scholarly journals Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant Brassica napus Cultivars Infected with Xanthomonas campestris pv. campestris

2021 ◽  
Vol 9 (2) ◽  
pp. 253
Author(s):  
Md Tabibul Islam ◽  
Bok-Rye Lee ◽  
Van Hien La ◽  
Dong-Won Bae ◽  
Woo-Jin Jung ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Brassica Napus ◽  
Quantitative Proteomics ◽  
Xanthomonas Campestris ◽  
Rna Binding ◽  
Protein Profile ◽  
Mitochondrial Outer Membrane ◽  
Ps Ii ◽  
Related Proteins ◽  
Xanthomonas Campestris Pv Campestris

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is the main disease of cruciferous vegetables. To characterize the resistance mechanism in the Brassica napus–Xcc pathosystem, Xcc-responsive proteins in susceptible (cv. Mosa) and resistant (cv. Capitol) cultivars were investigated using gel-free quantitative proteomics and analysis of gene expression. This allowed us to identify 158 and 163 differentially expressed proteins following Xcc infection in cv. Mosa and cv. Capitol, respectively, and to classify them into five major categories including antioxidative systems, proteolysis, photosynthesis, redox, and innate immunity. All proteins involved in protein degradation such as the protease complex, proteasome subunits, and ATP-dependent Clp protease proteolytic subunits, were upregulated only in cv. Mosa, in which higher hydrogen peroxide accumulation concurred with upregulated superoxide dismutase. In cv. Capitol, photosystem II (PS II)-related proteins were downregulated (excepting PS II 22 kDa), whereas the PS I proteins, ATP synthase, and ferredoxin-NADP+ reductase, were upregulated. For redox-related proteins, upregulation of thioredoxin, 2-cys peroxiredoxin, and glutathione S-transferase occurred in cv. Capitol, consistent with higher NADH-, ascorbate-, and glutathione-based reducing potential, whereas the proteins involved in the C2 oxidative cycle and glycolysis were highly activated in cv. Mosa. Most innate immunity-related proteins, including zinc finger domain (ZFD)-containing protein, glycine-rich RNA-binding protein (GRP) and mitochondrial outer membrane porin, were highly enhanced in cv. Capitol, concomitant with enhanced expression of ZFD and GRP genes. Distinguishable differences in the protein profile between the two cultivars deserves higher importance for breeding programs and understanding of disease resistance in the B. napus–Xcc pathosystem.

scholarly journals Quantitative Proteomics Reveals the Defense Response of Wheat against Puccinia striiformis f. sp. tritici

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yuheng Yang ◽  
Yang Yu ◽  
Chaowei Bi ◽  
Zhensheng Kang
Keyword(s):  
Quantitative Proteomics ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Puccinia Striiformis ◽  
Post Inoculation ◽  
Control Groups ◽  
Stimulus Response ◽  
Wheat Gene ◽  
Related Proteins ◽  
And Control

Abstract Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is considered one of the most aggressive diseases to wheat production. In this study, we used an iTRAQ-based approach for the quantitative proteomic comparison of the incompatible Pst race CYR23 in infected and non-infected leaves of the wheat cultivar Suwon11. A total of 3,475 unique proteins were identified from three key stages of interaction (12, 24, and 48 h post-inoculation) and control groups. Quantitative analysis showed that 530 proteins were differentially accumulated by Pst infection (fold changes >1.5, p < 0.05). Among these proteins, 10.54% was classified as involved in the immune system process and stimulus response. Intriguingly, bioinformatics analysis revealed that a set of reactive oxygen species metabolism-related proteins, peptidyl–prolyl cis–trans isomerases (PPIases), RNA-binding proteins (RBPs), and chaperonins was involved in the response to Pst infection. Our results were the first to show that PPIases, RBPs, and chaperonins participated in the regulation of the immune response in wheat and even in plants. This study aimed to provide novel routes to reveal wheat gene functionality and better understand the early events in wheat–Pst incompatible interactions.

scholarly journals Identification of Sources of Resistance to Xanthomonas campestris pv. campestris in Brassica napus Crops

