The rsmA-like Gene rsmAXcc of Xanthomonas campestris pv. campestris Is Involved in the Control of Various Cellular Processes, Including Pathogenesis

RsmA is an RNA-binding protein functioning as a global post-transcriptional regulator of various cellular processes in bacteria and has been demonstrated to be an important virulence regulator in many animal bacterial pathogens. However, its function in other phytopathogenic bacteria is unclear, except for the Erwinia carotovora RsmA, which acts as a negative virulence regulator. In this work, we investigated the function of the rsmA-like gene, named rsmAXcc, of the phytopathogen Xanthomonas campestris pv. campestris. Deletion of rsmAXcc resulted in complete loss of virulence on the host plant Chinese radish, hypersensitive response on the nonhost plant pepper ECW-10R, and motility on the surface of an agar plate. The rsmAXcc mutant displayed a significant reduction in the production of extracellular amylase, endoglucanase, and polysaccharide, but a significant increase in intracellular glycogen accumulation and an enhanced bacterial aggregation and cell adhesion. Microarray hybridization and semiquantitative reverse-transcription polymerase chain reaction analysis showed that deletion of rsmAXcc led to significantly reduced expression of genes encoding the type III secretion system (T3SS), T3SS-effectors, and the bacterial aggregate dispersing enzyme endo-β-1,4-mannanase. These results suggest that rsmAXcc is involved in the control of various cellular processes, including pathogenesis of X. campestris pv. campestris.

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hpaR, a Putative marR Family Transcriptional Regulator, Is Positively Controlled by HrpG and HrpX and Involved in the Pathogenesis, Hypersensitive Response, and Extracellular Protease Production of Xanthomonas campestris Pathovar campestris

Journal of Bacteriology ◽

10.1128/jb.01331-06 ◽

2006 ◽

Vol 189 (5) ◽

pp. 2055-2062 ◽

Cited By ~ 49

Author(s):

Ke Wei ◽

Dong-Jie Tang ◽

Yong-Qiang He ◽

Jia-Xun Feng ◽

Bo-Le Jiang ◽

...

Keyword(s):

Hypersensitive Response ◽

Xanthomonas Campestris ◽

Transcriptional Regulator ◽

Transcriptional Regulators ◽

Extracellular Protease ◽

Protease Production ◽

Phytopathogenic Bacterium ◽

Cellular Processes ◽

Glucuronidase Activity ◽

Nonhost Plant

ABSTRACT The MarR family of transcriptional regulators of bacteria are involved in the regulation of many cellular processes, including pathogenesis. In this work, we have demonstrated genetically that hpaR (hpa, hrp associated), which encodes a putative MarR family regulator, is involved in the hypersensitive response (HR), pathogenicity, and extracellular protease production of the phytopathogenic bacterium Xanthomonas campestris pathovar campestris. A mutation in hpaR resulted in complete loss of virulence in the host plant cabbage, a delayed and weakened HR in the nonhost plant pepper ECW-10R, and an increase in extracellular protease production. Detection of the β-glucuronidase activity of a plasmid-driven hpaR promoter-gusA reporter revealed that the expression of hpaR is positively controlled by HrpG and HrpX and is suppressed in rich medium while being strongly induced in minimal and hrp-inducing media and inside the host. These findings indicate that hpaR belongs to the hrpG and hrpX regulon and that HrpX regulates the extracellular protease production via hpaR in X. campestris pv. campestris.

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Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant Brassica napus Cultivars Infected with Xanthomonas campestris pv. campestris

Microorganisms ◽

10.3390/microorganisms9020253 ◽

2021 ◽

Vol 9 (2) ◽

pp. 253

Author(s):

Md Tabibul Islam ◽

Bok-Rye Lee ◽

Van Hien La ◽

Dong-Won Bae ◽

Woo-Jin Jung ◽

...

