Genetic trends in Fusarium head blight resistance due to 20 years of winter wheat breeding and cooperative testing in the Northern US.

Plant Disease ◽  
2021 ◽  
Author(s):  
Rupesh Gaire ◽  
Clay Sneller ◽  
Gina Brown-Guedira ◽  
David A. Van Sanford ◽  
Mohsen Mohammadi ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Mixed Model ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Vital Role ◽  
Head Blight ◽  
Genetic Progress ◽  
Fhb Resistance ◽  
Genetic Trends

Fusarium head blight (FHB) is a devastating disease of wheat and barley. In the US, a significant long-term investment in breeding FHB resistant cultivars began after the 1990s. However, to this date, no study has been performed to understand and monitor the rate of genetic progress in FHB resistance as a result of this investment. Using 20 years of data (1998 to 2018) from the Northern Uniform (NU) and Preliminarily Northern Uniform (PNU) winter wheat scab nurseries which consisted of 1068 genotypes originating from 9 different institutions, we studied the genetic trends in FHB resistance within the northern soft red winter wheat growing region using mixed model analyses. For the FHB resistance traits incidence, severity, Fusarium damaged kernels (FDK), and deoxynivalenol content, the rate of genetic gain in disease resistance was estimated to be 0.30 ± 0.1, 0.60 ± 0.09, 0.37 ± 0.11 points per year, and 0.11 ± 0.05 ppm per year, respectively. Among the five FHB resistance QTL assayed for test entries from 2012 to 2018, the frequencies of favorable alleles from Fhb 2DL Wuhan1 W14, Fhb Ernie 3Bc, and Fhb 5A Ning7840 was close to zero across the years. The frequency of the favorable at Fhb1 and Fhb 5A Ernie ranged from 0.08 to 0.33 and 0.06 to 0.20 respectively across years, and there was no trend in changes in allele frequencies over years. Overall, this study showed that substantial genetic progress has been made towards improving resistance to FHB. It is apparent that the current investment in public wheat breeding for FHB resistance is achieving results and will continue to play a vital role in reducing FHB levels in growers’ fields.

scholarly journals Alien chromatin but not Fhb7 confers Fusarium head blight resistance in wheat breeding

2021 ◽  
Author(s):  
Xianrui Guo ◽  
Qinghua Shi ◽  
Jing Yuan ◽  
Mian Wang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Glutathione S Transferase ◽  
Head Blight ◽  
Fhb Resistance ◽  
Translocation Lines ◽  
High Level ◽  
Thinopyrum Elongatum

AbstractFusarium head blight (FHB), caused by Fusarium species, seriously threaten global wheat production. Three wheat-Th.elongatum FHB resistant translocation lines have been developed and used for breeding. Transcriptomic analysis identified a derivative glutathione S-transferase transcript T26102, which was homologous to Fhb7 and induced dramatically by Fusarium graminearum. Homologs of Fhb7 were detected in several genera in Triticeae, including Thinopyrum, Elymus, Leymus, Pseudoroegeria and Roegeria. Several wheat-Thinopyrum translocation lines carrying Fhb7 remain susceptible to FHB, and transgenic plants overexpressing the T26102 on different backgrounds did not improve the FHB resistance. Taken as a whole, we show the application of the chromatin derived from diploid Thinopyrum elongatum successfully conferring wheat with high level FHB resistance independent of the Fhb7.One Sentence SummaryThinopyrum elongatum chromatin from 7EL was successfully applied to wheat FHB resistance breeding, but the resistant gene other than the reported Fhb7 remained unknown.

scholarly journals Identification of a Novel Genomic Region Associated With Resistance to Fusarium Head Blight in Chinese Winter Wheat

2021 ◽  
Author(s):  
Yunzhe Zhao ◽  
Xinying Zhao ◽  
Mengqi Ji ◽  
Wenqi Fang ◽  
Hong Guo ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Genome Wide Association Study ◽  
Wheat Breeding ◽  
Scab Resistance ◽  
Yield Reduction ◽  
Transferase Activity ◽  
Head Blight ◽  
A Genome ◽  
Fhb Resistance

