scholarly journals Bacterial Streak and Bulb Rot of Onion: I. A Diagnostic Medium for the Semiselective Isolation and Enumeration of Pseudomonas viridiflava

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 897-900 ◽  
Author(s):  
R. Gitaitis ◽  
D. Sumner ◽  
D. Gay ◽  
D. Smittle ◽  
G. McDonald ◽  
...  

A semiselective, diagnostic agar medium (T-5) and low temperature incubation technique were developed for recovering Pseudomonas viridiflava from the environment or plant material. Medium T-5 contains the following per liter: NaCl, 5.0 g; NH4H2PO4, 1.0 g; K2HPO4, 1.0 g; MgSO4·H2O, 0.2 g; D-tartaric acid, 3.0 g; phenol red, 0.01 g; agar, 20.0 g; bacitracin, 10 mg; vancomycin, 6 mg; cycloheximide, 75 mg; novobiocin, 45 mg; penicillin G, 5 mg. The pH is adjusted to 7.4. Antibiotics are added aseptically after autoclaving. P. viridiflava recovery from artificially infested, field-soil (Tifton loamy-sand), with a cropping history of no onion production, was high, with a corresponding reduction of 99.99% of nontarget bacteria. However, soils from fields with a long history of onion production, near Vidalia, Georgia, contained significantly larger populations of background microflora that grew on medium T-5. Incubation at 5°C reduced contaminating microflora 1,000- to 10,000-fold with no reduction in recovery of the target organism. However, this low temperature incubation required an increased incubation period of 3 weeks and reduced the level of fluorescence of P. viridiflava.

2014 ◽  
Vol 11 (2) ◽  
pp. 79-91
Author(s):  
Barbara Łacicowa ◽  
Zofia Machowicz ◽  
Danuta Sułek

Bean seeds were analyzed for infection by <i>Colletotrichum lindemuthianum</i> and fungi of the genus <i>Fusarium</i> using the method of isolation on maltose medium and by means of planting the seeds in moist sand. 38 samplesof seed from various varieties of beans were examined. Both methods proved to be suitable for the detection of fungi, but the percentage of seeds infected by <i>Colletotrichum lindemuthianum</i> and fungi of the <i>Fusarium</i> genus can be estimated more precisely by determining the number of infected seedlings obtained from material planted in the sand. On the agar medium used the growth of <i>Colletotrichum lindemuthanum</i> and fungi from the <i>Fusarium</i> genus was impossible due to other fungi present in the plant material. Treatment of the bean seeds with a low temperature (-16ºC for 12 hours) in necessary before using the method of isolation on maltose medium.


Plant Disease ◽  
1998 ◽  
Vol 82 (8) ◽  
pp. 935-938 ◽  
Author(s):  
R. Gitaitis ◽  
G. MacDonald ◽  
R. Torrance ◽  
R. Hartley ◽  
D. R. Sumner ◽  
...  

A semiselective agar medium (T-5) was used to sample environment and plant material as a habitat for Pseudomonas viridiflava. Soil, irrigation water, soybean foliage, and foliage from various weeds in an onion-growing area were assayed over a 5-year period. P. viridiflava was recovered only as an epiphytic resident from weed species that included cutleaf evening primrose, dandelion, common fumitory, purple cudweed, spiny sowthistle, Virginia pepperweed, and wild radish. The bacterium apparently overseasons in association with these weeds, because its presence was detected prior to, as well as during, the onion-growing season. Preliminary tests indicated that weed control may suppress bacterial streak and bulb rot levels.


