scholarly journals The Role of Melanin Concentrating Hormone in Central Chemoreception: a Knock‐down Study by siRNAs in the Lateral Hypothalamus

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Ningjing Li ◽  
Aihua Li ◽  
Eugene Nattie
Keyword(s):  
Lateral Hypothalamus ◽  
Knock Down ◽  
Melanin Concentrating Hormone ◽  
Central Chemoreception

scholarly journals Paternally expressed imprinted Snord116 and Peg3 regulate hypothalamic orexin neurons

2019 ◽  
Author(s):  
Pace Marta ◽  
Falappa Matteo ◽  
Freschi Andrea ◽  
Balzani Edoardo ◽  
Berteotti Chiara ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Lateral Hypothalamus ◽  
Rem Sleep ◽  
Neurodevelopmental Disorder ◽  
Imprinted Genes ◽  
List Type ◽  
State Dependent ◽  
Sleep Homeostasis ◽  
Melanin Concentrating Hormone

AbstractImprinted genes are highly expressed in the hypothalamus; however, whether specific imprinted genes affect hypothalamic neuromodulators and their functions is unknown. It has been suggested that Prader-Willi syndrome (PWS), a neurodevelopmental disorder caused by lack of paternal expression at chromosome 15q11-q13, is characterised by hypothalamic insufficiency. Here, we investigate the role of the paternally expressed Snord116 gene within the context of sleep and metabolic abnormalities of PWS, and we report a novel role of this imprinted gene in the function and organisation of the two main neuromodulatory systems of the lateral hypothalamus (LH), namely, the orexin (OX) and melanin concentrating hormone (MCH) systems. We observe that the dynamics between neuronal discharge in the LH and the sleep-wake states of mice with paternal deletion of Snord116 (PWScrm+/p−) are compromised. This abnormal state-dependent neuronal activity is paralleled by a significant reduction in OX neurons in the LH of mutants. Therefore, we propose that an imbalance between OX- and MCH-expressing neurons in the LH of mutants reflects a series of deficits manifested in the PWS, such as dysregulation of rapid eye movement (REM) sleep, food intake and temperature control.HighlightsSnord116 regulates neuronal activity in the lateral hypothalamus (LH), which is time-locked with cortical states of sleep.Loss of Snord116 reduces orexin neurons in the LH and affects sleep homeostasis and thermoregulation in mice.Snord116 and Peg3 independently control orexin expression in the LH.Paternally expressed alleles maximize the patrilineal effects in the control of REM sleep by the LH in mammals.

scholarly journals Glutamatergic Input to the Lateral Hypothalamus Stimulates Ethanol Intake: Role of Orexin and Melanin-Concentrating Hormone

2012 ◽  
Vol 37 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Yu-Wei Chen ◽  
Jessica R. Barson ◽  
Aimee Chen ◽  
Bartley G. Hoebel ◽  
Sarah F. Leibowitz
Keyword(s):  
Lateral Hypothalamus ◽  
Ethanol Intake ◽  
Melanin Concentrating Hormone

scholarly journals CRISPR/Cas9 Mediated Knock Down of δ-ENaC Blunted the TNF-Induced Activation of ENaC in A549 Cells

2021 ◽  
Vol 22 (4) ◽  
pp. 1858
Author(s):  
Waheed Shabbir ◽  
Nermina Topcagic ◽  
Mohammed Aufy ◽  
Murat Oz
Keyword(s):  
A549 Cells ◽  
Na Channel ◽  
Na Current ◽  
Patch Clamp Technique ◽  
Whole Cell ◽  
Knock Down ◽  
Whole Cell Patch Clamp ◽  
Glycosylation Sites ◽  
Epithelial Na Channel

Tumor necrosis factor (TNF) is known to activate the epithelial Na+ channel (ENaC) in A549 cells. A549 cells are widely used model for ENaC research. The role of δ-ENaC subunit in TNF-induced activation has not been studied. In this study we hypothesized that δ-ENaC plays a major role in TNF-induced activation of ENaC channel in A549 cells which are widely used model for ENaC research. We used CRISPR/Cas 9 approach to knock down (KD) the δ-ENaC in A549 cells. Western blot and immunofluorescence assays were performed to analyze efficacy of δ-ENaC protein KD. Whole-cell patch clamp technique was used to analyze the TNF-induced activation of ENaC. Overexpression of wild type δ-ENaC in the δ-ENaC KD of A549 cells restored the TNF-induced activation of whole-cell Na+ current. Neither N-linked glycosylation sites nor carboxyl terminus domain of δ-ENaC was necessary for the TNF-induced activation of whole-cell Na+ current in δ-ENaC KD of A549 cells. Our data demonstrated that in A549 cells the δ-ENaC plays a major role in TNF-induced activation of ENaC.

