Nitrogen Source Corrects a Potato-Dextrose Agar Medium Deficient in Supporting Mycelial Growth ofMoniliniaspp.

Plant Disease ◽  
1981 ◽  
Vol 65 (8) ◽  
pp. 649 ◽  
Author(s):  
P. L. Sholberg
Keyword(s):  
Nitrogen Source ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Potato Dextrose Agar Medium

scholarly journals Use of water restriction in the inoculation of carrot seeds

2019 ◽  
Vol 41 (4) ◽  
pp. 416-422
Author(s):  
Ricardo Borges Pereira ◽  
Tiago B. Torres ◽  
Patrícia Pereira da Silva ◽  
Gilvaine C. Lucas ◽  
Jadir Borges Pinheiro ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Osmotic Potential ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Control Treatment ◽  
Infection Rates ◽  
Water Restriction ◽  
Potato Dextrose Agar Medium ◽  
Osmotic Potentials

Abstract: This study aimed at evaluating the method of inoculation of carrot seeds in a potato-dextrose-agar medium (PDA) added of mannitol. Alternaria radicina was the fungus chosen to test such methodology. The osmosis-controlling agent was employed to obtain the osmotic potentials of 0.6 MPa, 0.8 MPa, 1.0 MPa and 1.2 MPa. In comparison with the control treatment, no significant mycelial growth inhibition was noticed at any of the osmotic potentials considered. The seeds were distributed over the fungal colonies for infection, where they remained for 48, 72, 96, 120 and 144 hours, at the osmotic potentials of 0.0 MPa (control), 0.6 MPa, 0.8 MPa, 1.0 MPa and 1.2 MPa, respectively. Tests of germination, sanitization and emergence were performed to evaluate the effects of colonization. Regarding the germination, the different osmotic potentials resulted in similar percentages of abnormal seedlings and dead seeds. As for the sanitization testing, higher infection rates were observed at the osmotic potentials of 1.0 MPa and 1.2 MPa. The lowest percentage of emergence was found at -1.2 MPa, therefore suggesting that this osmotic potential (produced with mannitol) might be used for infecting carrot seeds for research purposes.

scholarly journals Cultural variability among nine isolates of Urocystis agropyri on wheat

1970 ◽  
Vol 33 (3) ◽  
pp. 347-351
Author(s):  
SS Karwasra ◽  
MS Beniwal ◽  
ML Chhabra
Keyword(s):  
Key Words ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Corn Meal ◽  
Potato Dextrose Agar Medium ◽  
Flag Smut ◽  
Fast Growing ◽  
Different Temperatures ◽  
Slow Growing

Four temperatures and five different media were tested for cultural variability of nine geographical isolates of Urocystis agropyri (Preuss.) Schroet causing flag smut of wheat. Urocystis agropyri is extremely slow growing pathogen and each isolate responded differently at different temperatures. All the isolates/ collections grew well at 20°C after 45, 60, and 70 days after incubation. Ambala isolate was fast growing. PDA medium was found to be most suitable for the growth of all the nine isolates/collections. The average mycelial growth was also maximum on Potato Dextrose Agar Medium followed by Corn Meal Dextrose Agar, Oat Meal Dextrose Agar, Grain Meal Dextrose Agar and minimum on Wheat Meal Dextrose Agar. Key Words: Variability, media, isolates, wheat. doi:10.3329/bjar.v33i3.1593 Bangladesh J. Agril. Res. 33(3) : 347-351, September 2008

Antifungal Activity of 10-Oxo-trans-8-decenoic Acid and 1-Octen-3-ol against Penicillium expansum in Potato Dextrose Agar Medium

2003 ◽  
Vol 66 (8) ◽  
pp. 1503-1505 ◽  
Author(s):  
DERRICK O. OKULL ◽  
ROBERT B. BEELMAN ◽  
HASSAN GOURAMA
Keyword(s):  
Antifungal Activity ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Penicillium Expansum ◽  
Natural Food ◽  
Antifungal Compound ◽  
Decenoic Acid ◽  
Food Preservatives ◽  
Potato Dextrose Agar Medium

In mushrooms, 10-oxo-trans-8-decenoic acid (ODA) and 1-octen-3-ol are secondary metabolites produced naturally by the enzymatic breakdown of linoleic acid. Both compounds were determined to inhibit the mycelial growth of Penicillium expansum PP497A, a common food spoilage organism, when added to potato dextrose agar medium. ODA and 1-octen-3-ol were inhibitory at concentrations of >1.25 mM (230 μg/g for ODA and 160 μg/g for 1-octen-3-ol). At pH 5.6, 1-octen-3-ol was more inhibitory than ODA. However, at pH 3.5, both compounds (especially ODA) were more inhibitory than they were at pH 5.6. This finding indicates that the undissociated carboxyl of ODA was important for inhibition. At a concentration of 2.5 mM and a pH of 3.5, ODA and 1-octen-3-ol inhibited growth by 43.1 and 41.9%, respectively. An additive effect was observed when both compounds were added at a combined concentration of ≥1.25 mM; when both were added at a combined concentration of 2.5 mM, mycelial growth was inhibited by 48.8 and 72.8% at pHs of 5.6 and 3.5, respectively. Although the antifungal activity levels for these two compounds were lower than those observed for equal molar concentrations of sorbate, a common antifungal compound, these findings indicate that further investigation of the potential of ODA and 1-octen-3-ol for use as natural food preservatives is warranted.