1995 ◽  
Vol 31 (12) ◽  
pp. 239-248 ◽  
Author(s):  
Ana Emparanza-Knörr ◽  
Francisco Torrella

The Salmonella presence and the microbiological quality indicators, total and fecal coliforms and coliphages of E. coli C, have been studied in a overloaded wastewater lagoon system treating urban wastewatrers of the village of Guardamar del Segura (Alicante, Spain). Classical microbiological technology to detect salmonellae was used, including pre-enrichment, enrichment, selective media plating and biochemical and serological confirmation. Water was physicochemically characterized according to COD, SS, temperature, pH and dissolved oxygen. The selective migration step through Rappaport-Vassiliadis semisolid agar medium was essential for the consistent detection of Salmonella in the different lagoon effluents. Total and fecal coliform levels of up to 105-106 MPN/100 ml were detected in the final effluent. High coliphage concentrations of 103-104 pfu/ml were also found in the effluent waters. Salmonella was always detected in 100 ml samples and eventually reached an order of value of 103 MPN/100 ml. Total coliform reduction was higher in the anaerobic ponds whereas fecal coliforms were more efficiently eliminated in the facultative (mostly “anoxic”) lagoons. Coliphage reduction was higher in the facultative lagoons when compared to the anaerobic ponds. On many occasions, no reduction or eventual increment of the concentration of salmonellae was detected in the effluents from the anaerobic ponds compared to concentrations of the patohogen in the influent raw wasterwaters. The possibility exists for a capacity of Salmonella to multiply in the anoxic phase of the wastewater treatment, but the presence of microorganisms in raw sewage waters which could maskSalmonella detection with the enrichment methodology employed cannot be ruled out.


2019 ◽  
Vol 84 (3) ◽  
pp. 400-419 ◽  
Author(s):  
Molly Carney ◽  
Jade d'Alpoim Guedes ◽  
Kevin J. Lyons ◽  
Melissa Goodman Elgar

This project considered the deposition history of a burned structure located on the Kalispel Tribe of Indians ancestral lands at the Flying Goose site in northeastern Washington. Excavation of the structure revealed stratified deposits that do not conform to established Columbia Plateau architectural types. The small size, location, and absence of artifacts lead us to hypothesize that this site was once a non-domestic structure. We tested this hypothesis with paleoethnobotanical, bulk geoarchaeological, thin section, and experimental firing data to deduce the structural remains and the post-occupation sequence. The structure burned at a relatively low temperature, was buried soon afterward with imported rubified sediment, and was exposed to seasonal river inundation. Subsequently, a second fire consumed a unique assemblage of plant remains. Drawing on recent approaches to structured deposition and historic processes, we incorporate ethnography to argue that this structure was a menstrual lodge. These structures are common in ethnographic descriptions, although no menstrual lodges have been positively identified in the archaeological record of the North American Pacific Northwest. This interpretation is important to understanding the development and time depth of gendered practices of Interior Northwest groups.


Catalysts ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 100 ◽  
Author(s):  
Ke Zhang ◽  
Feng Yu ◽  
Mingyuan Zhu ◽  
Jianming Dan ◽  
Xugen Wang ◽  
...  
Keyword(s):  

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Hae Won Jung ◽  
Chong Rae Cho ◽  
Ji Yoon Ryoo ◽  
Hyun Kyo Lee ◽  
So Young Ha ◽  
...  

Actinomyces meyeriis an uncommon cause of human actinomycosis. Here, we report a rare case of empyema caused byA. meyeri. A 49-year-old male presented with a history of 10 days of dyspnea and chest pain. A large amount of loculated pleural effusion was present on the right side and multiple lung nodules were documented on radiological studies. A chest tube was inserted and purulent pleural fluid was drained.A. meyeriwas isolated in anaerobic cultures of the pleural fluid. The infection was alleviated in response to treatment with intravenous penicillin G (20 million IU daily) and oral amoxicillin (500 mg every 8 hours) for 4 months, demonstrating that short-term antibiotic treatment was effective.


