Resistances to Turnip Mosaic Potyvirus in Arabidopsis thaliana

The responses of a collection of Arabidopsis thaliana eco-types to mechanical inoculation with turnip mosaic poty-virus were assessed. The virus induced characteristic severe symptoms of infection in systemically infected plants. Resistance was found in four ecotypes: Bay-0, Di-0, Er-0, and Or-0. Enzyme-linked immunosorbent assay results of the resistant ecotypes suggested that ecotypes Di-0, Er-0, and Or-0 actually consist of mixed genotypes with resistances acting at different levels in the virus life cycle. Another form of resistance was found in ecotype Bay-0, for which several lines of evidence indicated an interference with viral cell-to-cell movement in the inoculated leaves.

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Detection of virus causes papaya ringspot virus - with the DAS-Elisa (Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay) method at different levels in North Sumatra

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/454/1/012182 ◽

2020 ◽

Vol 454 ◽

pp. 012182

Author(s):

L Hartati ◽

D Bakti ◽

A R Tantawi ◽

Lisnawita

Keyword(s):

Immunosorbent Assay ◽

Ringspot Virus ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Papaya Ringspot Virus ◽

Double Antibody ◽

North Sumatra ◽

Different Levels ◽

Das Elisa

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Transmission by Olpidium brassicae of Mirafiori lettuce virus and Lettuce big-vein virus, and Their Roles in Lettuce Big-Vein Etiology

Phytopathology ◽

10.1094/phyto.2002.92.3.288 ◽

2002 ◽

Vol 92 (3) ◽

pp. 288-293 ◽

Cited By ~ 72

Author(s):

Hervé Lot ◽

Robert N. Campbell ◽

Sylvie Souche ◽

Robert G. Milne ◽

Piero Roggero

Keyword(s):

Immunosorbent Assay ◽

Causal Agent ◽

Enzyme Linked Immunosorbent Assay ◽

Mechanical Transmission ◽

Mechanical Inoculation ◽

Resting Spores ◽

Specific Antisera ◽

Olpidium Brassicae ◽

Vein Disease

Big-vein disease occurs on lettuce worldwide in temperate conditions; the causal agent has been presumed to be Lettuce big-vein virus (LBVV), genus Varicosavirus, vectored by the soilborne fungus Olpidium brassicae. Recently, the role of LBVV in the etiology of big-vein disease has been questioned because a second soilborne virus, Mirafiori lettuce virus (MiLV), genus Ophiovirus, has been found frequently in big-vein-affected lettuce. LBVV and MiLV, detectable and distinguishable by enzyme-linked immunosorbent assay using specific antisera, were tested for their ability to be transmitted from lettuce to lettuce by mechanical inoculation of sap extracts, or by zoospores of O. brassicae, and to cause big-vein disease. Both viruses were mechanically transmissible from lettuce to herbaceous hosts and to lettuce, but very erratically. LBVV was transmitted by O. brassicae but lettuce infected with only this virus never showed symptoms. MiLV was transmitted in the same manner, and lettuce infected with this virus alone consistently developed big-vein symptoms regardless of the presence or absence of LBVV. With repeated mechanical transmission, isolates of both viruses appeared to lose the ability to be vectored, and MiLV appeared to lose the ability to cause big-vein symptoms. The recovery of MiLV (Mendocino isolate, from Cali-fornia) from stored O. brassicae resting spores puts the earliest directly demonstrable existence of MiLV at 1990.

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Determination of Total Fumonisins in Corn by Competitive Direct Enzyme-Linked Immunosorbent Assay: Collaborative Study

Journal of AOAC International ◽

10.1093/jaoac/85.2.404 ◽

2002 ◽

Vol 85 (2) ◽

pp. 404-410 ◽

Cited By ~ 26

Author(s):

Chuck B Bird ◽

Bruce Malone ◽

Larry G Rice ◽

P Frank Ross ◽

Robert Eppley ◽

...

Keyword(s):

Immunosorbent Assay ◽

Fusarium Species ◽

Collaborative Study ◽

The United States ◽

Enzyme Linked Immunosorbent Assay ◽

Test Portion ◽

Relative Standard ◽

Standard Deviations ◽

Different Levels

Abstract Fumonisins—mycotoxins produced by some Fusarium species—have been shown to be the causative agent of diseases in horses and other domesticated animals as well as possible carcinogens in humans. A collaborative study was conducted to evaluate the effectiveness of a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for the determination of total fumonisins (B1, B2, and B3) in corn. The test portion was extracted with methanol–water (7 + 3), filtered, diluted, and tested on the CD-ELISA. Naturally and artificially contaminated corn test portions were sent to 13 collaborators in the United States. Naturally contaminated field test portions were prepared at 3 different levels. Artificially contaminated test portions were spiked at 1.0, 3.0, and 5.0 mg/kg total fumonisins (B1, B2, and B3). Average recoveries of total fumonisins were 120, 100, and 90%, respectively. The relative standard deviations for repeatability ranged from 13.3 to 23.3% and the relative standard deviations for reproducibility ranged from 15.8 to 30.3% across all levels tested. HORRAT values, calculated for each individual sample, ranged from 1.24 to 1.94. This method demonstrated acceptable intra- and interlaboratory precision at the levels tested.