scholarly journals Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

2001 ◽  
Vol 58 (3) ◽  
pp. 613-616 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Cindia Mara Miniuk ◽  
Neiva Monteiro de Barros ◽  
João Lúcio Azevedo
Keyword(s):  
Biological Control ◽  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Continuous Illumination ◽  
Agricultural Pests ◽  
Dark Cycle ◽  
Metarhizium Flavoviride ◽  
Conidia Production

Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05).

scholarly journals Dataset of differentially accumulated proteins in Mucor strains representative of four species grown on synthetic potato dextrose agar medium and a cheese mimicking medium

Data in Brief ◽  
2017 ◽  
Vol 11 ◽  
pp. 214-220 ◽  
Author(s):  
Stéphanie Morin-Sardin ◽  
Jean-Luc Jany ◽  
Sébastien Artigaud ◽  
Vianney Pichereau ◽  
Benoît Bernay ◽  
...  
Keyword(s):  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Potato Dextrose Agar Medium

scholarly journals Vascular Streak Dieback: Penyakit Baru Tanaman Kakao di Sumatera Barat

2016 ◽  
Vol 12 (4) ◽  
pp. 142
Author(s):  
Jumsu Trisno ◽  
Reflin Reflin ◽  
Martinius Martinius
Keyword(s):  
Agar Medium ◽  
Disease Incidence ◽  
Infected Plant ◽  
Potato Dextrose Agar ◽  
Fungal Isolation ◽  
Potato Dextrose Agar Medium ◽  
First Report ◽  
Plant Samples ◽  
Dead Plant

Vascular streak dieback (VSD) symptoms was reported recently in several cacao plantations in West Sumatera.  Disease incidence reached 58.82–100% with disease intensity of 24.29–44.71%.  In some cases, dead plant was also found. Fungal isolation was performed to identify the agents associated with VSD.  Plant samples showing VSD symptoms was collected from 3 locations of cacao production center in West Sumatera, i.e. Limapuluh Kota regency, Padang Pariaman regency, and Padang city.  Small pieces of leaf and twig were plated on water agar and potato dextrose agar medium for fungal isolation.  Morphology of hifa, basidiocarp, and basidiospora observed from fungi colonies indicated the presence of Ceratobasidium theobromae on infected plant samples.  This is the first report on the association of C. theobromae on cacao in West Sumatera. 

scholarly journals Hirsutella thompsonii and Pochonia chlamydosporia (Syn. Verticillium chlamydosporium) Mycelia Growth and Predation on Panagrellus redivivus

2017 ◽  
Vol 9 (11) ◽  
pp. 137
Author(s):  
Cleonice Lubian ◽  
Danielle Dutra Matinha ◽  
Roberto Luis Portz ◽  
Vivian Carré Missio ◽  
Luanna Karoline Rinaldi ◽  
...  
Keyword(s):  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Free Living ◽  
Potato Dextrose Agar Medium ◽  
Panagrellus Redivivus ◽  
Evaluation Time ◽  
Mycelia Growth ◽  
Hirsutella Thompsonii ◽  
Low Levels ◽  
Verticillium Chlamydosporium

This research aimed to evaluate the nematophagous ability of 4077-Verticillium chlamydosporium var. chlamydosporium and 4466-Hirsutella thompsonii isolates and relate mycelia growth to the influence provoked by movement of nematodes. Each fungus grew in PDA (potato, dextrose, agar) medium end up to pure colonization. Then, ten mycelia plugs of 8 mm diameter were removed from colony borders and transferred to the center of ten Petri plates containing water-agar 2% medium. These plates were previously divided into four quadrants that received a number of 25 individuals of free-living nematodes (Panagrellus redivivus), composing a total of 100 nematodes per plate. Evaluations started after 24 hours of interaction, considering predation percentage and mycelia growth as stimuli of nematodes presence. Results showed growing predation performance to both isolates, being higher for V. chlamydosporium var. chlamydosporium since from first evaluation time, controlling more than 50% of nematode population initially added. Its predation potential was 39.2%, 38.4% and 48.35% higher than H. thompsonii at first, second and third evaluation day, respectively. Generally, nematodes did not stimulate mycelia growth, unless for H. thompsonii at 72 hours of interaction compared to control plates (without nematodes). Stress resulting from isolates transference from PDA to water-agar 2% resulted in sparse mycelia growth and it could have affected the predation performance of H. thompsonii that controlled nematodes in low levels throughout experiment. Independently of predation level, pictures revealed that both isolates has ability to control P. redivivus through hyphae penetration.