Keyword(s):

Innate Immunity ◽

Brassica Napus ◽

Quantitative Proteomics ◽

Xanthomonas Campestris ◽

Rna Binding ◽

Protein Profile ◽

Mitochondrial Outer Membrane ◽

Ps Ii ◽

Related Proteins ◽

Xanthomonas Campestris Pv Campestris

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is the main disease of cruciferous vegetables. To characterize the resistance mechanism in the Brassica napus–Xcc pathosystem, Xcc-responsive proteins in susceptible (cv. Mosa) and resistant (cv. Capitol) cultivars were investigated using gel-free quantitative proteomics and analysis of gene expression. This allowed us to identify 158 and 163 differentially expressed proteins following Xcc infection in cv. Mosa and cv. Capitol, respectively, and to classify them into five major categories including antioxidative systems, proteolysis, photosynthesis, redox, and innate immunity. All proteins involved in protein degradation such as the protease complex, proteasome subunits, and ATP-dependent Clp protease proteolytic subunits, were upregulated only in cv. Mosa, in which higher hydrogen peroxide accumulation concurred with upregulated superoxide dismutase. In cv. Capitol, photosystem II (PS II)-related proteins were downregulated (excepting PS II 22 kDa), whereas the PS I proteins, ATP synthase, and ferredoxin-NADP+ reductase, were upregulated. For redox-related proteins, upregulation of thioredoxin, 2-cys peroxiredoxin, and glutathione S-transferase occurred in cv. Capitol, consistent with higher NADH-, ascorbate-, and glutathione-based reducing potential, whereas the proteins involved in the C2 oxidative cycle and glycolysis were highly activated in cv. Mosa. Most innate immunity-related proteins, including zinc finger domain (ZFD)-containing protein, glycine-rich RNA-binding protein (GRP) and mitochondrial outer membrane porin, were highly enhanced in cv. Capitol, concomitant with enhanced expression of ZFD and GRP genes. Distinguishable differences in the protein profile between the two cultivars deserves higher importance for breeding programs and understanding of disease resistance in the B. napus–Xcc pathosystem.

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Chemotaxis of some phytopathogenic bacteria to fungal propagules in vitro and in soil

Canadian Journal of Microbiology ◽

10.1139/m88-037 ◽

1988 ◽

Vol 34 (2) ◽

pp. 196-199 ◽

Cited By ~ 7

Author(s):

W. C. Lim ◽

J. L. Lockwood

Keyword(s):

Pseudomonas Syringae ◽

Xanthomonas Campestris ◽

Pathogenic Bacteria ◽

Erwinia Carotovora ◽

Bipolaris Sorokiniana ◽

Macrophomina Phaseolina ◽

Plant Pathogenic Bacteria ◽

Fungal Propagules ◽

Xanthomonas Campestris Pv Campestris

The motile plant pathogenic bacteria Erwinia carotovora pv. carotovora, Pseudomonas syringae pv. phaseolicola, and Xanthomonas campestris pv. campestris were strongly attracted to conidia of Bipolaris sorokiniana, B. victoriae, and to sclerotia of Macrophomina phaseolina and their exudates in vitro and in soil, but not to phosphate buffer or buffer–soil mixtures. Bacteria accumulated radioactivity within 1 h after being placed in exudates from 14C-labelled conidia of B. sorokiniana. After 5 h, radioactivity of the 14C-labelled exudate was reduced to 29–54% of that in the original medium. Exudates from fungal propagules may act as attractants and substrates for motile plant pathogenic bacteria in soil.

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Xanthomonas campestris pv. campestris (black rot).

10.1079/cpc.56919.20210100335 ◽

2021 ◽

Author(s):

Carmen Nieves Mortensen

Keyword(s):

New Zealand ◽

Xanthomonas Campestris ◽

Central And Eastern Europe ◽

Erwinia Carotovora ◽

Soft Rot ◽

Crop Damage ◽

Northern Europe ◽

Black Rot ◽

Opportunistic Pathogens ◽

Xanthomonas Campestris Pv Campestris

Abstract Black rot is considered the most important worldwide disease of crucifers. The disease is known to exist in the cool coastal climates of northern Europe and North America but was seldom a problem there until the 1990s. Its potential for crop damage is also considered low in New Zealand, and parts of Australia. In many regions of Central and Eastern Europe, Central Asia (Kazakhstan), China, tropical and subtropical regions of Asia, Africa and South America where brassicas are common and cultivated without crop rotation, black rot is always present. Seed production in those regions is commonly associated with high levels of seedborne Xcc (Williams, 1980). Even minor, visually undetectable development of black rot may considerably increase damage to plants by soft rot caused by Erwinia carotovora, Pseudomonas spp. and other opportunistic pathogens (Djalilov et al., 1989).

