First Report of Natural Occurrence of Zucchini Lethal Chlorosis Tospovirus on Cucumber and Chrysanthemum Stem Necrosis Tospovirus on Tomato in Brazil

During a field survey in 1994, five cucumber (Cucumis sativus) cv. Hokushin plants showing symptom of yellowing, mottling, and vein banding on the leaves were collected from a commercial field of the Federal District. By electron microscopy, quasi-spherical particles with double membrane, typical tospovirus-like particles were found in the infected leaf material. All samples strongly reacted with antibody of zucchini lethal chlorosis tospovirus (ZLCV), but not with antibodies of other to-spoviruses reported in Brazil (1): tomato spotted wilt virus (TSWV), tomato chlorotic spot virus (TCSV), groundnut ringspot virus (GRSV), chrysanthemum stem necrosis virus (CSNV), or iris yellow spot virusonion isolate (IYSV-BR). The virus was identified as ZLCV, which was first isolated in 1994 from zucchini (Cucurbita pepo) in São Paulo State, Brazil. Tomato (Lycopersicon esculentum) plants showing stem necrosis and necrotic spots and rings on the leaves were collected in Viçosa, Minas Gerais State. By electron microscopy, molecular studies, and enzyme-linked immunosorbent assay with antibodies of the six tospoviruses occurring in Brazil, the virus was identified as CSNV. This virus was first reported in 1995 on a Chrysanthemum sp. in São Paulo State and recently reported in the Netherlands from Dendranthema indicum. This is the first report of the natural occurrence of ZLCV and CSNV on cucumber and tomato, respectively. Reference: (1) A. C. de Ávila et al. 1998. Pages 32–34 in: Int. Symp. on Tospoviruses and Thrips in Floral and Vegetable Crops, 4th.

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Root-Knot Disease Caused by Meloidogyne arenaria and M. javanica in Teak in São Paulo State, Brazil

Plant Disease ◽

10.1094/pdis-05-11-0442 ◽

2012 ◽

Vol 96 (1) ◽

pp. 151-151

Author(s):

J. V. de Araújo Filho ◽

A. C. Z. Machado ◽

R. S. C. A. de Faria ◽

L. E. A. Camargo

Keyword(s):

Sao Paulo ◽

The State ◽

Meloidogyne Arenaria ◽

São Paulo ◽

Mato Grosso ◽

Lateral Field ◽

First Report ◽

Paulo State ◽

São Paulo State ◽

Esterase Phenotypes

Teak (Tectona grandis Linn. F.) is one of the most important forest crops in Brazil, occupying areas in different regions, such as Goiás, Mato Grosso, Paraná, and São Paulo states. Teak wood is used for many purposes such as shipbuilding, rolling and plywood, firewood, and charcoal. In May 2011, teak symptomatic feeder root samples, exhibiting inconspicuous, small galls, were collected in the municipality of Piracicaba, São Paulo State, Brazil (22°41′46.90″S, 47°38′36.84″W). Specimens were identified through perineal patterns and esterase phenotypes of 20 adult females (1,2). Perineal patterns and esterase phenotypes were consistent with those described for Meloidogyne arenaria (Neal, 1889) Chitwood, 1949 and M. javanica (Treub, 1885) Chitwood, 1949. Perineal patterns of M. arenaria showed a low dorsal arch, compressed dorsolaterally, with lateral field marked by some forked and broken striae; no punctate markings between anus and tail terminus were observed. Perineal patterns of M. javanica were rounded, with low dorsal arch, striae smooth, lateral field distinct, clearly demarcated from striae by parallel lines. From the esterase electrophoresis we obtained A2 (Rm:1.2;1.3) and J3 (Rm:1.0;1.25;1.4) phenotypes, typical from M. arenaria and M. javanica, respectively. To our knowledge, this is the first report of M. arenaria parasitizing teak roots in Brazil and elsewhere (new host) and the first report of M. javanica infecting teak in the State of São Paulo. Previously, M. javanica was reported to be infecting teak-growing areas in the State of Mato Grosso (3). This finding has a great importance, not only by the inclusion of these parasites in teak pathological scenario, but also for predicting possible damage in plant species used in teak-based intercropping systems. References: (1) P. R. Esbenshade and A. C. Triantaphyllou. J. Nematol. 22:10, 1990. (2) K. M. Hartman and J. N. Sasser. 1985. Page 115 in: An Advanced Treatise on Meloidogyne. Volume II, Methodology. K. R. Barker et al., eds. North Carolina State University Graphics, Raleigh,1985. (3) R. A. Silva et al. Nematol. Bras. 27:261, 2003.

