scholarly journals Metabolic Stress is Not Sufficient to Increase Skeletal Muscle Tissue Extracellular Vesicle Secretion in an Ex Vivo System

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Zackary Valenti ◽  
Gabriella Hehn ◽  
Daniel Lark
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Ex Vivo ◽  
Metabolic Stress ◽  
Extracellular Vesicle ◽  
Skeletal Muscle Tissue ◽  
Vesicle Secretion

Extracellular vesicle secretion is tissue-dependent ex vivo and skeletal muscle myofiber extracellular vesicles reach the circulation in vivo.

2021 ◽  
Author(s):  
Andrea L. Estrada ◽  
Zackary J. Valenti ◽  
Gabriella Hehn ◽  
Adam J. Amorese ◽  
Nicholas S. Williams ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Vesicles ◽  
Direct Evidence ◽  
Ex Vivo ◽  
Extracellular Vesicle ◽  
Fluorescent Reporter ◽  
Human Protein Atlas ◽  
Health And Disease ◽  
Vesicle Secretion

Extracellular vesicles (EVs) are biomarkers and modifiers of human disease. EVs secreted by insulin-responsive tissues like skeletal muscle (SkM) and white adipose (WAT) contribute to metabolic health and disease but the relative abundance of EVs from these tissues has not been directly examined. Human Protein Atlas data and directly measuring EV secretion in mouse SkM and WAT using an ex vivo tissue explant model confirmed that SkM tissue secretes more EVs than WAT. Differences in EV secretion between SkM and WAT were not due to SkM contraction but may be explained by differences in tissue metabolic capacity. We next examined how many EVs secreted from SkM tissue ex vivo and in vivo are myofiber-derived. To do this, a SkM myofiber-specific dual fluorescent reporter mouse was created. Spectral flow cytometry revealed that SkM myofibers are a major source of SkM tissue-derived EVs ex vivo and EV immunocapture indicate that ~5% of circulating tetraspanin-positive EVs are derived from SkM myofibers in vivo. Our findings demonstrate that 1) SkM secretes more EVs than WAT, 2) many SkM tissue EVs are derived from SkM myofibers and 3) SkM myofiber-derived EVs reach the circulation in vivo. These findings advance our understanding of EV secretion between metabolically active tissues and provide direct evidence that SkM myofibers secrete EVs that can reach the circulation in vivo.

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

Diffusion of water in skeletal muscle tissue is not influenced by compression in a rat model of deep tissue injury

2010 ◽  
Vol 43 (3) ◽  
pp. 570-575 ◽  
Author(s):  
Bastiaan J. van Nierop ◽  
Anke Stekelenburg ◽  
Sandra Loerakker ◽  
Cees W. Oomens ◽  
Dan Bader ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Rat Model ◽  
Tissue Injury ◽  
Skeletal Muscle Tissue ◽  
Deep Tissue ◽  
Deep Tissue Injury

Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

2016 ◽  
Vol 95 (2) ◽  
pp. 341-347 ◽  
Author(s):  
YUUTA KOMATSU ◽  
SHIN SUKEGAWA ◽  
MAI YAMASHITA ◽  
NAOKI KATSUDA ◽  
BIN TONG ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Growth Rate ◽  
Differential Expression ◽  
Muscle Tissue ◽  
Expression Profile ◽  
Skeletal Muscle Tissue ◽  
Differential Expression Profile

Engineering vascularized skeletal muscle tissue

2005 ◽  
Vol 23 (7) ◽  
pp. 879-884 ◽  
Author(s):  
Shulamit Levenberg ◽  
Jeroen Rouwkema ◽  
Mara Macdonald ◽  
Evan S Garfein ◽  
Daniel S Kohane ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue
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