Plant Disease ◽  
2011 ◽  
Vol 95 (3) ◽  
pp. 292-297 ◽  
Author(s):  
M. Lema ◽  
P. Soengas ◽  
P. Velasco ◽  
M. Francisco ◽  
M. E. Cartea
Keyword(s):  
Brassica Napus ◽  
Xanthomonas Campestris ◽  
Specific Resistance ◽  
Sources Of Resistance ◽  
Nonspecific Resistance ◽  
Breeding Programs ◽  
Race 1 ◽  
Improved Cultivars ◽  
Race 4 ◽  
Xanthomonas Campestris Pv Campestris

Black rot, caused by Xanthomonas campestris pv. campestris, is one of the most important diseases affecting Brassica crops worldwide. Nine races have been differentiated in X. campestris pv. campestris, with races 1 and 4 being the most virulent and widespread. The objective of this work was to identify sources of resistance to races 1 and 4 of X. campestris pv. campestris in different Brassica napus crops, mainly in the underexplored pabularia group. Seventy-six accessions belonging to four B. napus groups were screened for resistance to two X. campestris pv. campestris races (1 and 4). The strain of race 1 used in this study was more virulent on the tested materials than the strain of race 4. No race-specific resistance was found to race 1. Most cultivars were susceptible except Russian kale, from the pabularia group, which showed some resistant plants and some other accessions with some partially resistant plants. High levels of race-specific resistance to race 4 were found in the pabularia group, and great variability within accessions was identified. Three improved cultivars (Ragged Jack kale, Friese Gele, and Valle del Oro) and four landraces (Russian kale, MBG-BRS0037, MBG-BRS0041, and MBG-BRS0131) showed plants with some degree of resistance to both races, which may indicate that race-nonspecific resistance is involved. These accessions could be directly used in breeding programs, either as improved cultivars or as donors of race-specific resistance to other Brassica cultivars.

scholarly journals Terapia fotodinâmica no controle de Xanthomonas campestris pv. campestris in vitro e no tratamento de sementes de canola naturalmente contaminadas

2020 ◽  
Vol 46 (4) ◽  
pp. 327-332
Author(s):  
Nayara Lima Baute Zancan ◽  
Nilvanira Donizete Tebaldi
Keyword(s):  
Brassica Napus ◽  
Xanthomonas Campestris ◽  
Brassica Napus L ◽  
Xanthomonas Campestris Pv Campestris

RESUMO A cultura da canola (Brassica napus L. var. oleifera) foi recentemente introduzida na região do Triângulo Mineiro e Alta Paranaíba, MG. A podridão negra causada pela bactéria Xanthomonas campestris pv. campestris (Xcc) é uma das principais doenças da cultura. A bactéria é disseminada pelas sementes e métodos alternativos de controle devem ser avaliados. Diante disso, o objetivo deste trabalho foi avaliar o uso da terapia fotodinâmica, com os corantes Azul de Metileno (AM) e Azul de Toluidina (AT), sob à irradiação, na inibição do crescimento de Xcc in vitro e no tratamento de sementes de canola naturalmente contaminadas com a bactéria. A suspensão bacteriana de Xcc foi tratada com os corantes AM, AT e associação deles (AM+AT) nas concentrações 25, 50 e 100 µmol L-1, irradiadas ou não, e cultivada em meio de cultura, em seguida foi avaliado o número de unidades formadoras de colônias. Sementes de três genótipos de canola foram tratadas com NaCl 0,45% (Testemunha), AM, AT e AM+AT, nas concentrações 100, 50 e 25 µmol L-1, respectivamente, irradiadas ou não. A porcentagem de germinação das sementes, índice de velocidade de emergência, porcentagem de emergência de plântulas e o controle da bactéria nas sementes foram avaliados. Os corantes AM, AT e AM+AT, nas concentrações 100, 50 e 25 µmol L-1 respectivamente, sob irradiação inibiram o crescimento de Xcc in vitro. A combinação dos corantes AM+AT a 25 µmol L-1 pode ser utilizada no tratamento das sementes de canola. O controle da bactéria Xcc em sementes de canola naturalmente contaminada não foi possível ser determinada com os diferentes corantes.