2021 ◽  
Vol 9 (17) ◽  
pp. 6107-6117
Author(s):  
Xinyang Wang ◽  
Ling Zhao ◽  
Xinyong Li ◽  
Jincheng Mu ◽  
Liehao Wei ◽  
...  
Keyword(s):  

1979 ◽  
Vol 1 (5) ◽  
pp. 132-158

A (massive) multicenter study of 3,000 patients has demonstrated that skin tests to penicillin G and penicilloyl-polylysine (PPL-now commercially available) predict and confirm penicillin allergy. Of patients with a history of penicillin reaction, 19% were positive to either, compared to 7% of controls. A history of anaphylaxis led to 46% positive. Of those with a history of urticaria 17% were positive, and those with maculopapular eruptions did not differ from controls (7% positive). Challenge with penicillin led to a reaction in 6% with a positive history (compared to 2% with a negative) and 67% with a combined positive history and positive skin test (to either).


2021 ◽  
Author(s):  
Giovanni Baccolo ◽  
Barbara Delmonte ◽  
Paul Niles ◽  
Giannantonio Cibin ◽  
Elena Di Stefano ◽  
...  

&lt;p&gt;On Earth, jarosite is a weathering product forming in acidic-oxidative environments from the alteration of iron-bearing minerals in presence of liquid water. Typical settings where this iron-potassium hydrated sulphate is found, are weathering zones of pyrite-rich deposits, evaporative basins and fumaroles. Jarosite is not only known on Earth, it also occurs on Mars, where it was firstly identified by the Opportunity rover. The mineral was in fact recognized in the finely layered formations outcropping at Meridiani Planum and that were accurately investigated by the rover (Klingelh&amp;#246;fer et al. 2004). Since jarosite requires liquid water to form, its occurrence on Mars has been regarded as an evidence for the presence of liquid water in the geologic past of Mars (Elwood-Madden et al., 2004). Since then, many models have been proposed to describe the environments where the precipitation of Martian jarosite took place. The most accepted ones deal with evaporative basins similar to Earth&amp;#8217;s playas, others concern volcanic activity and hydrothermal processes. An alternative proposal predicted that jarosite may have formed as a consequence of weathering of mineral dust trapped in massive ice deposits, i.e. the ice-weathering model (Niles &amp; Michalsky, 2009). The hypothesis that jarosite formed on Mars because of low-temperature, acidic and water limited weathering, is not new (Burns, 1987), but until now no direct evidences were available to support it.&lt;/p&gt;&lt;p&gt;A potential Earth analogue to investigate such processes is deep Antarctic ice. We present a first investigation of deep ice samples from the Talos Dome ice core (East Antarctica) aimed at the identification of englacial jarosite, so as to support the ice-weathering model. Evidences gathered through independent techniques showed that jarosite is actually present in deep Antarctic ice and results from the weathering of dust trapped into ice. The process is controlled by the re-crystallization of ice grains and the concurrent re-location of impurities at grain-junctions, which both depend on ice depth. This study demonstrates that the deep englacial environment is suitable for jarosite precipitation. Our findings support the hypothesis that, as originally predicted by the ice-weathering model, paleo ice-related processes have been important in the geologic and geochemical history of Mars.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;References&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Burns, R. Ferric sulfates on Mars. &lt;em&gt;J. Geophys. Res.&lt;/em&gt; &lt;strong&gt;92&lt;/strong&gt;, E570-E574 (1987).&lt;/p&gt;&lt;p&gt;Elwood-Madden et al., 2004. Jarosite as an indicator of water-limited chemical weathering on Mars. &lt;em&gt;Nature&lt;/em&gt; &lt;strong&gt;431&lt;/strong&gt;, 821-823 (2004).&lt;/p&gt;&lt;p&gt;Klingelh&amp;#246;fer, G. et al. Jarosite and Hematite at Meridiani Planum from Opportunity's M&amp;#246;ssbauer Spectrometer. &lt;em&gt;Science&lt;/em&gt; &lt;strong&gt;306&lt;/strong&gt;, 1740-1745 (2004).&lt;/p&gt;&lt;p&gt;Niles, P. B. &amp; Michalski, J. M. Meridiani Planum sediments on Mars formed through weathering in massive ice deposits. &lt;em&gt;Nat. Geosci.&lt;/em&gt; &lt;strong&gt;2&lt;/strong&gt;, 215-220 (2009).&lt;/p&gt;


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