scholarly journals First Report of a Disease of Peony Caused by Alfalfa mosaic virus

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 99-99 ◽  
Author(s):  
M. G. Bellardi ◽  
C. Rubies-Autonell ◽  
A. Bianchi
Keyword(s):  
Mosaic Virus ◽  
Leaf Roll ◽  
Tobacco Rattle Virus ◽  
Alfalfa Mosaic Virus ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Systemic Leaf ◽  
Cherry Leaf Roll Virus ◽  
Line Patterns

During the summers of 2001 and 2002, Japanese peony (Paeonia albiflora Pall., synonym P. lactiflora, family Paeoniaceae) plants, cultivated in the Botanical Garden of the University of Parma (Emilia Romagna Region of northern Italy), were found affected by a disease with virus-like symptoms. The oldest leaves showed yellow, mosaic, oak-like arabesques and line-patterns; the remaining leaves and pink flowers were symptomless. A disease of peony, known as peony ring spot disease, has been reported worldwide (Europe, United States, Japan, and New Zeland) for several years and is associated with strains of Tobacco rattle virus (TRV) (1). Electron microscopic observations of peony leaf sap (leaf dip preparations stained with uranyl acetate and phospotungstic acid) did not show the presence of any rod-shaped virus particles, including TRV. Mechanical inoculations of sap from symptomatic leaves caused symptoms typical of Alfalfa mosaic virus (AMV) on Chenopodium amaranticolor Coste & Reyn. (local chlorotic and necrotic lesions and systemic periveinal line-patterns), Ocimum basilicum L. (yellow mosaic), Vigna unguiculata (L.) Walp. (red, local necrotic lesions), and Nicotiana tabacum cv. Samsun (white, necrotic lesions, systemic leaf malformation, and mosaic), and N. glutinosa L. (systemic leaf variegation). Symptomatic leaves of peony and infected herbaceous plants were analyzed for virus presence by protein A sandwich enzyme-linked immunosorbent assay (PAS-ELISA). The polyclonal antisera tested were those to AMV (PVAS 92, American Type Culture Collection, Manassas, VA), AMV-Vinca minor L. (DiSTA collection, Italy), and the nepoviruses Strawberry latent ringspot virus, Tomato ringspot virus, and Cherry leaf roll virus. PAS-ELISA revealed only the presence of AMV. Immunoelectron microscopy and gold-labeled decoration confirmed the identity of the virus. In 2001, five symptomless peony plants (provided by a commercial grower and previously analyzed for AMV and TRV presence) were grafted with shoots from peony showing yellow mosaic on the leaves and maintained in a greenhouse under aphid-proof cage. During the summer of 2002, one of the grafted plants showed a mild mosaic on the leaves; PAS-ELISA revealed this peony was infected by AMV. To our knowledge, this is the first report of AMV in peony. Reference: (1) Chang et al. Ann. Phytopathol. Soc. Jpn. 42:325, 1976.

Identification of Two Tobacco rattle virus Sequence Variants Associated with Virus-like Mottle Symptom on Hosta in Ohio

2013 ◽  
Vol 14 (1) ◽  
pp. 24 ◽  
Author(s):  
John R. Fisher
Keyword(s):  
Mosaic Virus ◽  
Tobacco Rattle Virus ◽  
Alfalfa Mosaic Virus ◽  
Pcr Primers ◽  
Ringspot Virus ◽  
Impatiens Necrotic Spot Virus ◽  
Sequence Variants ◽  
Arabis Mosaic Virus ◽  
Specific Pcr ◽  
Wilt Virus

Two Hosta sp. ‘So Sweet’ plants and one Hosta sieboldii (labeled as ‘Albo-marginata’) plant showing a suspected virus-like leaf mottle symptom tested negative for the Potyvirus group, Hosta virus X, Alfalfa mosaic virus, Arabis mosaic virus, Cucumber mosaic virus, Impatiens necrotic spot virus, Tobacco mosaic virus, Tobacco ringspot virus, Tomato ringspot virus, and Tomato spotted wilt virus by ELISA. DsRNA analysis produced a banding profile suggestive of a viral infection, and dsRNA was used as template to synthesize cDNAs for use with tobravirus group and Tobacco rattle virus (TRV) specific PCR primers. Amplicons were cloned and sequenced, and results showed two distinct populations of sequences: the two So Sweet isolates were ∼99% identical to each other but only ∼92% identical to the Albo-marginata isolate. These results represent the first confirmed report of TRV in Hosta in Ohio, and further demonstrate that there are at least two nucleotide sequence variants of the virus infecting Ohio Hosta. Accepted for publication 21 December 2012. Published 30 March 2013.

