scholarly journals Transgenic Expression of Pear PGIP in Tomato Limits Fungal Colonization

2000 ◽  
Vol 13 (9) ◽  
pp. 942-950 ◽  
Author(s):  
Ann L. T. Powell ◽  
Jan van Kan ◽  
Arjen ten Have ◽  
Jaap Visser ◽  
L. Carl Greve ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Heterologous Protein ◽  
Tomato Fruit ◽  
Fungal Growth ◽  
Fungal Colonization ◽  
Pear Fruit ◽  
Tomato Plants ◽  
Transgenic Expression ◽  
Transgenic Tomato Plants ◽  
Tissue Maceration

Transgenic tomato plants expressing the pear fruit polygalacturonase inhibitor protein (pPGIP) were used to demonstrate that this inhibitor of fungal pathogen endopolygalacturonases (endo-PGs) influences disease development. Transgenic expression of pPGIP resulted in abundant accumulation of the heterologous protein in all tissues and did not alter the expression of an endogenous tomato fruit PGIP (tPGIP). The pPGIP protein was detected, as expected, in the cell wall protein fraction in all transgenic tissues. Despite differential glycosylation in vegetative and fruit tissues, the expressed pPGIP was active in both tissues as an inhibitor of endo-PGs from Botrytis cinerea. The growth of B. cinerea on ripe tomato fruit expressing pPGIP was reduced, and tissue breakdown was diminished by as much as 15%, compared with nontransgenic fruit. In transgenic leaves, the expression of pPGIP reduced lesions of macerated tissue approximately 25%, a reduction of symptoms of fungal growth similar to that observed with a B. cinerea strain in which a single endo-PG gene, Bcpg1, had been deleted (A. ten Have, W. Mulder, J. Visser, and J. A. L. van Kan, Mol. Plant-Microbe Interact. 11:1009–1016, 1998). Heterologous expression of pPGIP has demonstrated that PGIP inhibition of fungal PGs slows the expansion of disease lesions and the associated tissue maceration.

scholarly journals Construction of CRISPR/Cas9 expression vectors habouring gRNA targeted on SLIAA9 gene of tomato

2020 ◽  
Vol 18 (1) ◽  
pp. 147-156
Author(s):  
Bui Manh Minh ◽  
Ha Hong Hanh ◽  
Le Thi Thu Hien ◽  
Huynh Thi Thu Hue
Keyword(s):  
Transgenic Plants ◽  
Genome Editing ◽  
Tomato Fruit ◽  
Expression System ◽  
Secondary Compounds ◽  
Expression Vectors ◽  
Tomato Plants ◽  
Normal Morphology ◽  
Transgenic Tomato Plants ◽  
Pcr Test

Tomato (Solanum lycopersicum) is a nutritious fruit containing many secondary compounds with health benefits. The formation of tomato fruit through fertilization is controlled by auxin through Aux/IAA9 and ARF8 proteins. The mutated SlIAA9 gene leads to the parthenocarpic development of fruit or seedless tomato fruit. Nowadays, the CRISPR/Cas9 genome editing system is becoming increasingly popular in modifying desired genes on plant objects. In this study, gRNAs which target on tomato SlIAA9 gene were designed and inserted into CRISPR/Cas9 vectors. In addition, two strains of A. tumefaciens harboring pRGEB31-IAA9G2 and pRGEB32-IAA9G2 vectors carrying CRISPR/Cas9 expression system towards SlIAA9 gene in tomato were successfully created. The strain of A. tumefaciens harboring pRGEB31- IAA9G2 plasmid was used to develop transgenic tomato plants from Micro-Tom variety. PCR test showed that 5/14 plants had the presence of Cas9 gene in T0 plants. The transgenic plants have a normal morphology in comparation with the controls. The evaluation of mutant efficiency, type, and stability of mutations on the SlIAA9 will be conducted on next-generation plants when the mutations are stable and segregated into descendents.

