scholarly journals Distribution of Pathotypes of Rhynchosporium secalis and Cultivar Reaction on Barley in Alberta

Plant Disease ◽  
2003 ◽  
Vol 87 (4) ◽  
pp. 391-396 ◽  
Author(s):  
K. Xi ◽  
T. K. Turkington ◽  
J. H. Helm ◽  
K. G. Briggs ◽  
J. P. Tewari ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Environmental Conditions ◽  
Rhynchosporium Secalis ◽  
Pathogen Virulence ◽  
Commercial Cultivars ◽  
Major Resistance Genes ◽  
Different Levels

Forty-four barley accessions and commercial cultivars with different levels of resistance to scald caused by Rhynchosporium secalis were evaluated for scald reaction from 1997 to 1999 at various sites in Alberta. The accessions Hudson, Atlas, Atlas 46, Atlas 68, Abyssinian, and Kitchin that have the major resistance genes were resistant to pathotypes of R. secalis at all sites. Although scald levels were low for these accessions, they were significantly different among years. Pathotypes of R. secalis and environmental conditions affected diseases levels on 32 commercial cultivars, resulting in significantly different scald reactions among sites and seasons. Resistance in commercial cultivars, AC Stacy, Kasota, and Seebe, held up at most sites with the majority of cultivars being intermediate to moderately susceptible. Cultivars that were previously considered resistant were intermediate in reaction and became increasingly susceptible at some sites from 1997 to 1999. Pathogen virulence was more diverse at the sites where the cultivars became increasingly susceptible compared with sites where the same cultivars were resistant. Scald reactions of the commercial cultivars depended on location, which reflected the presence of different pathotypes, as well as variation in environmental conditions. Consequently, scald management via cultivar choice will be dependent on location.

Hierarchical structure of pathogenic variation among Rhynchosporium secalis populations in Idaho and Oregon

1992 ◽  
Vol 70 (4) ◽  
pp. 810-817 ◽  
Author(s):  
Stephen B. Goodwin ◽  
Robert W. Allard ◽  
Shirlee A. Hardy ◽  
Robert K. Webster
Keyword(s):  
Pacific Northwest ◽  
Resistance Genes ◽  
Local Population ◽  
Rhynchosporium Secalis ◽  
Causal Organism ◽  
Pathogenic Variation ◽  
Differential Cultivars ◽  
Electrophoretic Data ◽  
Differential Cultivar ◽  
Major Resistance Genes

Ninety-four isolates of Rhynchosporium secalis, the causal organism of the barley scald disease, from four populations (infected fields) in Idaho and eight in Oregon, were tested for pathogenicity to 14 barley differential cultivars; 60 distinct pathotypes, 33 in Idaho and 28 in Oregon, were found among the 94 isolates. Pathogenicity to most of the differentials was found in each local population sampled. One differential cultivar, CI 5831, was resistant to all isolates tested. Isolates from the same cultivar in different locations usually had different pathogenicities, and most pathotypes were genetically heterogeneous mixtures of many different genotypes based on previous electrophoretic data. The sampled cultivars generally had few or no resistance genes, suggesting that the R. secalis populations in Idaho and Oregon are characterized by large amounts of seemingly unnecessary pathogenicity. When the total pathogenic diversity was partitioned into hierarchical components using the Shannon information statistic, 58% was accounted for by the within-population component, 33% among populations within regions, and only 9% by the between-region component. Because most of the pathogenic variation is within local populations, these populations should adapt rapidly to introduced resistance genes; therefore traditional methods of breeding for resistance to R. secalis using single major resistance genes are not likely to be effective in the Pacific Northwest. Key words: pathogenicity index.

scholarly journals Identification and mapping of new genes for resistance to downy mildew in lettuce

2020 ◽  
Author(s):  
Lorena Parra ◽  
Kazuko Nortman ◽  
Anil Sah ◽  
Maria Jose Truco ◽  
Oswaldo Ochoa ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Small Chromosome ◽  
Pathogen Virulence ◽  
New Genes ◽  
Precise Knowledge ◽  
Low Pass ◽  
Major Resistance Genes ◽  
Chromosome Segments ◽  
Multiple Resistance Genes