Role of lateral hypothalamus on fluid, electrolyte, and cardiovascular responses to activation of the MSA

1994 ◽  
Vol 266 (2) ◽  
pp. R496-R502 ◽  
Author(s):  
A. S. Haibara ◽  
W. A. Saad ◽  
J. V. Menani ◽  
L. A. Camargo ◽  
A. Renzi
Keyword(s):  
Water Intake ◽  
Lateral Hypothalamus ◽  
Cardiovascular Responses ◽  
Electrolytic Lesion ◽  
Opioid Agonist ◽  
Inhibitory Mechanisms ◽  
Pressor Responses ◽  
Medial Septal Area ◽  
Opiate Agonist

In this study we investigated the influence of electrolytic lesion or of opioid agonist injections into the lateral hypothalamus (LH) on the dipsogenic, natriuretic, kaliuretic, antidiuretic, pressor, and bradycardiac effects of cholinergic stimulation of the medial septal area (MSA) in rats. Sham- and LH-lesioned male Holtzman rats received a stainless steel cannula implanted into the LH. Other groups of rats had cannulas implanted simultaneously into the MSA and LH. Carbachol (2 nmol) injection into the MSA induced water intake, pressor, and bradycardic responses. LH lesion reduced all of these effects (1-3 and 15-18 days). Previous injection of synthetic opiate agonist, FK-33824 (100 ng), into the LH reduced the water intake, natriuresis, kaliuresis, and pressor responses induced by carbachol injected into the MSA. These data show that both electrolytic lesion or injection of an opiate agonist in the LH reduces the fluid-electrolyte and cardiovascular responses to cholinergic activation of the MSA. The involvement of LH with central excitatory and inhibitory mechanisms related to fluid-electrolytic and cardiovascular control is suggested.

2-AG into the lateral hypothalamus increases REM sleep and cFos expression in melanin concentrating hormone neurons in rats

2013 ◽  
Vol 108 ◽  
pp. 1-7 ◽  
Author(s):  
Marcel Pérez-Morales ◽  
Alberto K. De La Herrán-Arita ◽  
Mónica Méndez-Díaz ◽  
Alejandra E. Ruiz-Contreras ◽  
René Drucker-Colín ◽  
...  
Keyword(s):  
Lateral Hypothalamus ◽  
Rem Sleep ◽  
Melanin Concentrating Hormone

Neurotensin Enhances Locomotor Activity and Arousal and Inhibits Melanin-Concentrating Hormone Signaling

2019 ◽  
Vol 110 (1-2) ◽  
pp. 35-49 ◽  
Author(s):  
Talia Levitas-Djerbi ◽  
Dana Sagi ◽  
Ilana Lebenthal-Loinger ◽  
Tali Lerer-Goldshtein ◽  
Lior Appelbaum
Keyword(s):  
Locomotor Activity ◽  
Hormone Receptor ◽  
Behavioral Response ◽  
Hormone Signaling ◽  
Wild Type ◽  
Expression Levels ◽  
Physiological Processes ◽  
Melanin Concentrating Hormone ◽  
Behavioral Assays

Background: Hypothalamic neurotensin (Nts)-secreting neurons regulate fundamental physiological processes including metabolism and feeding. However, the role of Nts in modulation of locomotor activity, sleep, and arousal is unclear. We previously identified and characterized Nts neurons in the zebrafish hypothalamus. Materials and Methods: In order to study the role of Nts, nts mutant (nts–/–), and overexpressing zebrafish were generated. Results: The expression of both nts mRNA and Nts protein was reduced during the night in wild-type zebrafish. Behavioral assays revealed that locomotor activity was decreased during both day and night, while sleep was increased exclusively during the nighttime in nts–/– larvae. Likewise, inducible overexpression of Nts increased arousal in hsp70:Gal4/uas:Nts larvae. Furthermore, the behavioral response to light-to-dark transitions was reduced in nts–/– larvae. In order to elucidate potential contenders that may mediate Nts action on these behaviors, we profiled the transcriptome of 6 dpf nts–/– larvae. Among other genes, the expression levels of melanin-concentrating hormone receptor 1b were increased in nts–/– larvae. Furthermore, a portion of promelanin-concentrating hormone 1 (pmch1) and pmch2 neurons expressed the nts receptor. In addition, expression of the the two zebrafish melanin-concentrating hormone (Mch) orthologs, Mch1 and Mch2, was increased in nts–/– larvae. Conclusion: These results show that the Nts and Mch systems interact and modulate locomotor activity and arousal.

Hegel on the Nature of Scepticism

2011 ◽  
Vol 32 (1-2) ◽  
pp. 80-99 ◽  
Author(s):  
Dietmar H. Heidemann
Keyword(s):  
The Other ◽  
Human Cognition ◽  
Philosophical Method ◽  
Philosophical Thinking ◽  
Knock Down ◽  
Human Understanding ◽  
Early View ◽  
The One ◽  
Intrinsic Feature

In the Encyclopaedia Logic, Hegel states that ‘philosophy … contains the sceptical as a moment within itself — specifically as the dialectical moment’ (§81, Addition 2), and that ‘scepticism’ as ‘the dialectical moment itself is an essential one in the affirmative Science’ (§78). On the one hand, the connection between scepticism and dialectic is obvious. Hegel claims that scepticism is a problem that cannot be just removed from the philosophical agenda by knock-down anti-sceptical arguments. Scepticism intrinsically belongs to philosophical thinking; that is to say, it plays a constructive role in philosophical thinking. On the other hand, scepticism has to be construed as the view according to which we cannot know whether our beliefs are true, i.e., scepticism plays a destructive role in philosophy no matter what. It is particularly this role that clashes with Hegel's claim of having established a philosophical system of true cognition of the entirety of reality. In the following I argue that for Hegel the constructive and the destructive role of scepticism are reconcilable. I specifically argue that it is dialectic that makes both consistent since scepticism is a constitutive element of dialectic.In order to show in what sense scepticism is an intrinsic feature of dialectic I begin by sketching Hegel's early view of scepticism specifically with respect to logic and metaphysics. The young Hegel construes logic as a philosophical method of human cognition that inevitably results in ‘sceptical’ consequences in that it illustrates the finiteness of human understanding. By doing so, logic not only nullifies finite understanding but also introduces to metaphysics, i.e., the true philosophical science of the absolute.

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