Dhanasekaran ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
The Future ◽  
Future Burden ◽  
Cirrhotic Patients
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