scholarly journals First Report of Alfalfa mosaic virus in Lavandula officinalis

Plant Disease ◽  
2004 ◽  
Vol 88 (8) ◽  
pp. 908-908 ◽  
Author(s):  
Ll. Martínez-Priego ◽  
M. C. Córdoba ◽  
C. Jordá
Keyword(s):  
Mosaic Virus ◽  
Screening Program ◽  
Sequence Data ◽  
Alfalfa Mosaic Virus ◽  
Mediterranean Area ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Coat Protein Region ◽  
C 30 ◽  
Lavandula Officinalis

For several years, in ornamental nurseries in the Mediterranean area of Spain, stunting and yellow leaf spotting have been observed in young plants of Lavandula officinalis. Symptoms eventually disappeared as the plants matured. During the summer of 2003, the number of plantlets affected and the intensity of symptoms increased significantly. Symptomatic plants tested positive using enzyme-linked immunosorbent assay (ELISA) (Phyto-Diagnostics, INRA, France) for the presence of Alfalfa mosaic virus (AMV). ELISA results were verified using reverse transcription-polymerase chain reaction (RT-PCR). Total RNA extracts from symptomatic plants were analyzed using primers designed specifically for the coat protein region of AMV utilizing sequence data from GenBank Accession No. AF215664: AMVcoat-F: GT GGT GGG AAA GCT GGT AAA and AMVcoat-R: CAC CCA GTG GAG GTC AGC ATT. The thermocycling schedule was as follows: reverse transcriptase step at 50°C for 30 min, first PCR cycle at 94°C for 2 min, 35 cycles each of 30 s at 94°C, 30 s at 54°C, 30 s at 72°C, followed by a final extension at 72°C for 10 min. A 700-pb PCR product of the expected size was obtained from plants that were positive for AMV using ELISA. The two systems provide for rapid detection of AMV in L. officinalis. A regular screening program will assist in providing virus-free plants to ornamental nurseries. These results demonstrate the presence of AMV in L. officinalis. Alfalfa (Medicago sativa L.) is a typical source of AMV. However, because the nurseries where L. officinalis is grown are not in the vicinity of alfalfa fields, we suggest the source of the infection originated in the propagation material. AMV has currently been reported in L. officinalis only in Italy and France (1). To our knowledge, this is the first report of AMV in L. officinalis in Spain. Reference: (1): A. Garibaldi et al. Ed. Edagricole-Edisioni Agricole della Calderini s.r.l., Bologna, 2000.

scholarly journals Macroarray Detection of Eleven Potato-Infecting Viruses and Potato spindle tuber viroid

Plant Disease ◽  
2008 ◽  
Vol 92 (5) ◽  
pp. 730-740 ◽  
Author(s):  
Bright Agindotan ◽  
Keith L. Perry
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Tobacco Rattle Virus ◽  
Alfalfa Mosaic Virus ◽  
Potato Virus X ◽  
Potato Spindle Tuber Viroid ◽  
Apparent Competition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Seed ◽  
Potato Virus S

A macroarray was developed for the detection of 11 potato viruses and Potato spindle tuber viroid. The 11 viruses detected included those commonly found or tested for in North American potato seed certification programs: Alfalfa mosaic virus, Cucumber mosaic virus, Potato mop top virus, Potato leafroll virus, Potato latent virus, Potato virus A, Potato virus M, Potato virus S, Potato virus X, Potato virus Y, and Tobacco rattle virus. These viruses were detected using oligonucleotide 70-mer probes and labeled targets prepared by a random primed amplification procedure. Potato plants analyzed included those infected with 12 reference virus stocks and 36 field isolates. Results from the macroarray were entirely consistent with those obtained using a standard serological assay (enzyme-linked immunosorbent assay). Four isolates of Potato spindle tuber viroid, in mixed infection with one or more viruses, also were detected in the array, although strong hybridization signals required amplification with viroid-specific primers in combination with anchored-random primers. In individual plants, up to four viruses, or a viroid plus two viruses, were detected, with no apparent competition or inhibition. Macroarrays are a cost-effective approach to the simultaneous diagnostic detection of multiple pathogens from infected plants.