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Isolation and Identification of Xanthomonas Campestris Pv. Campestris Causing Black Rot on Cauliflower From Plains of Kerala

International Journal of Scientific Research ◽

10.15373/22778179/feb2014/2 ◽

2012 ◽

Vol 3 (2) ◽

pp. 4-6

Author(s):

S. Lakshmi Prasanna ◽

◽

Dr. S. Ravi Dr. S. Ravi

Keyword(s):

Xanthomonas Campestris ◽

Black Rot ◽

Isolation And Identification ◽

Xanthomonas Campestris Pv Campestris

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BIOCHEMICAL IDENTIFICATION OF PATOVARS OF THE PATHOGEN OF VASCULAR BACTERIOSIS OF THE CRUCIFEROUS XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS

SCIENTIFIC LIFE ◽

10.35679/1991-9476-2020-15-1-82-88 ◽

2020 ◽

Vol 15 (1) ◽

pp. 82-88

Author(s):

Mikhail Kuznetsov ◽

◽

Anatoly Scherbakov ◽

Elena Gorelnikova ◽

Nadezhda Chervyakova ◽

...

Keyword(s):

Xanthomonas Campestris ◽

Biochemical Identification ◽

Xanthomonas Campestris Pv Campestris

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C9orf72-associated arginine-rich dipeptide repeats induce RNA-dependent nuclear accumulation of Staufen in neurons

Human Molecular Genetics ◽

10.1093/hmg/ddab089 ◽

2021 ◽

Author(s):

Eun Seon Kim ◽

Chang Geon Chung ◽

Jeong Hyang Park ◽

Byung Su Ko ◽

Sung Soon Park ◽

...

Keyword(s):

Retinal Degeneration ◽

Rna Binding ◽

Rna Binding Proteins ◽

Nuclear Accumulation ◽

Heterozygous Mutation ◽

Pathological Feature ◽

Dependent Manner ◽

Cellular Processes ◽

Drosophila Model ◽

Post Transcriptional Regulation

Abstract RNA-binding proteins (RBPs) play essential roles in diverse cellular processes through post-transcriptional regulation of RNAs. The subcellular localization of RBPs is thus under tight control, the breakdown of which is associated with aberrant cytoplasmic accumulation of nuclear RBPs such as TDP-43 and FUS, well-known pathological markers for amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD). Here, we report in Drosophila model for ALS/FTD that nuclear accumulation of a cytoplasmic RBP, Staufen, may be a new pathological feature. We found that in Drosophila C4da neurons expressing PR36, one of the arginine-rich dipeptide repeat proteins (DPRs), Staufen accumulated in the nucleus in Importin- and RNA-dependent manner. Notably, expressing Staufen with exogenous NLS—but not with mutated endogenous NLS—potentiated PR-induced dendritic defect, suggesting that nuclear-accumulated Staufen can enhance PR toxicity. PR36 expression increased Fibrillarin staining in the nucleolus, which was enhanced by heterozygous mutation of stau (stau+/−), a gene that codes Staufen. Furthermore, knockdown of fib, which codes Fibrillarin, exacerbated retinal degeneration mediated by PR toxicity, suggesting that increased amount of Fibrillarin by stau+/− is protective. Stau+/− also reduced the amount of PR-induced nuclear-accumulated Staufen and mitigated retinal degeneration and rescued viability of flies expressing PR36. Taken together, our data show that nuclear accumulation of Staufen in neurons may be an important pathological feature contributing to the pathogenesis of ALS/FTD.

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