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Scanning Electron Microscopy (SEM) in the surficial cover analysis of the low fluvial-marine terraces in the southern coast of São Paulo state, Brazil

Revista Brasileira de Geomorfologia ◽

10.20502/rbg.v23i1.1959 ◽

2022 ◽

Vol 23 (1) ◽

Author(s):

André De Oliveira Souza ◽

Archimedes Perez Filho

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Sao Paulo ◽

São Paulo ◽

Southern Coast ◽

Paulo State ◽

Marine Terraces ◽

São Paulo State ◽

Scanning Electron

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Olpidium gregarium, a chytrid fungus affecting rotifers populations in Rio Grande Reservoir, São Paulo State, Brazil

Biota Neotropica ◽

10.1590/s1676-06032013000100036 ◽

2013 ◽

Vol 13 (1) ◽

pp. 356-359 ◽

Cited By ~ 1

Author(s):

Patrícia do Amaral Meirinho ◽

Paula Yuri Nishimura ◽

Carmen Lidia Amorim Pires-Zottarelli ◽

Viviane Mochini-Carlos ◽

Marcelo Luiz Martins Pompêo

Keyword(s):

Rio Grande ◽

Sao Paulo ◽

Central Axis ◽

São Paulo ◽

Chytrid Fungus ◽

First Report ◽

Paulo State ◽

São Paulo State ◽

Rio Grande Reservoir

A chytrid fungus Olpidium gregarium was found in Rio Grande Reservoir as rotifers populations' parasite. Along the reservoir central axis, the rotifers density decreased in the dam direction, while the density of affected rotifers by the chytrid fungus increased in the same direction. Moreover, some rotifers species were more affected than others, thus showing certain preference for some rotifers species. Thereby, this parasite may be interfering in the distribution of rotifers population in Rio Grande Reservoir. This is the first report of this species for Brazil.

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Mites associated with the eared dove, Zenaida auriculata (Des Murs, 1847), in São Paulo State, Brazil*

Zoosymposia ◽

10.11646/zoosymposia.6.1.36 ◽

2011 ◽

Vol 6 (1) ◽

pp. 267-274 ◽

Cited By ~ 4

Author(s):

THAÍS M. GOULART ◽

DAVI L. MORAES ◽

ANGELO P. PRADO

Keyword(s):

Mite Species ◽

Sao Paulo ◽

São Paulo ◽

First Report ◽

Paulo State ◽

São Paulo State ◽

Ornithonyssus Bursa

The aim of this study was to report the mite species found in association with the eared dove, Zenaida auriculata (Des Murs, 1847), in São Paulo State, Brazil. A total of 34 bird specimens was examined, and mites were found on 31 of them. The following numbers of mite species were found: Astigmata: Falculiferidae - three species; Analgidae - two species; Dermoglyphidae, Epidermoptidae, Hypoderatidae and Pyroglyphidae - one species each. Prostigmata: Cheyletidae, Cheyletiellidae and Syringophilidae - one species each. Mesostigmata: Macronyssidae and Rhinonyssidae - one species each. We present the first report of Z. auriculata as host of Diplaegidia columbae Buchholz, 1869; Diplaegidia columbigallinae Cerny´, 1975; Byersalges talpacoti Cerny´, 1975; Pterophagus spilosikyus Gaud & Barré, 1992; Hypodectes propus (Nitzch in Giebel, 1861), Ornithocheyletia columbigallinae Fain & Bochkov, 2002; Ornithonyssus bursa (Berlese, 1888) and Tinaminyssus zenaidurae (Crossley, 1952).

