scholarly journals Identification and Characterization of Pestalotioid Fungi Causing Leaf Spots on Mango in Southern China

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1207-1213 ◽  
Author(s):  
Juan Shu ◽  
Zhihe Yu ◽  
Wenxiu Sun ◽  
Jiang Zhao ◽  
Qili Li ◽  
...  
Keyword(s):  
Southern China ◽  
Elongation Factor ◽  
Morphological Characterization ◽  
Morphological Data ◽  
Pathogenicity Test ◽  
Species Identity ◽  
Translation Elongation ◽  
Leaf Spots ◽  
Mango Leaves

Mango is an economically important fruit crop in southern China. However, leaf spots restrict the development of mango trees, reducing the yield and production. Pestalotioid fungi are one of the major agents causing leaf spots on mango. During 2016 and 2017, 21 isolates of pestalotioid fungi associated with leaf spots on mango leaves were collected from five provinces in southern China: Guangxi, Hainan, Yunnan, Guangdong, and Fujian. All 21 isolates were subjected to morphological characterization and DNA sequence analysis. The morphological data were combined with analyses of concatenated sequences of the ITS (internal transcribed spacer), TEF 1-α (translation elongation factor), and TUB2 (β-tubulin) for higher resolution of the species identity of these isolates. The results showed that these isolates belong to Neopestalotiopsis clavispora, Pestalotiopsis adusta, P. anacardiacearum, P. asiatica, P. photinicola, P. saprophyta, P. trachicarpicola, and Pseudopestalotiopsis ampullacea. Pathogenicity test results showed that all these species could cause symptoms. On detached mango leaves (cv. Tainong), early foliar symptoms on leaves were small yellow-to-brown lesions. Later, these spots expanded with uneven borders, turned white to gray, and coalesced to form larger gray patches. To our knowledge, this is the first description of N. clavispora, P. adusta, P. asiatica, P. photinicola, P. saprophyta, P. trachicarpicola, or Ps. ampullacea as causal agents for leaf spots on mango worldwide.

scholarly journals Genealogical concordance of phylogenetic species recognition-based delimitation of Neopestalotiopsis species associated with leaf spots and fruit canker disease affected guava plants

2021 ◽  
Vol 58 (04) ◽  
pp. 1301-1313
Author(s):  
Imran Ul Haq
Keyword(s):  
Species Recognition ◽  
Elongation Factor ◽  
Plant Diseases ◽  
Morphological Data ◽  
Phylogenetic Species ◽  
Translation Elongation ◽  
Canker Disease ◽  
Leaf Spots ◽  
Genealogical Concordance ◽  
Elongation Factor 1

Neopestalotiopsis species are known to be involved in plant diseases as associated pathogens. The taxonomic identification of the fungal group Neopestalotiopsisis little bit difficult due to its complex evolutionary history. In the present study, seven fungal isolates were investigated from canker-affected guava plants. The phylogeny for generic placement of these isolates was analyzed to validate them as Neopestalotiopsis genus by phylogenetic signals from the 28S nrRNA region (LSU). Generated morphological data was segregated as new morpho-species of the Neopestalotiopsis genus. Hence, the internal transcribed spacer (ITS), Translation elongation factor 1-α (TEF1-α) and Tubulin (TUB) genic region of these isolates were studied in juxtaposition with morphological data to resolve species limits. Both phylogenetic and morphological data revealed four novel species of the Neopestalotiopsis genus out of seven isolates studied. These Neopestalotiopsis species could be of great significance for further investigation as putative pathogens associated with canker or scabby canker disease in guava.

scholarly journals Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 788
Author(s):  
Saida S. Gerardo-Lugo ◽  
Juan M. Tovar-Pedraza ◽  
Sajeewa S. N. Maharachchikumbura ◽  
Miguel A. Apodaca-Sánchez ◽  
Kamila C. Correia ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Leaf Spot ◽  
Elongation Factor ◽  
Its Region ◽  
Morphological Characterization ◽  
Leaf Spot Disease ◽  
Grey Leaf Spot ◽  
Spot Disease ◽  
Leaf Spots ◽  
Mango Leaves

