scholarly journals Development of a Simple Hydroponic Assay to Study Vertical and Horizontal Resistance of Soybean and Pathotypes of Phytophthora sojae

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 114-123 ◽  
Author(s):  
A. Lebreton ◽  
C. Labbé ◽  
M. De Ronne ◽  
A. G. Xue ◽  
G. Marchand ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Root Rot ◽  
Dry Weight ◽  
Phytophthora Sojae ◽  
Horizontal Resistance ◽  
Phytophthora Root Rot ◽  
Hydroponic System ◽  
Long Term Stability ◽  
Vertical Resistance

Phytophthora root rot, caused by Phytophthora sojae, is one of the most damaging diseases of soybean and the introgression of Rps (Resistance to P. sojae) genes into elite soybean lines is arguably the best way to manage this disease. Current bioassays to phenotype the gene-for-gene relationship are hampered with respect to reproducibility and long-term stability of isolates, and do not accurately predict horizontal resistance individually. The aim of our study was to investigate a new way of phenotyping P. sojae isolates and vertical and horizontal resistance in soybean that relies on zoospores inoculated directly into a hydroponic system. Inoculation of P. sojae isolates against a set of eight differentials accurately and reproducibly identified pathotypes over a period of two years. When applied to test vertical resistance of soybean lines with known and unknown Rps genes, the bioassay relied on plant dry weight to correctly identify all genes. In addition, simultaneous inoculations of three P. sojae isolates, collectively carrying eight major virulence factors against 64 soybean lines with known and unknown levels of horizontal resistance, separated the plants into five distinct groups of root rot, allowing the discrimination of lines with various degrees of partial resistance. Based on those results, this bioassay offers several advantages in facilitating efforts in breeding soybean for P. sojae resistance and in identifying virulence factors in P. sojae.

scholarly journals Influence of Pure Mulches on Suppressing Phytophthora Root Rot Pathogens

2013 ◽  
Vol 31 (4) ◽  
pp. 221-226 ◽  
Author(s):  
G.C. Percival
Keyword(s):  
Root Rot ◽  
Crop Production ◽  
Prunus Avium ◽  
European Beech ◽  
Dry Weight ◽  
Urban Trees ◽  
Horse Chestnut ◽  
Phytophthora Root Rot ◽  
Tree Vitality ◽  
Lesion Severity

Mulching as a means of controlling Phytophthora root rot pathogens has become recognised as a potential cultural management system within the arboricultural, nursery and landscape industry. The influence of a pure mulch, i.e., mulch derived solely from one tree species, on reducing Phytophthora root rot severity has received little study. The purpose of the conducted research was to determine if a range of pure mulches derived from European beech (Fagus sylvatica L.), common hawthorn (Crataegus monogyna JACQ), silver birch (Betula pendula ROTH.), common cherry (Prunus avium L.), evergreen oak (Quercus ilex L.) and English oak (Q. robur L.) could reduce the development and impact of pathogen severity caused by Phytophthora cactorum and P. criticola on containerised horse chestnut (Aesculus hippocastanum). Irrespective of Phytophthora pathogen, leaf area, leaf, shoot, root and total plant dry weight following application of a pure mulch was higher than non-mulched controls. Likewise, leaf chlorophyll content, chlorophyll fluorescence Fv/Fm ratios, photosynthetic rates and root carbohydrate concentration as measures of tree vitality were higher in pure mulched compared to non-mulched control trees. Application of a pure mulch had a significant influence on Phytophthora root rot lesion severity. In the case of P. cactorum root rot lesion severity was reduced by 39–63%. In the case of P. criticola root rot lesion severity was reduced by 33–61%. In conclusion, pure mulches offer positive benefits for those involved in the care and maintenance of urban trees as well as nursery, forestry, orchard and horticultural crop production where Phytophthora pathogens are problematic.

scholarly journals Ornamental Cherry Tolerance of Flooding and Phytophthora Root Rot

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 988-991 ◽  
Author(s):  
K.A. Jacobs ◽  
G.R. Johnson
Keyword(s):  
Survival Rate ◽  
Root Rot ◽  
Net Photosynthesis ◽  
Severity Index ◽  
Individual Treatment ◽  
Control Group ◽  
Term Survival ◽  
Phytophthora Root Rot ◽  
Long Term Survival

Seedlings of eight Prunus taxa were evaluated for variation in susceptibility to a single, 4- or 5-day flooding period and root rot caused by Phytophthora cryptogea Pethybr. & Lafferty. Survival, plant defoliation, disease severity index, root necrosis, and net photosynthesis indicated that the combination of flooding and pathogen was significantly more severe to all taxa than either individual treatment. Most response variables reflected early plant dysfunction but were not correlated with long-term survival. Long-term survival was 70% in the combination treatment compared to 99% in the control group. Flooding injured seedlings more than the pathogen in most taxa. Taxa differed only slightly in tolerance to the treatments, as measured by survival rate. Prunus takesimensis Nakai had the highest survival rate of 100% and along with P. mahaleb L. and P. yedoensis Matsum. showed some tolerance to flooding and the pathogen. Prunus sargentii Rehd. had the lowest survival rate of 81% and appeared to be least tolerant to the pathogen.