scholarly journals Occurrence and Relative Incidence of Viruses Infecting Soybeans in Iran

Plant Disease ◽  
2004 ◽  
Vol 88 (10) ◽  
pp. 1069-1074 ◽  
Author(s):  
A. R. Golnaraghi ◽  
N. Shahraeen ◽  
R. Pourrahim ◽  
Sh. Farzadfar ◽  
A. Ghasemi
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Soybean Seed ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Tobacco Streak Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Natural Occurrence

A survey was conducted to determine the incidence of Alfalfa mosaic virus (AlMV), Bean common mosaic virus (BCMV), Bean yellow mosaic virus (BYMV), Blackeye cowpea mosaic virus (BlCMV), Cucumber mosaic virus (CMV), Pea enation mosaic virus (PEMV), Peanut mottle virus (PeMoV), Soybean mosaic virus (SMV), Tobacco mosaic virus (TMV), Tobacco ringspot virus (TRSV), Tobacco streak virus (TSV), Tomato ringspot virus (ToRSV), and Tomato spotted wilt virus (TSWV) on soybean (Glycine max) in Iran. Totals of 3,110 random and 1,225 symptomatic leaf samples were collected during the summers of 1999 and 2000 in five provinces of Iran, where commercial soybean is grown, and tested by enzyme-linked immunosorbent assay (ELISA) using specific polyclonal antibodies. Serological diagnoses were confirmed by electron microscopy and host range studies. The highest virus incidence among the surveyed provinces was recorded in Mazandaran (18.6%), followed by Golestan (15.7%), Khuzestan (14.2%), Ardabil (13.9%), and Lorestan (13.5%). Incidence of viruses in decreasing order was SMV (13.3%), TSWV (5.4%), TRSV (4.2%), TSV (4.1%), PEMV (2.9%), BYMV (2.2%), ToRSV (2.1%), AlMV (1.3%), BCMV (0.8%), and CMV (0.6%). Additionally, 1.5% of collected leaf samples had positive reactions in ELISA with antiserum to TMV, indicating the possible infection of soybeans in Iran with a Tobamovirus that is related serologically to TMV. Of 195 leaves from plants showing soybean pod set failure syndrome (PSF) in Mazandaran and Lorestan, only 14 (7.2%) samples had viral infection. No correlation was observed between PSF and presence of the 13 viruses tested, suggesting the involvement of other viruses or factors in this syndrome. To investigate the presence of seed-borne viruses, including SMV, TRSV, ToRSV, and TSV, 7,830 soybean seeds were collected randomly at harvesting time from the major sites of soybean seed production located in Mazandaran and Golestan provinces. According to ELISA analyses of germinated seedlings, 7.1 and 8.9% of the seed samples from Golestan and Mazandaran provinces, respectively, transmitted either SMV, TRSV, ToRSV, or TSV through seed. We also showed that SMV and other seed transmissible viruses, as well as TSWV, usually are the most prevalent viruses in soybean fields in Iran. In this survey, natural occurrence of AlMV, BCMV, BlCMV, BYMV, CMV, PEMV, PeMoV, and TSWV was reported for the first time on soybeans in Iran.

scholarly journals First Report of Papaya ringspot virus W in Sponge Gourd from India

Plant Disease ◽  
2006 ◽  
Vol 90 (7) ◽  
pp. 974-974 ◽  
Author(s):  
Raj Verma ◽  
V. K. Baranwal ◽  
Satya Prakash ◽  
S. P. S. Tomer ◽  
R. P. Pant ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Natural Occurrence ◽  
Papaya Ringspot Virus ◽  
First Report ◽  
Sponge Gourd ◽  
Das Elisa