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Encapsidation of Potyviral RNA in Various Forms of Transgene Coat Protein Is Not Correlated with Resistance in Transgenic Plants

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.1997.10.8.1023 ◽

1997 ◽

Vol 10 (8) ◽

pp. 1023-1027 ◽

Cited By ~ 16

Author(s):

John Hammond ◽

Margaret M. Dienelt

Keyword(s):

Coat Protein ◽

Transgenic Plants ◽

Immunoelectron Microscopy ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Coated Plate ◽

Different Levels

Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from <1% to as much as 25% transgenic CP. Different levels of transcapsidation may reflect the extent of compatibility between transgene CP and the viral CP.

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Quantification of Soybean Seed Lectin in Soybean Tissues during the Life-Cycle of the Plant, by an Enzyme Linked Immunosorbent Assay (ELISA)

Proceedings of IUB Symposium No. 144, The Seventh International Lectin Meeting Bruxelles, Belgium, August 18–23, 1985 ◽

10.1515/9783112315958-082 ◽

1986 ◽

pp. 667-676

Keyword(s):

Life Cycle ◽

Immunosorbent Assay ◽

Soybean Seed ◽

Enzyme Linked Immunosorbent Assay

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Quantification of resin acids in CTMP (chemithermomechanical pulping) effluents using an enzyme-linked immunosorbent assay

Water Science & Technology ◽

10.2166/wst.1997.0491 ◽

1997 ◽

Vol 35 (2-3) ◽

pp. 93-99

Author(s):

K. Li ◽

A. Serreqi ◽

C. Breuil ◽

J. N. Saddler

Keyword(s):

Immunosorbent Assay ◽

Polyclonal Antibodies ◽

Resin Acids ◽

Enzyme Linked Immunosorbent Assay ◽

Inhibition Concentration ◽

Elisa Format ◽

Direct Elisa ◽

Chemithermomechanical Pulping ◽

Different Levels ◽

Elisa Data

An enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibodies was used to quantify resin acids in the effluents from a chemithermomechanical pulp (CTMP) mill. Using a direct ELISA format, with polyclonal antibodies immobilized on the plate, the assay had a 50% inhibition concentration (I50) of 49.7 ppb and a detection limit of 4.5 ppb. Good recoveries were obtained from DHA spiked buffer. CTMP effluents with different levels of resin acids were quantified and the ELISA data were shown to compare favourably with both total abietic type resin acids and total resin acids as determined by gas chromatography (GC).

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Resistance to Blueberry Shoestring Virus in Southern Highbush and Rabbiteye Cultivars

HortScience ◽

10.21273/hortsci.30.7.1459 ◽

1995 ◽

Vol 30 (7) ◽

pp. 1459-1460 ◽

Cited By ~ 3

Author(s):

Theresa Acquaah ◽

D.C. Ramsdell ◽

J.F. Hancock

Keyword(s):

United States ◽

Disease Resistance ◽

Immunosorbent Assay ◽

Southern United States ◽

Enzyme Linked Immunosorbent Assay ◽

Mechanical Inoculation ◽

Infection Rates ◽

Vaccinium Ashei ◽

Aphid Inoculation ◽

Southern Highbush

To determine if blueberry shoestring virus (BBSSV) is absent in the southern United States due to resistance of cultivars, we mechanically and rub-inoculated 1-year-old rooted microshoots of nine cultivars representing southern rabbiteye (Vaccinium ashei Reade), southern highbush (hybrids of V. corymbosum and V. darrowi Camp), and northern highbush (V. corymbosum L.). Leaves were sampled from plants, and enzyme-linked immunosorbent assay screened for the presence of virus over 15 months. Only a few individuals were infected after aphid inoculation, but many northern and southern cultivars became infected after mechanical inoculation. Northern highbush `Elliot' (50%) and `Blueray' (46.3%) had the highest infection rates, followed by rabbiteye `Climax' (36.3%) and the southern highbush `O'Neal' (12.5%). The lowest rates of infection were found in southern highbush `Georgiagem' (2.5%), `Misty' (2.5%), rabbiteye `Brightwell' (0.0%), and northern highbush `Bluecrop' (2.5%). Since many southern cultivars were infected by the disease, resistance likely has not excluded BBSSV from the southern United States.

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