Mango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed in two main production areas in Sinaloa State of Mexico, and 50 Neopestalotiopsis isolates were obtained. Fungal identification of 20 representative isolates was performed using morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (TEF) and the β-tubulin (TUB) genes. Phylogenetic analysis indicated that the 20 isolates from this study formed four consistent groups, however, overall tree topologies do not consistently provide a stable and sufficient resolution. Therefore, even though morphological and phylogenetic separation is evident, these isolates were not assigned to any new taxa and were tentatively placed into four clades (clades A–D). Pathogenicity tests on detached mango leaves of cv. Kent showed that the 20 isolates that belong to the four Neopestalotiopsis clades from this study and induce lesions on mango leaves. This is the first report of species of Neopestalotiopsis causing mango grey leaf spot disease in Mexico.

scholarly journals Morphological Characterization of Cephaleuros virescens Occurring in Mango Trees

2019 ◽  
Vol 11 (11) ◽  
pp. 156
Author(s):  
Camila Vilela Vasconcelos ◽  
Paulo Henrique Pereira Costa Muniz ◽  
Elizabeth Amélia Alves Duarte ◽  
Thiago Alves Santos de Oliveira ◽  
Wanderson Silva dos Santos ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Morphological Characterization ◽  
Causal Agent ◽  
Morphological Data ◽  
Reproductive Structures ◽  
Mango Leaves ◽  
Microscope Slides

The objective of this work was to accomplish the micromorphological characterization of the causal agent of the algal spot in ‘Amrapali’ mango trees. Therefore, ‘Amrapali’ mango leaves exhibiting symptoms of algal spots were used for making microscope slides, on which the pathogen somatic and reproductive structures were measured. The dimensions obtained for sporangiophores and sporangia were 245.5-545.6 × 10.5-19.1 μm and 21.4-34.2 × 16.3-24.7 μm, respectively. Descriptions based on symptoms and micromorphology indicated that the algae are Cephaleuros virescens, since this morphological data corroborates with literature descriptions. Besides, the algae had already identified by DNA sequencing as C. virescens.

scholarly journals Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2083-2100 ◽  
Author(s):  
Beatriz Mora-Sala ◽  
Ana Cabral ◽  
Maela León ◽  
Carlos Agustí-Brisach ◽  
Josep Armengol ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Elongation Factor ◽  
Its Region ◽  
Phylogenetic Position ◽  
Translation Elongation ◽  
Forest Nurseries ◽  
Asexual Morphs ◽  
Identification And Characterization ◽  
Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

A new species of Scleroramularia associated with sooty blotch and flyspeck in Southern China

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 261 ◽  
Author(s):  
Liu Gao ◽  
Zhuoya Wei ◽  
Bo Wang ◽  
Mark L. Gleason ◽  
Rong Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Elongation Factor ◽  
Space Region ◽  
Translation Elongation ◽  
Sooty Blotch ◽  
Elongation Factor 1 Alpha ◽  
Sooty Blotch And Flyspeck ◽  
Elongation Factor 1 ◽  
A New Species

Scleroramularia is a genus that includes species of the sooty blotch and flyspeck (SBFS) fungal complex that blemishes the surface of apple, pawpaw, and other fruit. In a survey of SBFS on banana (Musa basjoo), an isolate associated with the flyspeck mycelial type of SBFS was obtained from Zhanjiang, Guangdong, China. Based on analysis of morphology and phylogeny (the nuclear ribosomal internal transcribed space region and translation elongation factor 1-alpha), it was delimited as a new species of Scleroramularia, described here as S. musae. Conidia of S. musae have more septa than other species presently known in the genus.

A new species of Heimioporus (Boletaceae) from southern China

Phytotaxa ◽  
2019 ◽  
Vol 415 (4) ◽  
pp. 179-188
Author(s):  
XIANG-NYU CHEN ◽  
MING ZHANG ◽  
TAI-HUI LI ◽  
NIAN-KAI ZENG
Keyword(s):  
New Species ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
Distinct Member ◽  
Elongation Factor 1 ◽  
A New Species

Heimioporus sinensis, collected from tropical and subtropical areas of China, is introduced as a new species based on both morphological characters and molecular data. The species is characterized by the purplish red to deep magenta pileus, the reticulated stipe, the irregularly reticulate to reticulate-alveolate basidiospores 11.5–13.5 × 8–9.5 μm, and a trichodermal to intricately trichodermal pileipellis. Phylogenetic analyses based on the nuc 28S rDNA D1-D2 domains (28S) and the translation elongation factor 1-α gene (tef1-α) showed that H. sinensis is a distinct member of the genus Heimioporus in the subfamily Xerocomoideae.