Abstract Background: Fusarium head blight (FHB) is a disease affecting wheat spikes caused by Fusarium species, which leads to cases of severe yield reduction and seed contamination. Therefore, identifying resistance genes from various sources is always of importance to wheat breeders. In this study, a genome-wide association study (GWAS) focusing on FHB using a high-density genetic map constructed with 90K single nucleotide polymorphism (SNP) arrays in a panel of 205 elite winter wheat accessions, was conducted in 3 environments. Results: Sixty-six significant marker–trait associations (MTAs) were identified (P<0.001) on fifteen chromosomes explaining 5.4–11.2% of the phenotypic variation therein. Some important new genomic regions involving FHB resistance were found on chromosomes 2A, 3B, 5B, 6A, and 7B. On chromosome 7B, 6 MTAs at 92 genetic positions were found in 2 environments. Moreover, there were 11 MTAs consistently associated with diseased spikelet rate and diseased rachis rate as pleiotropic effect loci. Eight new candidate genes of FHB resistance were predicated in wheat. Of which, three genes: TraesCS5D01G006700, TraesCS6A02G013600, and TraesCS7B02G370700 on chromosome 5DS, 6AS, and 7BL, respectively, were important in defending against FHB by regulating chitinase activity, calcium ion binding, intramolecular transferase activity, and UDP-glycosyltransferase activity in wheat. In addition, a total of six excellent alleles associated with wheat scab resistance were discovered. Conclusion: These results provide important genes/loci for enhancing FHB resistance in wheat breeding populations by marker-assisted selection.

scholarly journals A Quantitative Proteomics View on the Function of Qfhb1, a Major QTL for Fusarium Head Blight Resistance in Wheat

Pathogens ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 58 ◽  
Author(s):  
Moustafa Eldakak ◽  
Aayudh Das ◽  
Yongbin Zhuang ◽  
Jai Rohila ◽  
Karl Glover ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Starch Synthesis ◽  
Resistance Mechanism ◽  
Fusarium Head Blight Resistance ◽  
Defense Responses ◽  
Wheat Breeding ◽  
Host Cells ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus

Fusarium head blight (FHB) is a highly detrimental disease of wheat. A quantitative trait locus for FHB resistance, Qfhb1, is the most utilized source of resistance in wheat-breeding programs, but very little is known about its resistance mechanism. In this study, we elucidated a prospective FHB resistance mechanism by investigating the proteomic signatures of Qfhb1 in a pair of contrasting wheat near-isogenic lines (NIL) after 24 h of inoculation of wheat florets by Fusarium graminearum. Statistical comparisons of the abundances of protein spots on the 2D-DIGE gels of contrasting NILs (fhb1+ NIL = Qfhb1 present; fhb1- NIL = Qfhb1 absent) enabled us to select 80 high-ranking differentially accumulated protein (DAP) spots. An additional evaluation confirmed that the DAP spots were specific to the spikelet from fhb1- NIL (50 spots), and fhb1+ NIL (seven spots). The proteomic data also suggest that the absence of Qfhb1 makes the fhb1- NIL vulnerable to Fusarium attack by constitutively impairing several mechanisms including sucrose homeostasis by enhancing starch synthesis from sucrose. In the absence of Qfhb1, Fusarium inoculations severely damaged photosynthetic machinery; altered the metabolism of carbohydrates, nitrogen and phenylpropanoids; disrupted the balance of proton gradients across relevant membranes; disturbed the homeostasis of many important signaling molecules induced the mobility of cellular repair; and reduced translational activities. These changes in the fhb1- NIL led to strong defense responses centered on the hypersensitive response (HSR), resulting in infected cells suicide and the consequent initiation of FHB development. Therefore, the results of this study suggest that Qfhb1 largely functions to either alleviate HSR or to manipulate the host cells to not respond to Fusarium infection.

scholarly journals Characterizing Winter Wheat Germplasm for Fusarium Head Blight Resistance Under Accelerated Growth Conditions