Identification of α-Tomatine 23-Hydroxylase Involved in the Detoxification of a Bitter Glycoalkaloid

2019 ◽  
Vol 61 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Masaru Nakayasu ◽  
Ryota Akiyama ◽  
Midori Kobayashi ◽  
Hyoung Jae Lee ◽  
Takashi Kawasaki ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Tomato Fruit ◽  
Transgenic Tomato ◽  
Metabolic Enzyme ◽  
Gene Encoding ◽  
Tomato Plants ◽  
Tomato Fruit Ripening ◽  
Transgenic Tomato Plants ◽  
Key Enzyme ◽  
Esculeoside A

Abstract Tomato plants (Solanum lycopersicum) contain steroidal glycoalkaloid α-tomatine, which functions as a chemical barrier to pathogens and predators. α-Tomatine accumulates in all tissues and at particularly high levels in leaves and immature green fruits. The compound is toxic and causes a bitter taste, but its presence decreases through metabolic conversion to nontoxic esculeoside A during fruit ripening. This study identifies the gene encoding a 23-hydroxylase of α-tomatine, which is a key to this process. Some 2-oxoglutarate-dependent dioxygenases were selected as candidates for the metabolic enzyme, and Solyc02g062460, designated Sl23DOX, was found to encode α-tomatine 23-hydroxylase. Biochemical analysis of the recombinant Sl23DOX protein demonstrated that it catalyzes the 23-hydroxylation of α-tomatine and the product spontaneously isomerizes to neorickiioside B, which is an intermediate in α-tomatine metabolism that appears during ripening. Leaves of transgenic tomato plants overexpressing Sl23DOX accumulated not only neorickiioside B but also another intermediate, lycoperoside C (23-O-acetylated neorickiioside B). Furthermore, the ripe fruits of Sl23DOX-silenced transgenic tomato plants contained lower levels of esculeoside A but substantially accumulated α-tomatine. Thus, Sl23DOX functions as α-tomatine 23-hydroxylase during the metabolic processing of toxic α-tomatine in tomato fruit ripening and is a key enzyme in the domestication of cultivated tomatoes.

scholarly journals Transgenic tomato plants expressing the antigen gene PfCP-2.9 of Plasmodium falciparum

2013 ◽  
Vol 48 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Mihail Kantor ◽  
Radu Sestras ◽  
Kamal Chowdhury
Keyword(s):  
Gene Expression ◽  
Plasmodium Falciparum ◽  
Agrobacterium Tumefaciens ◽  
Protein A ◽  
Transgenic Tomato ◽  
Pcr Analysis ◽  
Tomato Plants ◽  
Transgenic Expression ◽  
Transgenic Tomato Plants ◽  
Malaria Antigen

The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.

scholarly journals Expression of the Human Hepatitis B Virus Large Surface Antigen Gene in Transgenic Tomato Plants

2007 ◽  
Vol 14 (4) ◽  
pp. 464-469 ◽  
Author(s):  
Xiao-Ming Lou ◽  
Quan-Hong Yao ◽  
Zhen Zhang ◽  
Ri-He Peng ◽  
Ai-Sheng Xiong ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Tomato Fruit ◽  
Transgenic Tomato ◽  
Tomato Plants ◽  
Antigen Gene ◽  
Transgenic Tomato Plants ◽  
Pathogenesis Related Protein ◽  
B Virus

ABSTRACT The original hepatitis B virus (HBV) large surface antigen gene was synthesized. In order to optimize the expression of this gene in tomato plants, the tobacco pathogenesis-related protein S signal peptide was fused to the 5′ end of the modified gene and the sequence encoding amino acids S, E, K, D, E, and L was placed at the 3′ end. The gene encoding the modified HBV large surface antigen under the control of a fruit-specific promoter was constructed and expressed in transgenic tomato plants. The expression of the antigen from transgenic plants was confirmed by PCR and reverse transcriptase PCR. Enzyme-linked immunoassays using a monoclonal antibody directed against human serum-derived HBsAg revealed that the maximal level of HBsAg was about 0.02% of the soluble protein in transgenic tomato fruit. The amount of HBsAg in mature fruits was found to be 65- to 171-fold larger than in small or medium fruits and leaf tissues. Examination of transgenic plant samples by transmission electron microscopy proved that HBsAg had been expressed and had accumulated. The HBsAg protein was capable of assembling into capsomers and virus-like particles. To our knowledge, this is the first time the HBV large surface antigen has been expressed in plants. This work suggests the possibility of producing a new alternative vaccine for human HBV.