Comparison of phosetyl-Al, phosphorous acid and metalaxyl for the long-term control of Phytophthora root rot of avocado

1987 ◽  
Vol 27 (3) ◽  
pp. 471 ◽  
Author(s):  
KG Pegg ◽  
AW Whiley ◽  
PW Langdon ◽  
JB Saranah
Keyword(s):  
Root Rot ◽  
Phytophthora Cinnamomi ◽  
Phosphorous Acid ◽  
Growing Season ◽  
Phytophthora Root Rot ◽  
The Third ◽  
Consecutive Season

Avocado trees affected by root rot caused by Phytophthora cinnamomi Rands recovered rapidly when given injections into the trunk of phosetyl-A1 or phosphorous acid. Injected trees had greater yields(47.9-67.5 v. 4.3 kg fruit per tree) and lower leaf chloride levels (0.8-2.4 v. 4.2%, w/w) than controls in the third season after starting treatment. Phosphorous acid residues (21-83 mg kg-1) were detected in fruit from injected trees. Metalaxyl applied to the soil twice each growing season successfully controlled root rot for the first 2 seasons, but there was a significant resurgence of decline symptoms after the third consecutive season of use.

Detached-petiole inoculation method to evaluate Phytophthora root rot resistance in soybean plants

2017 ◽  
Vol 68 (6) ◽  
pp. 555
Author(s):  
Yinping Li ◽  
Suli Sun ◽  
Chao Zhong ◽  
Zhendong Zhu
Keyword(s):  
Root Rot ◽  
Dominant Gene ◽  
Phytophthora Sojae ◽  
Single Dominant Gene ◽  
Phytophthora Root Rot ◽  
Inoculation Methods ◽  
Inoculation Technique ◽  
Soybean Cultivars ◽  
F2 Population ◽  
Soybean Resistance

Phytophthora root rot (PRR) caused by Phytophthora sojae, is one of the most destructive soybean diseases. The deployment of resistant cultivars is an important disease management strategy. To this aim, the development of a fast and effective method to evaluate soybean resistance to P. sojae is strategic. In this study, a detached-petiole inoculation technique was developed and its reliability was verified in soybean cultivars and segregant populations for PRR resistance. The detached-petiole and hypocotyl inoculation methods were used to assess the resistance of soybean cultivars, the F2 population of a Zhonghuang47 × Xiu94-11 cross, and the derived F2:3 population. The reactions of 13 analysed cultivars to three P. sojae isolates were consistent between the two inoculation techniques. The reactions of the F2 and F2:3 populations to isolate PsMC1 were 95.20% similar between the two inoculation methods. The segregation of the resistance and susceptibility fit a 3 : 1 ratio. Our results suggest that the detached-petiole technique is a reliable method, and reveal that the PRR resistance in Xiu94-11 is controlled by a single dominant gene. The phenotypic ratios of the tested Jikedou2 × Qichadou1 F2 population using the detached-petiole inoculation technique fit a 3 : 1 ratio (Resistance : Susceptibility). This demonstrated that Qichadou1 contains a single dominant gene conferring resistance to P. sojae. Our new detached-petiole inoculation technique is effective, reliable, non-destructive to the plant, and does not require an excessive amount of seeds. It may be suitable for the largescale screening of soybean resistance to multiple P. sojae isolates.

scholarly journals Adaptation of Phytophthora sojae to Rps Resistance Genes over the Past Two Decades in North Dakota

2019 ◽  
Vol 20 (2) ◽  
pp. 88-93 ◽  
Author(s):  
Hui Yan ◽  
Berlin Nelson
Keyword(s):  
North Dakota ◽  
Resistance Genes ◽  
Root Rot ◽  
Phytophthora Sojae ◽  
Red River ◽  
Phytophthora Root Rot ◽  
Red River Valley ◽  
The Past ◽  
Primary Management ◽  
New Strategies