2021 ◽  
Vol 12 ◽  
Author(s):  
Mustafa Zakieh ◽  
David S. Gaikpa ◽  
Fernanda Leiva Sandoval ◽  
Marwan Alamrani ◽  
Tina Henriksson ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Growth Conditions ◽  
Head Blight ◽  
Wheat Germplasm ◽  
Progress Curve ◽  
Accelerated Growth ◽  
Fhb Resistance

Fusarium head blight (FHB) is one of the economically important diseases of wheat as it causes severe yield loss and reduces grain quality. In winter wheat, due to its vernalization requirement, it takes an exceptionally long time for plants to reach the heading stage, thereby prolonging the time it takes for characterizing germplasm for FHB resistance. Therefore, in this work, we developed a protocol to evaluate winter wheat germplasm for FHB resistance under accelerated growth conditions. The protocol reduces the time required for plants to begin heading while avoiding any visible symptoms of stress on plants. The protocol was tested on 432 genotypes obtained from a breeding program and a genebank. The mean area under disease progress curve for FHB was 225.13 in the breeding set and 195.53 in the genebank set, indicating that the germplasm from the genebank set had higher resistance to FHB. In total, 10 quantitative trait loci (QTL) for FHB severity were identified by association mapping. Of these, nine QTL were identified in the combined set comprising both genebank and breeding sets, while two QTL each were identified in the breeding set and genebank set, respectively, when analyzed separately. Some QTLs overlapped between the three datasets. The results reveal that the protocol for FHB evaluation integrating accelerated growth conditions is an efficient approach for FHB resistance breeding in winter wheat and can be even applied to spring wheat after minor modifications.

scholarly journals Mapping of Major Fusarium Head Blight Resistance from Canadian Wheat cv. AAC Tenacious

2020 ◽  
Vol 21 (12) ◽  
pp. 4497 ◽  
Author(s):  
Raman Dhariwal ◽  
Maria A. Henriquez ◽  
Colin Hiebert ◽  
Curt A. McCartney ◽  
Harpinder S. Randhawa
Keyword(s):  
Fusarium Head Blight ◽  
Agronomic Traits ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Head Blight ◽  
Visual Rating ◽  
Wheat Population ◽  
Major Bottleneck ◽  
Yield Quality ◽  
Fhb Resistance

Fusarium head blight (FHB) is one of the most devastating wheat disease due to its direct detrimental effects on grain-yield, quality and marketability. Resistant cultivars offer the most effective approach to manage FHB; however, the lack of different resistance resources is still a major bottleneck for wheat breeding programs. To identify and dissect FHB resistance, a doubled haploid wheat population produced from the Canadian spring wheat cvs AAC Innova and AAC Tenacious was phenotyped for FHB response variables incidence and severity, visual rating index (VRI), deoxynivalenol (DON) content, and agronomic traits days to anthesis (DTA) and plant height (PHT), followed by single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) marker genotyping. A high-density map was constructed consisting of 10,328 markers, mapped on all 21 chromosomes with a map density of 0.35 cM/marker. Together, two major quantitative trait loci for FHB resistance were identified on chromosome 2D from AAC Tenacious; one of these loci on 2DS also colocated with loci for DTA and PHT. Another major locus for PHT, which cosegregates with locus for low DON, was also identified along with many minor and epistatic loci. QTL identified from AAC Tenacious may be useful to pyramid FHB resistance.

scholarly journals Effectiveness of multigenerational transfer of Sumai 3 Fusarium head blight resistance in hard red spring wheat breeding populations

2020 ◽  
Vol 100 (2) ◽  
pp. 156-174
Author(s):  
S. Berraies ◽  
R.E. Knox ◽  
R.M. DePauw ◽  
F.R. Clarke ◽  
A.R. Martin ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Type I ◽  
Head Blight ◽  
Hard Red Spring Wheat ◽  
Breeding Populations ◽  
Fhb Resistance ◽  
Selection For ◽  
Sumai 3

