scholarly journals B‐lymphocyte–intrinsic and –extrinsic defects in secretory immunoglobulin A production in the neural crest–conditional deletion of endothelin receptor B model of Hirschsprung‐associated enterocolitis

2019 ◽  
Vol 33 (6) ◽  
pp. 7615-7624 ◽  
Author(s):  
Giuliana Medrano ◽  
Frederic Cailleux ◽  
Peihong Guan ◽  
Korah Kuruvilla ◽  
Amanda J. Barlow-Anacker ◽  
...  
Keyword(s):  
Neural Crest ◽  
B Lymphocyte ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
Endothelin Receptor ◽  
Conditional Deletion ◽  
Endothelin Receptor B ◽  
Extrinsic Defects ◽  
Secretory Immunoglobulin

scholarly journals Impaired Cellular Immunity in the Murine Neural Crest Conditional Deletion of Endothelin Receptor-B Model of Hirschsprung’s Disease

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128822 ◽  
Author(s):  
Ankush Gosain ◽  
Amanda J. Barlow-Anacker ◽  
Chris S. Erickson ◽  
Joseph F. Pierre ◽  
Aaron F. Heneghan ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cellular Immunity ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Endothelin Receptor ◽  
Conditional Deletion ◽  
Endothelin Receptor B

scholarly journals The immune landscape of IgA induction in the gut

2021 ◽  
Author(s):  
Claudia Seikrit ◽  
Oliver Pabst
Keyword(s):  
Protective Immunity ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Dark Side ◽  
Secretory Immunoglobulin A ◽  
Antibody Isotype ◽  
Mucosal Antibody ◽  
Key Concepts ◽  
Basic Understanding ◽  
Secretory Immunoglobulin

AbstractAntibodies are key elements of protective immunity. In the mucosal immune system in particular, secretory immunoglobulin A (SIgA), the most abundantly produced antibody isotype, protects against infections, shields the mucosal surface from toxins and environmental factors, and regulates immune homeostasis and a peaceful coexistence with our microbiota. However, the dark side of IgA biology promotes the formation of immune complexes and provokes pathologies, e.g., IgA nephropathy (IgAN). The precise mechanisms of how IgA responses become deregulated and pathogenic in IgAN remain unresolved. Yet, as the field of microbiota research moved into the limelight, our basic understanding of IgA biology has been taking a leap forward. Here, we discuss the structure of IgA, the anatomical and cellular foundation of mucosal antibody responses, and current concepts of how we envision the interaction of SIgA and the microbiota. We center on key concepts in the field while taking account of both historic findings and exciting new observations to provide a comprehensive groundwork for the understanding of IgA biology from the perspective of a mucosal immunologist.

scholarly journals Fecal Antibodies to Cryptosporidium parvum in Healthy Volunteers

2000 ◽  
Vol 68 (9) ◽  
pp. 5068-5074 ◽  
Author(s):  
Sara M. Dann ◽  
Pablo C. Okhuysen ◽  
Bassam M. Salameh ◽  
Herbert L. DuPont ◽  
Cynthia L. Chappell
Keyword(s):  
Antibody Response ◽  
Healthy Volunteers ◽  
Cryptosporidium Parvum ◽  
Immunoglobulin A ◽  
Reactivity Index ◽  
Secretory Immunoglobulin A ◽  
Low Doses ◽  
Previous Exposure ◽  
Oocyst Excretion ◽  
Secretory Immunoglobulin

ABSTRACT This study examined the intestinal antibody response in 26 healthy volunteers challenged with Cryptosporidium parvum oocysts. Fecal extracts were assayed for total secretory immunoglobulin A (IgA) and C. parvum-specific IgA reactivity. Specific IgA reactivity was standardized to IgA concentration and expressed as a reactivity index (RI). Anti-C. parvum fecal IgA (fIgA) increased significantly in 17 of 26 (65.4%) following oocyst ingestion. Of those with detectable responses, 59, 76.5, and 94.1% were positive by days 7, 14, and 30, respectively. Volunteers receiving high challenge doses (>1,000 and 300 to 500 oocysts) had higher RIs (RI = 5.57 [P = 0.027] and RI = 1.68 [P = 0.039], respectively) than those ingesting low doses (30 to 100 oocysts; RI = 0.146). Subjects shedding oocysts and experiencing a diarrheal illness had the highest fIgA reactivity. When evaluated separately, oocyst excretion was associated with an increased fIgA response compared to nonshedders (RI = 1.679 versus 0.024, respectively; P = 0.003). However, in subjects experiencing diarrhea with or without oocyst shedding, a trend toward a higher RI (P = 0.065) was seen. Extracts positive for fecal IgA were further examined for IgA subclass. The majority of stools contained both IgA1 and IgA2, and the relative proportions did not change following challenge. Also, no C. parvum-specific IgM or IgG was detected in fecal extracts. Thus, fecal IgA to C. parvum antigens was highly associated with infection in subjects who had no evidence of previous exposure and may provide a useful tool in detecting recent infections.

Sign in / Sign up

Export Citation Format

Share Document