During August 2004, symptoms resembling a virus disease were observed in commercial cultivation of sponge gourd (Luffa cylindrica (L.) M. Roem. [cv. Chikni]) in Pune, India. Affected plants exhibited mosaic mottling, vein banding, and downward marginal curling on leaves. The incidence of disease was 10 to 30% based on the symptoms and confirmed using enzyme-linked immunosorbent assay (ELISA). Six fields of sponge gourd were visited, and 30 samples were collected randomly. Samples from affected fields were analyzed for the presence of virus by mechanical inoculation. Symptoms typical of those observed in the original field plants appeared 10 days after inoculation on sponge gourd. The virus isolate produced local lesions on Chenopodium amaranticolor and systemic symptoms on Cucumis melo, Cucumis sativus, Cucurbita maxima, Cucurbita pepo, L. acutangula, and L. cylindrica, but did not produce symptoms on Carica papaya (cvs. CO2 and Red Lady), Nicotiana glutinosa, N. tabacum (cv. White Burley), or Vigna unguiculata. Field-infected sponge gourd and all indicator plants were tested using double-antibody sandwich (DAS)-ELISA. The virus was identified as Papaya ringspot virus-W (PRSV-W) using DAS-ELISA (Agdia, Elkhart, IN). A strong, positive reaction was obtained with antiserum to PRSV-W but not with antisera to PRSV-P, Cucumber mosaic virus, Squash mosaic virus, Zucchini yellow mosaic virus, and Groundnut bud necrosis virus. To ensure virus purity, the isolate was passed through three successive single-lesion transfers on C. amaranticolor. Flexuous filamentous particles 775 nm long were observed with electron microscopy of leaf-dip preparation from symptomatic sponge gourd leaves. The virus particles were then decorated with a second PRSV-W antiserum obtained from the Plant Virology Unit, IARI, New Delhi. PRSV-W on sponge gourd has previously been reported from Taiwan (1). To our knowledge, this is the first report of natural occurrence of PRSV-W on sponge gourd in India. Reference: (1.) C. H. Huang et al. J. Agri. Res. China 36:413, 1987.

scholarly journals Detection and phylogeny of viruses in native Albanian olive varieties

2021 ◽  
Vol 60 (1) ◽  
pp. 165-174
Author(s):  
Toufic ELBEAINO ◽  
Magdalena CARA ◽  
Shpend SHAHINI ◽  
Pasko PANDELI
Keyword(s):  
Mosaic Virus ◽  
Leaf Roll ◽  
Olive Tree ◽  
Ringspot Virus ◽  
Arabis Mosaic Virus ◽  
Cherry Leaf Roll Virus ◽  
Leaf Yellowing ◽  
Standard Quality ◽  
Olive Trees ◽  
Olive Varieties

Forty samples representing 14 native Albanian and two foreign olive varieties were collected from an olive varietal collection plot in the Valias region (Tirana, Albania). The samples were assayed by RT-PCR for presence of olive-infecting viruses, including arabis mosaic virus (ArMV), cherry leaf roll virus (CLRV), cucumber mosaic virus (CMV), olive latent ringspot virus (OLRSV), olive latent virus 1 (OLV-1), olive leaf yellowing-associated virus (OLYaV), strawberry latent ringspot virus (SLRSV) and by PCR for the bacterium Xylella fastidiosa (Xf). Ninety-eight percent of the samples were infected with at least one virus. OLYaV was the most prevalent (85% of samples), followed by OLV-1 (50%), OLRSV (48%), CMV (28%), SLRSV (3%) and CLRV (5%), whereas ArMV and Xf were absent. Fifty-five percent of the samples were infected with one virus, 13% with two viruses, 20% with three, and 5% with four. Analyses of the nucleotide sequences of the Albanian virus isolates generally showed low genetic variability, and that most were phylogenetically related to Mediterranean isolates, in particular to those from Greece and Italy. Five olive trees, representing three native cultivars (‘Managiel’, ‘Kalinjot’ and ‘Kushan-Preze’) and one foreign (‘Leccino’), were found to be plants of the Conformitas Agraria Communitatis (“CAC”) category i.e. free of ArMV, CLRV, SLRSV and OLYaV. Only one tree of the native cultivar ‘Ulliri i kuq’ was free of all tested viruses, so this is plant material of the “Virus-tested” category. Olives derived from both categories could be used for propagation of standard quality plant materiel in a future certification programme for olive in Albania. This is the first report of CLRV, OLRSV, CMV and OLV-1 in Albania. The study also reveals the precarious health status of native olive varieties in the Valias varietal collection plot. However, the discovery of six plants representing two certifiable categories is a first step in a future olive tree certification program in the country.

scholarly journals First Report of Rose yellow vein virus in Rosa sp. in New Zealand