Several quantitative trait loci (QTL) have been identified for Fusarium head blight (FHB) resistance in the cultivar Sumai 3. Wheat breeders need to know which Sumai 3 loci are present in derived lines used as parents for effective marker-assisted selection for genetic improvement. This study was conducted to identify the loci in Sumai 3 derived parents that contribute FHB resistance in breeding populations. Three doubled haploid (DH) populations utilizing Sumai 3 derived parents, ND3085, ND744, and Alsen, were evaluated during 2007 and 2008 in FHB nurseries near Carman, MB, Ottawa, ON and Charlottetown, PE. The percentage of incidence, severity, Fusarium-damaged kernels (FDK), and deoxynivalenol (DON) accumulation were measured, and FHB index calculated. DNA markers at six FHB resistance loci detected in Sumai 3 were evaluated on the populations. For each trait, a t test was applied to means of observations pooled by parental type of each marker to determine which loci contributed to resistance. The alleles at 3BS and 5AS most frequently contributed to Type I and Type II FHB resistance, as well as to reduced FDK and DON in all three populations. Markers revealed resistance on 3BS and 5AS in Alsen, ND3085, and ND744, on 3BSc, 4D, and 6BS in ND744, on 4D in ND3085, and on 6BS in Alsen. In some environments, the susceptible parent Infinity contributed minor QTL on 2D, 3BSc, and 6BS. Likewise, Helios contributed minor QTL on 5AS and 6BS.

scholarly journals Exploring Fusarium Head Blight Resistance In a Winter Triticale Germplasm Collection

2021 ◽  
Author(s):  
Sydney Wallace ◽  
Bhavit Chhabra ◽  
Yanhong Dong ◽  
Xuefeng Ma ◽  
Gary Coleman ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Genetic Resistance ◽  
Fusarium Head Blight Resistance ◽  
Germplasm Collection ◽  
Wheat Breeding ◽  
Head Blight ◽  
Winter Triticale ◽  
New Varieties ◽  
Fhb Resistance ◽  
Grain Yield And Quality

Fusarium Head Blight (FHB) is a destructive disease affecting the grain yield and quality of wheat, barley, rye and triticale. Developing varieties with genetic resistance is integral to successfully managing FHB. However, significant knowledge gap exists in the genetic diversity present in triticale for FHB resistance. This information is critical for breeding new varieties of triticale as its production continues to increase. In the present study, a set of 298 winter triticale accessions from a worldwide collection were screened for their type-2 FHB resistance in an artificially inoculated misted nursery with high levels of inoculum density. Most of the triticale accessions were susceptible to FHB, and only 8% of accessions showed resistance in the field nursery screening. The resistant accessions identified in the nursery screening were selected and further screened for three years in greenhouse conditions. Seven accessions were found to show robust FHB resistance over the three years of greenhouse testing. Thirteen accessions showed significantly lower levels of Deoxynivalenol accumulation when compared to the susceptible triticale control. The accessions identified in the study will be useful in triticale and wheat breeding programs for enhancing FHB resistance and reducing DON accumulation.

scholarly journals Molecular Cytogenetic Characterization and Fusarium Head Blight Resistance of Five Wheat-Thinopyrum intermedium Partial Amphiploids

2021 ◽  
Author(s):  
Hui Wang ◽  
Shuwei Cheng ◽  
Yue Shi ◽  
Shuxin Zhang ◽  
Wei Yan ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Thinopyrum Intermedium ◽  
Head Blight ◽  
Multicolor Fish ◽  
Wheat Diseases ◽  
Cytogenetic Characterization ◽  
Fhb Resistance ◽  
Partial Amphiploids

Abstract Background: Partial amphiploids created by crossing octoploid tritelytrigia（2n=8x=56, AABBDDEE） and Thinopyrum intermedium (2n=6x=42, StStJJJSJS) are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat-Th. intermedium partial amphiploids using GISH and multicolor-FISH. Results: The result revealed that five lines had 10-14 J-genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J-genomic DNA from Th. bessarabicum as GISH probe and the oligo probes pAs1-1, pAs1-3, AFA-4, (GAA) 10, and pSc119.2-1 as FISH probe. Five lines resembled their parent octoploid tritelytrigia （2n=8x=56, AABBDDEE） but had higher protein contents. Protein contents of two lines HS2-2 and HS2-5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2-2, HS2-4, HS2-5, and HS2-16 were less than 20%. Line HS2-5 with 14 J-genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively. Conclusions: New wheat-Th. intermedium amphiploids with the J-genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.