Abstract Key message Eleven new major resistance genes for lettuce downy mildew were introgressed from wild Lactuca species and mapped to small regions in the lettuce genome. Abstract Downy mildew, caused by the oomycete pathogen Bremia lactucae Regel, is the most important disease of lettuce (Lactuca sativa L.). The most effective method to control this disease is by using resistant cultivars expressing dominant resistance genes (Dm genes). In order to counter changes in pathogen virulence, multiple resistance genes have been introgressed from wild species by repeated backcrosses to cultivated lettuce, resulting in numerous near-isogenic lines (NILs) only differing for small chromosome regions that are associated with resistance. Low-pass, whole genome sequencing of 11 NILs was used to identify the chromosome segments introgressed from the wild donor species. This located the candidate chromosomal positions for resistance genes as well as additional segments. F2 segregating populations derived from these NILs were used to genetically map the resistance genes to one or two loci in the lettuce reference genome. Precise knowledge of the location of new Dm genes provides the foundation for marker-assisted selection to breed cultivars with multiple genes for resistance to downy mildew.

scholarly journals Development of Bacterial Spot on Near-Isogenic Lines of Bell Pepper Carrying Gene Pyramids Composed of Defeated Major Resistance Genes

1999 ◽  
Vol 89 (11) ◽  
pp. 1066-1072 ◽  
Author(s):  
C. S. Kousik ◽  
D. F. Ritchie
Keyword(s):  
Disease Severity ◽  
Resistance Genes ◽  
Field Studies ◽  
Residual Effects ◽  
Specific Gene ◽  
Near Isogenic Lines ◽  
Bacterial Spot ◽  
Isogenic Lines ◽  
Major Resistance Genes ◽  
Race 4

Disease severity caused by races 1 through 6 of Xanthomonas campestris pv. vesicatoria on eight near-isogenic lines (isolines) of Early Calwonder (ECW) with three major resistance genes (Bs1, Bs2, and Bs3) in different combinations was evaluated in the greenhouse and field. Strains representing races 1, 3, 4, and 6 caused similar high levels of disease severity, followed by races 2 and 5 on susceptible ECW. Race 3 caused severe disease on all isolines lacking resistance gene Bs2. Race 4, which defeats Bs1 and Bs2, caused less disease on isoline ECW-12R (carries Bs1 + Bs2), than on isolines ECW, ECW-10R (carries Bs1), and ECW-20R (carries Bs2). Similar results were obtained with race 4 strains in field studies conducted during 1997 and 1998. In greenhouse studies, race 6, which defeats all three major genes, caused less disease on isoline ECW-13R (carries Bs1 + Bs3) and ECW-123R (carries Bs1 + Bs2 + Bs3) than on isolines ECW, ECW-10R, ECW-20R, and ECW-30R (carries Bs3), but not on ECW-23R (carries Bs2 + Bs3). In greenhouse studies with commercial hybrids, strains of races 4 and 6 caused less disease on Boynton Bell (carries Bs1 + Bs2) than on Camelot (carries no known resistance genes), King Arthur (carries Bs1), and X3R Camelot (carries Bs2). Race 6 caused less disease on hybrid R6015 (carries Bs1 + Bs2 + Bs3) and Sentinel (carries Bs1 + Bs3) than on Camelot. Residual effects were not as evident in field studies with race 6 strains. Defeated major resistance genes deployed in specific gene combinations (i.e., gene pyramids) were associated with less area under the disease progress curve than when genes were deployed individually in isolines of ECW or commercial hybrids. Successful management of bacterial spot of pepper is achieved incrementally by integrating multiple tactics. Although there is evidence of residual effects from defeated genes, these effects alone likely will not provide acceptable bacterial spot control in commercial production fields. However, when combined with sanitation practices and a judicious spray program, pyramids of defeated resistance genes may aid in reducing the risk of major losses due to bacterial spot.

Host range and physiologic specialization in Rhynchosporium secalis

1974 ◽  
Vol 25 (1) ◽  
pp. 21 ◽  
Author(s):  
SM Ali ◽  
WJR Boyd
Keyword(s):  
Genetic Diversity ◽  
Host Range ◽  
Environmental Conditions ◽  
Host Specialization ◽  
Rhynchosporium Secalis ◽  
Host Reaction ◽  
Genetic Studies ◽  
Barley Cultivars ◽  
Adequate Sampling ◽  
Pathogenic Variability

The pathogenic variability of isolates of R. secalis collected in Western Australia has been examined on different host genera of the Gramineae and on selected barley cultivars. Depending on the host-isolate combination and the conditions of the test, evidence has been obtained of inter- and intra-isolate variability in both host reaction and isolate pathogenicity. This complicates definitive interpretation of the results, militates against identification of conventional 'races' of the pathogen and shows that R. secalis does not exhibit strict host specialization. Hosts which consistently express resistance or susceptibility under different environmental conditions, and isolates which express their pathogenic characteristics consistently, have been identified. The need for more precise genetic studies and adequate sampling of genetic diversity is emphasized.

