scholarly journals Arabidopsis thaliana Seedlings Influence Bacillus subtilis Spore Formation

2019 ◽  
Vol 32 (9) ◽  
pp. 1188-1195 ◽  
Author(s):  
Vincent Charron-Lamoureux ◽  
Pascale B. Beauregard
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacillus Subtilis ◽  
Systemic Resistance ◽  
Soil System ◽  
Beneficial Effects ◽  
Bacillus Subtilis Spore ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus subtilis is a Gram-positive plant-growth-promoting rhizobacterium exerting many beneficial effects on plant health. Because they secrete antimicrobial compounds and elicit induced systemic resistance, B. subtilis and phylogenetically related species are of particular interest as antifungals in organic agriculture. These bacteria are also known for their capacity to differentiate phenotypically into endospores able to withstand many environmental stresses. However, although B. subtilis is often inoculated on plants as spores, dynamics of germination and sporulation on roots remain unexplored. Using a hydroponic culture system and a soil system for Arabidopsis thaliana, we observed that B. subtilis spores germinate rapidly on contact with plants. However, the vegetative cells are abundant on roots for only a few days before reversing back to spores. We observed that the germinant receptor GerK and sporulation kinases KinA and KinB identified in vitro control sporulation dynamics on plants. Surprisingly, when plants are inoculated with B. subtilis, free-living cells sporulate more rapidly than plant-associated cells. However, direct contact between plant and bacteria is required for the induction of sporulation in the surrounding B. subtilis. This study has fundamental implications for our understanding of interactions between Bacillus spp. and plants, and particularly for a more efficient usage of B. subtilis as a biofertilizer or biofungicide.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Biocontrol of Large Patch Disease in Zoysiagrass (Zoysia japonica) by Bacillus subtilis SA-15: Identification of Active Compounds and Synergism with a Fungicide

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 34
Author(s):  
Young-Sun Kim ◽  
Kyo-Suk Lee ◽  
Hong-Gi Kim ◽  
Geung-Joo Lee
Keyword(s):  
Bacillus Subtilis ◽  
Mycelial Growth ◽  
Zoysia Japonica ◽  
Large Patch ◽  
Control Efficacy ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Patch Disease ◽  
Farming Soil

Bacillus subtilis SA-15 is a plant growth-promoting bacterium isolated from non-farming soil. We aimed to identify lipopeptides produced by B. subtilis SA-15 and evaluate the control efficacy of B. subtilis SA-15 against large patch disease caused by Rhizoctonia solani AG 2-2 (IV) in zoysiagrass (Zoysia japonica). Bacillus subtilis SA-15 inhibited mycelial growth of R. solani AG 2-2 (IV) in vitro and produced fengycin A and dehydroxyfengycin A, which are antifungal compounds. Fengycin A and deghydroxyfengycin A inhibited R. solani mycelial growth by 30.4 and 63.2%, respectively. We formulated B. subtilis SA-15 into a wettable powder and determined its control efficiency against large patch in a field trial. The control efficacy was 51.2–92.0%. Moreover, when B. subtilis SA-15 powder was applied together with half the regular dose of the fungicide pecycuron, the control efficacy was 88.5–100.0%. These results indicate that B. subtilis SA-15 can be used to control soil-borne diseases, including large patch caused by R. solani, because of lipopeptide production. The use of this bacterium can also reduce the amount of fungicide needed, providing an eco-friendly management option for turfgrass.

scholarly journals Bacillus subtilisEarly Colonization ofArabidopsis thalianaRoots Involves Multiple Chemotaxis Receptors

mBio ◽  
2016 ◽  
Vol 7 (6) ◽  
Author(s):  
Rosalie Allard-Massicotte ◽  
Laurence Tessier ◽  
Frédéric Lécuyer ◽  
Venkatachalam Lakshmanan ◽  
Jean-François Lucier ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Root Exudates ◽  
Plant Protection ◽  
Orphan Receptor ◽  
Natural Environments ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
First Contact