Phytophthora root rot, caused by Phytophthora sojae, is a major disease of soybean in North Dakota, especially in the Red River Valley (RRV). Planting resistant cultivars is the primary management. The resistance genes Rps 1c, 1k, 3a, and 6 are the most common genes deployed in this region. To determine the efficacy of these genes and document the pathotype changes in the population of P. sojae over several decades, a survey of pathotypes was conducted in 2015 in three counties in the southern RRV and compared with similar surveys conducted in 1991 to 1994 and 2002 to 2004 in the same area. The results showed that from 1991 to 1994 when 6% of the pathotypes could defeat the Rps1c gene, by 2004 it was 57% of the pathotypes, and that percentage remained the same in 2015. However, in 1994 no pathotype could defeat Rps 1k, but by 2004 it was 12% and in 2015 it was 41%. Pathotypes that defeat Rps 3a and 6 have been few over the years. Pathotypes that defeat both 1c and 1k increased from none to 31% between 1994 and 2015. With the increasing complexity of P. sojae pathotypes, new strategies for managing this pathogen in the future will be needed.

Races of Phytophthora sojae on Soybean in Illinois

2000 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
R. A. Leitz ◽  
G. L. Hartman ◽  
W. L. Pedersen ◽  
C. D. Nickell
Keyword(s):  
United States ◽  
Glycine Max ◽  
Root Rot ◽  
The United States ◽  
Phytophthora Sojae ◽  
Phytophthora Root Rot ◽  
Glycine Max L

Phytophthora root rot of soybean (Glycine max (L.) Merr.), caused by Phytophthora sojae M. J. Kauffmann & J. W. Gerdemann, has been isolated throughout the soybean-producing regions of the United States. Posted 3 June 2000.

scholarly journals Development and Application of qPCR and RPA Genus- and Species-Specific Detection of Phytophthora sojae and P. sansomeana Root Rot Pathogens of Soybean

Plant Disease ◽  
2017 ◽  
Vol 101 (7) ◽  
pp. 1171-1181 ◽  
Author(s):  
J. Alejandro Rojas ◽  
Timothy D. Miles ◽  
Michael D. Coffey ◽  
Frank N. Martin ◽  
Martin I. Chilvers
Keyword(s):  
Mitochondrial Genome ◽  
Internal Control ◽  
Root Rot ◽  
Phytophthora Sojae ◽  
Diagnostic Tools ◽  
Specific Detection ◽  
Phytophthora Root Rot ◽  
Phytophthora Spp ◽  
Field Season ◽  
Species Specific

Phytophthora root rot of soybean, caused by Phytophthora sojae, is one of the most important diseases in the Midwestern United States, and is estimated to cause losses of up to 1.2 million metric tons per year. Disease may also be caused by P. sansomeana; however, the prevalence and damage caused by this species is not well known, partly due to limitations of current diagnostic tools. Efficient, accurate, and sensitive detection of pathogens is crucial for management. Thus, multiplex qPCR and isothermal RPA (recombinase polymerase amplification) assays were developed using a hierarchical approach to detect these Phytophthora spp. The assays consist of a genus-specific probe and two species-specific probes that target the atp9-nad9 region of the mitochondrial genome that is highly specific for the genus Phytophthora. The qPCR approach multiplexes the three probes and a plant internal control. The RPA assays run each probe independently with a plant internal control multiplexed in one amplification, obtaining a result in as little as 20 mins. The multicopy mitochondrial genome provides sensitivity with sufficient variability to discern among different Phytophthora spp. The assays were highly specific when tested against a panel of 100 Phytophthora taxa and range of Pythium spp. The consistent detection level of the assay was 100 fg for the qPCR assay and 10 pg for the RPA assay. The assays were validated on symptomatic plants collected from Michigan (U.S.) and Ontario (Canada) during the 2013 field season, showing correlation with isolation. In 2014, the assays were validated with samples from nine soybean producing states in the U.S. The assays are valuable diagnostic tools for detection of Phytophthora spp. affecting soybean.

scholarly journals New Oomycota Fungicides With Activity Against Phytophthora cinnamomi and Their Potential Use for Managing Avocado Root Rot in California

Plant Disease ◽  
2019 ◽  
Vol 103 (8) ◽  
pp. 2024-2032 ◽  
Author(s):  
Rodger J. Belisle ◽  
Wei Hao ◽  
Brandon McKee ◽  
Mary Lu Arpaia ◽  
Patricia Manosalva ◽  
...  
Keyword(s):  
Root Rot ◽  
Phytophthora Cinnamomi ◽  
Dry Weight ◽  
The United States ◽  
Baseline Sensitivity ◽  
Phytophthora Root Rot ◽  
Mean Values ◽  
Potassium Phosphite ◽  
Pathogen Populations