Plant Disease ◽  
2013 ◽  
Vol 97 (8) ◽  
pp. 1122-1122 ◽  
Author(s):  
Z. Perez-Egusquiza ◽  
L. W. Liefting ◽  
L. I. Ward
Keyword(s):  
New Zealand ◽  
Mosaic Virus ◽  
The United States ◽  
Ringspot Virus ◽  
Yellow Vein ◽  
Yellow Mosaic Virus ◽  
First Report ◽  
Chlorotic Mottle ◽  
Cherry Leaf Roll Virus ◽  
Two Samples

Rose is the top selling cut flower in New Zealand and is the most popular garden plant in the world. Several virus-like diseases have been described in roses, but the causal agents for many remain unknown. Most of the described viruses infecting rose belong to the genera Ilarvirus and Nepovirus. Only recently, a number of new viruses have been or are in the process of being characterized (1,2,3,4). In January 2011, 10 rose samples showing virus-like symptoms were collected from the Wanganui region on the North Island of New Zealand. Total nucleic acid was extracted from these samples using an InviMag Plant DNA Mini Kit (Invitek GmbH, Berlin, Germany) and a KingFisher mL workstation (Thermo Scientific, Waltham, MA). PCR and reverse transcription (RT)-PCR was conducted using specific primers for Arabis mosaic virus (ArMV), Cherry leaf roll virus, Prunus necrotic ringspot virus (PNRSV), Rosa rugosa leaf distortion virus, Rose spring dwarf associated virus, Rose yellow leaf virus, Rose yellow mosaic virus, Rose yellow vein virus (RYVV), and Strawberry latent ringspot virus. Samples were also tested using generic primers for carlavirus, potexvirus, potyvirus, tombusvirus, and phytoplasmas. Two samples (cvs. Pauls Himalayan Musk and Bloomfield) were positive for ArMV, four samples (cvs. Leda, Rosa Mundi, Charles de Mills, and Indica Major) were positive for PNRSV, and two samples (cvs. Leda and Zephirine Drouhin) were positive for RYVV. Samples were negative for all other tested viruses and phytoplasmas. RYVV was detected using two sets of primers (D. Mollov, personal communication) designed to amplify fragments of estimated sizes of 797 bp and 684 bp of the movement protein (MP) and coat protein (CP) genes of RYVV, respectively. RYVV amplicons were sequenced directly (GenBank Accession Nos. JX887423 to JX887426). A BLASTn search of the MP and CP fragments showed the highest nucleotide identity of 98% and 96 to 97%, respectively, with the type isolate of RYVV (JX028536). RYVV has been reported as the causal agent of a vein yellowing disease in rose (2). Symptoms observed in the ‘Leda’ sample infected with PNRSV and RYVV (vein yellowing and chlorotic mottle in the apex of leaves) were not typical of PNRSV, so they may be caused by RYVV. Symptoms in samples of cv. Zephirine Drouhin (curling of leaves and mottle), observed in both RYVV-positive and -negative samples, may not be associated with RYVV infection. This suggests that vein yellowing may be influenced by cultivar. RYVV has been reported in several rose cultivars, but only in the United States (2). To the best of our knowledge, this is the first report of RYVV infecting rose in New Zealand, where it is likely that the virus has been present for some time. The virus may have a much wider geographical distribution than that reported as the virus was only recently characterized (3). References: (1) B. Lockhart et al. Page 31 in: Program and Abstracts of The 12th International Symposium on Virus Diseases of Ornamental Plants, 2008. (2) D. Mollov et al. Phytopathology 99:S87, 2009. (3) D. Mollov et al. Arch Virol. 158:877, 2012. (4) N. Salem et al. Plant Dis. 92:508, 2008.

scholarly journals Identification, Characterization, and Molecular Detection of Alfalfa mosaic virus in Potato

2006 ◽  
Vol 96 (11) ◽  
pp. 1237-1242 ◽  
Author(s):  
H. Xu ◽  
J. Nie
Keyword(s):  
Mosaic Virus ◽  
Gene Sequence ◽  
Alfalfa Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Polymorphism Analysis ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Simple Method ◽  
Cp Gene ◽  
Nucleotide Sequence Alignment