ABSTRACTColonization of plant roots byBacillus subtilisis mutually beneficial to plants and bacteria. Plants can secrete up to 30% of their fixed carbon via root exudates, thereby feeding the bacteria, and in return the associatedB. subtilisbacteria provide the plant with many growth-promoting traits. Formation of a biofilm on the root by matrix-producingB. subtilisis a well-established requirement for long-term colonization. However, we observed that cells start forming a biofilm only several hours after motile cells first settle on the plant. We also found that intact chemotaxis machinery is required for early root colonization byB. subtilisand for plant protection.Arabidopsis thalianaroot exudates attractB. subtilis in vitro, an activity mediated by the two characterized chemoreceptors, McpB and McpC, as well as by the orphan receptor TlpC. Nonetheless, bacteria lacking these chemoreceptors are still able to colonize the root, suggesting that other chemoreceptors might also play a role in this process. These observations suggest thatA. thalianaactively recruitsB. subtilisthrough root-secreted molecules, and our results stress the important roles ofB. subtilischemoreceptors for efficient colonization of plants in natural environments. These results demonstrate a remarkable strategy adapted by beneficial rhizobacteria to utilize carbon-rich root exudates, which may facilitate rhizobacterial colonization and a mutualistic association with the host.IMPORTANCEBacillus subtilisis a plant growth-promoting rhizobacterium that establishes robust interactions with roots. Many studies have now demonstrated that biofilm formation is required for long-term colonization. However, we observed that motileB. subtilismediates the first contact with the roots. These cells differentiate into biofilm-producing cells only several hours after the bacteria first contact the root. Our study reveals that intact chemotaxis machinery is required for the bacteria to reach the root. Many, if not all, of theB. subtilis10 chemoreceptors are involved in the interaction with the plant. These observations stress the importance of root-bacterium interactions in theB. subtilislifestyle.

scholarly journals In Vitro Evaluation and Genome Mining of Bacillus subtilis Strain RS10 Reveals Its Biocontrol and Plant Growth-Promoting Potential

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1273
Author(s):  
Sajid Iqbal ◽  
Nimat Ullah ◽  
Hussnain Ahmed Janjua
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Genome Analysis ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Subtilis Strain ◽  
Antifungal Metabolites ◽  
Plant Growth Promoting ◽  
Growth Promoting

Recently, crop management has involved excessive use of chemical fertilizers and pesticides, compromising public health and environmental integrity. Rhizobacteria, which can enhance plant growth and protect plants from phytopathogen, are eco-friendly and have been attracting increasing attention. In the current study, Bacillus subtilis RS10 isolated from the rhizosphere region of Cynodon dactylon, inhibited the growth of indicator strains and exhibited in vitro plant growth-promoting traits. A whole-genome analysis identified numerous biosynthetic gene clusters encoding antibacterial and antifungal metabolites including bacillibactin, bogorol A, fengycin, bacteriocin, type III polyketides (PKs), and bacilysin. The plant growth-promoting conferring genes involved in nitrogen metabolism, phosphate solubilization, hydrogen sulfide, phytohormones, siderophore biosynthesis, chemotaxis and motility, plant root colonization, lytic enzymes, and biofilm formation were determined. Furthermore, genes associated with abiotic stresses such as high salinity and osmotic stress were identified. A comparative genome analysis indicated open pan-genome and the strain was identified as a novel sequence type (ST-176). In addition, several horizontal gene transfer events were found which putatively play a vital role in the evolution and new functionalities of a strain. In conclusion, the current study demonstrates the potential of RS10 antagonism against important pathogens and plant growth promotion, highlighting its application in sustainable agriculture.

scholarly journals Systemic Resistance Induced by Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungi in Arabidopsis thaliana

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86882 ◽  
Author(s):  
Hushna Ara Naznin ◽  
Daigo Kiyohara ◽  
Minako Kimura ◽  
Mitsuo Miyazawa ◽  
Masafumi Shimizu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Volatile Organic Compounds ◽  
Plant Growth ◽  
Organic Compounds ◽  
Systemic Resistance ◽  
Volatile Organic ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Protection Against Pathogen and Salt Stress by Four Plant Growth-Promoting Rhizobacteria Isolated from Pinus sp. on Arabidopsis thaliana

2008 ◽  
Vol 98 (6) ◽  
pp. 666-672 ◽  
Author(s):  
J. Barriuso ◽  
B. Ramos Solano ◽  
F. J. Gutiérrez Mañero
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Induce Systemic Resistance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Dependent Pathway

The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance to salt stress (NaCl 60 mM). Bacillus sp. strain L81 and Arthrobacter oxidans strain BB1 performed best with a decrease in the disease index of 61.2 and 52.3%, respectively, and a reduction in the mortality due to salt stress of 72.4 and 57.8%, respectively. Additionally, significant differences were found in growth and photosynthesis, again, L81 and BB1 performed best either in normal or under stress conditions. In order to elucidate the pathway elicited by these two strains to induce systemic resistance, experiments with the transgenic line of Arabidopsis thaliana NahG (defective in salicylic acid [SA]) and with the jar1 mutant (defective in jasmonic acid) were carried out. Results showed that the SA-dependent pathway was involved in the defense response induced by strains L81 and BB1. Results from quantitative reverse transcription-polymerase chain reaction analysis of the PR1 gene, related to the SA-dependent pathway and the PDF1.2 gene related to the SA-independent pathway, showed an increased expression of PR1 in BB1-treated plants, confirming involvement of the SA-dependent pathway in the defensive response.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

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