Molecular, phenotypical, and host-range characterization of Robbsia andropogonis strains isolated from Bougainvillea spp. in Mexico.

Plant Disease ◽  
2021 ◽  
Author(s):  
Oscar Morales-Galván ◽  
Deepti Singh ◽  
Anthony Joseph Young ◽  
Alexander Ignatov ◽  
Dimas Mejia-Sanchez ◽  
...  
Keyword(s):  
Zea Mays ◽  
Hordeum Vulgare ◽  
Plant Protection ◽  
Gene Sequencing ◽  
Bacterial Isolates ◽  
Mexican Lime ◽  
Protection Strategies ◽  
Range Characterization ◽  
Phenotypic Tests

Characteristic leaf spot and blight symptoms caused by Robbsia andropogonis on bougainvillea plants were found in three locations in different provinces of Mexico from 2019 to 2020. Eleven bacterial isolates with morphology similar to R. andropogonis were obtained from the diseased bougainvillea leaves. The isolates were confirmed as R. andropogonis by phenotypic tests and 16S rRNA, rpoD and gyrB gene sequencing. In addition to bougainvillea, the strains were pathogenic to multiple agriculturally significant crops, including maize (Zea mays), sorghum (Sorghum bicolor), barley (Hordeum vulgare), coffee (Coffea arabiga), carnation (Dianthus caryophilus), Mexican lime (Citrus x aurantifolia), common bean (Phaseolus vulgaris), broadbeans (Vicia faba) and pea (Pisum sativum), but not runner bean (Phaseolus coccineus). The presence of this bacterium represents a challenge for plant protection strategies in Mexico.

scholarly journals Molecular, Physiological, and Host-Range Characterization of Acidovorax avenae subsp. citrulli Isolates from Watermelon and Melon in Israel

Plant Disease ◽  
2005 ◽  
Vol 89 (12) ◽  
pp. 1339-1347 ◽  
Author(s):  
Saul Burdman ◽  
Nadia Kots ◽  
Giora Kritzman ◽  
June Kopelowitz
Keyword(s):  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Pulse Field ◽  
Bacterial Isolates ◽  
Pulse Field Gel Electrophoresis ◽  
Acid Methyl ◽  
Group I ◽  
Serious Disease ◽  
Range Characterization

Bacterial fruit blotch (BFB), caused by Acidovorax avenae subsp. citrulli, is a serious disease of cucurbit plants. The first important occurrence of BFB in Israel was during 2000 to 2003 on watermelon and melon. Twelve bacterial isolates associated with these outbreaks were confirmed as A. avenae subsp. citrulli by pathogenicity assays, gas chromatography of fatty-acid methyl esters, and substrate-utilization profiles. The isolates were characterized in terms of their aggressiveness in different hosts by seed, seedling, and fruit inoculations, and according to their DNA fingerprinting profiles using pulse-field gel electrophoresis (PFGE) and repetitive-PCR approaches. Results from the present work agree with previous studies supporting the existence of two differentiated groups within A. avenae subsp. citrulli, one including strains that are more associated with watermelon (group II), the other consisting of strains that are usually associated with nonwatermelon cucurbits (group I). This study indicates that isolates from both groups have been introduced to Israel. PFGE analysis revealed that the 12 analyzed isolates can be divided into five different haplotypes, of which four were previously unreported. Additional differentiating features between group I and II strains are presented.

Ultrastructure of Plant Leaf Tissue Infected with Mite-Borne Viral-Like Pathogens

1970 ◽  
Vol 28 ◽  
pp. 178-179 ◽  
Author(s):  
O. E. Bradfute ◽  
R. E. Whitmoyer ◽  
L. R. Nault
Keyword(s):  
Triticum Aestivum ◽  
Zea Mays ◽  
Electron Microscope ◽  
Hordeum Vulgare ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Leaf Tissue ◽  
Leaf Ultrastructure ◽  
Double Membrane ◽  
Aceria Tulipae

A pathogen transmitted by the eriophyid mite, Aceria tulipae, infects a number of Gramineae producing symptoms similar to wheat spot mosaic virus (1). An electron microscope study of leaf ultrastructure from systemically infected Zea mays, Hordeum vulgare, and Triticum aestivum showed the presence of ovoid, double membrane bodies (0.1 - 0.2 microns) in the cytoplasm of parenchyma, phloem and epidermis cells (Fig. 1 ).

scholarly journals Extraction Techniques and Analytical Methods for Characterization of Active Compounds in Origanum Species

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4735
Author(s):  
Maša Knez Hrnčič ◽  
Darija Cör ◽  
Jana Simonovska ◽  
Željko Knez ◽  
Zoran Kavrakovski ◽  
...  
Keyword(s):  
Biological Activity ◽  
Abiotic Stresses ◽  
Plant Protection ◽  
Analytical Techniques ◽  
Extraction Techniques ◽  
Isolation And Identification ◽  
Activity Assessment ◽  
Current Contribution ◽  
Pharmaceutical Industries

Phytochemical research based on ethnopharmacology is gaining interest in industries such as functional food, nutraceuticals, cosmetics and pharmaceutical industries. Plants and plant extracts are a rich source of bioactive secondary metabolites. These compounds are often involved in plant protection against biotic or abiotic stresses. The exploitation of available technologies should be oriented and intensified to extend and enhance the continued usefulness of the plants as renewable sources of chemicals, especially medicinal compounds. This current contribution is focused on extraction and analytical techniques for their isolation from the oregano species, their characterization and their potential antioxidative, as well as their antimicrobial, antifungal and anticarcinogenic properties. The work is structured rendering to the different steps involved in the research; starting with extraction and sample preparation, followed by discussing the analytical techniques employed for the isolation and identification of compound/s responsible for the biological activity and methods and techniques for biological activity assessment.

Characterization of physiological and molecular responses of Zea mays seedlings to different urea-ammonium ratios

2021 ◽  
Vol 162 ◽  
pp. 613-623 ◽  
Author(s):  
Sara Buoso ◽  
Nicola Tomasi ◽  
Daniel Said-Pullicino ◽  
Mustapha Arkoun ◽  
Jean-Claude Yvin ◽  
...  
Keyword(s):  
Zea Mays ◽  
Molecular Responses
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