Alfalfa mosaic virus (AMV) was detected in potato fields in several provinces in Canada and characterized by bioassay, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction (RT-PCR). The identity of eight Canadian potato AMV isolates was confirmed by sequence analysis of their coat protein (CP) gene. Sequence and phylogenetic analysis indicated that these eight AMV potato isolates fell into one strain group, whereas a slight difference between Ca175 and the other Canadian AMV isolates was revealed. The Canadian AMV isolates, except Ca175, clustered together among other strains based on alignment of the CP gene sequence. To detect the virus, a pair of primers, AMV-F and AMV-R, specific to the AMV CP gene, was designed based on the nucleotide sequence alignment of known AMV strains. Evaluations showed that RT-PCR using this primer set was specific and sensitive for detecting AMV in potato leaf and tuber samples. AMV RNAs were easily detected in composite samples of 400 to 800 potato leaves or 200 to 400 tubers. Restriction analysis of PCR amplicons with SacI was a simple method for the confirmation of PCR tests. Thus, RT-PCR followed by restriction fragment length polymorphism analysis may be a useful approach for screening potato samples on a large scale for the presence of AMV.

scholarly journals First report of the infection of alfalfa mosaic virus in Salvia sclarea in Hungary

2018 ◽  
Vol 100 (3) ◽  
pp. 607-607 ◽  
Author(s):  
Pal Salamon ◽  
Anita Sos-Hegedus ◽  
Peter Gyula ◽  
Gyorgy Szittya
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
First Report ◽  
Salvia Sclarea

scholarly journals First Report of Cucumber mosaic virus in Desert Rose in Taiwan

Plant Disease ◽  
2012 ◽  
Vol 96 (4) ◽  
pp. 593-593 ◽  
Author(s):  
Y. K. Chen ◽  
Y. S. Chang ◽  
Y. W. Lin ◽  
M. Y. Wu
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Chenopodium Quinoa ◽  
Rabbit Antiserum ◽  
Serological Tests ◽  
Reading Frame ◽  
First Report ◽  
Reverse Transcription Pcr ◽  
Wilt Virus ◽  
Line Patterns

Desert rose (Adenium obesum (Forssk.) Roem. & Schult, family Apocynaceae) is native to southeastern Africa, and is a perennial potted ornamental with colorful flowers that are popular in Taiwan. Symptoms of mosaic and chlorotic ringspots and line patterns on leaves were observed in July 2010, on all eight plants in a private garden in Potzu, Chiayi, Taiwan. Spherical virus particles with a diameter of approximately 28 nm were observed in crude sap prepared from symptomatic leaves. Virus culture was established by successive local lesion isolation in Chenopodium quinoa and was maintained in the systemic host Nicotiana tabacum van Hicks. The virus was mechanically transmissible to indicator plants and induced symptoms similar to those incited by Cucumber mosaic virus (CMV). Observed symptoms included local lesions on inoculated leaves of C. amaranticolor and systemic mosaic in Cucumis sativus, Lycopersicon esculentum, N. benthamiana, N. glutinosa, and N. rustica. On N. tabacum, necrotic ringspots developed on inoculated leaves followed by systemic mosaic. Serological tests using ELISA assays and western blotting indicated that the virus reacted positively to a rabbit antiserum prepared to CMV (4). Amplicons of an expected size (1.1 kb) were obtained in reverse transcription-PCR with primers specific to the 3′-half of CMV RNA 3 (3) using total RNA extracted from infected desert rose and N. tabacum. The amplified cDNA fragment was cloned and sequenced (GenBank Accession No. AB667971). Nucleotide sequences of the coat protein open reading frame (CP ORF) (657 nt) had 92 to 96% and 76 to 77% sequence identity to those of CMV in subgroups I (GenBank Accession Nos. NC_001440, D00385, M57602, D28780, and AB008777) and II (GenBank Accession Nos. L15336, AF127976, AF198103, and M21464), respectively. Desert roses infected by Tomato spotted wilt virus (TSWV) (1) and CMV (2) have been reported previously. In spite of the plants showing mosaic symptoms similar to that caused by CMV (2) and chlorotic ringspots and line patterns caused by TSWV (1), only CMV was detected in and isolated from these infected desert roses. However, the possibility of mixed infection of CMV and other viruses were not excluded in this research. To our knowledge, this is the first report of CMV infection in desert rose plants occurring in Taiwan. References: (1) S. Adkins and C. A. Baker. Plant Dis. 89:526, 2005. (2) C. A. Baker et al. Plant Dis. 87:1007, 2003. (3) Y. K. Chen et al. Arch. Virol. 146:1631, 2001. (4) Y. K. Chen and C. C. Yang. Plant Dis. 89:529, 2005.

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