scholarly journals The rsmA-like Gene rsmAXcc of Xanthomonas campestris pv. campestris Is Involved in the Control of Various Cellular Processes, Including Pathogenesis

2008 ◽  
Vol 21 (4) ◽  
pp. 411-423 ◽  
Author(s):  
Nai-Xia Chao ◽  
Ke Wei ◽  
Qi Chen ◽  
Qing-Lin Meng ◽  
Dong-Jie Tang ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Rna Binding ◽  
Agar Plate ◽  
Erwinia Carotovora ◽  
Polymerase Chain Reaction Analysis ◽  
Glycogen Accumulation ◽  
Cellular Processes ◽  
Nonhost Plant ◽  
Virulence Regulator ◽  
Xanthomonas Campestris Pv Campestris

RsmA is an RNA-binding protein functioning as a global post-transcriptional regulator of various cellular processes in bacteria and has been demonstrated to be an important virulence regulator in many animal bacterial pathogens. However, its function in other phytopathogenic bacteria is unclear, except for the Erwinia carotovora RsmA, which acts as a negative virulence regulator. In this work, we investigated the function of the rsmA-like gene, named rsmAXcc, of the phytopathogen Xanthomonas campestris pv. campestris. Deletion of rsmAXcc resulted in complete loss of virulence on the host plant Chinese radish, hypersensitive response on the nonhost plant pepper ECW-10R, and motility on the surface of an agar plate. The rsmAXcc mutant displayed a significant reduction in the production of extracellular amylase, endoglucanase, and polysaccharide, but a significant increase in intracellular glycogen accumulation and an enhanced bacterial aggregation and cell adhesion. Microarray hybridization and semiquantitative reverse-transcription polymerase chain reaction analysis showed that deletion of rsmAXcc led to significantly reduced expression of genes encoding the type III secretion system (T3SS), T3SS-effectors, and the bacterial aggregate dispersing enzyme endo-β-1,4-mannanase. These results suggest that rsmAXcc is involved in the control of various cellular processes, including pathogenesis of X. campestris pv. campestris.

scholarly journals Quantitative proteomics indicate a strong correlation of mitotic phospho-/dephosphorylation with non-structured regions of substrates

2019 ◽  
Author(s):  
Hiroya Yamazaki ◽  
Hidetaka Kosako ◽  
Shige H. Yoshimura
Keyword(s):  
Protein Phosphorylation ◽  
Quantitative Proteomics ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Critical Role ◽  
Amino Acid Sequences ◽  
Mass Spectrometry Analysis ◽  
Order Structure ◽  
Ontology Term ◽  
Related Proteins

AbstractProtein phosphorylation plays a critical role in the regulation and progression of mitosis. More than 10,000 phosphorylated residues and the associated kinases have been identified to date via proteomic analyses. Although some of these phosphosites are associated with regulation of either protein-protein interactions or the catalytic activity of the substrate protein, the roles of most mitotic phosphosites remain unclear. In this study, we examined structural properties of mitotic phosphosites and neighboring residues to understand the role of heavy phosphorylation in non-structured domains. Quantitative mass spectrometry analysis of mitosis-arrested and non-arrested HeLa cells revealed >4,100 and >2,200 residues either significantly phosphorylated or dephosphorylated, respectively, at mitotic entry. The calculated disorder scores of amino acid sequences of neighboring individual phosphosites revealed that >70% of dephosphorylated phosphosites exist in disordered regions, whereas 50% of phosphorylated sites exist in non-structured domains. A clear inverse correlation was observed between probability of phosphorylation in non-structured domain and increment of phosphorylation in mitosis. These results indicate that at entry to mitosis, a significant number of phosphate groups are removed from non-structured domains and transferred to more-structured domains. Gene ontology term analysis revealed that mitosis-related proteins are heavily phosphorylated, whereas RNA-related proteins are both dephosphorylated and phosphorylated, suggesting that heavy phosphorylation/dephosphorylation in non-structured domains of RNA-binding proteins plays a role in dynamic rearrangement of RNA-containing organelles, as well as other intracellular environments.Significance StatementProgression of mitosis is tightly regulated by protein phosphorylation/dephosphorylation. Although proteomic studies have identified tens of thousands of phosphosites in mitotic cells, the roles of them remain to be answered. We approached this question from the viewpoint of the higher-order structure of phosphosites. Quantitative proteomics and bioinformatic analyses revealed that more than 70% of mitotic dephosphorylation events occurred in non-structured regions. Non-structured regions of cellular proteins are attracting considerable attention in terms of their involvement in dynamic rearrangements of intracellular membrane-less organelles and protein assembly/disassembly processes. Our results suggest the possibility that a vast amount of mitosis-associated dephosphorylation/phosphorylation at non-structured regions plays a role in regulating the dynamic assembly/disassembly of intracellular architectures and organelles such as chromosomes and nucleolus.