Xanthomonas axonopodis pv. mangiferaeindicae (mango black spot).

2021 ◽  
Author(s):  
Marion Doy
Keyword(s):  
Environmental Conditions ◽  
Black Spot ◽  
Xanthomonas Axonopodis ◽  
Commercial Cultivars

Abstract Several commercial cultivars of mango are very susceptible, and losses can be serious if environmental conditions are favourable (Willis, 2009; Pruvost et al., 2011a). On susceptible mango cultivars, namely Keitt, Kent and Tommy Atkins, trees may be weakened through loss of foliage, which eventually leads to lower yields (Kotzé and Visser, 1997).

scholarly journals Horizontal Transfer of Antimicrobial Resistance by Extended-Spectrum β Lactamase-Producing Enterobacteriaceae

2011 ◽  
Vol 3 (01) ◽  
pp. 037-042 ◽  
Author(s):  
Varsha K Vaidya
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Environmental Conditions ◽  
Horizontal Transfer ◽  
Hospital Setting ◽  
Alkaline Lysis ◽  
Antibiotic Resistant ◽  
Extended Spectrum ◽  
Synergy Test

ABSTRACT Background: The purpose of this work was to study the acquisition of new antibiotic-resistant genes carried by extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae via horizontal transfer to understand their rampant spread in the hospitals and in the community. Materials and Methods: A retrospective analysis of 120 ESBL screen-positive isolates of Escherichia coli and Klebsiella pneumoniae, which were subjected to antimicrobial susceptibility testing, was carried out. The Double Disc Synergy Test (DDST) and Inhibitor-Potentiation Disc Diffusion Test (IPDD) were employed for confirmation of ESBL activity. The transferability of the associated antibiotic resistance for amoxicillin, amikacin, gentamicin, cefotaxime and ceftriaxone was elucidated by intra- and intergenus conjugation in Escherichia coli under laboratory as well as under simulated environmental conditions. Transformation experiments using plasmids isolated by alkaline lysis method were performed to study the transferability of resistance genes in Klebsiella pneumoniae isolates. Results : ESBL production was indicated in 20% each of the Escherichia coli and Klebsiella pneumoniae isolates. All the ESBL isolates showed co- resistance to various other groups of antibiotics, including 3GC antibiotics, though all the isolates were sensitive to both the carbapenems tested. Conjugation-mediated transfer of resistance under laboratory as well as environmental conditions at a frequency of 3-4 x 10-5 , and transformation-mediated dissemination of cefotaxime and gentamicin resistance shed light on the propensity of ESBL producers for horizontal transfer. Conclusions: The transfer of resistant markers indicated availability of a large pool of resistance genes in the hospital setting as well as in the environment, facilitating long-term persistence of organisms.

scholarly journals Measuring the modal parameters of a cultural heritage tower by using strong-motion signals

ACTA IMEKO ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 86 ◽  
Author(s):  
Mariella Diaferio ◽  
Dora Foti ◽  
Nicola Ivan Giannoccaro ◽  
Salvador Ivorra Ivorra
Keyword(s):  
Phase Shift ◽  
Environmental Conditions ◽  
Excitation Frequency ◽  
Strong Motion ◽  
Forced Vibrations ◽  
Mode Shapes ◽  
Modal Parameters ◽  
Vibration Modes ◽  
Experimental Campaign ◽  
Different Levels

This paper presents the dynamic experimental campaign carried out on a stocky masonry clock tower situated in the Swabian Castle of Trani (Italy). The main objective of this paper is, after estimating the main frequencies and vibration modes of the considered structure, defining the transmission of vibrations along the height of the tower by varying the forced frequency at the base. At this aim, short acceleration records have been acquired simultaneously in 20 points of the tower at different levels, due to a series of sinusoidal forced vibrations applied at the base by using a pneumatic shaker device specify designed for the tests. The proposed procedure permit to extract for each monitored point the amplitude of the sinusoidal component related to the excitation frequency and the phase shift due to the structure damping. The results of the proposed procedure are compared with the results of a classical operational modal analysis in environmental conditions in order to demonstrate that the short forced tests permit to classify the typology of the structure mode shapes.

scholarly journals Genetic and no genetic variability of two-year old seedlings of 13 hybrid plane half-sib lines