Phytophthora root rot (PRR), caused by Phytophthora cinnamomi, is the most destructive disease of avocado worldwide. In the United States, mefenoxam and phosphonate products are currently the only registered fungicides for managing avocado PRR. Four new Oomycota-specific and two registered fungicides, all with different modes of action, were evaluated. Seventy-one isolates of P. cinnamomi from avocado in California, most of them collected between 2009 to 2017, were tested for their in vitro sensitivity to the six fungicides. Baseline sensitivity ranges and mean values (in parentheses) of effective concentrations to inhibit mycelial growth by 50% (EC50) for the new fungicides ethaboxam, fluopicolide, mandipropamid, and oxathiapiprolin were 0.017 to 0.069 μg/ml (0.035), 0.046 to 0.330 μg/ml (0.133), 0.003 to 0.011 μg/ml (0.005), and 0.0002 to 0.0007 μg/ml (0.0004), respectively. In comparison, the EC50 value range (mean) was 0.023 to 0.138 μg/ml (0.061) for mefenoxam and 12.9 to 361.2 μg/ml (81.5) for potassium phosphite. Greenhouse soil inoculation trials with 8-month-old Zutano seedlings and 10-month-old Dusa and PS.54 clonal rootstocks were conducted to assess the efficacy of these fungicides for managing PRR. Mefenoxam and potassium phosphite were effective treatments; however, oxathiapiprolin, fluopicolide, and mandipropamid were more effective. Ethaboxam was effective in reducing PRR on the rootstocks evaluated. Oxathiapiprolin reduced PRR incidence and pathogen population size in the soil by >90%, and plant shoot growth and root dry weight were significantly increased compared with the control; thus, oxathiapiprolin was one of the best treatments overall. The high activity and performance of these new fungicides supports their registrations on avocado for use in rotation and mixture programs, including with previously registered compounds, to reduce the risk of development and spread of resistance in pathogen populations.

scholarly journals Races of Phytophthora sojae on Soybean in Illinois

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 487-487 ◽  
Author(s):  
R. A. Leitz ◽  
G. L. Hartman ◽  
W. L. Pedersen ◽  
C. D. Nickell
Keyword(s):  
Root Rot ◽  
Genetic Resistance ◽  
The United States ◽  
Phytophthora Sojae ◽  
Diseased Plant ◽  
American Phytopathological Society ◽  
Phytophthora Root Rot ◽  
Glycine Max L ◽  
New Race ◽  
Soybean Diseases

Phytophthora root rot of soybean (Glycine max (L.) Merr.), caused by Phytophthora sojae M. J. Kauffmann & J. W. Gerdemann, has been isolated throughout the soybean-producing regions of the United States. There are more than 39 identified races of P. sojae pathogenic on soybean, and 13 host resistance alleles have been identified at 7 loci (1). None of these alleles confers resistance to all races of P. sojae. The most commonly used resistance allele, Rps1k, confers resistance to the greatest number of races (2). The objective of this study was to identify races of P. sojae in Illinois soybean fields to determine whether the currently used resistance alleles are effective against the P. sojae races found in Illinois. Soybean breeders must be aware of the existence and distribution of races to incorporate appropriate sources of genetic resistance into cultivars. From 192 soil samples collected throughout Illinois in 1997, 33 isolates were obtained and identified to race by inoculating Rps isolines of soybean cv. Williams. A new race with virulence to the Rps1d and Rps7 alleles, designated as race 54, accounted for 48% of the isolates. Another new race with virulence to Rps1d, Rps3a, Rps3c, Rps4, Rps5, Rps6, and Rps7 alleles, designated race 55, was identified in one sample. One isolate, identified as race 41, was obtained from a diseased plant with the Rps1k allele. Another isolate, identified as race 43, was obtained from a diseased plant with the Rps1c allele. Based on virulence patterns of P. sojae, most of the isolates obtained from Illinois soils were races 1, 3, and 4 or variants of these races, such as race 54, with added virulence to the Rps1d allele. References: (1) A. F. Schmitthenner. 1999. Compendium of Soybean Diseases. 4th ed. G. L. Hartman, J. B. Sinclair, and J. C. Rupe, eds. The American Phytopathological Society, St. Paul, MN. pp. 39‐42. (2) A. F. Schmitthenner, M. Hobe, and R. G. Bhat. Plant Dis. 78:269, 1994

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