scholarly journals Genotypic Variation in Resistance Gene-Mediated Calcium Signaling and Hormonal Signaling Involved in Effector-Triggered Immunity or Disease Susceptibility in the Xanthomonas campestris pv. Campestris–Brassica napus Pathosystem

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 303
Author(s):  
Md. Al Mamun ◽  
Md. Tabibul Islam ◽  
Bok-Rye Lee ◽  
Van Hien La ◽  
Dong-Won Bae ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Protein Kinase ◽  
Calcium Signaling ◽  
Xanthomonas Campestris ◽  
Disease Susceptibility ◽  
R Gene ◽  
Leucine Rich Repeat ◽  
Calcium Sensing ◽  
Effector Triggered Immunity ◽  
Xanthomonas Campestris Pv Campestris

To characterize cultivar variation in resistance gene (R-gene)-mediated calcium signaling and hormonal regulation in effector-triggered immunity (ETI) and disease susceptibility, Xanthomonas campestris pv. campestris (Xcc) was inoculated in two Brassica napus cultivars (cvs. Capitol and Mosa). At 14 days post inoculation (DPI) with Xcc, there was a necrotic lesion in cv. Mosa along with the significant accumulation of H2O2 and malondialdehyde (MDA), whereas no visual symptom was observed in cv. Capitol. The cultivar variations in the R-gene expressions were found in response to Xcc. ZAR1 is a coiled-coil-nucleotide binding site-leucine-rich repeat (CC-NB-LRR)-type R-gene that is significantly induced in cv. Capitol, whereas toll/interleukin-1 receptor-nucleotide binding site-leucine-rich repeat (TIR-NB-LRR)-type R-gene, TAO1, is significantly upregulated in cv. Mosa Xcc-inoculated plants. The defense-related gene’s non-race-specific disease resistance 1 (NDR1) and mitogen-activated protein kinase 6 (MAPK6) were enhanced, whereas calcium-dependent protein kinase (CDPK5) and calcium-sensing protein 60g (CBP60g) were depressed in cv. Capitol Xcc inoculated plants, and opposite results were found in cv. Mosa. The calcium-sensing receptor (CAS), calmodulin (CaM), expression was induced in both the cultivars. However, the CAS induction rate was much higher in cv. Mosa than in cv. Capitol in response to Xcc. The phytohormone salicylic acid (SA) and jasmonic acid (JA) levels were significantly higher in cv. Capitol along with the enhanced SA receptors (NPR3 and NPR4) and JA synthesis and signaling-related gene expression (LOX2, PDF1.2), whereas the JA level was significantly lower in cv. Mosa Xcc inoculated plants. The SA synthesis and signaling-related genes (ICS1, NPR1) and SA were present at higher levels in cv. Mosa; additionally, the SA level present was much higher in the susceptible cultivar (cv. Mosa) than in the resistant cultivar (cv. Capitol) in response to Xcc. These results indicate that ZAR1 mediated the coordinated action of SA and JA synthesis and signaling to confirm ETI, whereas TAO1 enhanced the synthesis of SA through CAS and CBP60g to antagonize JA synthesis and signaling to cause disease susceptibility in the Brassica napus–Xcc pathosystem.

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