2005 ◽  
pp. 69-85 ◽  
Author(s):  
Radmila Knezevic ◽  
Mirjana Sijacic-Nikolic
Keyword(s):  
Genetic Variability ◽  
Environmental Conditions ◽  
Hybrid Sterility ◽  
Soil Humidity ◽  
Frequent Mutations ◽  
Different Levels ◽  
Partial Hybrid

This paper presents the results of the analysis of variability of 8 analyzed morph metric parameters in two-year old seedlings of 13 half-sib lines at different levels. The recorded genetic and no genetic variability is conditioned by numerous different factors. The important causes of genetic variability are: partial incompatibility of parent trees, partial hybrid sterility, predominant inbreeding, frequent mutations with the growth of parent trees, very abundant gene recombination's, etc. The causes of no genetic seedling variability are: external environmental conditions (soil humidity, conditions of cultivation, sylvotechnical interventions, etc) and the internal or the somatic environment of seedling.

Phytophthora sojae pathotype distribution and fungicide sensitivity in Michigan

Plant Disease ◽  
2021 ◽  
Author(s):  
Austin Glenn McCoy ◽  
Zachary Albert Noel ◽  
Janette L Jacobs ◽  
Kayla M Clouse ◽  
Martin I Chilvers
Keyword(s):  
Resistance Genes ◽  
Phytophthora Sojae ◽  
Significant Shift ◽  
Seed Treatments ◽  
Fungicide Sensitivity ◽  
History Of ◽  
Plant Stand ◽  
Rps Gene ◽  
Major Resistance Genes ◽  
Pathogen Populations

Identifying the pathotype structure of a Phytophthora sojae population is crucial for the effective management of Phytophthora stem and root rot of soybean (PRR). P. sojae has been successfully managed with major resistance genes, partial resistance, and fungicide seed treatments. However, prolonged use of resistance genes or fungicides can cause pathogen populations to adapt over time, rendering resistance genes or fungicides ineffective. A statewide survey was conducted to characterize the current pathotype structure and fungicide sensitivity of P. sojae within Michigan. Soil samples were collected from 69 fields with a history of PRR and fields having consistent plant stand establishment issues. Eighty-three isolates of P. sojae were obtained, and hypocotyl inoculations were performed on 14 differential soybean cultivars, all of which carry a single Rps gene or no resistance gene. The survey identified a loss of effectiveness of Rps genes 1b, 1k, 3b and 6, compared to a previous survey conducted in Michigan from 1993-1997. Three effective resistance genes were identified for P. sojae management in Michigan; Rps 3a, 3c, and 4. Additionally, the effective concentration of common seed treatment fungicides to inhibit mycelial growth by 50% (EC50) was determined. No P. sojae isolates were insensitive to the tested chemistries with mean EC50 values of 2.60x10-2 µg/ml for ethaboxam, 3.03x10-2 µg/ml for mefenoxam, 2.88x10-4 µg/ml for oxathiapiprolin, and 5.08x10-2 µg/ml for pyraclostrobin. Results suggest that while there has been a significant shift in Rps gene effectiveness, seed treatments are still effective for early season management of this disease.

scholarly journals Common bean landraces as potential sources of resistance to anthracnose

2018 ◽  
Vol 48 (2) ◽  
pp. 126-133
Author(s):  
Ana Carolina da Costa Lara Fioreze ◽  
Sibila Grigolo ◽  
Claudia Aparecida Guginski Piva ◽  
Lays Sartori
Keyword(s):  
Environmental Conditions ◽  
Colletotrichum Lindemuthianum ◽  
Grain Production ◽  
Sources Of Resistance ◽  
Local Varieties ◽  
Commercial Cultivars ◽  
Potential Sources ◽  
Cropping Seasons ◽  
Moderately Resistant ◽  
Important Disease

ABSTRACT Anthracnose is an important disease caused by the Colletotrichum lindemuthianum (Sacc. & Magnus) fungus in bean crops. It occurs throughout Brazil and may cause losses of up to 100 % in grain production. About 20 genes that confer resistance to specific races of the pathogen have already been described in the literature. This study aimed to evaluate bean varieties from the Brazilian states of Santa Catarina and Paraná, regarding the resistance to anthracnose, in two cropping seasons. In addition, two commercial cultivars were used as controls: IPR Tangará, as susceptible; and BRS Campeiro, as moderately resistant. The majority of the local varieties was considered resistant or moderately resistant, being more pronounced the Amendoim cultivar, with large grains and red tegument, evincing resistance in both evaluations. Regarding this, the possibility of using them as resistance sources to anthracnose may be considered, since they also show adaptability to the environmental conditions.

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