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Epidemiological and ecological aspects related to malaria in the area of influence of the lake at Porto Primavera dam, in western São Paulo State, Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652008000500008 ◽

2008 ◽

Vol 50 (5) ◽

pp. 287-295 ◽

Cited By ~ 12

Author(s):

Almério de C. Gomes ◽

Marcia B. de Paula ◽

Ana Maria R. de C. Duarte ◽

Maura A. Lima ◽

Rosely dos S. Malafronte ◽

...

Keyword(s):

Environmental Changes ◽

Sao Paulo ◽

Enzyme Linked Immunosorbent Assay ◽

Hydroelectric Power Station ◽

São Paulo ◽

Serological Tests ◽

Sylvatic Cycle ◽

Paulo State ◽

São Paulo State ◽

Area Of Influence

A study was carried out in the area of influence of the Porto Primavera Hydroelectric Power Station, in western São Paulo State, to investigate ecological and epidemiological aspects of malaria in the area and monitor the profile of the anopheline populations following the environmental changes brought about by the construction of the lake. Mosquitoes captured were analyzed by standardized indicator species analysis (ISA) before and during different flooding phases (253 m and 257 m elevations). The local human population was studied by means of parasitological (thin/thick blood smears), molecular (PCR) and serological tests. Serological tests consisted of Enzyme Linked Immunosorbent Assay (ELISA) with synthetic peptides of the circumsporozoite protein (CSP) from classic Plasmodium vivax, P. vivax variants (VK247 and "vivax-like"), P. malariae and P. falciparum and Indirect Immunofluorescence Assay (IFA) with asexual forms of P. vivax, P. malariae and P. falciparum. The results of the entomological survey indicated that, although the Anopheles darlingi population increased after the flooding, the population density remained very low. No malaria, parasite infection or DNA was detected in the inhabitants of the study area. However, there was a low frequency of antibodies against asexual forms and a significant prevalence of antibodies against P. vivax, P. vivax variants, P. falciparum and P. malariae; the presence of these antibodies may result from recent or less recent contact with human or simian Plasmodium (a parallel study in the same area revealed the existence of a sylvatic cycle). Nevertheless, these results suggest that, as in other places where malaria is present and potential vectors circulate, the local epidemiological conditions observed could potentially support the transmission of malaria in Porto Primavera Lake if infected individuals are introduced in sufficient numbers. Further studies are required to elucidate the phenomena described in this paper.

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Visceral larva migrans: a seroepidemiological survey in five municipalities of São Paulo State, Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651990000300010 ◽

1990 ◽

Vol 32 (3) ◽

pp. 204-210 ◽

Cited By ~ 44

Author(s):

Pedro Paulo Chieffi ◽

Mirthes Ueda ◽

Eide Dias Camargo ◽

Ana Maria Carvalho de Souza ◽

Marilda L. da Silva Guedes ◽

...

Keyword(s):

Toxocara Canis ◽

Sao Paulo ◽

Larva Migrans ◽

Enzyme Linked Immunosorbent Assay ◽

São Paulo ◽

Paulo State ◽

São Paulo State ◽

The Difference ◽

Male Subjects ◽

Positive Results

An enzyme-linked immunosorbent assay (ELISA), employing antigens from Toxocara canis larvae and the absortion of suspected sera with Ascaris lumbricoides extracts was used in a seroepidemiological study performed in five municipalities of São Paulo State, Brazil (São Paulo, Campinas, Santos, Marília and Presidente Prudente) in order to determine the frequency of antibodies to Toxocara. In 2,025 blood samples collected, 806 proceeded from male subjects and 1,219 from females; 483 samples were collected from subjects under 15 years of age and the remaining 1,542 from subjects aged 15 years or over. Among the 2,025 sera investigated, 3.60% had antibodies to Toxocara at significant levels. A moderate predominance of infection with Toxocara among male subjects (3.72%) was observed, although the difference was not statistically significant when this rate was compared with that for female (3.28%). Related to age, a higher frequency of positive results was detected among subjects under 15 years (6.41%) against the older group (2.53%). A trend of more elevated rates of infection was observed in municipalities with high demographic densities (São Paulo, Campinas and Santos). Nevertheless, such findings only appeared to be statistically significant in subjects younger than 15 years.

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