scholarly journals Non‐invasive stool‐based detection of newborn infant gastrointestinal development using gene expression profiles derived from exfoliated epithelial cells

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Sharon Marie Donovan ◽  
Laure A Davidson ◽  
Chen Zhao ◽  
Ivan Ivanov ◽  
Jennifer S Goldsby ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Newborn Infant ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Non Invasive

Spatial Transcriptomics analysis of uterine gene expression in enhancer of Zeste homolog 2 (Ezh2) conditional knockout mice

2021 ◽  
Author(s):  
Ana M Mesa ◽  
Jiude Mao ◽  
Theresa I Medrano ◽  
Nathan J Bivens ◽  
Alexander Jurkevich ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Expression Profiles ◽  
Reproductive Organs ◽  
Conditional Knockout ◽  
Estrogen Signaling ◽  
Uterine Epithelial Cell ◽  
Stromal Tissue

Abstract Histone proteins undergo various modifications that alter chromatin structure, including addition of methyl groups. Enhancer of homolog 2 (EZH2), is a histone methyltransferase that methylates lysine residue 27, and thereby, suppresses gene expression. EZH2 plays integral role in the uterus and other reproductive organs. We have previously shown that conditional deletion of uterine EZH2 results in increased proliferation of luminal and glandular epithelial cells, and RNAseq analyses reveal several uterine transcriptomic changes in Ezh2 conditional (c) knockout (KO) mice that can affect estrogen signaling pathways. To pinpoint the origin of such gene expression changes, we used the recently developed spatial transcriptomics (ST) method with the hypotheses that Ezh2cKO mice would predominantly demonstrate changes in epithelial cells and/or ablation of this gene would disrupt normal epithelial/stromal gene expression patterns. Uteri were collected from ovariectomized adult WT and Ezh2cKO mice and analyzed by ST. Asb4, Cxcl14, Dio2, and Igfbp5 were increased, Sult1d1, Mt3, and Lcn2 were reduced in Ezh2cKO uterine epithelium vs. WT epithelium. For Ezh2cKO uterine stroma, differentially expressed key hub genes included Cald1, Fbln1, Myh11, Acta2, and Tagln. Conditional loss of uterine Ezh2 also appears to shift the balance of gene expression profiles in epithelial vs. stromal tissue toward uterine epithelial cell and gland development and proliferation, consistent with uterine gland hyperplasia in these mice. Current findings provide further insight into how EZH2 may selectively affect uterine epithelial and stromal compartments. Additionally, these transcriptome data might provide the mechanistic understanding and valuable biomarkers for human endometrial disorders with epigenetic underpinnings.

Effect of tobacco compounds on gene expression profiles in human epithelial cells

2009 ◽  
Vol 27 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Sung-Hwa Sohn ◽  
Jaebum Lee ◽  
Ki-Nam Kim ◽  
In kyoung Kim ◽  
Meyoung-Kon Kim
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Epithelial Cells

scholarly journals Gene Expression in Porphyromonas gingivalis after Contact with Human Epithelial Cells

2005 ◽  
Vol 73 (4) ◽  
pp. 2327-2335 ◽  
Author(s):  
Yumiko Hosogi ◽  
Margaret J. Duncan
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cell Types ◽  
Adverse Conditions ◽  
Human Epithelial Cells ◽  
Host Epithelium ◽  
Shock Proteins

ABSTRACT Porphyromonas gingivalis, a gram-negative oral anaerobe, is strongly associated with adult periodontitis. The adherence of the organism to host epithelium signals changes in both cell types as bacteria initiate infection and colonization and epithelial cells rally their defenses. We hypothesized that the expression of a defined set of P. gingivalis genes would be consistently up-regulated during infection of HEp-2 human epithelial cells. P. gingivalis genome microarrays were used to compare the gene expression profiles of bacteria that adhered to HEp-2 cells and bacteria that were incubated alone. Genes whose expression was temporally up-regulated included those involved in the oxidative stress response and those encoding heat shock proteins that are essential to maintaining cell viability under adverse conditions. The results suggest that contact with epithelial cells induces in P. gingivalis stress-responsive pathways that promote the survival of the bacterium.

scholarly journals Comparison of Gene Expression Profiles of Conjunctival Cell Lines With Primary Cultured Conjunctival Epithelial Cells and Human Conjunctival Tissue

2009 ◽  
Vol 14 (5) ◽  
pp. 265-278 ◽  
Author(s):  
LOUIS TONG ◽  
YOLANDA DIEBOLD ◽  
MARGARITA CALONGE ◽  
JIANPING GAO ◽  
MICHAEL E. STERN ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Conjunctival Epithelial Cells
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