scholarly journals Screening of Effective Media for the Growth of Pyricularia grisea under In-vitro Condition

2021 ◽  
Vol 9 (3) ◽  
pp. 131-135
Author(s):  
Ajay Singh Kaurav ◽  
Keyword(s):  
Pearl Millet ◽  
Mycelial Growth ◽  
Agar Medium ◽  
Warm Season ◽  
Potato Dextrose Agar ◽  
Annual Rainfall ◽  
Pyricularia Grisea ◽  
Cereal Crop ◽  
Effective Media

Pearl millet [Pennisetum glaucoma (L.) R. Br.], belongs to family Poaceae (section Paniceae), is the world’s hardiest warm-season cereal crop with the annual rainfall of 150 mm to 1000 mm.Maximum mycelial growth was recorded in potato dextrose carrot agar medium (86.33 mm) followed by Pearl millet Potato dextrose agar (83.67 mm), potato dextrose agar (78.33 mm), Pearl millet carrot dextrose agar (71.67 mm), Pearl millet potato agar (68.33 mm), Carrot dextrose agar (60.67 mm), Pearl millet grain potato dextrose agar (58.33 mm), Oat meal agar (51.67 mm), Pearl millet dextrose agar (51.67 mm), Calcium carbonate agar (42.67 mm), Malt agar (41.00 mm), Water agar (37.33 mm), Pearl millet grain dextrose agar (35.33 mm), Pearl millet agar (30.00) and Yeast extract agar (28.33 mm) while minimum growth was recorded in Pearl millet grain agar (27.33 mm).

scholarly journals Incidence and Biology of Corynespora cassiicola (Berk. & Curt.) Wei. disease of okra in Bangladesh

2014 ◽  
Vol 42 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Fakhruddin Ali Ahmed ◽  
Nazmul Alam ◽  
Abul Khair
Keyword(s):  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Corynespora Cassiicola ◽  
Optimum Growth ◽  
Potato Dextrose Agar Medium ◽  
Significant Incidence ◽  
Effects Of Temperature ◽  
A Minor

Eight commercial okra cultivars were evaluated to determine the magnitude of incidence of Corynespora cassiicola (Berk. & Curt.) Wei. Maximum and significant incidence of C. cassiicola was 14.86 in the cultivar BARI 1. In rest of the cultivars, the occurrence of this fungus was very poor to nil. Corynespora cassiicola appeared to be a minor pathogen for okra. Effects of temperature, pH and culture media on growth and sporulation of the fungus were investigated. Potato dextrose agar medium was found to be the most suitable for optimum growth and sporulation of this fungus at pH 7 and 25ºC. The conidial length significantly varied with the changes of pH. The correlation between pH and conidial length was found negative and highly significant. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18028 Bangladesh J. Bot. 42(2): 265-272, 2013 (December)

scholarly journals Sclerotinia Rot of Peucedanum japonicum Thunb. Caused by Sclerotinia sclerotiorum

2021 ◽  
Vol 27 (3) ◽  
pp. 115-119
Author(s):  
Won-Kwon Jung ◽  
Yang-Sook Lim ◽  
Min-Ki Kim ◽  
Jong-Su Kim
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Brown Spot ◽  
Cylindrical Shape ◽  
Potato Dextrose Agar Medium ◽  
Partial Sequencing ◽  
Typical Symptom ◽  
First Report ◽  
Sclerotinia Rot

Sclerotinia rot was occurred on the leaf and stem of Peucedanum japonicum Thunb. in greenhouse field of Pohang city of Gyeongbuk province in Korea. The typical symptom of the disease was light brown spot and tipburn on infected leaves. The colony of the isolated fungus was white to light gray in color. Asci were cylindrical shape and 75‒240×5.9‒17.3 μm in size. Apothecia were cup-shaped with numerous asci and 0.5‒0.9 cm in size. Ascospores were aseptate and ellipsoid in shape, and 8.4‒10.7×4.8‒5.8 μm in size. Sclerotia formed on the plants and potato dextrose agar medium were globose to irregular in shape and black in color. Partial sequencing of rDNA of this isolate showed that it was 100% consistent with that of Sclerotinia sclerotiorum. It was confirmed that the same lesion was formed by reinoculating this pathogen on a healthy P. japonicum Thunb. and the same strain was isolated. This is the first report on the Sclerotinia rot of P. japonicum Thunb. caused by S. sclerotiorum in Korea.

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