scholarly journals Introgression breeding - Effects and side effects of marker-based introduction of two non-adapted QTL for Fusarium head blight resistance into elite wheat

2011 ◽  
Vol 63 (1) ◽  
pp. 129-136
Author(s):  
T. Miedaner ◽  
C. von der Ohe ◽  
V. Korzun ◽  
E. Ebmeyer
Keyword(s):  
Side Effects ◽  
Winter Wheat ◽  
Grain Yield ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Recurrent Parent ◽  
Head Blight ◽  
Introgression Breeding ◽  
Fhb Resistance

Introgression breeding - Effects and side effects of marker-based introduction of two non-adaptedQTLfor Fusarium head blight resistance into elite wheatFusarium head blight resistance (FHB) can be achieved by using improved adapted varieties as crossing partners or by a marker-assisted introgression of mapped QTL from non-adapted sources. In this long-term studyFhb1on chromosome 3BS andQfhs.ifa-5Alocated on chromosome 5A were introgressed into European elite spring and winter wheat to test effects on FHB resistance and side effects on agronomic performance andF. graminearumisolates and mixtures. The introgression of the QTLFhb1andQfhs.ifa-5Afrom the Sumai 3-descendant CM82036 could be performed in the shortest possible way by marker-assisted backcrossing. They were both validated in European elite wheat background. Effects on FHB resistance were, on average, only about half of the effect in the original mapping populations. In the best phenotypically and marker-selected BC0line of spring wheat FHB was reduced from 40 to 4.3% of disease symptoms by introgressing both QTL, in the best BC3line of winter wheat the reduction was 28 and 37% for the moderately resistant and highly susceptible recurrent parent, respectively. Introgression of both QTL simultaneously did not result in significantly higher FHB resistance than introgression of only one of both QTL. Small significant negative effects on grain yield were detected in the Anthus but not in the Opus BC3F2:5backcross population when both QTL were introgressed. Backcrossing with onlyQfhs.ifa-5Adid not reduce grain yield significantly. Differences in heading date, plant height and quality traits were in all cases small although often significant. Selection of lines with improved resistance level and similar high yield level like the recurrent parent was feasible. Stability of FHB resistance mediated by both QTL was stable across 22Fusariumisolates from Europe and Canada and six binary mixtures. Competition effects between isolates in mixtures were obvious but could not be attributed to the resistance of the host. In conclusion, marker-based backcrossing is a feasible option for introgressingFhb1orQfhs.ifa-5AQTL into the high-yielding, quality-oriented European wheat gene pool.

The effect of 3BS locus of Sumai 3 on Fusarium head blight resistance in Australian wheats

2007 ◽  
Vol 47 (5) ◽  
pp. 603 ◽  
Author(s):  
G. Q. Xie ◽  
M. C. Zhang ◽  
S. Chakraborty ◽  
C. J. Liu
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Head Blight ◽  
Breeding Programs ◽  
Backcross Populations ◽  
Australian Wheat ◽  
Fhb Resistance ◽  
Sumai 3

The 3BS allele of Sumai 3 has been the main source of Fusarium head blight (FHB) resistance worldwide. Using molecular markers and FHB resistance screenings, we have analysed the effects of this allele in two backcross and two 4-way F2 populations derived from elite Australian cultivars. Compared to individuals without the Sumai 3 allele, individuals with the allele showed an average 32.0% reduction in FHB severity as measured by number of diseased spikelets. This value was slightly reduced to 29.2% when the total number of spikelets was taken into account by expressing severity as the proportion of diseased spikelets. When compared to the parental cultivars, progeny with the 3BS allele of Sumai 3 offered, on average, 43.3% reduction in FHB severity. Significant differences were not detected between progeny that were homozygous or heterozygous for the 3BS locus, indicating a dominant inheritance of this locus. These results confirm that the 3BS allele controls a large component of the FHB resistance in Sumai 3, which can be readily incorporated and detected in backcross populations using molecular markers. The materials derived from this study could offer significant benefits to the Australian wheat breeding programs.

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