scholarly journals Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines

Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1678-1688 ◽  
Author(s):  
Antonia Carlucci ◽  
Francesca Cibelli ◽  
Francesco Lops ◽  
Maria Luisa Raimondo
Keyword(s):  
Elongation Factor ◽  
Phylogenetic Study ◽  
Translation Elongation ◽  
Neofusicoccum Parvum ◽  
Grapevine Trunk Diseases ◽  
General Decline ◽  
Wide Range ◽  
Trunk Diseases ◽  
Pathogenicity Tests

Botryosphaeriaceae spp. have a cosmopolitan distribution and a wide range of plant hosts. Over the last 15 years, worldwide, 21 species of this family have been associated with grapevine trunk diseases that cause cankers and dieback on grapevines. Here, we surveyed vineyards of Vitis vinifera ‘Lambrusco’, ‘Sangiovese’, and ‘Montepulciano’ in three areas of the Foggia province (Cerignola, Foggia, and San Severo) in southern Italy. Wood samples from grapevines showing general decline, dieback, cankers, and wood and foliar discoloration yielded 344 fungal isolates identified as Botryosphaeriaceae spp. A phylogenetic study combining internal transcribed spacer and translation elongation factor 1-α sequences of 60 representative isolates identified nine botryosphaeriaceous species: Botryosphaeria dothidea, Diplodia corticola, D. mutila, D. seriata, Dothiorella iberica, Do. sarmentorum, Lasiodiplodia citricola, L. theobromae, and Neofusicoccum parvum. Pathogenicity tests confirmed that all nine species cause canker and dieback of grapevines. However, this is the first report of L. citricola as causal agent of wood cankers and dieback of grapevine. To date, including L. citricola, there are 25 botryosphaeriaceous species associated with V. vinifera worldwide, of which 12 have been reported for grapevines in Italy.

Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov.

Phytotaxa ◽  
2021 ◽  
Vol 479 (1) ◽  
pp. 23-43
Author(s):  
NARUEMON HUANALUEK ◽  
RUVISHIKA S. JAYAWARDENA ◽  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
DULANJALEE L. HARISHCHANDRA
Keyword(s):  
Elongation Factor ◽  
Climatic Conditions ◽  
Annona Squamosa ◽  
Translation Elongation Factor ◽  
Garcinia Mangostana ◽  
Translation Elongation ◽  
Leaf Spots ◽  
First Time ◽  
Elongation Factor 1 ◽  
Partial Translation

Pestalotioid fungi commonly occur as pathogens, endophytes or saprobes. In this study, pestalotioid fungi associated with leaf spots and fruit rots were isolated from Alpinia malaccensis, A. galangal, Annona squamosa, Artocarpus heterophyllus, Citrus sp., Garcinia mangostana, Litsea petiolata, a pteridophyte, and Vitis vinifera in Chiang Rai, Thailand. Based on single- and multi-locus phylogenies using internal transcribed spacer, β-tubulin and partial translation elongation factor 1-α gene regions, along with morphological features, the isolates were identified as two new species, Neopestalotiopsis hydeana and Pestalotiopsis hydei. This is the first time a Pestalotiopsis sp. was reported associated with Litsea petiolata and a Neopestalotiopsis sp. recorded from Alpinia, Annona, Artocarpus, Garcinia and a pteridophyte in the world. This fungal group can be considered as an emerging pathogenic group on different hosts in different climatic conditions. 

A new species and a new combination in the genus Aureoboletus (Boletales, Boletaceae) from southern China

Phytotaxa ◽  
2015 ◽  
Vol 222 (2) ◽  
pp. 129 ◽  
Author(s):  
Nian-Kai Zeng ◽  
MING ZHANG ◽  
ZHI-QUN Liang
Keyword(s):  
Rna Polymerase Ii ◽  
Southern China ◽  
Large Subunit ◽  
Line Drawing ◽  
Elongation Factor ◽  
Molecular Data ◽  
New Combination ◽  
Translation Elongation ◽  
Microscopic Features ◽  
A New Species

Two lineages of Aureoboletus (Boletales, Boletaceae) from southern China were revealed by using molecular data based on combined dataset of the nuclear ribosomal large subunit RNA (nrLSU), the translation elongation factor apha-1 (tef1-a) and the largest subunit of RNA polymerase II (rpb1). One of them corresponds with the previous morphology-based taxon, viz. Boletellus longicollis, another one is different from those taxa described based on morphological features. And, thus, Auroboletus clavatus sp. nov. and A. longicollis comb. nov. were proposed. A detailed description, colour photos of fresh basidiomata, and a line-drawing of microscopic features of the two taxa were provided.

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