Studies on the Fungal Pathogen of Dutch Elm Disease

1981 ◽  
Vol 39 ◽  
pp. 400-401
Author(s):  
H.M. Mazzone ◽  
G. Wray ◽  
R. Zerillo
Keyword(s):  
Fungal Pathogen ◽  
Fungal Growth ◽  
Polymyxin B ◽  
The United States ◽  
Dutch Elm Disease ◽  
Effective Control ◽  
Sabouraud Agar ◽  
Total Inhibition ◽  
Zones Of Inhibition ◽  
Control Plate

The fungal pathogen of the Dutch elm disease (DED), Ceratocystis ulmi (Buisman) C. Moreau, has eluded effective control since its introduction in the United States more than sixty years ago. Our studies on DED include establishing biological control agents against C. ulmi. In this report we describe the inhibitory action of the antibiotic polymyxin B on the causal agent of DED.In screening a number of antibiotics against C. ulmi, we observed that filter paper discs containing 300 units (U) of polymyxin B (Difco Laboratories) per disc, produced zones of inhibition to the fungus grown on potato dextrose agar or Sabouraud agar plates (100mm x 15mm), Fig. 1a. Total inhibition of fungal growth on a plate occurred when agar overlays containing fungus and antibiotic (polymyxin B sulfate, ICN Pharmaceuticals, Inc.) were poured on the underlying agar growth medium. The agar overlays consisted of the following: 4.5 ml of 0.7% agar, 0.5 ml of fungus (control plate); 4.0 ml of 0.7% agar, 0.5 ml of fungus, 0.5 ml of polymyxin B sulfate (77,700 U). Fig. 1, b and c, compares a control plate and polymyxin plate after seven days.

Dark respiration rate of transgenic tomato plants expressing FeSOD1 gene under chloride and sulfate salinity

2014 ◽  
Vol 40 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Ye. N. Baranova ◽  
E. N. Akanov ◽  
A. A. Gulevich ◽  
L. V. Kurenina ◽  
S. A. Danilova ◽  
...  
Keyword(s):  
Respiration Rate ◽  
Dark Respiration ◽  
Transgenic Tomato ◽  
Dark Respiration Rate ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Genetic transactivation of Dissociation elements in transgenic tomato plants

1989 ◽  
Vol 218 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Michael W. Lassner ◽  
Joseph M. Palys ◽  
John I. Yoder
Keyword(s):  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Overexpression of LeNHX2 and SlSOS2 increases salt tolerance and fruit production in double transgenic tomato plants

2019 ◽  
Vol 135 ◽  
pp. 77-86 ◽  
Author(s):  
Mourad Baghour ◽  
Francisco Javier Gálvez ◽  
M. Elena Sánchez ◽  
M. Nieves Aranda ◽  
Kees Venema ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Fruit Production ◽  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

scholarly journals Control of Ethylene Synthesis by Expression of a Bacterial Enzyme in Transgenic Tomato Plants

1991 ◽  
Vol 3 (11) ◽  
pp. 1187 ◽  
Author(s):  
Harry J. Klee ◽  
Maria B. Hayford ◽  
Keith A. Kretzmer ◽  
Gerard F. Barry ◽  
Ganesh M. Kishore
Keyword(s):  
Transgenic Tomato ◽  
Ethylene Synthesis ◽  
Tomato Plants ◽  
Bacterial Enzyme ◽  
Transgenic Tomato Plants

scholarly journals Weather Variables Associated with Infection of Tomato Fruit by Colletotrichum coccodes

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 753-756 ◽  
Author(s):  
S. Sanogo ◽  
S. P. Pennypacker ◽  
R. E. Stevenson ◽  
A. A. MacNab
Keyword(s):  
Field Experiments ◽  
Tomato Fruit ◽  
Field Conditions ◽  
Colletotrichum Coccodes ◽  
Weather Variables ◽  
Tomato Plants ◽  
Growing Seasons ◽  
Central Pennsylvania ◽  
Best Fitting ◽  
Relationship Of

Field experiments were conducted to determine the relationship of tomato anthracnose to weather variables. Sixteen potted tomato plants were exposed to field conditions within rows of tomato plants for 4 consecutive days at various time periods during the 1993 and 1994 summer growing seasons. Incidence of fruit infection by Colletotrichum coccodes was correlated with rain variables (amount and duration of rain) alone and in combination with other meteorological factors. The best fitting regression equation, accounting for 72% of the variation in anthracnose incidence (arcsine-square root transformed), was Y = 111.77 - 1.16 HNRo, in which HNRo is the numbers of hours during which no rainfall occurs within 4-day intervals that tomato fruit were exposed to field conditions in central Pennsylvania.

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